immpact vip peroxidase hrp substrate (Vector Laboratories)
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Name:
ImmPACT VIP Peroxidase HRP Substrate
Description:
ImmPACT VIP Peroxidase Substrate produces an intense violet purple reaction products and can be used as alternatives to DAB or as a second color for multiple antigen labeling Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina 1 Features 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsPermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and stock solutions in dropper bottles With the aid of imaging systems and software the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co localized Sections stained with either substrate can be viewed by darkfield and electron microscopy Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods Stained sections should be dehydrated cleared and permanently mounted This product utilizes Vector Laboratories novel ImmPACT technology to generate an intense violet reaction product that is 2 4 times more sensitive than Vector VIP substrate kit SK 4600 This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4 °C
Catalog Number:
sk-4605
Price:
None
Category:
Protein chromogenic detection reagents or kits or substrates
Size:
120 ml
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Structured Review
Vector Laboratories
immpact vip peroxidase hrp substrate
ImmPACT VIP Peroxidase Substrate produces an intense violet purple reaction products and can be used as alternatives to DAB or as a second color for multiple antigen labeling Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina 1 Features 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsPermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and stock solutions in dropper bottles With the aid of imaging systems and software the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co localized Sections stained with either substrate can be viewed by darkfield and electron microscopy Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods Stained sections should be dehydrated cleared and permanently mounted This product utilizes Vector Laboratories novel ImmPACT technology to generate an intense violet reaction product that is 2 4 times more sensitive than Vector VIP substrate kit SK 4600 This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4 °C
https://www.bioz.com/result/immpact vip peroxidase hrp substrate/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ImmPACT VIP Peroxidase Substrate produces an intense violet purple reaction products and can be used as alternatives to DAB or as a second color for multiple antigen labeling Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina 1 Features 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsPermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and stock solutions in dropper bottles With the aid of imaging systems and software the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co localized Sections stained with either substrate can be viewed by darkfield and electron microscopy Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods Stained sections should be dehydrated cleared and permanently mounted This product utilizes Vector Laboratories novel ImmPACT technology to generate an intense violet reaction product that is 2 4 times more sensitive than Vector VIP substrate kit SK 4600 This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4 °C
https://www.bioz.com/result/immpact vip peroxidase hrp substrate/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
immpact vip peroxidase hrp substrate - by Bioz Stars,
2021-03
93/100 stars
Images
1) Product Images from "Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning"
Article Title: Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning
Journal: Nature biomedical engineering
doi: 10.1038/s41551-018-0265-3
Figure Legend Snippet: Assay validation a , Different chromogenic substrates were tested to reveal Ki67 in lymphoma cell lines. Among those tested, the ImmPACT VIP substrate showed the greatest contrast between stained and unstained control samples. b , The CEM measured marker expressions of kappa light chain, lambda light chain and Ki67 for three different cell lines (Daudi, DB and Jurkat) and compared to marker expressions measured by gold-standard flow cytometry. Note the congruency. c , Ki67 Antibodies were lyophilized and stored at different temperatures. After rehydration, the antibodies and the CEM assay showed good reproducibility with a standard variation of
Techniques Used: Staining, Marker, Flow Cytometry, Cytometry
Related Articles
Staining:Article Title: Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning Article Snippet: Labeled cells were introduced into flow cells and incubated for 30 min. B-cells labeled with biotinylated anti-CD19/CD20 antibodies were captured on the neutravidin-coated surface of flow cells, and unbound cells were removed by washing at 40 mL/h for 1 mL. .. Captured cells were stained with Article Title: Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis Article Snippet: Primary antibodies were F4/80 (#MCA497; Bio-Rad, Hercules, CA, USA) and CD39 for IF (C9F, kind gift from Jean Sévigny, Centre de Recherche du CHU de Québec, Université Laval, Quebec, Canada), as well as CD39 (#AF-4398; R & D Systems, Minneapolis, MN, USA) and pan-Cytokeratin for IHC (#Z0622; Dako, Agilent Technologies, Santa Clara, CA, USA). .. In DDC-fed mice, staining was developed using Article Title: Biological Cardiac Tissue Effects of High-Energy Heavy Ions – Investigation for Myocardial Ablation Article Snippet: Primary antibodies used are anti-Troponin T cardiac isoform (Thermo Scientific); anti-CD45, α-SMA, anti-Cytokeratin 10 and anti-Ki 67 (all from Abcam). .. For detection of binding of the primary antibody, the Ultra-Sensitive ABC Peroxidase rabbit/mouse IgG staining kit (Thermo Scientific, Waltham, MA, USA) and the Article Title: Allosteric and ATP-Competitive Inhibitors of mTOR Effectively Suppress Tumor Progression-Associated Epithelial-Mesenchymal Transition in the Kidneys of Tsc2+/− Mice Article Snippet: For MS-IHC, previous IHC-stained slides were incubated in xylene for 10 minutes to remove coverslips and then incubated at 50°C in a buffered solution containing 5% SDS, 0.5% mercaptoethanol, and 50 mm Tris–HCl (pH 7.5) for 60 minutes to strip primary antibodies, and finally, the protocol was followed for conventional IHC. .. SignalStain Boost Rabbit specific IHC Detection Reagent (Cell Signaling Technology, Danvers, MA) and ImmPACT NovaRED Peroxidase Substrate or Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the Mouse Assay:Article Title: Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis Article Snippet: Primary antibodies were F4/80 (#MCA497; Bio-Rad, Hercules, CA, USA) and CD39 for IF (C9F, kind gift from Jean Sévigny, Centre de Recherche du CHU de Québec, Université Laval, Quebec, Canada), as well as CD39 (#AF-4398; R & D Systems, Minneapolis, MN, USA) and pan-Cytokeratin for IHC (#Z0622; Dako, Agilent Technologies, Santa Clara, CA, USA). .. In DDC-fed mice, staining was developed using Binding Assay:Article Title: Biological Cardiac Tissue Effects of High-Energy Heavy Ions – Investigation for Myocardial Ablation Article Snippet: Primary antibodies used are anti-Troponin T cardiac isoform (Thermo Scientific); anti-CD45, α-SMA, anti-Cytokeratin 10 and anti-Ki 67 (all from Abcam). .. For detection of binding of the primary antibody, the Ultra-Sensitive ABC Peroxidase rabbit/mouse IgG staining kit (Thermo Scientific, Waltham, MA, USA) and the Incubation:Article Title: Efficient in vivo editing of OTC-deficient patient-derived primary human hepatocytes Article Snippet: Sections were incubated for 45 min at room temperature with biotin-conjugated secondary antibodies: donkey anti-sheep IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, 713-065-147, 1:600 dilution) or donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 711-065-152, 1:800 dilution). .. Antigens were visualised by chromogenic detection using RTU Elite ABC reagent (Vector Laboratories, Burlingame, CA) and subsequent incubation with one the following substrates: ImmPACT™ DAB peroxidase substrate (Vector Laboratories) or Immunohistochemistry:Article Title: Allosteric and ATP-Competitive Inhibitors of mTOR Effectively Suppress Tumor Progression-Associated Epithelial-Mesenchymal Transition in the Kidneys of Tsc2+/− Mice Article Snippet: For MS-IHC, previous IHC-stained slides were incubated in xylene for 10 minutes to remove coverslips and then incubated at 50°C in a buffered solution containing 5% SDS, 0.5% mercaptoethanol, and 50 mm Tris–HCl (pH 7.5) for 60 minutes to strip primary antibodies, and finally, the protocol was followed for conventional IHC. .. SignalStain Boost Rabbit specific IHC Detection Reagent (Cell Signaling Technology, Danvers, MA) and ImmPACT NovaRED Peroxidase Substrate or TUNEL Assay:Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the Marker:Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the Activity Assay:Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the |