immpact vip peroxidase hrp substrate  (Vector Laboratories)


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    Name:
    ImmPACT VIP Peroxidase HRP Substrate
    Description:
    ImmPACT VIP Peroxidase Substrate produces an intense violet purple reaction products and can be used as alternatives to DAB or as a second color for multiple antigen labeling Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina 1 Features 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsPermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and stock solutions in dropper bottles With the aid of imaging systems and software the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co localized Sections stained with either substrate can be viewed by darkfield and electron microscopy Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods Stained sections should be dehydrated cleared and permanently mounted This product utilizes Vector Laboratories novel ImmPACT technology to generate an intense violet reaction product that is 2 4 times more sensitive than Vector VIP substrate kit SK 4600 This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4 °C
    Catalog Number:
    sk-4605
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Size:
    120 ml
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    Structured Review

    Vector Laboratories immpact vip peroxidase hrp substrate
    ImmPACT VIP Peroxidase HRP Substrate
    ImmPACT VIP Peroxidase Substrate produces an intense violet purple reaction products and can be used as alternatives to DAB or as a second color for multiple antigen labeling Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina 1 Features 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsPermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and stock solutions in dropper bottles With the aid of imaging systems and software the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co localized Sections stained with either substrate can be viewed by darkfield and electron microscopy Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods Stained sections should be dehydrated cleared and permanently mounted This product utilizes Vector Laboratories novel ImmPACT technology to generate an intense violet reaction product that is 2 4 times more sensitive than Vector VIP substrate kit SK 4600 This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4 °C
    https://www.bioz.com/result/immpact vip peroxidase hrp substrate/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpact vip peroxidase hrp substrate - by Bioz Stars, 2021-03
    93/100 stars

    Images

    1) Product Images from "Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning"

    Article Title: Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning

    Journal: Nature biomedical engineering

    doi: 10.1038/s41551-018-0265-3

    Assay validation a , Different chromogenic substrates were tested to reveal Ki67 in lymphoma cell lines. Among those tested, the ImmPACT VIP substrate showed the greatest contrast between stained and unstained control samples. b , The CEM measured marker expressions of kappa light chain, lambda light chain and Ki67 for three different cell lines (Daudi, DB and Jurkat) and compared to marker expressions measured by gold-standard flow cytometry. Note the congruency. c , Ki67 Antibodies were lyophilized and stored at different temperatures. After rehydration, the antibodies and the CEM assay showed good reproducibility with a standard variation of
    Figure Legend Snippet: Assay validation a , Different chromogenic substrates were tested to reveal Ki67 in lymphoma cell lines. Among those tested, the ImmPACT VIP substrate showed the greatest contrast between stained and unstained control samples. b , The CEM measured marker expressions of kappa light chain, lambda light chain and Ki67 for three different cell lines (Daudi, DB and Jurkat) and compared to marker expressions measured by gold-standard flow cytometry. Note the congruency. c , Ki67 Antibodies were lyophilized and stored at different temperatures. After rehydration, the antibodies and the CEM assay showed good reproducibility with a standard variation of

    Techniques Used: Staining, Marker, Flow Cytometry, Cytometry

    Related Articles

    Staining:

    Article Title: Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning
    Article Snippet: Labeled cells were introduced into flow cells and incubated for 30 min. B-cells labeled with biotinylated anti-CD19/CD20 antibodies were captured on the neutravidin-coated surface of flow cells, and unbound cells were removed by washing at 40 mL/h for 1 mL. .. Captured cells were stained with Immpact VIP Peroxidase (HRP) substrate (Vector Labs) for 15 min. Three images per sample (pre-wash, post-wash and stained) were taken using the CEM device. ..

    Article Title: Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis
    Article Snippet: Primary antibodies were F4/80 (#MCA497; Bio-Rad, Hercules, CA, USA) and CD39 for IF (C9F, kind gift from Jean Sévigny, Centre de Recherche du CHU de Québec, Université Laval, Quebec, Canada), as well as CD39 (#AF-4398; R & D Systems, Minneapolis, MN, USA) and pan-Cytokeratin for IHC (#Z0622; Dako, Agilent Technologies, Santa Clara, CA, USA). .. In DDC-fed mice, staining was developed using ImmPACT VIP Peroxidase (#SK-4605, Vector Laboratories, Burlingame, CA), which results in a purple reaction product distinct from the brownish porphyrine plugs present in DDC-fed livers. .. In all other animals, ImmPACT DAB Peroxidase (#SK-4100, Vector Laboratories) for brown color development was used.

    Article Title: Biological Cardiac Tissue Effects of High-Energy Heavy Ions – Investigation for Myocardial Ablation
    Article Snippet: Primary antibodies used are anti-Troponin T cardiac isoform (Thermo Scientific); anti-CD45, α-SMA, anti-Cytokeratin 10 and anti-Ki 67 (all from Abcam). .. For detection of binding of the primary antibody, the Ultra-Sensitive ABC Peroxidase rabbit/mouse IgG staining kit (Thermo Scientific, Waltham, MA, USA) and the ImmPACT VIP-Peroxidase substrate kit (Vector, Burlingame, CA, USA) were used according to the respective manufacturer’s protocols. .. Nuclei were counterstained with hematoxylin and slides were dehydrated, cleared in xylene, and mounted with Eukitt (Sigma Aldrich, USA).

    Article Title: Allosteric and ATP-Competitive Inhibitors of mTOR Effectively Suppress Tumor Progression-Associated Epithelial-Mesenchymal Transition in the Kidneys of Tsc2+/− Mice
    Article Snippet: For MS-IHC, previous IHC-stained slides were incubated in xylene for 10 minutes to remove coverslips and then incubated at 50°C in a buffered solution containing 5% SDS, 0.5% mercaptoethanol, and 50 mm Tris–HCl (pH 7.5) for 60 minutes to strip primary antibodies, and finally, the protocol was followed for conventional IHC. .. SignalStain Boost Rabbit specific IHC Detection Reagent (Cell Signaling Technology, Danvers, MA) and ImmPACT NovaRED Peroxidase Substrate or ImmPACT VIP Peroxidase (HRP) Substrate (Vector Laboratories, Peterborough, UK) were used to stain antigens according to the kit suppliers' instruction. .. IHC or MS-IHC stained slides were scanned to generate virtual slides for photo capture using an Aperio system.

    Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis
    Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the ImmPACT VIP Peroxidase Substrate kit (Vector Laboratories). .. This kit produces a purple product that is easily distinguished from the brown product of the TUNEL staining.

    Mouse Assay:

    Article Title: Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis
    Article Snippet: Primary antibodies were F4/80 (#MCA497; Bio-Rad, Hercules, CA, USA) and CD39 for IF (C9F, kind gift from Jean Sévigny, Centre de Recherche du CHU de Québec, Université Laval, Quebec, Canada), as well as CD39 (#AF-4398; R & D Systems, Minneapolis, MN, USA) and pan-Cytokeratin for IHC (#Z0622; Dako, Agilent Technologies, Santa Clara, CA, USA). .. In DDC-fed mice, staining was developed using ImmPACT VIP Peroxidase (#SK-4605, Vector Laboratories, Burlingame, CA), which results in a purple reaction product distinct from the brownish porphyrine plugs present in DDC-fed livers. .. In all other animals, ImmPACT DAB Peroxidase (#SK-4100, Vector Laboratories) for brown color development was used.

    Binding Assay:

    Article Title: Biological Cardiac Tissue Effects of High-Energy Heavy Ions – Investigation for Myocardial Ablation
    Article Snippet: Primary antibodies used are anti-Troponin T cardiac isoform (Thermo Scientific); anti-CD45, α-SMA, anti-Cytokeratin 10 and anti-Ki 67 (all from Abcam). .. For detection of binding of the primary antibody, the Ultra-Sensitive ABC Peroxidase rabbit/mouse IgG staining kit (Thermo Scientific, Waltham, MA, USA) and the ImmPACT VIP-Peroxidase substrate kit (Vector, Burlingame, CA, USA) were used according to the respective manufacturer’s protocols. .. Nuclei were counterstained with hematoxylin and slides were dehydrated, cleared in xylene, and mounted with Eukitt (Sigma Aldrich, USA).

    Incubation:

    Article Title: Efficient in vivo editing of OTC-deficient patient-derived primary human hepatocytes
    Article Snippet: Sections were incubated for 45 min at room temperature with biotin-conjugated secondary antibodies: donkey anti-sheep IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, 713-065-147, 1:600 dilution) or donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 711-065-152, 1:800 dilution). .. Antigens were visualised by chromogenic detection using RTU Elite ABC reagent (Vector Laboratories, Burlingame, CA) and subsequent incubation with one the following substrates: ImmPACT™ DAB peroxidase substrate (Vector Laboratories) or ImmPACT™ VIP (VectorLabs) for BrdU or GAPDH, respectively. .. Western blot analysis Western blot was performed on liver lysates as previously described.

    Immunohistochemistry:

    Article Title: Allosteric and ATP-Competitive Inhibitors of mTOR Effectively Suppress Tumor Progression-Associated Epithelial-Mesenchymal Transition in the Kidneys of Tsc2+/− Mice
    Article Snippet: For MS-IHC, previous IHC-stained slides were incubated in xylene for 10 minutes to remove coverslips and then incubated at 50°C in a buffered solution containing 5% SDS, 0.5% mercaptoethanol, and 50 mm Tris–HCl (pH 7.5) for 60 minutes to strip primary antibodies, and finally, the protocol was followed for conventional IHC. .. SignalStain Boost Rabbit specific IHC Detection Reagent (Cell Signaling Technology, Danvers, MA) and ImmPACT NovaRED Peroxidase Substrate or ImmPACT VIP Peroxidase (HRP) Substrate (Vector Laboratories, Peterborough, UK) were used to stain antigens according to the kit suppliers' instruction. .. IHC or MS-IHC stained slides were scanned to generate virtual slides for photo capture using an Aperio system.

    TUNEL Assay:

    Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis
    Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the ImmPACT VIP Peroxidase Substrate kit (Vector Laboratories). .. This kit produces a purple product that is easily distinguished from the brown product of the TUNEL staining.

    Marker:

    Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis
    Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the ImmPACT VIP Peroxidase Substrate kit (Vector Laboratories). .. This kit produces a purple product that is easily distinguished from the brown product of the TUNEL staining.

    Activity Assay:

    Article Title: An Inhibitor of Arginine‐Glycine‐Aspartate‐Binding Integrins Reverses Fibrosis in a Mouse Model of Nonalcoholic Steatohepatitis
    Article Snippet: .. To detect apoptotic cells with HSC morphology, TUNEL‐stained liver tissues were subsequently stained with the antibody to desmin (a marker of HSC) as described in Materials and Methods, except that secondary antibody peroxidase activity was detected with the ImmPACT VIP Peroxidase Substrate kit (Vector Laboratories). .. This kit produces a purple product that is easily distinguished from the brown product of the TUNEL staining.

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  • 93
    Vector Laboratories immpact vip peroxidase hrp substrate
    Assay validation a , Different chromogenic substrates were tested to reveal Ki67 in lymphoma cell lines. Among those tested, the <t>ImmPACT</t> <t>VIP</t> substrate showed the greatest contrast between stained and unstained control samples. b , The CEM measured marker expressions of kappa light chain, lambda light chain and Ki67 for three different cell lines (Daudi, DB and Jurkat) and compared to marker expressions measured by gold-standard flow cytometry. Note the congruency. c , Ki67 Antibodies were lyophilized and stored at different temperatures. After rehydration, the antibodies and the CEM assay showed good reproducibility with a standard variation of
    Immpact Vip Peroxidase Hrp Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpact vip peroxidase hrp substrate/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpact vip peroxidase hrp substrate - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    Assay validation a , Different chromogenic substrates were tested to reveal Ki67 in lymphoma cell lines. Among those tested, the ImmPACT VIP substrate showed the greatest contrast between stained and unstained control samples. b , The CEM measured marker expressions of kappa light chain, lambda light chain and Ki67 for three different cell lines (Daudi, DB and Jurkat) and compared to marker expressions measured by gold-standard flow cytometry. Note the congruency. c , Ki67 Antibodies were lyophilized and stored at different temperatures. After rehydration, the antibodies and the CEM assay showed good reproducibility with a standard variation of

    Journal: Nature biomedical engineering

    Article Title: Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning

    doi: 10.1038/s41551-018-0265-3

    Figure Lengend Snippet: Assay validation a , Different chromogenic substrates were tested to reveal Ki67 in lymphoma cell lines. Among those tested, the ImmPACT VIP substrate showed the greatest contrast between stained and unstained control samples. b , The CEM measured marker expressions of kappa light chain, lambda light chain and Ki67 for three different cell lines (Daudi, DB and Jurkat) and compared to marker expressions measured by gold-standard flow cytometry. Note the congruency. c , Ki67 Antibodies were lyophilized and stored at different temperatures. After rehydration, the antibodies and the CEM assay showed good reproducibility with a standard variation of

    Article Snippet: Captured cells were stained with Immpact VIP Peroxidase (HRP) substrate (Vector Labs) for 15 min. Three images per sample (pre-wash, post-wash and stained) were taken using the CEM device.

    Techniques: Staining, Marker, Flow Cytometry, Cytometry