peroxidase  (Vector Laboratories)


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    Name:
    VECTOR VIP Peroxidase HRP Substrate Kit
    Description:
    Vector VIP Peroxidase Substrate Kit produces an intense violet purple reaction products and can be used as alternatives to DAB or as a second color for multiple antigen labeling Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina 1 Features Greater sensitivity than conventional substratesConsistent and reliableIdeal for IHC ICC ISH and blotsPermanent mountingIdeal for single and multiple labelingStock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tabletsOne year expiry dateSufficient reagents to produce 300 ml of working solution With the aid of imaging systems and software the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co localized Sections stained with either substrate can be viewed by darkfield and electron microscopy Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods Stained sections should be dehydrated cleared and permanently mounted Vector VIP Substrate Kit contains stock solutions in convenient dropper bottles The sensitivity of Vector VIP substrate is equivalent to Vector DAB Substrate This product can also be used on blots The kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4° C this kit is stable for one year
    Catalog Number:
    sk-4600
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories peroxidase
    VECTOR VIP Peroxidase HRP Substrate Kit
    Vector VIP Peroxidase Substrate Kit produces an intense violet purple reaction products and can be used as alternatives to DAB or as a second color for multiple antigen labeling Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina 1 Features Greater sensitivity than conventional substratesConsistent and reliableIdeal for IHC ICC ISH and blotsPermanent mountingIdeal for single and multiple labelingStock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tabletsOne year expiry dateSufficient reagents to produce 300 ml of working solution With the aid of imaging systems and software the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co localized Sections stained with either substrate can be viewed by darkfield and electron microscopy Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods Stained sections should be dehydrated cleared and permanently mounted Vector VIP Substrate Kit contains stock solutions in convenient dropper bottles The sensitivity of Vector VIP substrate is equivalent to Vector DAB Substrate This product can also be used on blots The kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4° C this kit is stable for one year
    https://www.bioz.com/result/peroxidase/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase - by Bioz Stars, 2021-03
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination
    Article Snippet: Dako wash buffer (Dako; Agilent Technologies, Inc.) was used for all washing steps. .. To detect mouse TIMP-1, sections were blocked with 5% donkey serum (cat. no. 0030-01; Southern Biotech, Birmingham, AL, USA) for 1 h at room temperature, in order to block non-specific protein binding, and were immunolabeled overnight at 4°C with goat anti-mouse TIMP-1 antibody (1:50 dilution), followed by 1 h incubation at room temperature with a 1:250 dilution of biotinylated donkey anti-goat antibody, which was visualized using the Vectastain ABC reagent (30 min at room temperature; reagent was diluted by mixing 100 µ l of each reagent A and B in 5 ml PBS) and VIP peroxidase substrate kit (Vector Laboratories, Inc.). .. For detection of PCNA, sections were immunolabeled overnight at 4°C with PCNA antibody (1:200) in 2% BSA-PBS, followed by biotinylated goat anti rabbit antibody (1:500) for 1 h at room temperature and ABC reagent for 30 min at room temperature.

    Article Title: Therapeutic antibody targeting microtubule-binding domain prevents neuronal internalization of extracellular tau via masking neuron surface proteoglycans
    Article Snippet: .. The sections were blocked with Aptum section block (Aptum, Southampton, UK) followed by incubation with mouse monoclonal primary antibody (Anti -human phospho tau AT8, 1:1000, ThermoScientific, IL, USA) overnight at 4 °C and were immunostained using the standard avidin-biotin-peroxidase method (Vectastain ABC kit) with VIP as chromogen (VIP kit, Vector Laboratories, Burlingame, CA, USA). .. After mounting, sections were evaluated using Olympus BX51 microscope.

    Protein Binding:

    Article Title: Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination
    Article Snippet: Dako wash buffer (Dako; Agilent Technologies, Inc.) was used for all washing steps. .. To detect mouse TIMP-1, sections were blocked with 5% donkey serum (cat. no. 0030-01; Southern Biotech, Birmingham, AL, USA) for 1 h at room temperature, in order to block non-specific protein binding, and were immunolabeled overnight at 4°C with goat anti-mouse TIMP-1 antibody (1:50 dilution), followed by 1 h incubation at room temperature with a 1:250 dilution of biotinylated donkey anti-goat antibody, which was visualized using the Vectastain ABC reagent (30 min at room temperature; reagent was diluted by mixing 100 µ l of each reagent A and B in 5 ml PBS) and VIP peroxidase substrate kit (Vector Laboratories, Inc.). .. For detection of PCNA, sections were immunolabeled overnight at 4°C with PCNA antibody (1:200) in 2% BSA-PBS, followed by biotinylated goat anti rabbit antibody (1:500) for 1 h at room temperature and ABC reagent for 30 min at room temperature.

    Immunolabeling:

    Article Title: Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination
    Article Snippet: Dako wash buffer (Dako; Agilent Technologies, Inc.) was used for all washing steps. .. To detect mouse TIMP-1, sections were blocked with 5% donkey serum (cat. no. 0030-01; Southern Biotech, Birmingham, AL, USA) for 1 h at room temperature, in order to block non-specific protein binding, and were immunolabeled overnight at 4°C with goat anti-mouse TIMP-1 antibody (1:50 dilution), followed by 1 h incubation at room temperature with a 1:250 dilution of biotinylated donkey anti-goat antibody, which was visualized using the Vectastain ABC reagent (30 min at room temperature; reagent was diluted by mixing 100 µ l of each reagent A and B in 5 ml PBS) and VIP peroxidase substrate kit (Vector Laboratories, Inc.). .. For detection of PCNA, sections were immunolabeled overnight at 4°C with PCNA antibody (1:200) in 2% BSA-PBS, followed by biotinylated goat anti rabbit antibody (1:500) for 1 h at room temperature and ABC reagent for 30 min at room temperature.

    Incubation:

    Article Title: Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination
    Article Snippet: Dako wash buffer (Dako; Agilent Technologies, Inc.) was used for all washing steps. .. To detect mouse TIMP-1, sections were blocked with 5% donkey serum (cat. no. 0030-01; Southern Biotech, Birmingham, AL, USA) for 1 h at room temperature, in order to block non-specific protein binding, and were immunolabeled overnight at 4°C with goat anti-mouse TIMP-1 antibody (1:50 dilution), followed by 1 h incubation at room temperature with a 1:250 dilution of biotinylated donkey anti-goat antibody, which was visualized using the Vectastain ABC reagent (30 min at room temperature; reagent was diluted by mixing 100 µ l of each reagent A and B in 5 ml PBS) and VIP peroxidase substrate kit (Vector Laboratories, Inc.). .. For detection of PCNA, sections were immunolabeled overnight at 4°C with PCNA antibody (1:200) in 2% BSA-PBS, followed by biotinylated goat anti rabbit antibody (1:500) for 1 h at room temperature and ABC reagent for 30 min at room temperature.

    Article Title: Therapeutic antibody targeting microtubule-binding domain prevents neuronal internalization of extracellular tau via masking neuron surface proteoglycans
    Article Snippet: .. The sections were blocked with Aptum section block (Aptum, Southampton, UK) followed by incubation with mouse monoclonal primary antibody (Anti -human phospho tau AT8, 1:1000, ThermoScientific, IL, USA) overnight at 4 °C and were immunostained using the standard avidin-biotin-peroxidase method (Vectastain ABC kit) with VIP as chromogen (VIP kit, Vector Laboratories, Burlingame, CA, USA). .. After mounting, sections were evaluated using Olympus BX51 microscope.

    Article Title: ETHANOL CAUSES AND LITHIUM PREVENTS NEUROAPOPTOSIS AND SUPPRESSION OF pERK IN THE INFANT MOUSE BRAIN
    Article Snippet: The sections were then incubated for 1 hr in secondary antibody (goat anti-rabbit 1:200 in blocking solution), and reacted in the dark with ABC reagents (standard Vectastain ABC Elite Kit, Vector Labs, Burlingame, CA) for 1 hour. .. The sections were then washed 3 times with PBS, and incubated with VIP reagent (Vector VIP substrate kit for peroxidase, Vector Labs, Burlingame, CA) to develop a purple color. .. Quantitative counts were performed on serial vibratome sections (70µm) stained by the AC3 method and chosen using systematic random sampling in accordance with principles of unbiased stereology ( ).

    Avidin-Biotin Assay:

    Article Title: Differential DNA damage signaling accounts for distinct neural apoptotic responses in ATLD and NBS
    Article Snippet: Immunohistochemistry for activated caspase-3 or p53-ser15 was performed overnight at room temperature after quenching endogenous peroxidase with 0.6% hydrogen peroxide in methanol. .. Immunoreactivity was visualized with a VIP substrate kit (Vector Laboratories) according to the manufacturer's directions after tissues were treated with biotinylated secondary antibody and avidin DH-biotinylated horseradish peroxidase-H complex (Vectastain Elite kit; Vector Laboratories). .. Sections were counterstained with 0.1% methyl green (Vector Laboratories), dehydrated, and mounted in DPX reagent.

    Article Title: Therapeutic antibody targeting microtubule-binding domain prevents neuronal internalization of extracellular tau via masking neuron surface proteoglycans
    Article Snippet: .. The sections were blocked with Aptum section block (Aptum, Southampton, UK) followed by incubation with mouse monoclonal primary antibody (Anti -human phospho tau AT8, 1:1000, ThermoScientific, IL, USA) overnight at 4 °C and were immunostained using the standard avidin-biotin-peroxidase method (Vectastain ABC kit) with VIP as chromogen (VIP kit, Vector Laboratories, Burlingame, CA, USA). .. After mounting, sections were evaluated using Olympus BX51 microscope.

    Plasmid Preparation:

    Article Title: ETHANOL CAUSES AND LITHIUM PREVENTS NEUROAPOPTOSIS AND SUPPRESSION OF pERK IN THE INFANT MOUSE BRAIN
    Article Snippet: The sections were then incubated for 1 hr in secondary antibody (goat anti-rabbit 1:200 in blocking solution), and reacted in the dark with ABC reagents (standard Vectastain ABC Elite Kit, Vector Labs, Burlingame, CA) for 1 hour. .. The sections were then washed 3 times with PBS, and incubated with VIP reagent (Vector VIP substrate kit for peroxidase, Vector Labs, Burlingame, CA) to develop a purple color. .. Quantitative counts were performed on serial vibratome sections (70µm) stained by the AC3 method and chosen using systematic random sampling in accordance with principles of unbiased stereology ( ).

    Article Title: Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches
    Article Snippet: .. Immunohistochemical staining was performed overnight at +4°C with affinity-purified anti-PIR antibody, followed by detection with VECTOR VIP (SK-4600, Peroxidase substrate kit, Vector laboratories, Inc. Burlingame, Ca 94010). .. For rabbit polyclonal anti-PIR antibody production, EcoRI-SmaI digested PIR full length coding sequence was inserted downstream to the GST sequence in pGEX-4T1 vector (Pharmacia Biotech) and the construct was transformed in BL21 strain of E. coli to express GST-PIR fusion protein.

    Article Title: The Role of the Mouse Y Chromosome on Susceptibility to Testicular Germ Cell Tumors
    Article Snippet: .. Substrate development was performed with the Vector VIP Substrate kit (Vector Laboratories). .. Developed slides were dehydrated in an ascending ethanol series, cleared in xylene, and mounted with VectaMount permanent mounting medium (Vector Laboratories).

    Immunohistochemistry:

    Article Title: Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches
    Article Snippet: .. Immunohistochemical staining was performed overnight at +4°C with affinity-purified anti-PIR antibody, followed by detection with VECTOR VIP (SK-4600, Peroxidase substrate kit, Vector laboratories, Inc. Burlingame, Ca 94010). .. For rabbit polyclonal anti-PIR antibody production, EcoRI-SmaI digested PIR full length coding sequence was inserted downstream to the GST sequence in pGEX-4T1 vector (Pharmacia Biotech) and the construct was transformed in BL21 strain of E. coli to express GST-PIR fusion protein.

    Staining:

    Article Title: Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches
    Article Snippet: .. Immunohistochemical staining was performed overnight at +4°C with affinity-purified anti-PIR antibody, followed by detection with VECTOR VIP (SK-4600, Peroxidase substrate kit, Vector laboratories, Inc. Burlingame, Ca 94010). .. For rabbit polyclonal anti-PIR antibody production, EcoRI-SmaI digested PIR full length coding sequence was inserted downstream to the GST sequence in pGEX-4T1 vector (Pharmacia Biotech) and the construct was transformed in BL21 strain of E. coli to express GST-PIR fusion protein.

    Affinity Purification:

    Article Title: Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches
    Article Snippet: .. Immunohistochemical staining was performed overnight at +4°C with affinity-purified anti-PIR antibody, followed by detection with VECTOR VIP (SK-4600, Peroxidase substrate kit, Vector laboratories, Inc. Burlingame, Ca 94010). .. For rabbit polyclonal anti-PIR antibody production, EcoRI-SmaI digested PIR full length coding sequence was inserted downstream to the GST sequence in pGEX-4T1 vector (Pharmacia Biotech) and the construct was transformed in BL21 strain of E. coli to express GST-PIR fusion protein.

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  • 99
    Vector Laboratories vip peroxidase substrate kit
    Vip Peroxidase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vip peroxidase substrate kit/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vip peroxidase substrate kit - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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