immpact amec red substrate  (Vector Laboratories)


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    Name:
    ImmPACT AMEC Red Peroxidase HRP Substrate
    Description:
    ImmPACT AMEC Red Peroxidase Substrate is 5 10 times greater sensitivity than conventional AEC substratesOptimized for IHC ICC ISH and blotsAqueous mountingSuitable for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated stock solutions in dropper bottles ImmPACT AMEC Red HRP Substrate utilizes Vector Laboratories ImmPACT technology to generate a crisper brighter red reaction product that is 5 10 times more sensitive than conventional AEC substrates and can be used as a single label or as a second color for multiple antigen labeling ImmPACT AMEC Red chromogen can be used for both manual and automated staining methods Stained slides must be aqueously mounted because the reaction product is soluble in organic solvents Precipitated ImmPACT AMEC Red is stable for at least 2 years when mounted in VectaMount AQ H 5501 ImmPACT AMEC Red consists of 120 ml of diluent and concentrated stock solutions of ImmPACT reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4° C
    Catalog Number:
    sk-4285
    Price:
    None
    Size:
    120 ml
    Category:
    Protein chromogenic detection reagents or kits or substrates
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    Structured Review

    Vector Laboratories immpact amec red substrate
    ImmPACT AMEC Red Peroxidase HRP Substrate
    ImmPACT AMEC Red Peroxidase Substrate is 5 10 times greater sensitivity than conventional AEC substratesOptimized for IHC ICC ISH and blotsAqueous mountingSuitable for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated stock solutions in dropper bottles ImmPACT AMEC Red HRP Substrate utilizes Vector Laboratories ImmPACT technology to generate a crisper brighter red reaction product that is 5 10 times more sensitive than conventional AEC substrates and can be used as a single label or as a second color for multiple antigen labeling ImmPACT AMEC Red chromogen can be used for both manual and automated staining methods Stained slides must be aqueously mounted because the reaction product is soluble in organic solvents Precipitated ImmPACT AMEC Red is stable for at least 2 years when mounted in VectaMount AQ H 5501 ImmPACT AMEC Red consists of 120 ml of diluent and concentrated stock solutions of ImmPACT reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 4° C
    https://www.bioz.com/result/immpact amec red substrate/product/Vector Laboratories
    Average 93 stars, based on 1335 article reviews
    Price from $9.99 to $1999.99
    immpact amec red substrate - by Bioz Stars, 2021-02
    93/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Nuclear Progestin Receptor Phosphorylation by Cdk9 Is Required for the Expression of Mmp15, a Protease Indispensable for Ovulation in Medaka
    Article Snippet: .. Immunohistochemistry Immunohistochemistry was performed according to the method previously described in [ ], except that the signal was detected using an ImmPACT AMEC Red Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA, USA), according to the manufacturer’s instructions. .. Knockout Experiments for cdk9 The Cas9 nuclease expression vector was generated as follows.

    Avidin-Biotin Assay:

    Article Title: Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering
    Article Snippet: .. Secondary antibodies included rabbit–anti rat biotin conjugated (BA-4001) followed by ImmPACT AMEC Red Substrate (Vector Laboratories, Burlingame, CA, USA), rabbit–anti rat TRITC (PA1-28570, Thermofisher Scientific, Waltham, MA, USA), rabbit–anti rat AF647 (ab169349, Abcam, Cambridge, UK), goat–anti-rabbit (BA-1000) followed by fluorescein avidin DCS (A-2011, Vector Laboratories, Burlingame, CA, USA), donkey-anti-sheep TRITC (ab6897, Abcam, Cambridge, UK), alpaca-anti-rabbit AF488 (ChromoTek, Planegg-Martinsried, Germany), and DAPI Mounting Medium (Carl Roth, Karlsruhe, Germany) according to the manufacturers’ instructions. .. Images were recorded with the Axioplan 2 and Apotome 2 imaging light-/fluorescence microscope with an AxioCam camera (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) and a confocal Leica TCS SP8 X microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Nanoparticle-Aided Characterization of Arterial Endothelial Architecture during Atherosclerosis Progression and Metabolic Therapy
    Article Snippet: .. Thereafter, avidin-peroxidase (Vectastatin Elite ABC HRP Kit, Vector Laboratories) was added for another 30 min. Color development was induced using ImmPact AMEC red peroxidase substrate (Vector Laboratories). .. Images were acquired using a light microscope (Leica Microsystems) at 100× magnification.

    other:

    Article Title: Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells
    Article Snippet: In conjunction with anti-LAMP1 antibody (Cell Signaling Technologies) Ultratech HRP 500–600 tests (catalogue # IM2391, Beckman-Coulter) and ImmPACT AMEC Red Peroxidase HRP substrate (catalogue # SK-4285, Vector Laboratories) were used to visualize LAMP1.

    Article Title: The transmembrane protein LRIG2 increases tumor progression in skin carcinogenesis
    Article Snippet: ImmPACT® AMEC Red or DAB Peroxidase (HRP) substrate (Vector Laboratories) were used as chromogen.

    Activity Assay:

    Article Title: Reducing GBA2 Activity Ameliorates Neuropathology in Niemann-Pick Type C Mice
    Article Snippet: .. HRP activity was detected using ImmPACT AMEC Red substrate (SK-4285; Vector Laboratories). .. Sections were counterstained with methyl green and mounted with VectaMount (Vector Laboratories).

    Staining:

    Article Title: Glucose lowering by SGLT2-inhibitor empagliflozin accelerates atherosclerosis regression in hyperglycemic STZ-diabetic mice
    Article Snippet: .. After washing with PBS for 5 min at RT, staining was developed with ImmPACT AMEC Red Substrate (Vector Laboratories, Burlingame, CA, USA, #SK-4285) for 1 to 3 min. Stained sections were washed for 20 min with rinsing water and cell nuclei were counterstained with hematoxylin. .. Ki67/CD68/DAPI immunofluorescence stain After fixation (PFA4% for 10 min) and permeabilization (Triton X-100 0.5% (No: A4975,0100, PanReac AppliChem, Barcelona, Spain) with Tween20 0.25% (No: 142312.1611, PanReac AppliChem, Barcelona, Spain) in PBS for 5 min) slides were incubated in goat serum 10% (Normal Goat Serum Blocking Solution, Cat: S-1000, Vector Laboratories, Peterborough, GB) for 1 hour.

    Binding Assay:

    Article Title: O-GlcNAcylation enhances double strand break repair, promotes cancer cell proliferation and prevents therapy-induced senescence in irradiated tumors
    Article Snippet: .. Antigen-antibody binding was detected using the ImmPACT AMEC Red peroxidase substrate kit (Vector Laboratories, SK-4285) for Ki-67 or ImmPACT DAB peroxidase (Vector Laboratories, SK-4105) for EZH2. .. Ki-67 and EZH2 stained tissue sections were briefly immersed in hematoxylin for counterstaining, washed 3X in dH2O, mounted using Aqua-Poly/Mount and covered with cover glasses.

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  • 93
    Vector Laboratories immpact amec red substrate
    Immpact Amec Red Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpact amec red substrate/product/Vector Laboratories
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    immpact amec red substrate - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

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