chromogen  (Vector Laboratories)


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    Name:
    ImmPACT AEC Peroxidase HRP Substrate
    Description:
    ImmPACT AEC Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kit Optimized for IHC ICC ISH and blots Aqueous mounting Suitable for single and multiple labeling Working solution stable for up to 14 days at 4C One year expiry date Supplied as 120 ml of diluent and stock solutions in dropper bottles AEC 3 amino 9 ethylcarbazole and ImmPACT AEC HRP Substrates both produce red reaction products that can be used as a single label or as a second color for multiple antigen labeling AEC and ImmPACT AEC chromogens can be used for both manual and automated staining methods Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents Sections stained with AEC are stable for at least 4 years when mounted in VectaMount AQ H 5501 This product utilizes Vector Laboratories novel ImmPACT technology to generate a crisper brighter red reaction product that is 3 5 times more sensitive than conventional AEC substrates such as AEC Substrate Kit SK 4200 This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT AEC reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 47° C
    Catalog Number:
    sk-4205
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Size:
    120 ml
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    Structured Review

    Vector Laboratories chromogen
    ImmPACT AEC Peroxidase HRP Substrate
    ImmPACT AEC Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kit Optimized for IHC ICC ISH and blots Aqueous mounting Suitable for single and multiple labeling Working solution stable for up to 14 days at 4C One year expiry date Supplied as 120 ml of diluent and stock solutions in dropper bottles AEC 3 amino 9 ethylcarbazole and ImmPACT AEC HRP Substrates both produce red reaction products that can be used as a single label or as a second color for multiple antigen labeling AEC and ImmPACT AEC chromogens can be used for both manual and automated staining methods Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents Sections stained with AEC are stable for at least 4 years when mounted in VectaMount AQ H 5501 This product utilizes Vector Laboratories novel ImmPACT technology to generate a crisper brighter red reaction product that is 3 5 times more sensitive than conventional AEC substrates such as AEC Substrate Kit SK 4200 This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT AEC reagents in convenient dropper bottles This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes Working solutions are stable for up to 14 days at 47° C
    https://www.bioz.com/result/chromogen/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chromogen - by Bioz Stars, 2021-03
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Haploinsufficiency of Transferrin Receptor 1 Impairs Angiogenesis with Reduced Mitochondrial Complex I in Mice with Limb Ischemia
    Article Snippet: Primary antibody was sequentially coupled with biotin-conjugated goat anti-rat antibody (#BA-4000, Vector Laboratories, CA, USA) and visualized by VECTASTAIN Elite ABC HRP kit (#PK6100, Vector Laboratories). .. The staining was then revealed using a 3-amino-9-ethylcarbazole (AEC) peroxidase substrate kit (#SK4205, Vector Laboratories). ..

    Article Title: LETM1 is a potential biomarker of prognosis in lung non-small cell carcinoma
    Article Snippet: Primarily, for the LETM1 protocols, except that the chromogen with the 3, 3′-diaminobenzidine (Dako) for 10 min (FLEX20), all steps are the same. .. Then, subsequent staining of the same section was performed after incubating the samples with an antibody to CD105 by ImmPACT AEC Peroxidase Substrate for 20 min. .. NSCLC cell lines Three human NSCLC cell lines A549, H1299 and H1650 were purchased from ATCC (Manassas, USA) and maintained in DMEM with high glucose (Life Technologies, Grand Island, NY) containing 10% fetal bovine serum (Life Technologies).

    Plasmid Preparation:

    Article Title: The Herpesviridae Conserved Multifunctional Infected-Cell Protein 27 (ICP27) Is Important but Not Required for Replication and Oncogenicity of Marek’s Disease Alphaherpesvirus
    Article Snippet: Sections were cut at 8 μm and stained with hematoxylin and eosin (H & E) or rabbit polyclonal anti-Meq antibody plus hematoxylin as a counterstain. .. The Vectastain Universal Elite ABC, ImmPACT AEC HRP substrate and Vector hematoxylin QS kits were used (Vector Laboratories, Burlingame, CA). ..

    Incubation:

    Article Title: A Brain Penetrating Bifunctional Erythropoietin-Transferrin Receptor Antibody Fusion Protein for Alzheimer’s Disease
    Article Snippet: After washing with PBS, sections were incubated for 60 min with PBS containing 0.5% BSA and 0.3% Triton-X100 to block non-specific protein binding and incubated overnight at 4°C with a biotin-conjugated rabbit anti-Iba1 (1:200 dilution, Wako Chemicals USA, Richmond, VA). .. Sections were then incubated for 1h at RT with ABC complex according to manufacturer instructions (Vector Laboratories, Burlingame, CA) and developed using the ImmPACT AEC Peroxidase (HRP) Substrate kit as per the vendors instructions (Vector Labs, Burlingame, CA). .. After washing, sections were mounted on slides using the Vectamount aqueous mounting media (Vector labs, Burlingame, CA), coverslipped, and sealed with clear nail polish.

    Article Title: The ameliorative effect of monotropein, astragalin, and spiraeoside on oxidative stress, endoplasmic reticulum stress, and mitochondrial signaling pathway in varicocelized rats
    Article Snippet: Tissue sections were incubated with an anti-Grp78 and StAR protein antibody (rabbit monoclonal, 1: 100; Abcam Cambridge, MA USA, Anti-Grp78, catalog number: ab21685; Cell Signaling, Beverly, MA, USA; StAR, catalog number: D10H12;) for 24 h at 4 °C after blocking with serum block solution for 10 min at room temperature (DAKO, X0909). .. The slides were further rinsed in 1 X PBS and incubated with a secondary antibody (Anti-rabbit IgG; vector Labs, Burlingame, CA, USA; catalog number: MP-7451) for 1 h followed by AEC substrate chromogens (ImmPACT AEC Peroxidase substrate; vector Labs, Burlingame, CA, USA; catalog number: SK-4205) and washed with deionized water, and counter-stained with hematoxylin. .. The slides were washed with tap water and finally mounted with an aqueous medium (Abcam Cambridge, MA USA).

    Amplification:

    Article Title: Gut microbiota profiling in Norwegian weaner pigs reveals potentially beneficial effects of a high-fiber rapeseed diet
    Article Snippet: Reagents from the Vectastaom Elite-HRP kit (Vector Laboratories, Cat.no. .. PK-6100) was applied for signal amplification, and immunoreactivity was detected using ImmPact AEC peroxidase substrate (Vector Laboratories, Cat.no. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Gut microbiota profiling in Norwegian weaner pigs reveals potentially beneficial effects of a high-fiber rapeseed diet
    Article Snippet: Reagents from the Vectastaom Elite-HRP kit (Vector Laboratories, Cat.no. .. PK-6100) was applied for signal amplification, and immunoreactivity was detected using ImmPact AEC peroxidase substrate (Vector Laboratories, Cat.no. ..

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  • 98
    Vector Laboratories immpact aec peroxidase substrate
    Immpact Aec Peroxidase Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpact aec peroxidase substrate/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpact aec peroxidase substrate - by Bioz Stars, 2021-03
    98/100 stars
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