aec  (Vector Laboratories)


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    Name:
    AEC Peroxidase HRP Substrate Kit 3 amino 9 ethylcarbazole
    Description:
    AEC Peroxidase Substrate Kit has greater sensitivity than conventional substrates Consistent and reliable Suitable for IHC ICC and blots Aqueous mountingSuitable for single and multiple labelingStock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets One year expiry date Sufficient reagents to produce 300 ml of working solution AEC 3 amino 9 ethylcarbazole and ImmPACT AEC HRP substrates both produce red reaction products that can be used as a single label or as a second color for multiple antigen labeling AEC and ImmPACT AEC chromogens can be used for both manual and automated staining methods Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents Sections stained with AEC are stable for at least 4 years when mounted in VectaMount AQ H 5501 This kit contains stock solutions in convenient dropper bottles This product can also be used on blots The kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4 C this kit is stable for one year
    Catalog Number:
    sk-4200
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories aec
    AEC Peroxidase HRP Substrate Kit 3 amino 9 ethylcarbazole
    AEC Peroxidase Substrate Kit has greater sensitivity than conventional substrates Consistent and reliable Suitable for IHC ICC and blots Aqueous mountingSuitable for single and multiple labelingStock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets One year expiry date Sufficient reagents to produce 300 ml of working solution AEC 3 amino 9 ethylcarbazole and ImmPACT AEC HRP substrates both produce red reaction products that can be used as a single label or as a second color for multiple antigen labeling AEC and ImmPACT AEC chromogens can be used for both manual and automated staining methods Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents Sections stained with AEC are stable for at least 4 years when mounted in VectaMount AQ H 5501 This kit contains stock solutions in convenient dropper bottles This product can also be used on blots The kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4 C this kit is stable for one year
    https://www.bioz.com/result/aec/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aec - by Bioz Stars, 2021-03
    96/100 stars

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    Incubation:

    Article Title: Treatment of murine lupus with cDNA encoding IFN-?R/Fc
    Article Snippet: When required, sections were sequentially incubated with biotinylated secondary antibodies (Jackson ImmunoResearch). .. In these cases, sections were incubated with streptavidin horseradish peroxidase (Vector Labs), developed with a peroxidase substrate AEC kit (Vector Labs), and counterstained with Mayer’s hematoxylin. ..

    Staining:

    Article Title: Tissue-specific expression of the human prostate-specific antigen gene in transgenic mice: Implications for tolerance and immunotherapy
    Article Snippet: Prostate and coagulating gland/seminal vesicle tissue removed from nontransgenic or transgenic mice were fixed in formalin, embedded with paraffin, and sections (5 μm) were placed onto poly- l -lysine-coated slides. .. Following quenching of endogenous peroxidase, the sections were blocked with normal goat serum, stained with a 1:200 dilution of rabbit anti-human PSA (Dako) followed by goat anti-rabbit Ig conjugated to horse radish peroxidase (Dako), and visualized by adding metal-enhanced diaminobenzidine (Pierce) or 3-amino-9-ethylcarbazole (Vector Laboratories) as the substrate. .. TIL were purified from tumors grown in transgenic or nontransgenic mice 20 days after injection of 2 × 104 line 1/PSA cells using paramagnetic beads (Dynal, Great Neck, NY) conjugated with anti-Thy-1 monoclonal antibody, as described previously ( ).

    Article Title: Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways
    Article Snippet: .. For visualization of the specific staining, Vectastain ABC reagent and 3-amino-9-ethylcarbazole (AEC) substrate (all purchased from Vector Laboratories, Burlingame, CA, USA) were applied. ..

    Article Title: Expression and Function of Chemokine Receptors on Human Thymocytes: Implications for Infection by Human Immunodeficiency Virus Type 1
    Article Snippet: Sections were treated with an avidin-biotin blocking kit (Vector Labs, Burlingame, Calif.) and then incubated with isotype control MAb or with anti-CCR5 or anti-CXCR4 MAb. .. The staining was developed with a Vectastain Elite ABC kit with 3-amino-9-ethylcarbazole substrate (Vector Labs). .. Sections were counterstained with hematoxylin and mounted with Crystal/Mount (both from Biomeda Corp., Foster City, Calif.).

    Immunohistochemistry:

    Article Title: Immunophenotyping of Rabbit Testicular Germ and Sertoli Cells Across Maturational Stages
    Article Snippet: After washing in Tris buffer, the sections were covered with antimouse IgG biotinylated antibody (diluted 1:200) for monoclonal antibodies or with antigoat IgG biotinylated antibody (diluted 1:200) for AMH and incubated at room temperature for 30 min. After washing, the peroxidase-conjugate ABC (diluted 1:100) was allowed to react at room temperature for 30 min. .. The immunohistochemical reaction was developed with 3-amino-9-ethylcarbazole (Vector Laboratories) for 10 min according to the manufacturer’s instructions. ..

    Article Title: In Situ Visualization of Intratumor Growth Factor Signaling
    Article Snippet: Immunohistochemistry was performed using the avidin-biotin-peroxidase complex (ABC) method according to the manufacturer’s instructions (Vectastain Elite Kit, Vector Laboratories, Burlingame, CA) using diaminobenzidine as the chromogen. .. Double-label immunohistochemistry was performed sequentially using the peroxidase substrates aminoethylcarbazole and Vector SG, according to the manufacturer’s instructions (Vector Laboratories, Burlingame, CA). .. Digital images were acquired with a Dage DC-330 three-color CCD camera controlled by Image Pro-Plus software.

    Plasmid Preparation:

    Article Title: In Situ Visualization of Intratumor Growth Factor Signaling
    Article Snippet: Immunohistochemistry was performed using the avidin-biotin-peroxidase complex (ABC) method according to the manufacturer’s instructions (Vectastain Elite Kit, Vector Laboratories, Burlingame, CA) using diaminobenzidine as the chromogen. .. Double-label immunohistochemistry was performed sequentially using the peroxidase substrates aminoethylcarbazole and Vector SG, according to the manufacturer’s instructions (Vector Laboratories, Burlingame, CA). .. Digital images were acquired with a Dage DC-330 three-color CCD camera controlled by Image Pro-Plus software.

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    Vector Laboratories 3 amino 9 ethylcarbazole aec substrate
    Immunocytochemical detection of PRL receptors in cumulus cells after 20 h maturation of bovine cumulus-enclosed oocytes. Specific localizations were detected by MA1-610 antibody and the red <t>3-amino-9-ethylcarbazole</t> (AEC) chromophore. Nuclei were counterstained with hematoxylin. (A) Positive staining. Black arrows indicate PRL receptor-specific immunoreaction. (B) Negative control performed by omitting the primary antibody. Original magnification: × 400.
    3 Amino 9 Ethylcarbazole Aec Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 amino 9 ethylcarbazole aec substrate/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 amino 9 ethylcarbazole aec substrate - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

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    Immunocytochemical detection of PRL receptors in cumulus cells after 20 h maturation of bovine cumulus-enclosed oocytes. Specific localizations were detected by MA1-610 antibody and the red 3-amino-9-ethylcarbazole (AEC) chromophore. Nuclei were counterstained with hematoxylin. (A) Positive staining. Black arrows indicate PRL receptor-specific immunoreaction. (B) Negative control performed by omitting the primary antibody. Original magnification: × 400.

    Journal: Frontiers in Genetics

    Article Title: Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

    doi: 10.3389/fgene.2015.00274

    Figure Lengend Snippet: Immunocytochemical detection of PRL receptors in cumulus cells after 20 h maturation of bovine cumulus-enclosed oocytes. Specific localizations were detected by MA1-610 antibody and the red 3-amino-9-ethylcarbazole (AEC) chromophore. Nuclei were counterstained with hematoxylin. (A) Positive staining. Black arrows indicate PRL receptor-specific immunoreaction. (B) Negative control performed by omitting the primary antibody. Original magnification: × 400.

    Article Snippet: For visualization of the specific staining, Vectastain ABC reagent and 3-amino-9-ethylcarbazole (AEC) substrate (all purchased from Vector Laboratories, Burlingame, CA, USA) were applied.

    Techniques: Staining, Negative Control

    Immunocytochemical detection of GH receptors in cumulus cells after 20 h maturation of bovine cumulus-enclosed oocytes. Specific localizations were detected by MAB 263 antibody and the red 3-amino-9-ethylcarbazole (AEC) chromophore. Nuclei were counterstained with hematoxylin. (A) Positive staining. Black arrows indicate GH receptor-specific immunoreaction. (B) Negative control performed by omitting the primary antibody. Original magnification: × 400.

    Journal: Frontiers in Genetics

    Article Title: Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

    doi: 10.3389/fgene.2015.00274

    Figure Lengend Snippet: Immunocytochemical detection of GH receptors in cumulus cells after 20 h maturation of bovine cumulus-enclosed oocytes. Specific localizations were detected by MAB 263 antibody and the red 3-amino-9-ethylcarbazole (AEC) chromophore. Nuclei were counterstained with hematoxylin. (A) Positive staining. Black arrows indicate GH receptor-specific immunoreaction. (B) Negative control performed by omitting the primary antibody. Original magnification: × 400.

    Article Snippet: For visualization of the specific staining, Vectastain ABC reagent and 3-amino-9-ethylcarbazole (AEC) substrate (all purchased from Vector Laboratories, Burlingame, CA, USA) were applied.

    Techniques: Staining, Negative Control

    Melanoma, dog. Immunohistochemical staining anti-FXR1, rabbit polyclonal anti-FXR1 antibody ab50841, ABC method, AEC chromogen. (A–B): oral melanoma, diffuse intense to moderate staining of neoplastic cells. (C–D): uveal melanoma, intense staining of neoplastic cells, multifocal to scattered. Abbreviations: FXR1, Fragile X mental retardation-related protein 1; ABC, avidin-biotin-peroxidase complex; AEC, 3-amino-9-ethylcarbazole. Scale bars = 12.5 μm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Immunohistochemical Expression of FXR1 in Canine Normal Tissues and Melanomas

    doi: 10.1369/0022155418766292

    Figure Lengend Snippet: Melanoma, dog. Immunohistochemical staining anti-FXR1, rabbit polyclonal anti-FXR1 antibody ab50841, ABC method, AEC chromogen. (A–B): oral melanoma, diffuse intense to moderate staining of neoplastic cells. (C–D): uveal melanoma, intense staining of neoplastic cells, multifocal to scattered. Abbreviations: FXR1, Fragile X mental retardation-related protein 1; ABC, avidin-biotin-peroxidase complex; AEC, 3-amino-9-ethylcarbazole. Scale bars = 12.5 μm.

    Article Snippet: The chromogen 3-amino-9-ethylcarbazole (AEC; Vector Laboratories) was applied for 15 min and, after rinsing in tap water, slides were counterstained with Mayer’s hematoxylin (Diapath srl; Martinengo, Italy) for 2 min.

    Techniques: Immunohistochemistry, Staining, Avidin-Biotin Assay

    Localization of PSA expression in the prostate and coagulating gland of the PSA1 transgenics by immunohistochemical staining. Formalin-fixed, paraffin-embedded tissue sections from the prostate ( A and B ) and coagulating gland/seminal vesicle ( E and F ) of a P1–9 transgenic, and prostate ( C and D ) and coagulating gland/seminal vesicle ( G and H ) of a nontransgenic control were incubated with rabbit anti-human PSA ( A , C , E , and G ) or control normal rabbit immunoglobulin ( B , D , F , and H ) followed by HRP-conjugated goat anti-rabbit Ig. Staining was visualized by adding the chromogen diaminobenzidine ( A – D ) or 3-amino-9-ethylcarbazole ( E – H ). ( E and G ) cg, coagulating gland; sv, seminal vesicle.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Tissue-specific expression of the human prostate-specific antigen gene in transgenic mice: Implications for tolerance and immunotherapy

    doi:

    Figure Lengend Snippet: Localization of PSA expression in the prostate and coagulating gland of the PSA1 transgenics by immunohistochemical staining. Formalin-fixed, paraffin-embedded tissue sections from the prostate ( A and B ) and coagulating gland/seminal vesicle ( E and F ) of a P1–9 transgenic, and prostate ( C and D ) and coagulating gland/seminal vesicle ( G and H ) of a nontransgenic control were incubated with rabbit anti-human PSA ( A , C , E , and G ) or control normal rabbit immunoglobulin ( B , D , F , and H ) followed by HRP-conjugated goat anti-rabbit Ig. Staining was visualized by adding the chromogen diaminobenzidine ( A – D ) or 3-amino-9-ethylcarbazole ( E – H ). ( E and G ) cg, coagulating gland; sv, seminal vesicle.

    Article Snippet: Following quenching of endogenous peroxidase, the sections were blocked with normal goat serum, stained with a 1:200 dilution of rabbit anti-human PSA (Dako) followed by goat anti-rabbit Ig conjugated to horse radish peroxidase (Dako), and visualized by adding metal-enhanced diaminobenzidine (Pierce) or 3-amino-9-ethylcarbazole (Vector Laboratories) as the substrate.

    Techniques: Expressing, Immunohistochemistry, Staining, Formalin-fixed Paraffin-Embedded, Transgenic Assay, Incubation

    Immunohistochemical demonstration of ERK/MAPK phosphorylation in astrocytic neoplasms. For each tumor, adjacent sections were immunolabeled with the phospho-specific ERK/MAPK antibody (pMAPK; left panels ) and a phospho-insensitive ERK/MAPK antibody (MAPK; right panels ). Elevated nuclear and cytoplasmic dp-ERK/MAPK immunoreactivity, relative to normal quiescent astrocytes, was detected in a subset of tumor cells within a variety of astrocytic neoplasms. In contrast, ERK/MAPK immunoreactivity was more uniformly expressed. A and B: Astrocytoma, WHO grade II. Some diffusely infiltrating astrocytoma cells show increased nuclear and cytoplasmic dp-ERK/MAPK immunoreactivity. Note the absence of labeling in adjacent normal neurons. ERK/MAPK protein is more evenly expressed in tumor cell cytoplasm and in neurons. C and D: Anaplastic astrocytoma, WHO grade III. A subset of gemistocytic tumor cells shows high cytoplasmic and nuclear dp-ERK/MAPK immunoreactivity. E and F: Ependymoma. A subpopulation of highly fibrillar ependymoma cells, especially those forming perivascular pseudorosettes, shows elevated dp-ERK/MAPK immunoreactivity; compare with the relatively uniform expression of ERK/MAPK protein. G and H: Glioblastoma multiforme (astrocytoma, WHO grade IV). Tumor cells near the hyperplastic microvasculature show the most intense nuclear and cytoplasmic pMAPK immunoreactivity. Some fine immunoreactive tumor cell processes appear to be oriented toward the microvasculature ( corner inset ). Total MAPK immunoreactivity is much more uniform, and not restricted to the perivascular tumor cells. I and J: Glioblastoma with giant cell transformation (astrocytoma, WHO grade IV). Only the large, bizarre, multinucleated tumor cells have detectable dp-ERK/MAPK immunoreactivity; the small cell population is completely negative. Note the absence of dp-ERK/MAPK immunoreactivity in a mitotic tumor cell ( left side ). In contrast, both small and large tumor cell populations show uniform ERK/MAPK protein immunoreactivity. Also, a mitotic cell is visible that has detectable MAPK immunoreactivity ( right side ). K to N: Selective dephosphorylation of ERK/MAPK within mitotic and cycling glioblastoma cells. K and L: Immunoreactivity for dp-ERK/MAPK is absent within individual mitotic tumor cells in two additional glioblastoma specimens; adjacent interphase cells show abundant cytoplasmic and nuclear immunoreactivity. M: Evidence that mitotic phosphoepitopes in general are not lost in routinely processed surgical specimens comes from immunolabeling with monoclonal antibody MPM-2, which recognizes a number of mitosis-specific phosphorylated proteins. Strong perichromosomal staining is present in mitotic cells. N: Double-label immunohistochemistry for dp-ERK/MAPK (red-brown) and Ki-67 (dark blue) reveals essentially complete non-co-localization. This suggests that cycling cells (those with nuclear Ki-67 immunolabeling) and those with activated ERK/MAPK (dp-ERK/MAPK immunoreactive) are generally mutually exclusive populations. A to M: Immunoperoxidase staining (DAB) with hematoxylin counterstain. N: Double-label immunoperoxidase with aminoethylcarbazole and Vector SG substrates; no counterstain. Scale bar in A represents 50 μm and applies to A to J and M ; bar in N represents 20 μm and applies to K , L , and N .

    Journal: The American Journal of Pathology

    Article Title: In Situ Visualization of Intratumor Growth Factor Signaling

    doi:

    Figure Lengend Snippet: Immunohistochemical demonstration of ERK/MAPK phosphorylation in astrocytic neoplasms. For each tumor, adjacent sections were immunolabeled with the phospho-specific ERK/MAPK antibody (pMAPK; left panels ) and a phospho-insensitive ERK/MAPK antibody (MAPK; right panels ). Elevated nuclear and cytoplasmic dp-ERK/MAPK immunoreactivity, relative to normal quiescent astrocytes, was detected in a subset of tumor cells within a variety of astrocytic neoplasms. In contrast, ERK/MAPK immunoreactivity was more uniformly expressed. A and B: Astrocytoma, WHO grade II. Some diffusely infiltrating astrocytoma cells show increased nuclear and cytoplasmic dp-ERK/MAPK immunoreactivity. Note the absence of labeling in adjacent normal neurons. ERK/MAPK protein is more evenly expressed in tumor cell cytoplasm and in neurons. C and D: Anaplastic astrocytoma, WHO grade III. A subset of gemistocytic tumor cells shows high cytoplasmic and nuclear dp-ERK/MAPK immunoreactivity. E and F: Ependymoma. A subpopulation of highly fibrillar ependymoma cells, especially those forming perivascular pseudorosettes, shows elevated dp-ERK/MAPK immunoreactivity; compare with the relatively uniform expression of ERK/MAPK protein. G and H: Glioblastoma multiforme (astrocytoma, WHO grade IV). Tumor cells near the hyperplastic microvasculature show the most intense nuclear and cytoplasmic pMAPK immunoreactivity. Some fine immunoreactive tumor cell processes appear to be oriented toward the microvasculature ( corner inset ). Total MAPK immunoreactivity is much more uniform, and not restricted to the perivascular tumor cells. I and J: Glioblastoma with giant cell transformation (astrocytoma, WHO grade IV). Only the large, bizarre, multinucleated tumor cells have detectable dp-ERK/MAPK immunoreactivity; the small cell population is completely negative. Note the absence of dp-ERK/MAPK immunoreactivity in a mitotic tumor cell ( left side ). In contrast, both small and large tumor cell populations show uniform ERK/MAPK protein immunoreactivity. Also, a mitotic cell is visible that has detectable MAPK immunoreactivity ( right side ). K to N: Selective dephosphorylation of ERK/MAPK within mitotic and cycling glioblastoma cells. K and L: Immunoreactivity for dp-ERK/MAPK is absent within individual mitotic tumor cells in two additional glioblastoma specimens; adjacent interphase cells show abundant cytoplasmic and nuclear immunoreactivity. M: Evidence that mitotic phosphoepitopes in general are not lost in routinely processed surgical specimens comes from immunolabeling with monoclonal antibody MPM-2, which recognizes a number of mitosis-specific phosphorylated proteins. Strong perichromosomal staining is present in mitotic cells. N: Double-label immunohistochemistry for dp-ERK/MAPK (red-brown) and Ki-67 (dark blue) reveals essentially complete non-co-localization. This suggests that cycling cells (those with nuclear Ki-67 immunolabeling) and those with activated ERK/MAPK (dp-ERK/MAPK immunoreactive) are generally mutually exclusive populations. A to M: Immunoperoxidase staining (DAB) with hematoxylin counterstain. N: Double-label immunoperoxidase with aminoethylcarbazole and Vector SG substrates; no counterstain. Scale bar in A represents 50 μm and applies to A to J and M ; bar in N represents 20 μm and applies to K , L , and N .

    Article Snippet: Double-label immunohistochemistry was performed sequentially using the peroxidase substrates aminoethylcarbazole and Vector SG, according to the manufacturer’s instructions (Vector Laboratories, Burlingame, CA).

    Techniques: Immunohistochemistry, Immunolabeling, Labeling, Expressing, Transformation Assay, De-Phosphorylation Assay, Staining, Immunoperoxidase Staining, Plasmid Preparation