diaminobenzidine  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    ImmPACT DAB Peroxidase HRP Substrate
    Description:
    ImmPACT DAB Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsHeat StablePermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated DAB stock solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This product is an entirely new formula for a diaminobenzidine based peroxidase substrate ImmPACT DAB produces a dark brown reaction product that is crisper and is 3 4 times more sensitive than the original Vector DAB substrate kit It can be used for both manual and automated staining methods The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB This product can also be used on blots
    Catalog Number:
    sk-4105
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Size:
    120 ml
    Buy from Supplier


    Structured Review

    Vector Laboratories diaminobenzidine
    ImmPACT DAB Peroxidase HRP Substrate
    ImmPACT DAB Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsHeat StablePermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated DAB stock solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This product is an entirely new formula for a diaminobenzidine based peroxidase substrate ImmPACT DAB produces a dark brown reaction product that is crisper and is 3 4 times more sensitive than the original Vector DAB substrate kit It can be used for both manual and automated staining methods The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB This product can also be used on blots
    https://www.bioz.com/result/diaminobenzidine/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors"

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors

    Journal: International Journal of Immunopathology and Pharmacology

    doi: 10.1177/0394632016681306

    Expression of VEGF-A in breast IDC and breast FA. Expression of VEGF-A in IDC samples before (a) and after (b) incubation with polyclonal activator (PA). Expression of VEGF-A in FA samples before (c) and after (d) incubation with PA. Immunohistochemical staining with 3,3′-diaminobenzidine (DAB; brown) as a substrate and additional staining with H E (400× magnification).
    Figure Legend Snippet: Expression of VEGF-A in breast IDC and breast FA. Expression of VEGF-A in IDC samples before (a) and after (b) incubation with polyclonal activator (PA). Expression of VEGF-A in FA samples before (c) and after (d) incubation with PA. Immunohistochemical staining with 3,3′-diaminobenzidine (DAB; brown) as a substrate and additional staining with H E (400× magnification).

    Techniques Used: Expressing, Incubation, Immunohistochemistry, Staining

    Related Articles

    Modification:

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Article Snippet: .. Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112) .. Incubator Centrifuge Cell culture laminar flow hood, vacuum suction, centrifuge, cell counter 2 L induction chamber Scissors (Roboz Surgical Instrument, catalog number: RS-5914) Fine forceps (Roboz Surgical Instrument, catalog number: RS-5132) Mouse housing and handling facility Isoflurane gas anesthetic system (VetEquip, catalog number: 922100) including a vaporizer, induction chamber, tubing, nose cones, and oxygen tank Glass bead sterilizer (GERMINATOR 500) (Roboz Surgical Instrument, catalog number: DS-401) Rechargeable shaver Space gel heating pads (Braintree Scientific, catalog number: SPGL) 25 μl, model 702 RN syringe (Hamilton, catalog number: 7636-01) Syringe guide 25 μl–500 μl/7000 SYR (Hamilton, catalog number: 14906)

    Recombinant:

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Article Snippet: .. Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112) .. Incubator Centrifuge Cell culture laminar flow hood, vacuum suction, centrifuge, cell counter 2 L induction chamber Scissors (Roboz Surgical Instrument, catalog number: RS-5914) Fine forceps (Roboz Surgical Instrument, catalog number: RS-5132) Mouse housing and handling facility Isoflurane gas anesthetic system (VetEquip, catalog number: 922100) including a vaporizer, induction chamber, tubing, nose cones, and oxygen tank Glass bead sterilizer (GERMINATOR 500) (Roboz Surgical Instrument, catalog number: DS-401) Rechargeable shaver Space gel heating pads (Braintree Scientific, catalog number: SPGL) 25 μl, model 702 RN syringe (Hamilton, catalog number: 7636-01) Syringe guide 25 μl–500 μl/7000 SYR (Hamilton, catalog number: 14906)

    Incubation:

    Article Title: A model of breast cancer heterogeneity reveals vascular mimicry as a driver of metastasis
    Article Snippet: The sections were then treated with high-temperature antigen retrieval in citrate buffer (pH 6), blocked with 2.5% ready-to-use normal horse serum from ImmPRESS Anti-Rabbit Ig (peroxidase) Polymer Detection Kit (MP-7401, Vector Laboratories) for 1 h and incubated with primary antibody against CD31 (1:400) (28364, Abcam) overnight at 4 °C. .. After washing, the slides were incubated with secondary antibody from the previous kit for 30 min, rinsed, and developed with chromogen ImmPACT DAB Peroxidase Substrate for staining (SK-4105, Vector Laboratories) until the desired intensity was achieved. .. Slides were then stained with Periodic Acid-Schiff (PAS) Kit (Sigma) according to manufacturer's instructions.

    Article Title: The absence of the leukotriene B4 receptor BLT1 attenuates peripheral inflammation and spinal nociceptive processing following intraplantar formalin injury
    Article Snippet: Sections were then incubated with a rabbit p-S133 rabbit monoclonal antibody for pCREB (1:400, Cell Signaling Technology, Danvers, MA, USA) containing 0.25% normal goat serum overnight at 4°C. .. The sections were incubated with biotinylated goat anti-rabbit IgG (1:200, Vector Laboratories, Burlingame, CA, USA) at room temperature for 1 h and then incubated in Vectastain Elite ABC Reagent (PK-6101, Vector Laboratories) for 1 h followed by incubation in a diaminobenzidine solution (ImmPACT™ DAB substrate, SK-4105, Vector Laboratories) for 1 min. ..

    Article Title: Inositol 1,4,5-triphosphate receptor is selectively expressed in cerebellum but not cerebellum-like structures of the elasmobranch fish, Leucoraja erinacea
    Article Snippet: Sections were incubated in primary antibody for 12–15 hours at room temperature followed by biotinylated secondary antibody (Vector Laboratories goatαrabbit Catalog # PK-6101 ) in blocking buffer for 30 minutes at room temperature. .. Slides were incubated in ABC reagent for 30 minutes, then in a DAB peroxidase substrate (Vector Laboratories ImmPACT DAB peroxidase (HRP) substrate Catalog # SK-4105 ) for two minutes and dehydrated in an ethyl alcohol series and cleared in xylene. .. A no-primary negative control was done for each protein.

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: Then the slices were incubated with the second biotinylated antibodies (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) for 30 min. .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Staining:

    Article Title: A model of breast cancer heterogeneity reveals vascular mimicry as a driver of metastasis
    Article Snippet: The sections were then treated with high-temperature antigen retrieval in citrate buffer (pH 6), blocked with 2.5% ready-to-use normal horse serum from ImmPRESS Anti-Rabbit Ig (peroxidase) Polymer Detection Kit (MP-7401, Vector Laboratories) for 1 h and incubated with primary antibody against CD31 (1:400) (28364, Abcam) overnight at 4 °C. .. After washing, the slides were incubated with secondary antibody from the previous kit for 30 min, rinsed, and developed with chromogen ImmPACT DAB Peroxidase Substrate for staining (SK-4105, Vector Laboratories) until the desired intensity was achieved. .. Slides were then stained with Periodic Acid-Schiff (PAS) Kit (Sigma) according to manufacturer's instructions.

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: Then the slices were incubated with the second biotinylated antibodies (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) for 30 min. .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Avidin-Biotin Assay:

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: Then the slices were incubated with the second biotinylated antibodies (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) for 30 min. .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Article Title: Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse/human IL-1β, IL-8, TNF-α, CCL2, CCL5, recombinant mouse IL-33 (rmIL-33), recombinant human IL-33 (rhIL-33), monoclonal anti-mouse IL-33-phycoerythrin conjugated Rat IgG2A, monoclonal anti-mouse CD40/TNFRSF5-Allophycocyanin conjugated Rat IgG2A, monoclonal anti-mouse CD40L/TNFSF5 Phycoerythrin conjugated Rat IgG2A antibodies and flow cytometry reagents were obtained from R & D Systems (Minneapolis, MN). .. ImmPRESS reagent anti-mouse Ig peroxidase, ImmPRESS reagent anti-rabbit Ig peroxidase kits, ImmPACT DAB peroxidase and avidin-biotin complex (ABC) kits were obtained from Vector Laboratories (Burlingame, CA). .. Recombinant α-synuclein (human) was from Enzo Life Sciences (Farmingdale, NY) and used with both human and mouse mast cells.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Vector Laboratories diaminobenzidine
    Expression of VEGF-A in breast IDC and breast FA. Expression of VEGF-A in IDC samples before (a) and after (b) incubation with polyclonal activator (PA). Expression of VEGF-A in FA samples before (c) and after (d) incubation with PA. Immunohistochemical staining with <t>3,3′-diaminobenzidine</t> (DAB; brown) as a substrate and additional staining with H E (400× magnification).
    Diaminobenzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diaminobenzidine/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Expression of VEGF-A in breast IDC and breast FA. Expression of VEGF-A in IDC samples before (a) and after (b) incubation with polyclonal activator (PA). Expression of VEGF-A in FA samples before (c) and after (d) incubation with PA. Immunohistochemical staining with 3,3′-diaminobenzidine (DAB; brown) as a substrate and additional staining with H E (400× magnification).

    Journal: International Journal of Immunopathology and Pharmacology

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors

    doi: 10.1177/0394632016681306

    Figure Lengend Snippet: Expression of VEGF-A in breast IDC and breast FA. Expression of VEGF-A in IDC samples before (a) and after (b) incubation with polyclonal activator (PA). Expression of VEGF-A in FA samples before (c) and after (d) incubation with PA. Immunohistochemical staining with 3,3′-diaminobenzidine (DAB; brown) as a substrate and additional staining with H E (400× magnification).

    Article Snippet: The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E.

    Techniques: Expressing, Incubation, Immunohistochemistry, Staining

    Ip3r1 immunoreactivity in the cerebellum. Schematic (left) shows the location of images in a, b and c. (a) ABC-DAB reaction using Ip3r1 antibody from Fig. 2 shows cellular localization in Purkinje cell somas and dendrites as well as some molecular layer interneurons of the skate cerebellum (Cb). (b) ABC-DAB reaction showing little to no Ip3r1 immunoreactivity in the dorsal nucleus, a cerebellum-like structure (CbLS). (c) No-primary negative control in skate cerebellar section. (d) Positive control in mouse cerebellar section shows immunoreactive Purkinje cell somas and dendrites. Scale bars equal 100μm. Abbreviations: FL, fibrous layer; GCL, granule cell layer; PCL, Purkinje cell layer; PZ, Principal zone; ML, molecular layer.

    Journal: Heliyon

    Article Title: Inositol 1,4,5-triphosphate receptor is selectively expressed in cerebellum but not cerebellum-like structures of the elasmobranch fish, Leucoraja erinacea

    doi: 10.1016/j.heliyon.2019.e01176

    Figure Lengend Snippet: Ip3r1 immunoreactivity in the cerebellum. Schematic (left) shows the location of images in a, b and c. (a) ABC-DAB reaction using Ip3r1 antibody from Fig. 2 shows cellular localization in Purkinje cell somas and dendrites as well as some molecular layer interneurons of the skate cerebellum (Cb). (b) ABC-DAB reaction showing little to no Ip3r1 immunoreactivity in the dorsal nucleus, a cerebellum-like structure (CbLS). (c) No-primary negative control in skate cerebellar section. (d) Positive control in mouse cerebellar section shows immunoreactive Purkinje cell somas and dendrites. Scale bars equal 100μm. Abbreviations: FL, fibrous layer; GCL, granule cell layer; PCL, Purkinje cell layer; PZ, Principal zone; ML, molecular layer.

    Article Snippet: Slides were incubated in ABC reagent for 30 minutes, then in a DAB peroxidase substrate (Vector Laboratories ImmPACT DAB peroxidase (HRP) substrate Catalog # SK-4105 ) for two minutes and dehydrated in an ethyl alcohol series and cleared in xylene.

    Techniques: Negative Control, Positive Control

    Myc-CaP orthotopic allograft and staining by H E and IHC A. Myc-CaP orthotopic allograft (arrow) in the mouse anterior prostate. Seminal vesicle (S.V.) and anterior prostate (A.P.). H E and IHC staining for Myc and androgen receptor (AR) of a Myc-CaP orthotopic allograft tumor; B. H E and IHC staining of a Myc-CaP orthotopic allograft tumor (T) and adjacent seminal vesicle containing secretions (S, bright pink staining); C. H E and IHC staining of a lung metastasis from a Myc-CaP orthotopic allograft. Positive Myc and AR staining, shown in brown, was visualized with an ImmPRESS Polymer Detection Kit and ImmPACT DAB (Vector Laboratories). IHC slides were counterstained with hematoxylin. Images at 10× magnification (20× inset) and black bars are 100 μm.

    Journal: Bio-protocol

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis

    doi: 10.21769/BioProtoc.2137

    Figure Lengend Snippet: Myc-CaP orthotopic allograft and staining by H E and IHC A. Myc-CaP orthotopic allograft (arrow) in the mouse anterior prostate. Seminal vesicle (S.V.) and anterior prostate (A.P.). H E and IHC staining for Myc and androgen receptor (AR) of a Myc-CaP orthotopic allograft tumor; B. H E and IHC staining of a Myc-CaP orthotopic allograft tumor (T) and adjacent seminal vesicle containing secretions (S, bright pink staining); C. H E and IHC staining of a lung metastasis from a Myc-CaP orthotopic allograft. Positive Myc and AR staining, shown in brown, was visualized with an ImmPRESS Polymer Detection Kit and ImmPACT DAB (Vector Laboratories). IHC slides were counterstained with hematoxylin. Images at 10× magnification (20× inset) and black bars are 100 μm.

    Article Snippet: Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112)

    Techniques: Staining, Immunohistochemistry, Plasmid Preparation

    Immunohistochemical analysis of PGC-1α and 8-OHdG in the kidneys of ADPKD model animals. (A) Representative IHC staining for PGC-1α in kidney tissue from 7-day-old Ksp-Cre PKD1 flox/+ and Ksp-Cre PKD1 flox/flox mice. Left, cortex of PKD1 flox/+ mouse kidney; middle and right, lower and higher magnifications of PKD1 flox/flox mouse kidney, respectively. The area surrounded by the dotted line in the higher-magnification image represents cyst-lining cells. The bar graph shows the relative ratio of staining intensity of diaminobenzidine (DAB) in normal tubules and cyst-lining cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (B) Representative IHC staining for PGC-1α in kidney tissue from 7-week-old Cy rats. Left, IHC results for controls ( +/+ ); right, IHC results for Cy/+ rats. The bar graph shows the relative ratio of staining intensity of diaminobenzidine (DAB) in normal tubules and cyst-lining cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (C) Representative IHC staining results for 8-OHdG in kidney tissue from 7-day-old Ksp-Cre PKD1 flox/flox mice. Left, kidney tissue from Ksp-Cre PKD1 flox/+ mice; middle and right, kidney tissue from Ksp-Cre PKD1 flox/flox mice and high magnification of the black box in the middle panel, respectively. The bar graph shows the relative ratio of staining intensity compared with that of normal tubules in Ksp-Cre PKD1 flox/flox mice. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (D) Representative IHC staining results for 8-OHdG in kidney tissue from 7-week-old Cy rats. *, representative cyst. The bar graph shows the relative ratio of staining intensity compared with that of normal tubules in Cy rats. The 8-OHdG staining intensity was increased in cyst-lining cells compared with normal tubular cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. Results are shown as the relative ratio. Results represent the means ± standard deviations. **, P

    Journal: Molecular and Cellular Biology

    Article Title: Mitochondrial Abnormality Facilitates Cyst Formation in Autosomal Dominant Polycystic Kidney Disease

    doi: 10.1128/MCB.00337-17

    Figure Lengend Snippet: Immunohistochemical analysis of PGC-1α and 8-OHdG in the kidneys of ADPKD model animals. (A) Representative IHC staining for PGC-1α in kidney tissue from 7-day-old Ksp-Cre PKD1 flox/+ and Ksp-Cre PKD1 flox/flox mice. Left, cortex of PKD1 flox/+ mouse kidney; middle and right, lower and higher magnifications of PKD1 flox/flox mouse kidney, respectively. The area surrounded by the dotted line in the higher-magnification image represents cyst-lining cells. The bar graph shows the relative ratio of staining intensity of diaminobenzidine (DAB) in normal tubules and cyst-lining cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (B) Representative IHC staining for PGC-1α in kidney tissue from 7-week-old Cy rats. Left, IHC results for controls ( +/+ ); right, IHC results for Cy/+ rats. The bar graph shows the relative ratio of staining intensity of diaminobenzidine (DAB) in normal tubules and cyst-lining cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (C) Representative IHC staining results for 8-OHdG in kidney tissue from 7-day-old Ksp-Cre PKD1 flox/flox mice. Left, kidney tissue from Ksp-Cre PKD1 flox/+ mice; middle and right, kidney tissue from Ksp-Cre PKD1 flox/flox mice and high magnification of the black box in the middle panel, respectively. The bar graph shows the relative ratio of staining intensity compared with that of normal tubules in Ksp-Cre PKD1 flox/flox mice. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (D) Representative IHC staining results for 8-OHdG in kidney tissue from 7-week-old Cy rats. *, representative cyst. The bar graph shows the relative ratio of staining intensity compared with that of normal tubules in Cy rats. The 8-OHdG staining intensity was increased in cyst-lining cells compared with normal tubular cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. Results are shown as the relative ratio. Results represent the means ± standard deviations. **, P

    Article Snippet: Sections of 3-μm thickness were incubated with the appropriate primary antibody and a Vectastain Elite ABC HRP kit (Vector Laboratories, Burlingame, CA) and developed by incubation with ImmPACT diaminobenzidine (DAB) peroxidase substrate (Vector Laboratories).

    Techniques: Immunohistochemistry, Pyrolysis Gas Chromatography, Staining, Mouse Assay