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DSMZ sk mel3
Melanoma cell lines and primary tumor cultures were efficiently infected and killed by VSV-GP. ( A ) The tropisms of VSV-GP and VSV wild-type for several human (A375, MDA-mB-435, MJS, NW1539, <t>SK-MEL3,</t> SK-MEL5) melanoma cell lines, one mouse (B16-OVA) melanoma cell line, and one dog (UCDK9-M1) melanoma cell line were analyzed. Cells were seeded as monolayers, and infected with 10-fold serial dilutions of the single cycle infective VSV*MQΔG-GP or VSV*MQΔG-G virus. BHK-21 cells were used as a reference. Sixteen hours post-infection, cells were analyzed for the percentage of GFP-positive cells via flow cytometry, and the titer for both viruses on each cell line was determined. The titers are given relative to the titer on BHK-21. Bars represent the means ± SEM of one representative of at least two independent experiments using duplicate or triplicate samples. For the control cell line, BHK-21, the mean and SEM of one representative experiment is shown; ( B ) monolayers of melanoma cell lines were infected with an MOI (multiplicity of infection) of 0.1 of VSV wild-type or VSV-GP in dodecaplicates. After 24 or 48 h, the viability of cells was determined using WST-1 assay. Values were normalized to the mock-infected sample, and represented as a percentage of surviving cells. Bars represent the mean ± SEM of one representative experiment of at least three independent experiments; ( C , D ) short-term cultures of human melanoma cells were seeded in 24-well plates, and 36 hours afterwards were infected with an MOI of 0.1 of VSV-GFP or VSV-GP-GFP. As control, normal melanocytes were used. Twenty-four hours post-infection, cells were analyzed in the fluorescence microscope for GFP positive, i.e., virus infected, ( C ) and living cells ( D ). Ten microscopic fields were assessed per condition. Bars represent mean ± SEM.
Sk Mel3, supplied by DSMZ, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sk mel3/product/DSMZ
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sk mel3 - by Bioz Stars, 2022-09
91/100 stars

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1) Product Images from "The Oncolytic Virus VSV-GP Is Effective against Malignant Melanoma"

Article Title: The Oncolytic Virus VSV-GP Is Effective against Malignant Melanoma

Journal: Viruses

doi: 10.3390/v10030108

Melanoma cell lines and primary tumor cultures were efficiently infected and killed by VSV-GP. ( A ) The tropisms of VSV-GP and VSV wild-type for several human (A375, MDA-mB-435, MJS, NW1539, SK-MEL3, SK-MEL5) melanoma cell lines, one mouse (B16-OVA) melanoma cell line, and one dog (UCDK9-M1) melanoma cell line were analyzed. Cells were seeded as monolayers, and infected with 10-fold serial dilutions of the single cycle infective VSV*MQΔG-GP or VSV*MQΔG-G virus. BHK-21 cells were used as a reference. Sixteen hours post-infection, cells were analyzed for the percentage of GFP-positive cells via flow cytometry, and the titer for both viruses on each cell line was determined. The titers are given relative to the titer on BHK-21. Bars represent the means ± SEM of one representative of at least two independent experiments using duplicate or triplicate samples. For the control cell line, BHK-21, the mean and SEM of one representative experiment is shown; ( B ) monolayers of melanoma cell lines were infected with an MOI (multiplicity of infection) of 0.1 of VSV wild-type or VSV-GP in dodecaplicates. After 24 or 48 h, the viability of cells was determined using WST-1 assay. Values were normalized to the mock-infected sample, and represented as a percentage of surviving cells. Bars represent the mean ± SEM of one representative experiment of at least three independent experiments; ( C , D ) short-term cultures of human melanoma cells were seeded in 24-well plates, and 36 hours afterwards were infected with an MOI of 0.1 of VSV-GFP or VSV-GP-GFP. As control, normal melanocytes were used. Twenty-four hours post-infection, cells were analyzed in the fluorescence microscope for GFP positive, i.e., virus infected, ( C ) and living cells ( D ). Ten microscopic fields were assessed per condition. Bars represent mean ± SEM.
Figure Legend Snippet: Melanoma cell lines and primary tumor cultures were efficiently infected and killed by VSV-GP. ( A ) The tropisms of VSV-GP and VSV wild-type for several human (A375, MDA-mB-435, MJS, NW1539, SK-MEL3, SK-MEL5) melanoma cell lines, one mouse (B16-OVA) melanoma cell line, and one dog (UCDK9-M1) melanoma cell line were analyzed. Cells were seeded as monolayers, and infected with 10-fold serial dilutions of the single cycle infective VSV*MQΔG-GP or VSV*MQΔG-G virus. BHK-21 cells were used as a reference. Sixteen hours post-infection, cells were analyzed for the percentage of GFP-positive cells via flow cytometry, and the titer for both viruses on each cell line was determined. The titers are given relative to the titer on BHK-21. Bars represent the means ± SEM of one representative of at least two independent experiments using duplicate or triplicate samples. For the control cell line, BHK-21, the mean and SEM of one representative experiment is shown; ( B ) monolayers of melanoma cell lines were infected with an MOI (multiplicity of infection) of 0.1 of VSV wild-type or VSV-GP in dodecaplicates. After 24 or 48 h, the viability of cells was determined using WST-1 assay. Values were normalized to the mock-infected sample, and represented as a percentage of surviving cells. Bars represent the mean ± SEM of one representative experiment of at least three independent experiments; ( C , D ) short-term cultures of human melanoma cells were seeded in 24-well plates, and 36 hours afterwards were infected with an MOI of 0.1 of VSV-GFP or VSV-GP-GFP. As control, normal melanocytes were used. Twenty-four hours post-infection, cells were analyzed in the fluorescence microscope for GFP positive, i.e., virus infected, ( C ) and living cells ( D ). Ten microscopic fields were assessed per condition. Bars represent mean ± SEM.

Techniques Used: Infection, Multiple Displacement Amplification, Flow Cytometry, Cytometry, WST-1 Assay, Fluorescence, Microscopy

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    DSMZ sk mel3
    Melanoma cell lines and primary tumor cultures were efficiently infected and killed by VSV-GP. ( A ) The tropisms of VSV-GP and VSV wild-type for several human (A375, MDA-mB-435, MJS, NW1539, <t>SK-MEL3,</t> SK-MEL5) melanoma cell lines, one mouse (B16-OVA) melanoma cell line, and one dog (UCDK9-M1) melanoma cell line were analyzed. Cells were seeded as monolayers, and infected with 10-fold serial dilutions of the single cycle infective VSV*MQΔG-GP or VSV*MQΔG-G virus. BHK-21 cells were used as a reference. Sixteen hours post-infection, cells were analyzed for the percentage of GFP-positive cells via flow cytometry, and the titer for both viruses on each cell line was determined. The titers are given relative to the titer on BHK-21. Bars represent the means ± SEM of one representative of at least two independent experiments using duplicate or triplicate samples. For the control cell line, BHK-21, the mean and SEM of one representative experiment is shown; ( B ) monolayers of melanoma cell lines were infected with an MOI (multiplicity of infection) of 0.1 of VSV wild-type or VSV-GP in dodecaplicates. After 24 or 48 h, the viability of cells was determined using WST-1 assay. Values were normalized to the mock-infected sample, and represented as a percentage of surviving cells. Bars represent the mean ± SEM of one representative experiment of at least three independent experiments; ( C , D ) short-term cultures of human melanoma cells were seeded in 24-well plates, and 36 hours afterwards were infected with an MOI of 0.1 of VSV-GFP or VSV-GP-GFP. As control, normal melanocytes were used. Twenty-four hours post-infection, cells were analyzed in the fluorescence microscope for GFP positive, i.e., virus infected, ( C ) and living cells ( D ). Ten microscopic fields were assessed per condition. Bars represent mean ± SEM.
    Sk Mel3, supplied by DSMZ, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sk mel3/product/DSMZ
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sk mel3 - by Bioz Stars, 2022-09
    91/100 stars
      Buy from Supplier

    90
    DSMZ sk mel3 dsmz
    Melanoma cell lines and primary tumor cultures were efficiently infected and killed by VSV-GP. ( A ) The tropisms of VSV-GP and VSV wild-type for several human (A375, MDA-mB-435, MJS, NW1539, <t>SK-MEL3,</t> SK-MEL5) melanoma cell lines, one mouse (B16-OVA) melanoma cell line, and one dog (UCDK9-M1) melanoma cell line were analyzed. Cells were seeded as monolayers, and infected with 10-fold serial dilutions of the single cycle infective VSV*MQΔG-GP or VSV*MQΔG-G virus. BHK-21 cells were used as a reference. Sixteen hours post-infection, cells were analyzed for the percentage of GFP-positive cells via flow cytometry, and the titer for both viruses on each cell line was determined. The titers are given relative to the titer on BHK-21. Bars represent the means ± SEM of one representative of at least two independent experiments using duplicate or triplicate samples. For the control cell line, BHK-21, the mean and SEM of one representative experiment is shown; ( B ) monolayers of melanoma cell lines were infected with an MOI (multiplicity of infection) of 0.1 of VSV wild-type or VSV-GP in dodecaplicates. After 24 or 48 h, the viability of cells was determined using WST-1 assay. Values were normalized to the mock-infected sample, and represented as a percentage of surviving cells. Bars represent the mean ± SEM of one representative experiment of at least three independent experiments; ( C , D ) short-term cultures of human melanoma cells were seeded in 24-well plates, and 36 hours afterwards were infected with an MOI of 0.1 of VSV-GFP or VSV-GP-GFP. As control, normal melanocytes were used. Twenty-four hours post-infection, cells were analyzed in the fluorescence microscope for GFP positive, i.e., virus infected, ( C ) and living cells ( D ). Ten microscopic fields were assessed per condition. Bars represent mean ± SEM.
    Sk Mel3 Dsmz, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sk mel3 dsmz/product/DSMZ
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sk mel3 dsmz - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

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    Melanoma cell lines and primary tumor cultures were efficiently infected and killed by VSV-GP. ( A ) The tropisms of VSV-GP and VSV wild-type for several human (A375, MDA-mB-435, MJS, NW1539, SK-MEL3, SK-MEL5) melanoma cell lines, one mouse (B16-OVA) melanoma cell line, and one dog (UCDK9-M1) melanoma cell line were analyzed. Cells were seeded as monolayers, and infected with 10-fold serial dilutions of the single cycle infective VSV*MQΔG-GP or VSV*MQΔG-G virus. BHK-21 cells were used as a reference. Sixteen hours post-infection, cells were analyzed for the percentage of GFP-positive cells via flow cytometry, and the titer for both viruses on each cell line was determined. The titers are given relative to the titer on BHK-21. Bars represent the means ± SEM of one representative of at least two independent experiments using duplicate or triplicate samples. For the control cell line, BHK-21, the mean and SEM of one representative experiment is shown; ( B ) monolayers of melanoma cell lines were infected with an MOI (multiplicity of infection) of 0.1 of VSV wild-type or VSV-GP in dodecaplicates. After 24 or 48 h, the viability of cells was determined using WST-1 assay. Values were normalized to the mock-infected sample, and represented as a percentage of surviving cells. Bars represent the mean ± SEM of one representative experiment of at least three independent experiments; ( C , D ) short-term cultures of human melanoma cells were seeded in 24-well plates, and 36 hours afterwards were infected with an MOI of 0.1 of VSV-GFP or VSV-GP-GFP. As control, normal melanocytes were used. Twenty-four hours post-infection, cells were analyzed in the fluorescence microscope for GFP positive, i.e., virus infected, ( C ) and living cells ( D ). Ten microscopic fields were assessed per condition. Bars represent mean ± SEM.

    Journal: Viruses

    Article Title: The Oncolytic Virus VSV-GP Is Effective against Malignant Melanoma

    doi: 10.3390/v10030108

    Figure Lengend Snippet: Melanoma cell lines and primary tumor cultures were efficiently infected and killed by VSV-GP. ( A ) The tropisms of VSV-GP and VSV wild-type for several human (A375, MDA-mB-435, MJS, NW1539, SK-MEL3, SK-MEL5) melanoma cell lines, one mouse (B16-OVA) melanoma cell line, and one dog (UCDK9-M1) melanoma cell line were analyzed. Cells were seeded as monolayers, and infected with 10-fold serial dilutions of the single cycle infective VSV*MQΔG-GP or VSV*MQΔG-G virus. BHK-21 cells were used as a reference. Sixteen hours post-infection, cells were analyzed for the percentage of GFP-positive cells via flow cytometry, and the titer for both viruses on each cell line was determined. The titers are given relative to the titer on BHK-21. Bars represent the means ± SEM of one representative of at least two independent experiments using duplicate or triplicate samples. For the control cell line, BHK-21, the mean and SEM of one representative experiment is shown; ( B ) monolayers of melanoma cell lines were infected with an MOI (multiplicity of infection) of 0.1 of VSV wild-type or VSV-GP in dodecaplicates. After 24 or 48 h, the viability of cells was determined using WST-1 assay. Values were normalized to the mock-infected sample, and represented as a percentage of surviving cells. Bars represent the mean ± SEM of one representative experiment of at least three independent experiments; ( C , D ) short-term cultures of human melanoma cells were seeded in 24-well plates, and 36 hours afterwards were infected with an MOI of 0.1 of VSV-GFP or VSV-GP-GFP. As control, normal melanocytes were used. Twenty-four hours post-infection, cells were analyzed in the fluorescence microscope for GFP positive, i.e., virus infected, ( C ) and living cells ( D ). Ten microscopic fields were assessed per condition. Bars represent mean ± SEM.

    Article Snippet: SK-MEL3 (from DSMZ) were maintained in McCoy’s A5 medium (Gibco) supplemented with 10% FCS, 100 U/mL penicillin, and 100 mg/mL Streptomycin.

    Techniques: Infection, Multiple Displacement Amplification, Flow Cytometry, Cytometry, WST-1 Assay, Fluorescence, Microscopy