sites bamhi  (New England Biolabs)


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    Name:
    BamHI
    Description:
    BamHI 50 000 units
    Catalog Number:
    r0136l
    Price:
    249
    Size:
    50 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs sites bamhi
    BamHI
    BamHI 50 000 units
    https://www.bioz.com/result/sites bamhi/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sites bamhi - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility
    Article Snippet: .. The PCR product was digested with HindIII and BamHI and ligated into pMAL-cR1 v. 2 digested with the same enzymes (New England Biolabs, Inc.). .. The construct was transformed into E. coli XL1 blue, and, for expression of the maltose-binding∷hydin fusion protein, into BL21.

    Clone Assay:

    Article Title: The development and application of new crystallization method for tobacco mosaic virus coat protein
    Article Snippet: .. Both plasmid PGEX-6P-1 (Novagen) and CP were digested with BamH I (NEB, 10 units/μL)/Xho I (NEB, 10 units/μL) and cloned into the same sites in PGEX-6P-1 (PGEX-6P-1-WT-GST-TMV-CP32 ). ..

    other:

    Article Title: Probing hyper-negatively supercoiled mini-circles with nucleases and DNA binding proteins
    Article Snippet: Escherichia coli topoisomerase I ( Ec TopoI), T4 polynucleotide kinase (PNK), calf intestinal phosphatase, T4 DNA ligase, DNAse I, BamHI, BglII and HindIII were from New England Biolabs.

    Plasmid Preparation:

    Article Title: The development and application of new crystallization method for tobacco mosaic virus coat protein
    Article Snippet: .. Both plasmid PGEX-6P-1 (Novagen) and CP were digested with BamH I (NEB, 10 units/μL)/Xho I (NEB, 10 units/μL) and cloned into the same sites in PGEX-6P-1 (PGEX-6P-1-WT-GST-TMV-CP32 ). ..

    Article Title: Recognition of DNA Termini by the C-Terminal Region of the Ku80 and the DNA-Dependent Protein Kinase Catalytic Subunit
    Article Snippet: .. Kinase assays using plasmid DNA substrates were performed with pcDNA3.1 digested with either XhoI, BamHI, EcoRV, and KpnI or pCAG-GFP digested with XbaI or EcoRI (New England Biolabs). .. The specific sequences recognized by the restriction enzymes and DNA termini generated are shown in .

    Article Title: Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae
    Article Snippet: .. The ura3 -deficient S. cerevisiae strain BJ5465 ( α ura3–52 trp1 leu2Δ1 his3Δ200 pep4::HIS2 prb1Δ1.6R can1 GAL1 ) was obtained from LGCPromochem, the NucleoSpin Plasmid kit was purchased from Macherey-Nagel, and the restriction enzymes BamHI, NheI, SpeI, SacI, and NotI from New England Biolabs. ..

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    New England Biolabs bamhi restriction sites
    Sperm transfected with E6/E7 plasmid penetrate oocytes. a . Scheme of the recombinant plasmid pIRES2-AcGFP1-E6E7. E6/E7 genes have been amplified (1032 bp) from plasmid p1321 HPV-16 E6/E7 by PCR and subcloned to plasmid pIRES2-AcGFP1 between <t>SalI</t> and <t>BamHI</t> restriction sites. b . PCR for HPV E6/E7 genes from transfected sperm. Lane M: DNA marker (100 bp); 1: sperm transfected with recombinant E6/E7 plasmid; 2: negative control (no template); 3: sperm transfected only with Lipofectamine 2000; 4: positive control (pIRES2-AcGFP1-E6E7 plasmid). c . Mean number of human sperm penetrated per hamster oocyte in control and sperm transfected with HPV-16 E6/E7 plasmid. d . Hamster oocytes penetrated by control sperm and sperm transfected with HPV16 E6/E7 plasmid in bright field (BF, upper panel) and fluorescence (FL, lower panel) using SYBR green DNA stain.
    Bamhi Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction sites/product/New England Biolabs
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
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    89
    New England Biolabs bamh i site
    Schematic representation of the LV cloning strategy. There is a <t>BamH</t> I clone site at the 5'-end of the original vectors pcDNA3.1/V5-His-Snk/hPlk2, and pEGFP-N1, respectively. A BamH I clone site was inserted at the 3'-ends of EGFP and hPlk2 WT by SDM, respectively. Then, K111M, T239D and T239V mutants were created through SDM using hPlk2 WT gene as template. The inserts were digested with BamH I, and purified. At the same time, <t>pWPI</t> vector was digested by BamH I, and treated with CIP to protect the self-circularization of the vector DNA. Finally, LVs were cloned through ligation and transformation.
    Bamh I Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    New England Biolabs bamh1 restriction site underlined
    Schematic representation of recombinant parvoviral vector constructions. The upper diagram depicts the empty vector DNA clone PVH1-Δ800 which has 800-bp deletion in the VP coding region and carries a multiple cloning sequence (MluI/SmaI polylinker) at the VP2 translation initiation site. DNA inserts encoding for GFP or catalytic yCD were introduced in polylinker using XhoI and <t>BamH1</t> restriction enzymes. The resulting rPVH1-GFP and rPVH1-yCD plasmids were used for the production of corresponding non-replicative recombinant parvoviruses.
    Bamh1 Restriction Site Underlined, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Sperm transfected with E6/E7 plasmid penetrate oocytes. a . Scheme of the recombinant plasmid pIRES2-AcGFP1-E6E7. E6/E7 genes have been amplified (1032 bp) from plasmid p1321 HPV-16 E6/E7 by PCR and subcloned to plasmid pIRES2-AcGFP1 between SalI and BamHI restriction sites. b . PCR for HPV E6/E7 genes from transfected sperm. Lane M: DNA marker (100 bp); 1: sperm transfected with recombinant E6/E7 plasmid; 2: negative control (no template); 3: sperm transfected only with Lipofectamine 2000; 4: positive control (pIRES2-AcGFP1-E6E7 plasmid). c . Mean number of human sperm penetrated per hamster oocyte in control and sperm transfected with HPV-16 E6/E7 plasmid. d . Hamster oocytes penetrated by control sperm and sperm transfected with HPV16 E6/E7 plasmid in bright field (BF, upper panel) and fluorescence (FL, lower panel) using SYBR green DNA stain.

    Journal: PLoS ONE

    Article Title: Mechanism of Human Papillomavirus Binding to Human Spermatozoa and Fertilizing Ability of Infected Spermatozoa

    doi: 10.1371/journal.pone.0015036

    Figure Lengend Snippet: Sperm transfected with E6/E7 plasmid penetrate oocytes. a . Scheme of the recombinant plasmid pIRES2-AcGFP1-E6E7. E6/E7 genes have been amplified (1032 bp) from plasmid p1321 HPV-16 E6/E7 by PCR and subcloned to plasmid pIRES2-AcGFP1 between SalI and BamHI restriction sites. b . PCR for HPV E6/E7 genes from transfected sperm. Lane M: DNA marker (100 bp); 1: sperm transfected with recombinant E6/E7 plasmid; 2: negative control (no template); 3: sperm transfected only with Lipofectamine 2000; 4: positive control (pIRES2-AcGFP1-E6E7 plasmid). c . Mean number of human sperm penetrated per hamster oocyte in control and sperm transfected with HPV-16 E6/E7 plasmid. d . Hamster oocytes penetrated by control sperm and sperm transfected with HPV16 E6/E7 plasmid in bright field (BF, upper panel) and fluorescence (FL, lower panel) using SYBR green DNA stain.

    Article Snippet: The PCR mixture consisted of 5 µL Expand high fidelity buffer with MgCl2 , 1 µL of PCR grade nucleotide mix (10×), 20 pmol of each primer with SalI and BamHI restriction sites (New England Biolabs, Ipswich, MA) including forward: 5′-GTGCGCGTCGACGTGATGCACCAAAAGAGAACTG-3′ and reverse: 5′-GTGCGCGGATCCGTGTGGTTTCTGAGAACAGATG-3′ , 0,75 µL Expand high fidelity enzyme mix (Expand High Fidelity PCR System dNTPack, Roche Applied Science, Mannheim, Germany) and sterile H2 O in a final volume of 50 μL.

    Techniques: Transfection, Plasmid Preparation, Recombinant, Amplification, Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Fluorescence, SYBR Green Assay, Staining

    Schematic representation of the LV cloning strategy. There is a BamH I clone site at the 5'-end of the original vectors pcDNA3.1/V5-His-Snk/hPlk2, and pEGFP-N1, respectively. A BamH I clone site was inserted at the 3'-ends of EGFP and hPlk2 WT by SDM, respectively. Then, K111M, T239D and T239V mutants were created through SDM using hPlk2 WT gene as template. The inserts were digested with BamH I, and purified. At the same time, pWPI vector was digested by BamH I, and treated with CIP to protect the self-circularization of the vector DNA. Finally, LVs were cloned through ligation and transformation.

    Journal: Scientific Reports

    Article Title: Quantitative assessment on the cloning efficiencies of lentiviral transfer vectors with a unique clone site

    doi: 10.1038/srep00415

    Figure Lengend Snippet: Schematic representation of the LV cloning strategy. There is a BamH I clone site at the 5'-end of the original vectors pcDNA3.1/V5-His-Snk/hPlk2, and pEGFP-N1, respectively. A BamH I clone site was inserted at the 3'-ends of EGFP and hPlk2 WT by SDM, respectively. Then, K111M, T239D and T239V mutants were created through SDM using hPlk2 WT gene as template. The inserts were digested with BamH I, and purified. At the same time, pWPI vector was digested by BamH I, and treated with CIP to protect the self-circularization of the vector DNA. Finally, LVs were cloned through ligation and transformation.

    Article Snippet: Preparation of vector and insert DNA The bicistronic LV pWPI (Addgene plasmid 12254) was modified by creating a BamH I site at 3502 nt and replacing EGFP sequence with Neo, to form pWPI/Neo/BamH I. pWPI/Neo/BamH I DNA was digested with BamH I (NEW ENGLAND BioLabs), then divided into two aliquots, one aliquot of the vector DNA was used directly for ligation, and another aliquot was treated with CIP (NEW ENGLAND BioLabs) to remove the 5’-phosphate groups ( ) as follows: in a 300µl reaction, containing digested pWPI/Neo DNA (about 15 µg), 50 U CIP in 1 X NEBuffer 3, at 37°C water bath for 1 hours.

    Techniques: Clone Assay, Purification, Plasmid Preparation, Ligation, Transformation Assay

    Schematic representation of recombinant parvoviral vector constructions. The upper diagram depicts the empty vector DNA clone PVH1-Δ800 which has 800-bp deletion in the VP coding region and carries a multiple cloning sequence (MluI/SmaI polylinker) at the VP2 translation initiation site. DNA inserts encoding for GFP or catalytic yCD were introduced in polylinker using XhoI and BamH1 restriction enzymes. The resulting rPVH1-GFP and rPVH1-yCD plasmids were used for the production of corresponding non-replicative recombinant parvoviruses.

    Journal: PLoS ONE

    Article Title: Oncosuppressive Suicide Gene Virotherapy "PVH1-yCD/5-FC" for Pancreatic Peritoneal Carcinomatosis Treatment: NF?B and Akt/PI3K Involvement

    doi: 10.1371/journal.pone.0070594

    Figure Lengend Snippet: Schematic representation of recombinant parvoviral vector constructions. The upper diagram depicts the empty vector DNA clone PVH1-Δ800 which has 800-bp deletion in the VP coding region and carries a multiple cloning sequence (MluI/SmaI polylinker) at the VP2 translation initiation site. DNA inserts encoding for GFP or catalytic yCD were introduced in polylinker using XhoI and BamH1 restriction enzymes. The resulting rPVH1-GFP and rPVH1-yCD plasmids were used for the production of corresponding non-replicative recombinant parvoviruses.

    Article Snippet: The yCD gene was isolated from yeast genomic DNA (strain D4916 from Sigma-Aldrich) using specific probes: Forward: 5′-att ctcgag c gccaccatgg tgacagggggaatg-3′ containing a Kozak sequence (bold type) and XhoI restriction site (underlined), and Reverse: 5′-att ggatcc ctactcaccaatatcttcaaacc-3′ containing BamH1 restriction site (underlined), and was phosphorylated with T4 Polynucleotide Kinase (New England Biolabs-Ozyme, Montigny-Le-Bretonneux; France).

    Techniques: Recombinant, Plasmid Preparation, Clone Assay, Sequencing