siport lipid transfection agent  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    siPORT Amine Transfection Agent
    Description:
    A polyamine based Ambion siRNA transfection agent that can be used to transfect cells during subculturing saving a full day of valuable time without increasing toxicity It is supplied in one tube containing 0 4 mL A successful siRNA experiment requires the appropriate transfection agent for your cells and one that is optimized for siRNA delivery The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected RNA vs DNA single stranded vs double stranded and the cells being used Accessory Products siPORT NeoFX Transfection Agent SKU s AM4511 and AM4510 a cationic and neutral lipid mixture is also available For testing which transfection agent is best with your system the Silencer siRNA Transfection II Kit SKU AM1631 is recommended It contains the two different transfection agents along with controls for transfection optimization
    Catalog Number:
    am4502
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|RNAi Transfection|Synthetic siRNA Transfection|Transfection
    Buy from Supplier


    Structured Review

    Thermo Fisher siport lipid transfection agent
    A polyamine based Ambion siRNA transfection agent that can be used to transfect cells during subculturing saving a full day of valuable time without increasing toxicity It is supplied in one tube containing 0 4 mL A successful siRNA experiment requires the appropriate transfection agent for your cells and one that is optimized for siRNA delivery The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected RNA vs DNA single stranded vs double stranded and the cells being used Accessory Products siPORT NeoFX Transfection Agent SKU s AM4511 and AM4510 a cationic and neutral lipid mixture is also available For testing which transfection agent is best with your system the Silencer siRNA Transfection II Kit SKU AM1631 is recommended It contains the two different transfection agents along with controls for transfection optimization
    https://www.bioz.com/result/siport lipid transfection agent/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    siport lipid transfection agent - by Bioz Stars, 2021-03
    95/100 stars

    Images

    Related Articles

    Positive Control:

    Article Title: Pim-2 phosphorylation of p21Cip1/WAF1 enhances its stability and inhibits cell proliferation in HCT116 cells
    Article Snippet: Transfections were carried out with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. .. Silencer validated siRNA to Pim-2, Silencer GAPDH siRNA (positive control) and Silencer negative control siRNA( Ambion) were transfected into HCT116 and DU145 cells using SiPORT lipid siRNA transfection reagent (Ambion) and cells were cultured for 48h before harvesting for analysis. .. For cycloheximide treatment, cells were treated with a concentration of 25μg/ml for the indicated time.

    Negative Control:

    Article Title: Pim-2 phosphorylation of p21Cip1/WAF1 enhances its stability and inhibits cell proliferation in HCT116 cells
    Article Snippet: Transfections were carried out with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. .. Silencer validated siRNA to Pim-2, Silencer GAPDH siRNA (positive control) and Silencer negative control siRNA( Ambion) were transfected into HCT116 and DU145 cells using SiPORT lipid siRNA transfection reagent (Ambion) and cells were cultured for 48h before harvesting for analysis. .. For cycloheximide treatment, cells were treated with a concentration of 25μg/ml for the indicated time.

    Article Title: Epigenetic changes and nuclear factor-κB activation, but not microRNA-224, downregulate Raf-1 kinase inhibitor protein in triple-negative breast cancer SUM 159 cells
    Article Snippet: Blood DNA obtained from a healthy, young individual was used as an unmethylated negative control following receipt of written informed consent. .. Cell transfection with anti-miR microRNA (miRNA) inhibitor and pre-miR miRNA precursor SUM 159 cells were transfected with 30 nM pre-miR-224 miRNA precursor, anti-miR-224 miRNA inhibitor and relative random sequences (pre-miR negative control) as negative controls, using siPORT™ Amine Transfection Agent (all these reagents were from Ambion Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions. .. Extraction of cellular RNA and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from clinical samples and cell lines using TRIzol reagent (Invitrogen Life Technologies).

    Article Title: Epigenetic changes and nuclear factor-κB activation, but not microRNA-224, downregulate Raf-1 kinase inhibitor protein in triple-negative breast cancer SUM 159 cells
    Article Snippet: Blood DNA obtained from a healthy, young individual was used as an unmethylated negative control following receipt of written informed consent. .. SUM 159 cells were transfected with 30 nM pre-miR-224 miRNA precursor, anti-miR-224 miRNA inhibitor and relative random sequences (pre-miR negative control) as negative controls, using siPORT™ Amine Transfection Agent (all these reagents were from Ambion Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions. .. Total RNA was extracted from clinical samples and cell lines using TRIzol reagent (Invitrogen Life Technologies).

    Transfection:

    Article Title: Pim-2 phosphorylation of p21Cip1/WAF1 enhances its stability and inhibits cell proliferation in HCT116 cells
    Article Snippet: Transfections were carried out with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. .. Silencer validated siRNA to Pim-2, Silencer GAPDH siRNA (positive control) and Silencer negative control siRNA( Ambion) were transfected into HCT116 and DU145 cells using SiPORT lipid siRNA transfection reagent (Ambion) and cells were cultured for 48h before harvesting for analysis. .. For cycloheximide treatment, cells were treated with a concentration of 25μg/ml for the indicated time.

    Article Title: Epigenetic changes and nuclear factor-κB activation, but not microRNA-224, downregulate Raf-1 kinase inhibitor protein in triple-negative breast cancer SUM 159 cells
    Article Snippet: Blood DNA obtained from a healthy, young individual was used as an unmethylated negative control following receipt of written informed consent. .. Cell transfection with anti-miR microRNA (miRNA) inhibitor and pre-miR miRNA precursor SUM 159 cells were transfected with 30 nM pre-miR-224 miRNA precursor, anti-miR-224 miRNA inhibitor and relative random sequences (pre-miR negative control) as negative controls, using siPORT™ Amine Transfection Agent (all these reagents were from Ambion Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions. .. Extraction of cellular RNA and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from clinical samples and cell lines using TRIzol reagent (Invitrogen Life Technologies).

    Article Title: Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy
    Article Snippet: .. Live cell confocal imagingFluorescence imaging was performed 24 h after cell transfection. .. Cells were kept at RT in Dulbecco’s PBS (DPBS; Life Technologies) and imaged on a Fluoview FV1000 microscope, which is an inverted IX81 confocal system (Olympus, Tokyo, Japan), using a 60X, NA 1.35 oil immersion UPlanSApo objective (Olympus).

    Article Title: Formation of GFAP Cytoplasmic Inclusions in Astrocytes and Their Disaggregation by ?B-Crystallin
    Article Snippet: .. Transfections were performed using a polyamine transfection reagent (TransIT LT-1, PanVera, Madison, WI) according to the manufacturer’s protocol. .. In most experiments, 1 μg of GFAP expression vectors was used for each culture well.

    Article Title: The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes
    Article Snippet: After overnight incubation, when cells reached 70% confluence, they were transfected with specific shRNAs against TCF-4 with Lipofectamine TM 2000 reagent (Invitrogen, San Diego, CA, USA) according to the manufacturer’s instructions. .. No cell toxicity from the transfection agent was detected. .. The transfection efficiency was evaluated with fluorescein by fluorescence microscopy 24 h after transfection.

    Article Title: Epigenetic changes and nuclear factor-κB activation, but not microRNA-224, downregulate Raf-1 kinase inhibitor protein in triple-negative breast cancer SUM 159 cells
    Article Snippet: Blood DNA obtained from a healthy, young individual was used as an unmethylated negative control following receipt of written informed consent. .. SUM 159 cells were transfected with 30 nM pre-miR-224 miRNA precursor, anti-miR-224 miRNA inhibitor and relative random sequences (pre-miR negative control) as negative controls, using siPORT™ Amine Transfection Agent (all these reagents were from Ambion Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions. .. Total RNA was extracted from clinical samples and cell lines using TRIzol reagent (Invitrogen Life Technologies).

    Article Title: Rapid activation of ERK1/2 and AKT in human breast cancer cells by cadmium
    Article Snippet: For siRNA studies, a smart pool of double-stranded siRNA against human ERα (hERα; M-003401-02) and non-specific siRNA (D-001206-01-05) were obtained from Dharmacon Tech (Lafayette, CO). .. The siRNA transfection reagent siPORT lipid was purchased from Ambion (Austin, TX). ..

    Article Title: MicroRNA-1236-3p inhibits human osteosarcoma growth
    Article Snippet: Cell transfection At 50–60% confluence, HOS and U2OS cells were transfected with miR-1236-3p mimic, negative control (NC) mimic, inhibitor NC, miR-1236-3p inhibitor, Wnt3a-small interfering (si)RNA or NC siRNA (10 nM final concentration; Shanghai GenePharma Co., Ltd.) using Lipofectamine® 3000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. Subsequent experiments were performed 48 h after cell transfection. .. Fluorescence in situ hybridization (FISH)FAM (488)-labeled, locked nucleic acid miR-1236-3p probes were designed and synthesized by Wuhan Servicebio Technology Co., Ltd.

    Cell Culture:

    Article Title: Pim-2 phosphorylation of p21Cip1/WAF1 enhances its stability and inhibits cell proliferation in HCT116 cells
    Article Snippet: Transfections were carried out with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. .. Silencer validated siRNA to Pim-2, Silencer GAPDH siRNA (positive control) and Silencer negative control siRNA( Ambion) were transfected into HCT116 and DU145 cells using SiPORT lipid siRNA transfection reagent (Ambion) and cells were cultured for 48h before harvesting for analysis. .. For cycloheximide treatment, cells were treated with a concentration of 25μg/ml for the indicated time.

    Imaging:

    Article Title: Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy
    Article Snippet: .. Live cell confocal imagingFluorescence imaging was performed 24 h after cell transfection. .. Cells were kept at RT in Dulbecco’s PBS (DPBS; Life Technologies) and imaged on a Fluoview FV1000 microscope, which is an inverted IX81 confocal system (Olympus, Tokyo, Japan), using a 60X, NA 1.35 oil immersion UPlanSApo objective (Olympus).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Thermo Fisher siport lipid sirna transfection reagent
    Pim-2 knockdown reduces endogenous level of p21 HCT116 and DU145 cells were transfected with <t>siRNA</t> for pim-2 . Two days after <t>transfection,</t> cell lysates were prepared and analyzed by the indicated antibodies to determine Pim-2 and p21 protein levels. Actin was used as a protein loading control.
    Siport Lipid Sirna Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/siport lipid sirna transfection reagent/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    siport lipid sirna transfection reagent - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    95
    Thermo Fisher lipid based transfection reagent siport neofx
    Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with <t>transfection</t> reagent <t>siPORT</t> <t>NeoFX</t> alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p
    Lipid Based Transfection Reagent Siport Neofx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent siport neofx/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent siport neofx - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    Image Search Results


    Pim-2 knockdown reduces endogenous level of p21 HCT116 and DU145 cells were transfected with siRNA for pim-2 . Two days after transfection, cell lysates were prepared and analyzed by the indicated antibodies to determine Pim-2 and p21 protein levels. Actin was used as a protein loading control.

    Journal: The international journal of biochemistry & cell biology

    Article Title: Pim-2 phosphorylation of p21Cip1/WAF1 enhances its stability and inhibits cell proliferation in HCT116 cells

    doi: 10.1016/j.biocel.2010.03.012

    Figure Lengend Snippet: Pim-2 knockdown reduces endogenous level of p21 HCT116 and DU145 cells were transfected with siRNA for pim-2 . Two days after transfection, cell lysates were prepared and analyzed by the indicated antibodies to determine Pim-2 and p21 protein levels. Actin was used as a protein loading control.

    Article Snippet: Silencer validated siRNA to Pim-2, Silencer GAPDH siRNA (positive control) and Silencer negative control siRNA( Ambion) were transfected into HCT116 and DU145 cells using SiPORT lipid siRNA transfection reagent (Ambion) and cells were cultured for 48h before harvesting for analysis.

    Techniques: Transfection

    Effect of suppression of ERα expression on Cd- and E2-induced activation of ERK1/2 in MCF-7 cells. Forty eight hours after transfection with the non-specific or specific hERα siRNA, the quiescent cells were treated with either 10 μM Cd or 10 nM E2 for 2.5 min. (A, C) Phosphorylation of ERK1/2, and (B, D) phosphorylation of AKT. Data are presented as mean±SD from three independent experiments. *Significantly higher than the respective control cells ( p

    Journal: Toxicology and applied pharmacology

    Article Title: Rapid activation of ERK1/2 and AKT in human breast cancer cells by cadmium

    doi: 10.1016/j.taap.2007.12.017

    Figure Lengend Snippet: Effect of suppression of ERα expression on Cd- and E2-induced activation of ERK1/2 in MCF-7 cells. Forty eight hours after transfection with the non-specific or specific hERα siRNA, the quiescent cells were treated with either 10 μM Cd or 10 nM E2 for 2.5 min. (A, C) Phosphorylation of ERK1/2, and (B, D) phosphorylation of AKT. Data are presented as mean±SD from three independent experiments. *Significantly higher than the respective control cells ( p

    Article Snippet: The siRNA transfection reagent siPORT lipid was purchased from Ambion (Austin, TX).

    Techniques: Expressing, Activation Assay, Transfection

    Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with transfection reagent siPORT NeoFX alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p

    Journal: BMC Cell Biology

    Article Title: Mimitin - a novel cytokine-regulated mitochondrial protein

    doi: 10.1186/1471-2121-10-23

    Figure Lengend Snippet: Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with transfection reagent siPORT NeoFX alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p

    Article Snippet: Lipid-based transfection reagent siPORT NeoFX (4 μl/well) from Ambion and siRNAs (50 nM final concentration) were separately diluted in 50 μl OPTIMEM and then mixed together.

    Techniques: Transfection, Positive Control, Negative Control, Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Effect of mimitin gene silencing on caspase 3/7 activities . HepG2 cells were transfected with mimitin-siRNA, GAPDH-siRNA, negative control siRNA, or transfection reagent (siPORT NeoFX) (blank). Apoptosis was induced 72 h post transfection by treatment of cells with TNF/CHX for 5 h. Caspase 3/7 activity was measured using Caspase-GloTM3/7 assay kit. A. Changes in caspase activity in cells with TNF/CHX-induced apoptosis were compared to cells treated with TNF/CHX and transfected with a negative control siRNA (assumed as equal 1). In each case the value of caspase 3/7 activity after TNF/CHX treatment was divided by the value of basal activity of non-stimulated cells. Values represent the mean of three independent experiments with error bars showing standard error (* p

    Journal: BMC Cell Biology

    Article Title: Mimitin - a novel cytokine-regulated mitochondrial protein

    doi: 10.1186/1471-2121-10-23

    Figure Lengend Snippet: Effect of mimitin gene silencing on caspase 3/7 activities . HepG2 cells were transfected with mimitin-siRNA, GAPDH-siRNA, negative control siRNA, or transfection reagent (siPORT NeoFX) (blank). Apoptosis was induced 72 h post transfection by treatment of cells with TNF/CHX for 5 h. Caspase 3/7 activity was measured using Caspase-GloTM3/7 assay kit. A. Changes in caspase activity in cells with TNF/CHX-induced apoptosis were compared to cells treated with TNF/CHX and transfected with a negative control siRNA (assumed as equal 1). In each case the value of caspase 3/7 activity after TNF/CHX treatment was divided by the value of basal activity of non-stimulated cells. Values represent the mean of three independent experiments with error bars showing standard error (* p

    Article Snippet: Lipid-based transfection reagent siPORT NeoFX (4 μl/well) from Ambion and siRNAs (50 nM final concentration) were separately diluted in 50 μl OPTIMEM and then mixed together.

    Techniques: Transfection, Negative Control, Activity Assay

    Luciferase assay of paired box gene 6 (PAX6) promoter activity. A : Schematic indicates the representation of the PAX6 transcript unit in humans. The boxes denote exons. Braces indicate the extent of P0, P1, and Pα promoters in the PAX6 gene area. B : Luciferase assay of P0, P1, and Pα promoter activity in Y-79 cells. C : Luciferase assay of P0, P1, and Pα promoter activity in WERI cells. P0 of pGL4.10 insert indicates the insertion of P0 promoter sequences (5315 bp), P1 promoter activity (3796 bp), and Pα promoter sequences (3628 bp). White and black squares indicate the no-CN-A treatment and CN-A treatment, respectively. The negative pGL4.10 insert indicates an empty sequence. The data are presented after normalizing transfection efficiency using the Renilla luciferase reporter gene (n=3); the error bar indicates the standard deviation; Mann–Whitney U -test, *p

    Journal: Molecular Vision

    Article Title: Cotylenin A inhibits cell proliferation and induces apoptosis and PAX6 mRNA transcripts in retinoblastoma cell lines

    doi:

    Figure Lengend Snippet: Luciferase assay of paired box gene 6 (PAX6) promoter activity. A : Schematic indicates the representation of the PAX6 transcript unit in humans. The boxes denote exons. Braces indicate the extent of P0, P1, and Pα promoters in the PAX6 gene area. B : Luciferase assay of P0, P1, and Pα promoter activity in Y-79 cells. C : Luciferase assay of P0, P1, and Pα promoter activity in WERI cells. P0 of pGL4.10 insert indicates the insertion of P0 promoter sequences (5315 bp), P1 promoter activity (3796 bp), and Pα promoter sequences (3628 bp). White and black squares indicate the no-CN-A treatment and CN-A treatment, respectively. The negative pGL4.10 insert indicates an empty sequence. The data are presented after normalizing transfection efficiency using the Renilla luciferase reporter gene (n=3); the error bar indicates the standard deviation; Mann–Whitney U -test, *p

    Article Snippet: A lipid transfection system (siPORT NeoFX transfection agent; Ambion) was used to introduce siRNAs into Y-79 and WERI cells via the following protocol: cells were preincubated with or without 10 μg/ml CN-A for 24 h, after which they were resuspended at a density of 106 cells/ml growth medium.

    Techniques: Luciferase, Activity Assay, Sequencing, Transfection, Standard Deviation, MANN-WHITNEY

    Response of paired box gene 6 (PAX6) knockdown on rhodopsin ( RHO) expression inretinoblastoma cell lines. PAX6 siRNA was introduced into Y-79 and WERI cells via siPORT for knockdown of PAX6 mRNA. The electrophoresis photographs and the bar graphs indicate the result of reverse transcription polymerase chain reaction (RT–PCR) in Y-79 cells ( A ) and WERI cells ( B ). PAX6 and RHO mRNA expression were investigated using RT–PCR. The mRNA expression was detected 48 h after siRNA transfection and cotylenin A (CN-A) treatment. The bar graph indicates transduction of PAX6 , RHO , and GAPDH mRNA expression by PAX6 siRNA (n=3); the error bar indicates the standard deviation; Mann–Whitney U -test, *p

    Journal: Molecular Vision

    Article Title: Cotylenin A inhibits cell proliferation and induces apoptosis and PAX6 mRNA transcripts in retinoblastoma cell lines

    doi:

    Figure Lengend Snippet: Response of paired box gene 6 (PAX6) knockdown on rhodopsin ( RHO) expression inretinoblastoma cell lines. PAX6 siRNA was introduced into Y-79 and WERI cells via siPORT for knockdown of PAX6 mRNA. The electrophoresis photographs and the bar graphs indicate the result of reverse transcription polymerase chain reaction (RT–PCR) in Y-79 cells ( A ) and WERI cells ( B ). PAX6 and RHO mRNA expression were investigated using RT–PCR. The mRNA expression was detected 48 h after siRNA transfection and cotylenin A (CN-A) treatment. The bar graph indicates transduction of PAX6 , RHO , and GAPDH mRNA expression by PAX6 siRNA (n=3); the error bar indicates the standard deviation; Mann–Whitney U -test, *p

    Article Snippet: A lipid transfection system (siPORT NeoFX transfection agent; Ambion) was used to introduce siRNAs into Y-79 and WERI cells via the following protocol: cells were preincubated with or without 10 μg/ml CN-A for 24 h, after which they were resuspended at a density of 106 cells/ml growth medium.

    Techniques: Expressing, Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Transfection, Transduction, Standard Deviation, MANN-WHITNEY