singleplex test system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher singleplex test system
    Singleplex Test System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex test system/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex test system - by Bioz Stars, 2020-07
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    Generated:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    Chromatin Immunoprecipitation:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    In Vitro:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    Single-particle Tracking:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

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  • 93
    Thermo Fisher immunocap system
    Comparison of sIgE results obtained with the <t>ImmunoCAP</t> ISAC test with 8 extract-based ImmunoCAP singelplex tests (n = 101).
    Immunocap System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunocap system/product/Thermo Fisher
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    immunocap system - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher trioplex real time rt pcr assay
    Analytical performance comparison between <t>Trioplex</t> assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per <t>PCR</t> reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Trioplex Real Time Rt Pcr Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trioplex real time rt pcr assay/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    trioplex real time rt pcr assay - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    Thermo Fisher immunocap singleplex tests
    Analytical performance comparison between <t>Trioplex</t> assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per <t>PCR</t> reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Immunocap Singleplex Tests, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunocap singleplex tests/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunocap singleplex tests - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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    Comparison of sIgE results obtained with the ImmunoCAP ISAC test with 8 extract-based ImmunoCAP singelplex tests (n = 101).

    Journal: Allergo Journal International

    Article Title: Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

    doi: 10.1007/s40629-015-0046-4

    Figure Lengend Snippet: Comparison of sIgE results obtained with the ImmunoCAP ISAC test with 8 extract-based ImmunoCAP singelplex tests (n = 101).

    Article Snippet: IgE Analysis IgE analysis of serum samples was performed (A) traditional extract-based singleplex-testing with the ImmunoCAP system and (B) with the molecular multiplex test ImmunoCAP® ISAC 112 (both Thermo Fisher Scientific, Uppsala, Sweden).

    Techniques:

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: Standard curves were generated and tested with the Trioplex real-time RT-PCR assay on each qPCR instrument, ABI 7500 Fast Dx (ThermoFisher), and QuantStudio Dx (ThermoFisher).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation