singleplex taqman assays  (Thermo Fisher)


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    Structured Review

    Thermo Fisher singleplex taqman assays
    Singleplex Taqman Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex taqman assays/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex taqman assays - by Bioz Stars, 2020-04
    85/100 stars

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    Related Articles

    Synthesized:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: RNA was isolated and cDNA synthesized from ∼50,000 hEB-derived cells or undifferentiated hESCs using the Taqman Gene Expression Cells-to-CT kit (Ambion). cDNA was quantitated using a Nanodrop ND-1000 Spectrophotometer (Nanodrop Technologies, ND Software version 3.3.0). .. Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975).

    Isolation:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: For analysis of miRNA expression, miRNAs were isolated from hESCs or sorted cells using the mirVana miRNA Isolation kit (Ambion), and cDNA was reverse transcribed using the TaqMan MicroRNA RT kit (ABI). .. Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975).

    Spectrophotometry:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: RNA was isolated and cDNA synthesized from ∼50,000 hEB-derived cells or undifferentiated hESCs using the Taqman Gene Expression Cells-to-CT kit (Ambion). cDNA was quantitated using a Nanodrop ND-1000 Spectrophotometer (Nanodrop Technologies, ND Software version 3.3.0). .. Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975).

    Real-time Polymerase Chain Reaction:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: Paragraph title: Quantitative real-time PCR ... Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975).

    Expressing:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: .. Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975). ..

    Sequencing:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: Cycle times to detection were normalized against a reference gene, GAPDH, and relative changes were calculated using ABI Version 1.4 Sequence Detection Software. .. Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975).

    Software:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: Cycle times to detection were normalized against a reference gene, GAPDH, and relative changes were calculated using ABI Version 1.4 Sequence Detection Software. .. Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975).

    TaqMan Assay:

    Article Title: miR-125b Promotes Early Germ Layer Specification through Lin28/let-7d and Preferential Differentiation of Mesoderm in Human Embryonic Stem Cells
    Article Snippet: Relative expression was determined using the TaqMan Assay (Applied Biosystems) on an ABI 7300 Real-Time PCR system with the following primer pairs (ABI): Lin28 (Hs00702802_s1), GATA4 (Hs00171403_m1), Nkx2-5 (Hs00231763_m1), sarcolipin (Hs00161903_m1;Hs01888464_s1), MLC2v (Hs00166405_m1;Hs01125721_m1), αMHC (Hs00411908_m1), cTnT (Hs00165960_m1), α-fetoprotein (Hs00173490_m1), nestin (Hs00707120_s1), Cx43 (Hs00748445_s1), Cx40 (Hs00979198_m1), Cx45 (Hs00271416_s1), and GAPDH (4326317E). .. Following linear pre-amplification of miRNA sequences using the Applied Biosystems PreAmp system, relative expression was determined using singleplex TaqMan Assays (Applied Biosystems) with primer sets for human miR-125b (Applied Biosystems; 000449) and let-7d (Applied Biosystems; 4427975).

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    Thermo Fisher taqman qpcr taqman singleplex
    Multiplex to <t>singleplex</t> <t>qPCR</t> Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P
    Taqman Qpcr Taqman Singleplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman qpcr taqman singleplex/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taqman qpcr taqman singleplex - by Bioz Stars, 2020-04
    94/100 stars
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    91
    Thermo Fisher singleplex taqman pcr
    Multiplex to <t>singleplex</t> <t>qPCR</t> Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P
    Singleplex Taqman Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex taqman pcr/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex taqman pcr - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    Multiplex to singleplex qPCR Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P

    Journal: BMC Biotechnology

    Article Title: Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection

    doi: 10.1186/s12896-019-0571-1

    Figure Lengend Snippet: Multiplex to singleplex qPCR Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P

    Article Snippet: TaqMan qPCR TaqMan singleplex and multiplex qPCR runs were performed on a QuantStudio 6 Flex instrument (Thermo Fisher Scientific) equipped with a 384-well block.

    Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction, Singleplex Assay, Plasmid Preparation, Concentration Assay