singleplex qpcr  (Thermo Fisher)


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    Structured Review

    Thermo Fisher singleplex qpcr
    Singleplex Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex qpcr/product/Thermo Fisher
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex qpcr - by Bioz Stars, 2020-02
    77/100 stars

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    Related Articles

    Transfection:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: RNA was isolated 24 hours post transfection using the MagMax 96 Total RNA isolation kit (Ambion), and cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions.

    Amplification:

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C). ..

    Synthesized:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: RNA was isolated 24 hours post transfection using the MagMax 96 Total RNA isolation kit (Ambion), and cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions.

    Isolation:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: RNA was isolated 24 hours post transfection using the MagMax 96 Total RNA isolation kit (Ambion), and cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions.

    Size-exclusion Chromatography:

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C). ..

    Labeling:

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Forty‐five nanograms of cDNA was amplified using Taqman® reagents and predesigned gene expression assays FAM® labeled probes (Life Technologies Corp, NY, USA) for p62 (Mm00448091_m1) and LC3 (Mm00458724_m1) with 18s (#4318839, VIC® labeled) as reference gene. .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

    Real-time Polymerase Chain Reaction:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions. .. GAPDH was used as a reference gene, and the relative expression levels of target mRNA were normalized against cells transfected with Allstars non-targeting control siRNA.

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C). ..

    Concentration Assay:

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Total mRNA from mouse quadriceps muscle using an RNeasy kit (Qiagen Inc, Valencia, CA, USA) then purity and concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., DE, USA). .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

    Spectrophotometry:

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Total mRNA from mouse quadriceps muscle using an RNeasy kit (Qiagen Inc, Valencia, CA, USA) then purity and concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., DE, USA). .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

    Expressing:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions. .. GAPDH was used as a reference gene, and the relative expression levels of target mRNA were normalized against cells transfected with Allstars non-targeting control siRNA.

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Forty‐five nanograms of cDNA was amplified using Taqman® reagents and predesigned gene expression assays FAM® labeled probes (Life Technologies Corp, NY, USA) for p62 (Mm00448091_m1) and LC3 (Mm00458724_m1) with 18s (#4318839, VIC® labeled) as reference gene. .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

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    Thermo Fisher taqman qpcr taqman singleplex
    Multiplex to <t>singleplex</t> <t>qPCR</t> Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P
    Taqman Qpcr Taqman Singleplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman qpcr taqman singleplex/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taqman qpcr taqman singleplex - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Thermo Fisher singleplex qpcr
    Multiplex to <t>singleplex</t> <t>qPCR</t> Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P
    Singleplex Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex qpcr/product/Thermo Fisher
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex qpcr - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    Image Search Results


    Multiplex to singleplex qPCR Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P

    Journal: BMC Biotechnology

    Article Title: Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection

    doi: 10.1186/s12896-019-0571-1

    Figure Lengend Snippet: Multiplex to singleplex qPCR Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P

    Article Snippet: TaqMan qPCR TaqMan singleplex and multiplex qPCR runs were performed on a QuantStudio 6 Flex instrument (Thermo Fisher Scientific) equipped with a 384-well block.

    Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction, Singleplex Assay, Plasmid Preparation, Concentration Assay