singleplex qpcr  (Thermo Fisher)


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    Structured Review

    Thermo Fisher singleplex qpcr
    Singleplex Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex qpcr/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex qpcr - by Bioz Stars, 2020-04
    85/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions. .. GAPDH was used as a reference gene, and the relative expression levels of target mRNA were normalized against cells transfected with Allstars non-targeting control siRNA.

    Article Title: Genetic Variation of Bordetella pertussis in Austria
    Article Snippet: .. Singleplex qPCR for detecting the target SNPs in ptxA , ptxB , fimD , tcfA and bvgS genes was performed in a 15 μl reaction in 96-well plates using the StepOnePlus Real Time PCR System running under software version 2.0 (Life Technologies). .. The reaction contained 1 × PCR buffer B2 (Solis Biodyne, Tartu, Estonia), 4.5 mM MgCl2 , 0.2 mM of each dNTP, 250 nM consensus primer, 150 nM ARMS primer, 150 nM hydrolysis probe, 1 U HOT FIREPol DNA Polymerase (5 U/μl, Solis Biodyne), and 8 ng DNA.

    Article Title: Nanostructural and Transcriptomic Analyses of Human Saliva Derived Exosomes
    Article Snippet: .. Quantitative PCR Each transcript was quantified from 2 µL aliquots of preamplified samples via a singleplex qPCR in an SDS 7500 Fast instrument (Applied Biosystems, Foster City, CA) with a 10-µL reaction volume containing 300 nmol/L of each of the inner primers and the SYBR Green Power Master Mix (Applied Biosystems). .. After 10 min of polymerase activation at 95°C, we carried out 40 cycles of 15 s at 95°C and 60 s at 60°C and then performed a melting curve analysis. shows the primers sets used for this study (supplementary methods).

    Article Title: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
    Article Snippet: .. Reverse transcription and singleplex qPCR based on SYBR Green chemistry As a reference method to measure gene expression, singleplex qPCR assays based on SYBR Green chemistry were performed. cDNA was synthesized using 1 μg οf total RNA, previously treated with TURBO™ DNase (Invitrogen, Carlsbad, CA, USA), with oligo (dT)12-18 primers and the Thermoscript RT-PCR system kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. The SYBR Green-based qPCR assays were run in duplicates in 10 μl reactions, consisting of 2× Kapa SYBR® Fast Universal qPCR Master Mix (Kapa Biosystems, Wilmington, MA, USA), forward and reverse primers specific for each gene (Additional file : Table S2) at a final concentration of 200 nM as well as 20 ng of cDNA template.

    Article Title: Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma
    Article Snippet: .. Singleplex qPCR was performed after first strand cDNA synthesis using 2× Taqman Universal PCR Master Mix (Applied Biosystems, Inc., Cat#4364338) and Amplitaq Gold DNA polymerase, LD (Applied Biosystems, Inc., Cat#4338857) and specific primer/probe sets (Applied Biosystems, Inc.). .. Five cases were tested with commercially available optimized primer/probe sets for MMP-3 [TaqMan Gene Expression Assays, Inventoried Assay ID: Hs00233962 for MMP-3 (stromelysin-1, progelatinase), Applied BioSystems, Inc., Cat.# 4331182] and MMP-10 [TaqMan Gene Expression Assays, Inventoried Assay ID: Hs00233987 for MMP-10 (stromelysin-2), Applied BioSystems, Inc., Cat.# 4331182] gene expression levels.

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C). ..

    Article Title: Exon Level Expression Profiling: a Comprehensive Transcriptome Analysis for Oral Fluids
    Article Snippet: .. From 2 μL aliquots of pre-amplified samples, each transcript was quantified by singleplex qPCR in 10 μL reactions with the inner primers at 300 nM each, using the SYBR Green Power mix (Applied Biosystems), in an SDS 7500 Fast instrument (Applied Biosystems). .. After 10 min polymerase activation at 95 °C, 40 cycles of 15 s at 95 °C and 60 s at 60 °C were performed, followed by melting curve analysis.

    Transfection:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: RNA was isolated 24 hours post transfection using the MagMax 96 Total RNA isolation kit (Ambion), and cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions.

    Amplification:

    Article Title: Genetic Variation of Bordetella pertussis in Austria
    Article Snippet: Paragraph title: Amplification refractory mutation system quantitative PCR (ARMS-qPCR) to detect alleles of ptxA , ptxB , fimD , tcfA and bvgS genes as well as the ptxP3 and ptxP1-like alleles ... Singleplex qPCR for detecting the target SNPs in ptxA , ptxB , fimD , tcfA and bvgS genes was performed in a 15 μl reaction in 96-well plates using the StepOnePlus Real Time PCR System running under software version 2.0 (Life Technologies).

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C). ..

    Synthesized:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: RNA was isolated 24 hours post transfection using the MagMax 96 Total RNA isolation kit (Ambion), and cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions.

    Article Title: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
    Article Snippet: .. Reverse transcription and singleplex qPCR based on SYBR Green chemistry As a reference method to measure gene expression, singleplex qPCR assays based on SYBR Green chemistry were performed. cDNA was synthesized using 1 μg οf total RNA, previously treated with TURBO™ DNase (Invitrogen, Carlsbad, CA, USA), with oligo (dT)12-18 primers and the Thermoscript RT-PCR system kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. The SYBR Green-based qPCR assays were run in duplicates in 10 μl reactions, consisting of 2× Kapa SYBR® Fast Universal qPCR Master Mix (Kapa Biosystems, Wilmington, MA, USA), forward and reverse primers specific for each gene (Additional file : Table S2) at a final concentration of 200 nM as well as 20 ng of cDNA template.

    Mutagenesis:

    Article Title: Genetic Variation of Bordetella pertussis in Austria
    Article Snippet: Paragraph title: Amplification refractory mutation system quantitative PCR (ARMS-qPCR) to detect alleles of ptxA , ptxB , fimD , tcfA and bvgS genes as well as the ptxP3 and ptxP1-like alleles ... Singleplex qPCR for detecting the target SNPs in ptxA , ptxB , fimD , tcfA and bvgS genes was performed in a 15 μl reaction in 96-well plates using the StepOnePlus Real Time PCR System running under software version 2.0 (Life Technologies).

    Isolation:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: RNA was isolated 24 hours post transfection using the MagMax 96 Total RNA isolation kit (Ambion), and cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions.

    Article Title: Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma
    Article Snippet: Quantitative RT-PCR Total RNA was isolated with the PicoPure RNA Isolation kit (Arcturus Engineering) as suggested by the manufacturer. .. Singleplex qPCR was performed after first strand cDNA synthesis using 2× Taqman Universal PCR Master Mix (Applied Biosystems, Inc., Cat#4364338) and Amplitaq Gold DNA polymerase, LD (Applied Biosystems, Inc., Cat#4338857) and specific primer/probe sets (Applied Biosystems, Inc.).

    Size-exclusion Chromatography:

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C). ..

    Quantitative RT-PCR:

    Article Title: Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: Quantitative RT-PCR ... Singleplex qPCR was performed after first strand cDNA synthesis using 2× Taqman Universal PCR Master Mix (Applied Biosystems, Inc., Cat#4364338) and Amplitaq Gold DNA polymerase, LD (Applied Biosystems, Inc., Cat#4338857) and specific primer/probe sets (Applied Biosystems, Inc.).

    Concentration Assay:

    Article Title: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
    Article Snippet: Reverse transcription and singleplex qPCR based on SYBR Green chemistry As a reference method to measure gene expression, singleplex qPCR assays based on SYBR Green chemistry were performed. cDNA was synthesized using 1 μg οf total RNA, previously treated with TURBO™ DNase (Invitrogen, Carlsbad, CA, USA), with oligo (dT)12-18 primers and the Thermoscript RT-PCR system kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. The SYBR Green-based qPCR assays were run in duplicates in 10 μl reactions, consisting of 2× Kapa SYBR® Fast Universal qPCR Master Mix (Kapa Biosystems, Wilmington, MA, USA), forward and reverse primers specific for each gene (Additional file : Table S2) at a final concentration of 200 nM as well as 20 ng of cDNA template.

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Total mRNA from mouse quadriceps muscle using an RNeasy kit (Qiagen Inc, Valencia, CA, USA) then purity and concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., DE, USA). .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

    SYBR Green Assay:

    Article Title: Nanostructural and Transcriptomic Analyses of Human Saliva Derived Exosomes
    Article Snippet: .. Quantitative PCR Each transcript was quantified from 2 µL aliquots of preamplified samples via a singleplex qPCR in an SDS 7500 Fast instrument (Applied Biosystems, Foster City, CA) with a 10-µL reaction volume containing 300 nmol/L of each of the inner primers and the SYBR Green Power Master Mix (Applied Biosystems). .. After 10 min of polymerase activation at 95°C, we carried out 40 cycles of 15 s at 95°C and 60 s at 60°C and then performed a melting curve analysis. shows the primers sets used for this study (supplementary methods).

    Article Title: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
    Article Snippet: .. Reverse transcription and singleplex qPCR based on SYBR Green chemistry As a reference method to measure gene expression, singleplex qPCR assays based on SYBR Green chemistry were performed. cDNA was synthesized using 1 μg οf total RNA, previously treated with TURBO™ DNase (Invitrogen, Carlsbad, CA, USA), with oligo (dT)12-18 primers and the Thermoscript RT-PCR system kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. The SYBR Green-based qPCR assays were run in duplicates in 10 μl reactions, consisting of 2× Kapa SYBR® Fast Universal qPCR Master Mix (Kapa Biosystems, Wilmington, MA, USA), forward and reverse primers specific for each gene (Additional file : Table S2) at a final concentration of 200 nM as well as 20 ng of cDNA template.

    Article Title: Exon Level Expression Profiling: a Comprehensive Transcriptome Analysis for Oral Fluids
    Article Snippet: .. From 2 μL aliquots of pre-amplified samples, each transcript was quantified by singleplex qPCR in 10 μL reactions with the inner primers at 300 nM each, using the SYBR Green Power mix (Applied Biosystems), in an SDS 7500 Fast instrument (Applied Biosystems). .. After 10 min polymerase activation at 95 °C, 40 cycles of 15 s at 95 °C and 60 s at 60 °C were performed, followed by melting curve analysis.

    Activation Assay:

    Article Title: Nanostructural and Transcriptomic Analyses of Human Saliva Derived Exosomes
    Article Snippet: Quantitative PCR Each transcript was quantified from 2 µL aliquots of preamplified samples via a singleplex qPCR in an SDS 7500 Fast instrument (Applied Biosystems, Foster City, CA) with a 10-µL reaction volume containing 300 nmol/L of each of the inner primers and the SYBR Green Power Master Mix (Applied Biosystems). .. After 10 min of polymerase activation at 95°C, we carried out 40 cycles of 15 s at 95°C and 60 s at 60°C and then performed a melting curve analysis. shows the primers sets used for this study (supplementary methods).

    Article Title: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
    Article Snippet: Reverse transcription and singleplex qPCR based on SYBR Green chemistry As a reference method to measure gene expression, singleplex qPCR assays based on SYBR Green chemistry were performed. cDNA was synthesized using 1 μg οf total RNA, previously treated with TURBO™ DNase (Invitrogen, Carlsbad, CA, USA), with oligo (dT)12-18 primers and the Thermoscript RT-PCR system kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. Bio-Rad CFX Connect™ Real-Time PCR Detection was used with a thermal protocol consisting of a 3 min polymerase activation/initial denaturation step at 95 °C, 40 cycles of denaturation and annealing/extension steps at 95 °C for 3 s, 60 °C for 30 s, followed by a melting curve analysis step.

    Article Title: Exon Level Expression Profiling: a Comprehensive Transcriptome Analysis for Oral Fluids
    Article Snippet: From 2 μL aliquots of pre-amplified samples, each transcript was quantified by singleplex qPCR in 10 μL reactions with the inner primers at 300 nM each, using the SYBR Green Power mix (Applied Biosystems), in an SDS 7500 Fast instrument (Applied Biosystems). .. After 10 min polymerase activation at 95 °C, 40 cycles of 15 s at 95 °C and 60 s at 60 °C were performed, followed by melting curve analysis.

    Polymerase Chain Reaction:

    Article Title: Genetic Variation of Bordetella pertussis in Austria
    Article Snippet: Singleplex qPCR for detecting the target SNPs in ptxA , ptxB , fimD , tcfA and bvgS genes was performed in a 15 μl reaction in 96-well plates using the StepOnePlus Real Time PCR System running under software version 2.0 (Life Technologies). .. The reaction contained 1 × PCR buffer B2 (Solis Biodyne, Tartu, Estonia), 4.5 mM MgCl2 , 0.2 mM of each dNTP, 250 nM consensus primer, 150 nM ARMS primer, 150 nM hydrolysis probe, 1 U HOT FIREPol DNA Polymerase (5 U/μl, Solis Biodyne), and 8 ng DNA.

    Article Title: Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma
    Article Snippet: .. Singleplex qPCR was performed after first strand cDNA synthesis using 2× Taqman Universal PCR Master Mix (Applied Biosystems, Inc., Cat#4364338) and Amplitaq Gold DNA polymerase, LD (Applied Biosystems, Inc., Cat#4338857) and specific primer/probe sets (Applied Biosystems, Inc.). .. Five cases were tested with commercially available optimized primer/probe sets for MMP-3 [TaqMan Gene Expression Assays, Inventoried Assay ID: Hs00233962 for MMP-3 (stromelysin-1, progelatinase), Applied BioSystems, Inc., Cat.# 4331182] and MMP-10 [TaqMan Gene Expression Assays, Inventoried Assay ID: Hs00233987 for MMP-10 (stromelysin-2), Applied BioSystems, Inc., Cat.# 4331182] gene expression levels.

    Spectrophotometry:

    Article Title: Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma
    Article Snippet: RNA quantity was assessed using NanoDrop Spectrophotometer (NanoDrop Technologies, Wilmington, DE). .. Singleplex qPCR was performed after first strand cDNA synthesis using 2× Taqman Universal PCR Master Mix (Applied Biosystems, Inc., Cat#4364338) and Amplitaq Gold DNA polymerase, LD (Applied Biosystems, Inc., Cat#4338857) and specific primer/probe sets (Applied Biosystems, Inc.).

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Total mRNA from mouse quadriceps muscle using an RNeasy kit (Qiagen Inc, Valencia, CA, USA) then purity and concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., DE, USA). .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

    Expressing:

    Article Title: Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
    Article Snippet: .. The relative amount of target mRNA was determined by singleplex quantitative PCR using pre-designed Taqman gene expression assays and Taqman Fast Advanced Master Mix (Applied Biosystems) following the manufacturer’s instructions. .. GAPDH was used as a reference gene, and the relative expression levels of target mRNA were normalized against cells transfected with Allstars non-targeting control siRNA.

    Article Title: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
    Article Snippet: .. Reverse transcription and singleplex qPCR based on SYBR Green chemistry As a reference method to measure gene expression, singleplex qPCR assays based on SYBR Green chemistry were performed. cDNA was synthesized using 1 μg οf total RNA, previously treated with TURBO™ DNase (Invitrogen, Carlsbad, CA, USA), with oligo (dT)12-18 primers and the Thermoscript RT-PCR system kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. The SYBR Green-based qPCR assays were run in duplicates in 10 μl reactions, consisting of 2× Kapa SYBR® Fast Universal qPCR Master Mix (Kapa Biosystems, Wilmington, MA, USA), forward and reverse primers specific for each gene (Additional file : Table S2) at a final concentration of 200 nM as well as 20 ng of cDNA template.

    Article Title: Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma
    Article Snippet: Total RNA was used to generate complementary DNA (cDNA) using the Taqman High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Inc., Foster City, CA, USA Cat # 4374966) as suggested by the manufacturer to get the maximum expression of transcripts. .. Singleplex qPCR was performed after first strand cDNA synthesis using 2× Taqman Universal PCR Master Mix (Applied Biosystems, Inc., Cat#4364338) and Amplitaq Gold DNA polymerase, LD (Applied Biosystems, Inc., Cat#4338857) and specific primer/probe sets (Applied Biosystems, Inc.).

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Forty‐five nanograms of cDNA was amplified using Taqman® reagents and predesigned gene expression assays FAM® labeled probes (Life Technologies Corp, NY, USA) for p62 (Mm00448091_m1) and LC3 (Mm00458724_m1) with 18s (#4318839, VIC® labeled) as reference gene. .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
    Article Snippet: .. Reverse transcription and singleplex qPCR based on SYBR Green chemistry As a reference method to measure gene expression, singleplex qPCR assays based on SYBR Green chemistry were performed. cDNA was synthesized using 1 μg οf total RNA, previously treated with TURBO™ DNase (Invitrogen, Carlsbad, CA, USA), with oligo (dT)12-18 primers and the Thermoscript RT-PCR system kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. The SYBR Green-based qPCR assays were run in duplicates in 10 μl reactions, consisting of 2× Kapa SYBR® Fast Universal qPCR Master Mix (Kapa Biosystems, Wilmington, MA, USA), forward and reverse primers specific for each gene (Additional file : Table S2) at a final concentration of 200 nM as well as 20 ng of cDNA template.

    Labeling:

    Article Title: Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice. Skeletal muscle autophagy remains responsive to hyperinsulinemia and hyperglycemia at higher plasma insulin concentrations in insulin‐resistant mice
    Article Snippet: Forty‐five nanograms of cDNA was amplified using Taqman® reagents and predesigned gene expression assays FAM® labeled probes (Life Technologies Corp, NY, USA) for p62 (Mm00448091_m1) and LC3 (Mm00458724_m1) with 18s (#4318839, VIC® labeled) as reference gene. .. Singleplex qPCR (ABI 7900, Thermo Fisher Scientific Inc., DE, USA) was performed with target and reference genes amplified in 20 μ L on 384 well clear plates with 40 cycles of 15 sec denaturing (95°C) and 60 sec annealing/extension (60°C).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Increased matrix metalloproteinase activation in esophageal squamous cell carcinoma
    Article Snippet: .. Singleplex qPCR was performed after first strand cDNA synthesis using 2× Taqman Universal PCR Master Mix (Applied Biosystems, Inc., Cat4364338) and Amplitaq Gold DNA polymerase, LD (Applied Biosystems, Inc., Cat#4338857) and specific primer/probe sets (Applied Biosystems, Inc.). .. Five cases were tested with commercially available optimized primer/probe sets for MMP-3 [TaqMan Gene Expression Assays, Inventoried Assay ID: Hs00233962 for MMP-3 (stromelysin-1, progelatinase), Applied BioSystems, Inc., Cat.# 4331182] and MMP-10 [TaqMan Gene Expression Assays, Inventoried Assay ID: Hs00233987 for MMP-10 (stromelysin-2), Applied BioSystems, Inc., Cat.# 4331182] gene expression levels.

    Software:

    Article Title: Genetic Variation of Bordetella pertussis in Austria
    Article Snippet: .. Singleplex qPCR for detecting the target SNPs in ptxA , ptxB , fimD , tcfA and bvgS genes was performed in a 15 μl reaction in 96-well plates using the StepOnePlus Real Time PCR System running under software version 2.0 (Life Technologies). .. The reaction contained 1 × PCR buffer B2 (Solis Biodyne, Tartu, Estonia), 4.5 mM MgCl2 , 0.2 mM of each dNTP, 250 nM consensus primer, 150 nM ARMS primer, 150 nM hydrolysis probe, 1 U HOT FIREPol DNA Polymerase (5 U/μl, Solis Biodyne), and 8 ng DNA.

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  • 90
    Thermo Fisher quantstudio dx
    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the <t>QuantStudio</t> Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)
    Quantstudio Dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantstudio dx/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    quantstudio dx - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    94
    Thermo Fisher taqman qpcr taqman singleplex
    Multiplex to <t>singleplex</t> <t>qPCR</t> Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P
    Taqman Qpcr Taqman Singleplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman qpcr taqman singleplex/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taqman qpcr taqman singleplex - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    85
    Thermo Fisher singleplex qpcr
    Multiplex to <t>singleplex</t> <t>qPCR</t> Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P
    Singleplex Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex qpcr/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex qpcr - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results


    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the QuantStudio Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the QuantStudio Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: RNA Extraction, Standard Deviation

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in whole blood (EDTA). A pool of whole blood donated by healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD) and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicas of every dilution were extracted using small volume protocol (0.2 mL) and tested with Trioplex assay on the ABI7500 Fast Dx instrument ( a ) or in the QuantStudio Dx instrument ( b ). Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in whole blood (EDTA). A pool of whole blood donated by healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD) and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicas of every dilution were extracted using small volume protocol (0.2 mL) and tested with Trioplex assay on the ABI7500 Fast Dx instrument ( a ) or in the QuantStudio Dx instrument ( b ). Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: RNA Extraction, Standard Deviation

    Multiplex to singleplex qPCR Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P

    Journal: BMC Biotechnology

    Article Title: Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection

    doi: 10.1186/s12896-019-0571-1

    Figure Lengend Snippet: Multiplex to singleplex qPCR Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. ( N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P

    Article Snippet: TaqMan qPCR TaqMan singleplex and multiplex qPCR runs were performed on a QuantStudio 6 Flex instrument (Thermo Fisher Scientific) equipped with a 384-well block.

    Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction, Singleplex Assay, Plasmid Preparation, Concentration Assay