single tube pcr kit  (TaKaRa)

 
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    Name:
    LA PCR Kit
    Description:
    The LA PCR Kit Version 2 1 contains all the reagents needed for amplification of long DNA templates enabling routine amplification of products up to 20 kb in length including GC rich amplicons For some DNA templates amplification of up to 48 kb is possible This long range PCR kit contains TaKaRa LA Taq DNA Polymerase buffers MgCl2 dNTPs molecular weight markers and control templates plus corresponding primers to ensure optimal PCR performance during long PCR
    Catalog Number:
    rr013b
    Price:
    None
    Size:
    100 Rxns
    Category:
    LA Taq PCR kit LA Taq products Long range PCR PCR
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    Structured Review

    TaKaRa single tube pcr kit
    Detection of <t>HTLV-1</t> proviruses integrated in FPM1 cells by Southern blot hybridization (a) and <t>long-PCR</t> (b) methods. In panel a, 20 μg of DNA extracted from MT-2 (lane 1) and FPM1 cells (lane 2) was digested with Eco RI and hybridized with radiolabeled HTLV-1-specific probe. In panel b, 500 ng of DNA from MOLT-4 (lane 1), TL-OmI (lane 2), FPM1 (lane 3), and MT-2 (lane 4) were subjected to long PCR with primers for 5′ and 3′ HTLV-1 LTR. The expected size of the PCR products from a full-length HTLV-1 provirus is 7.7 kbp.
    The LA PCR Kit Version 2 1 contains all the reagents needed for amplification of long DNA templates enabling routine amplification of products up to 20 kb in length including GC rich amplicons For some DNA templates amplification of up to 48 kb is possible This long range PCR kit contains TaKaRa LA Taq DNA Polymerase buffers MgCl2 dNTPs molecular weight markers and control templates plus corresponding primers to ensure optimal PCR performance during long PCR
    https://www.bioz.com/result/single tube pcr kit/product/TaKaRa
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    single tube pcr kit - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    nested pcr
    htlv-1 provirus

    Images

    1) Product Images from "Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax"

    Article Title: Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax

    Journal: Journal of Virology

    doi:

    Detection of HTLV-1 proviruses integrated in FPM1 cells by Southern blot hybridization (a) and long-PCR (b) methods. In panel a, 20 μg of DNA extracted from MT-2 (lane 1) and FPM1 cells (lane 2) was digested with Eco RI and hybridized with radiolabeled HTLV-1-specific probe. In panel b, 500 ng of DNA from MOLT-4 (lane 1), TL-OmI (lane 2), FPM1 (lane 3), and MT-2 (lane 4) were subjected to long PCR with primers for 5′ and 3′ HTLV-1 LTR. The expected size of the PCR products from a full-length HTLV-1 provirus is 7.7 kbp.
    Figure Legend Snippet: Detection of HTLV-1 proviruses integrated in FPM1 cells by Southern blot hybridization (a) and long-PCR (b) methods. In panel a, 20 μg of DNA extracted from MT-2 (lane 1) and FPM1 cells (lane 2) was digested with Eco RI and hybridized with radiolabeled HTLV-1-specific probe. In panel b, 500 ng of DNA from MOLT-4 (lane 1), TL-OmI (lane 2), FPM1 (lane 3), and MT-2 (lane 4) were subjected to long PCR with primers for 5′ and 3′ HTLV-1 LTR. The expected size of the PCR products from a full-length HTLV-1 provirus is 7.7 kbp.

    Techniques Used: Southern Blot, Hybridization, Polymerase Chain Reaction

    RT-PCR analysis of HTLV-1 mRNA. A total of 300 ng of DNase-treated total RNA from MT-2 (a) or FPM1 (b) was subjected to 30 cycles of one-step RT-PCR with primers for G3PDH (lane 1), gag (lane 2), env (lane 3), and pX (lane 4). The expected size of the amplified products was 435 bp for G3PDH, 242 bp for HTLV-1 gag , 316 bp for env , and 145 bp for pX.
    Figure Legend Snippet: RT-PCR analysis of HTLV-1 mRNA. A total of 300 ng of DNase-treated total RNA from MT-2 (a) or FPM1 (b) was subjected to 30 cycles of one-step RT-PCR with primers for G3PDH (lane 1), gag (lane 2), env (lane 3), and pX (lane 4). The expected size of the amplified products was 435 bp for G3PDH, 242 bp for HTLV-1 gag , 316 bp for env , and 145 bp for pX.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Amplification

    Identification of HTLV-1 flanking region of FPM1. A 1-μg portion of DNA was extracted from the peripheral blood cells of a F344/N rat inoculated with MT-2 cells (lane 1) and two rats inoculated with FPM1 cells (lanes 2 and 3) 37 weeks after inoculation. The DNA was subjected to PCR with primers specific for G3PDH (top), HTLV-1 pX (middle), and HTLV-1 flanking region of FPM1 (bottom).
    Figure Legend Snippet: Identification of HTLV-1 flanking region of FPM1. A 1-μg portion of DNA was extracted from the peripheral blood cells of a F344/N rat inoculated with MT-2 cells (lane 1) and two rats inoculated with FPM1 cells (lanes 2 and 3) 37 weeks after inoculation. The DNA was subjected to PCR with primers specific for G3PDH (top), HTLV-1 pX (middle), and HTLV-1 flanking region of FPM1 (bottom).

    Techniques Used: Polymerase Chain Reaction

    Related Articles

    Amplification:

    Article Title: Murine lymph node-derived stromal cells effectively support survival but induce no activation/proliferation of peripheral resting T cells in vitro
    Article Snippet: .. Single-stranded cDNA was synthesized by reverse transcriptase (RT) with random hexamer oligonucleotides as primers, and the desired cDNA was amplified by polymerase chain reaction (PCR) using a TaKaRa RNA LA PCR kit (TaKaRa Biomedicals, Kyoto, Japan). .. Primers (sense and antisense) for the amplification of each cytokine cDNA were as follows: β-actin sense, TGG AAT CCT GTG GCA TCC ATG AAA C; β-actin antisense, TAA AAC GCA GCT CAG TAA CAG TCC G; IL-1α sense, CTC TAG AGC ACC ATG CTA CAG AC; IL-1α antisense, TGG AAT CCA GGG GAA ACA CTG; IL-2 sense, TGA TGG ACC TAC AGG AGC TCC TGA G; IL-2 antisense, GAG TCA AAT CCA GAA CAT GCC GCA G; IL-3 sense, GAA GTG GAT CCT GAG GAC AGA TAC G; IL-3 antisense, GAC CAT GGG CCA TGA GGA ACA TTC; IL-4 sense, CGA AGA ACA CCA CAG AGA GTG AGC T; IL-4 antisense, GAC TCA TTC ATG GTG CAG CTT ATC G; IL-5 sense, CTC TAG TAA GCC CAC TTC TA; IL-5 antisense, TGA TAC ATG AAT AAC ATC CC; IL-6 sense, TGG AGT CAC AGA AGG AGT GGC TAA G; IL-6 antisense, TCT GAC CAC AGA AGG AGT GGC TAA G; IL-7 sense, AAA TGC AGC TGA CTG CTG GC; IL-7 antisense, TCT CCA GTC TAA AAC AGG AC; IL-10 sense, TAC CTG GTA GAA GTG ATG CC; IL-10 antisense, CAT CAT GTA TGC TTC TAT GC; tumour necrosis factor-α (TNF-α) sense, GGC AGG TCT ACT TTG GAG TCA TTG C; TNF-α antisense, ACA TTC GAG GCT CCA GTG AAT TCG G; lymphotoxin (LT)-α sense, TGG CTG GGA ACA GGG GAA GGT TGA C; LT-α antisense, CGT GCT TTC TTC TAG AAC CCC TTG G. The conditions for PCR were 1 min at 94°, 2 min at 55° and 3 min at 72° for 30 cycles.

    Article Title: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NF?B pathways which enhances transcription of MnSOD by accelerating I?B? degradation in normal and cancer cells
    Article Snippet: .. RT-PCR Total cellular RNAs (1.0 μg) isolated with RNAiso Plus were used for cDNA preparation and then amplified by PCR kit (Takara Inc., Japan); First strand cDNA was synthesized using oligo-dT by reverse transcription reaction in 10 μl of reaction volume. .. The gene of interest was amplified by ExTaq polymerase in PCR kits using primer sequences described in the Additional file .

    Article Title: Tomato yellow leaf curl virus intergenic siRNAs target a host long noncoding RNA to modulate disease symptoms
    Article Snippet: .. The 5’ flanking region of the sense transcripts of SlLNR1 were obtained by RNA ligase-mediated rapid amplification of 5' cDNA ends First Choice ® RLM-RACE Kit (Invitrogen, USA), according to the instructions of the manufacturer, and the 3’ end was verified by 3’ RACE PCR kit (TAKARA). .. Full sense transcript of the SlLNR1 was amplified with the primers designed according to the joint sequence by RT-PCR.

    Article Title: Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation
    Article Snippet: .. The sequence of the gene coding the gametocyte protein was amplified by reverse transcription polymerase chain reaction (RT-PCR) using the RNA LA PCR Kit (TaKaRa Bio. ..

    Synthesized:

    Article Title: Murine lymph node-derived stromal cells effectively support survival but induce no activation/proliferation of peripheral resting T cells in vitro
    Article Snippet: .. Single-stranded cDNA was synthesized by reverse transcriptase (RT) with random hexamer oligonucleotides as primers, and the desired cDNA was amplified by polymerase chain reaction (PCR) using a TaKaRa RNA LA PCR kit (TaKaRa Biomedicals, Kyoto, Japan). .. Primers (sense and antisense) for the amplification of each cytokine cDNA were as follows: β-actin sense, TGG AAT CCT GTG GCA TCC ATG AAA C; β-actin antisense, TAA AAC GCA GCT CAG TAA CAG TCC G; IL-1α sense, CTC TAG AGC ACC ATG CTA CAG AC; IL-1α antisense, TGG AAT CCA GGG GAA ACA CTG; IL-2 sense, TGA TGG ACC TAC AGG AGC TCC TGA G; IL-2 antisense, GAG TCA AAT CCA GAA CAT GCC GCA G; IL-3 sense, GAA GTG GAT CCT GAG GAC AGA TAC G; IL-3 antisense, GAC CAT GGG CCA TGA GGA ACA TTC; IL-4 sense, CGA AGA ACA CCA CAG AGA GTG AGC T; IL-4 antisense, GAC TCA TTC ATG GTG CAG CTT ATC G; IL-5 sense, CTC TAG TAA GCC CAC TTC TA; IL-5 antisense, TGA TAC ATG AAT AAC ATC CC; IL-6 sense, TGG AGT CAC AGA AGG AGT GGC TAA G; IL-6 antisense, TCT GAC CAC AGA AGG AGT GGC TAA G; IL-7 sense, AAA TGC AGC TGA CTG CTG GC; IL-7 antisense, TCT CCA GTC TAA AAC AGG AC; IL-10 sense, TAC CTG GTA GAA GTG ATG CC; IL-10 antisense, CAT CAT GTA TGC TTC TAT GC; tumour necrosis factor-α (TNF-α) sense, GGC AGG TCT ACT TTG GAG TCA TTG C; TNF-α antisense, ACA TTC GAG GCT CCA GTG AAT TCG G; lymphotoxin (LT)-α sense, TGG CTG GGA ACA GGG GAA GGT TGA C; LT-α antisense, CGT GCT TTC TTC TAG AAC CCC TTG G. The conditions for PCR were 1 min at 94°, 2 min at 55° and 3 min at 72° for 30 cycles.

    Article Title: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NF?B pathways which enhances transcription of MnSOD by accelerating I?B? degradation in normal and cancer cells
    Article Snippet: .. RT-PCR Total cellular RNAs (1.0 μg) isolated with RNAiso Plus were used for cDNA preparation and then amplified by PCR kit (Takara Inc., Japan); First strand cDNA was synthesized using oligo-dT by reverse transcription reaction in 10 μl of reaction volume. .. The gene of interest was amplified by ExTaq polymerase in PCR kits using primer sequences described in the Additional file .

    Isolation:

    Article Title: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NF?B pathways which enhances transcription of MnSOD by accelerating I?B? degradation in normal and cancer cells
    Article Snippet: .. RT-PCR Total cellular RNAs (1.0 μg) isolated with RNAiso Plus were used for cDNA preparation and then amplified by PCR kit (Takara Inc., Japan); First strand cDNA was synthesized using oligo-dT by reverse transcription reaction in 10 μl of reaction volume. .. The gene of interest was amplified by ExTaq polymerase in PCR kits using primer sequences described in the Additional file .

    Produced:

    Article Title: The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori
    Article Snippet: .. RT-PCR was performed using the LA RNA PCR kit (Takara, www.takara-bio.co.jp ) following the manufacturer's instructions. cDNA was produced by random priming. .. RT-PCR primers were as follows: endogenous BmA3 , BmA3QPCR1F (5′-TACAATGAGCTGCGTGTCG-3′) and BmA3QPCR1R (5′-CGGGCGTGTTGAATGTTTC -3′); and Bmdsx mRNA transcribed from the transgene, TGM2F (5′-ATTGGCGGGACACGATC-3′) and TGM2R (5′-AGCGCTCCGTAGCACAA-3′).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NF?B pathways which enhances transcription of MnSOD by accelerating I?B? degradation in normal and cancer cells
    Article Snippet: .. RT-PCR Total cellular RNAs (1.0 μg) isolated with RNAiso Plus were used for cDNA preparation and then amplified by PCR kit (Takara Inc., Japan); First strand cDNA was synthesized using oligo-dT by reverse transcription reaction in 10 μl of reaction volume. .. The gene of interest was amplified by ExTaq polymerase in PCR kits using primer sequences described in the Additional file .

    Article Title: The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori
    Article Snippet: .. RT-PCR was performed using the LA RNA PCR kit (Takara, www.takara-bio.co.jp ) following the manufacturer's instructions. cDNA was produced by random priming. .. RT-PCR primers were as follows: endogenous BmA3 , BmA3QPCR1F (5′-TACAATGAGCTGCGTGTCG-3′) and BmA3QPCR1R (5′-CGGGCGTGTTGAATGTTTC -3′); and Bmdsx mRNA transcribed from the transgene, TGM2F (5′-ATTGGCGGGACACGATC-3′) and TGM2R (5′-AGCGCTCCGTAGCACAA-3′).

    Article Title: Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation
    Article Snippet: .. The sequence of the gene coding the gametocyte protein was amplified by reverse transcription polymerase chain reaction (RT-PCR) using the RNA LA PCR Kit (TaKaRa Bio. ..

    Article Title: Spermidine Synthase Genes Are Essential for Survival of Arabidopsis
    Article Snippet: .. RT-PCR was conducted by using the RNA LA PCR Kit (Takara, Kyoto) with 0.5 μ g of total RNA. ..

    Random Hexamer Labeling:

    Article Title: Murine lymph node-derived stromal cells effectively support survival but induce no activation/proliferation of peripheral resting T cells in vitro
    Article Snippet: .. Single-stranded cDNA was synthesized by reverse transcriptase (RT) with random hexamer oligonucleotides as primers, and the desired cDNA was amplified by polymerase chain reaction (PCR) using a TaKaRa RNA LA PCR kit (TaKaRa Biomedicals, Kyoto, Japan). .. Primers (sense and antisense) for the amplification of each cytokine cDNA were as follows: β-actin sense, TGG AAT CCT GTG GCA TCC ATG AAA C; β-actin antisense, TAA AAC GCA GCT CAG TAA CAG TCC G; IL-1α sense, CTC TAG AGC ACC ATG CTA CAG AC; IL-1α antisense, TGG AAT CCA GGG GAA ACA CTG; IL-2 sense, TGA TGG ACC TAC AGG AGC TCC TGA G; IL-2 antisense, GAG TCA AAT CCA GAA CAT GCC GCA G; IL-3 sense, GAA GTG GAT CCT GAG GAC AGA TAC G; IL-3 antisense, GAC CAT GGG CCA TGA GGA ACA TTC; IL-4 sense, CGA AGA ACA CCA CAG AGA GTG AGC T; IL-4 antisense, GAC TCA TTC ATG GTG CAG CTT ATC G; IL-5 sense, CTC TAG TAA GCC CAC TTC TA; IL-5 antisense, TGA TAC ATG AAT AAC ATC CC; IL-6 sense, TGG AGT CAC AGA AGG AGT GGC TAA G; IL-6 antisense, TCT GAC CAC AGA AGG AGT GGC TAA G; IL-7 sense, AAA TGC AGC TGA CTG CTG GC; IL-7 antisense, TCT CCA GTC TAA AAC AGG AC; IL-10 sense, TAC CTG GTA GAA GTG ATG CC; IL-10 antisense, CAT CAT GTA TGC TTC TAT GC; tumour necrosis factor-α (TNF-α) sense, GGC AGG TCT ACT TTG GAG TCA TTG C; TNF-α antisense, ACA TTC GAG GCT CCA GTG AAT TCG G; lymphotoxin (LT)-α sense, TGG CTG GGA ACA GGG GAA GGT TGA C; LT-α antisense, CGT GCT TTC TTC TAG AAC CCC TTG G. The conditions for PCR were 1 min at 94°, 2 min at 55° and 3 min at 72° for 30 cycles.

    Polymerase Chain Reaction:

    Article Title: Murine lymph node-derived stromal cells effectively support survival but induce no activation/proliferation of peripheral resting T cells in vitro
    Article Snippet: .. Single-stranded cDNA was synthesized by reverse transcriptase (RT) with random hexamer oligonucleotides as primers, and the desired cDNA was amplified by polymerase chain reaction (PCR) using a TaKaRa RNA LA PCR kit (TaKaRa Biomedicals, Kyoto, Japan). .. Primers (sense and antisense) for the amplification of each cytokine cDNA were as follows: β-actin sense, TGG AAT CCT GTG GCA TCC ATG AAA C; β-actin antisense, TAA AAC GCA GCT CAG TAA CAG TCC G; IL-1α sense, CTC TAG AGC ACC ATG CTA CAG AC; IL-1α antisense, TGG AAT CCA GGG GAA ACA CTG; IL-2 sense, TGA TGG ACC TAC AGG AGC TCC TGA G; IL-2 antisense, GAG TCA AAT CCA GAA CAT GCC GCA G; IL-3 sense, GAA GTG GAT CCT GAG GAC AGA TAC G; IL-3 antisense, GAC CAT GGG CCA TGA GGA ACA TTC; IL-4 sense, CGA AGA ACA CCA CAG AGA GTG AGC T; IL-4 antisense, GAC TCA TTC ATG GTG CAG CTT ATC G; IL-5 sense, CTC TAG TAA GCC CAC TTC TA; IL-5 antisense, TGA TAC ATG AAT AAC ATC CC; IL-6 sense, TGG AGT CAC AGA AGG AGT GGC TAA G; IL-6 antisense, TCT GAC CAC AGA AGG AGT GGC TAA G; IL-7 sense, AAA TGC AGC TGA CTG CTG GC; IL-7 antisense, TCT CCA GTC TAA AAC AGG AC; IL-10 sense, TAC CTG GTA GAA GTG ATG CC; IL-10 antisense, CAT CAT GTA TGC TTC TAT GC; tumour necrosis factor-α (TNF-α) sense, GGC AGG TCT ACT TTG GAG TCA TTG C; TNF-α antisense, ACA TTC GAG GCT CCA GTG AAT TCG G; lymphotoxin (LT)-α sense, TGG CTG GGA ACA GGG GAA GGT TGA C; LT-α antisense, CGT GCT TTC TTC TAG AAC CCC TTG G. The conditions for PCR were 1 min at 94°, 2 min at 55° and 3 min at 72° for 30 cycles.

    Article Title: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NF?B pathways which enhances transcription of MnSOD by accelerating I?B? degradation in normal and cancer cells
    Article Snippet: .. RT-PCR Total cellular RNAs (1.0 μg) isolated with RNAiso Plus were used for cDNA preparation and then amplified by PCR kit (Takara Inc., Japan); First strand cDNA was synthesized using oligo-dT by reverse transcription reaction in 10 μl of reaction volume. .. The gene of interest was amplified by ExTaq polymerase in PCR kits using primer sequences described in the Additional file .

    Article Title: The Riemerella anatipestifer M949_RS01035 gene is involved in bacterial lipopolysaccharide biosynthesis
    Article Snippet: .. Primer pairs specific for Tn4351 (primers TN-1 and IS4351-F) were used to amplify the sequences adjacent to the insertion site using the LA PCR kit (TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China). .. The nucleotide sequence was compared to sequence in the National Center for Biotechnology Information database using the BLASTX program [ ].

    Article Title: The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori
    Article Snippet: .. RT-PCR was performed using the LA RNA PCR kit (Takara, www.takara-bio.co.jp ) following the manufacturer's instructions. cDNA was produced by random priming. .. RT-PCR primers were as follows: endogenous BmA3 , BmA3QPCR1F (5′-TACAATGAGCTGCGTGTCG-3′) and BmA3QPCR1R (5′-CGGGCGTGTTGAATGTTTC -3′); and Bmdsx mRNA transcribed from the transgene, TGM2F (5′-ATTGGCGGGACACGATC-3′) and TGM2R (5′-AGCGCTCCGTAGCACAA-3′).

    Article Title: Tomato yellow leaf curl virus intergenic siRNAs target a host long noncoding RNA to modulate disease symptoms
    Article Snippet: .. The 5’ flanking region of the sense transcripts of SlLNR1 were obtained by RNA ligase-mediated rapid amplification of 5' cDNA ends First Choice ® RLM-RACE Kit (Invitrogen, USA), according to the instructions of the manufacturer, and the 3’ end was verified by 3’ RACE PCR kit (TAKARA). .. Full sense transcript of the SlLNR1 was amplified with the primers designed according to the joint sequence by RT-PCR.

    Article Title: Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation
    Article Snippet: .. The sequence of the gene coding the gametocyte protein was amplified by reverse transcription polymerase chain reaction (RT-PCR) using the RNA LA PCR Kit (TaKaRa Bio. ..

    Article Title: Spermidine Synthase Genes Are Essential for Survival of Arabidopsis
    Article Snippet: .. RT-PCR was conducted by using the RNA LA PCR Kit (Takara, Kyoto) with 0.5 μ g of total RNA. ..

    Article Title: Characterization of a novel germline PALB2 duplication in a hereditary breast and ovarian cancer family
    Article Snippet: .. LR-PCR was performed using a forward primer in intron 12 and a reverse primer in 3′-untranslated region (UTR) of PALB2 and the TaKaRa LA PCR kit (TaKaRa, Clontech) following the manufacturer’s instructions. ..

    Sequencing:

    Article Title: Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation
    Article Snippet: .. The sequence of the gene coding the gametocyte protein was amplified by reverse transcription polymerase chain reaction (RT-PCR) using the RNA LA PCR Kit (TaKaRa Bio. ..

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  • 85
    TaKaRa single tube sybr green kit
    Induction of ROS and activation of the ERS pathway and ASK1/JNK by T. gondii in vitro . (A) Primary trophoblast cells were harvested after coculture for 0, 1, 2, 6, 12, and 24 h. RNA isolation and cDNA synthesis were performed per the conventional protocol. Quantitative real-time <t>RT-PCR</t> was conducted using the <t>SYBR</t> green method, and the mRNA fold induction values were calculated by the 2 −ΔΔ CT method. Data are means ± SDs of three independent experiments. (B) Primary cultured trophoblasts (10 6 /well) were directly treated with 100 μl of lysis buffer. GSH was detected according to the manufacturer's instructions. Three independent experiments were conducted. (C) The trophoblast lysates experimentally infected by T. gondii were collected for immunoblotting.
    Single Tube Sybr Green Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single tube sybr green kit/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    single tube sybr green kit - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    99
    TaKaRa single tube pcr kit
    Detection of <t>HTLV-1</t> proviruses integrated in FPM1 cells by Southern blot hybridization (a) and <t>long-PCR</t> (b) methods. In panel a, 20 μg of DNA extracted from MT-2 (lane 1) and FPM1 cells (lane 2) was digested with Eco RI and hybridized with radiolabeled HTLV-1-specific probe. In panel b, 500 ng of DNA from MOLT-4 (lane 1), TL-OmI (lane 2), FPM1 (lane 3), and MT-2 (lane 4) were subjected to long PCR with primers for 5′ and 3′ HTLV-1 LTR. The expected size of the PCR products from a full-length HTLV-1 provirus is 7.7 kbp.
    Single Tube Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single tube pcr kit/product/TaKaRa
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    single tube pcr kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    TaKaRa sybr premix ex taq kit
    Detection of <t>HTLV-1</t> proviruses integrated in FPM1 cells by Southern blot hybridization (a) and <t>long-PCR</t> (b) methods. In panel a, 20 μg of DNA extracted from MT-2 (lane 1) and FPM1 cells (lane 2) was digested with Eco RI and hybridized with radiolabeled HTLV-1-specific probe. In panel b, 500 ng of DNA from MOLT-4 (lane 1), TL-OmI (lane 2), FPM1 (lane 3), and MT-2 (lane 4) were subjected to long PCR with primers for 5′ and 3′ HTLV-1 LTR. The expected size of the PCR products from a full-length HTLV-1 provirus is 7.7 kbp.
    Sybr Premix Ex Taq Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr premix ex taq kit/product/TaKaRa
    Average 99 stars, based on 1538 article reviews
    Price from $9.99 to $1999.99
    sybr premix ex taq kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Induction of ROS and activation of the ERS pathway and ASK1/JNK by T. gondii in vitro . (A) Primary trophoblast cells were harvested after coculture for 0, 1, 2, 6, 12, and 24 h. RNA isolation and cDNA synthesis were performed per the conventional protocol. Quantitative real-time RT-PCR was conducted using the SYBR green method, and the mRNA fold induction values were calculated by the 2 −ΔΔ CT method. Data are means ± SDs of three independent experiments. (B) Primary cultured trophoblasts (10 6 /well) were directly treated with 100 μl of lysis buffer. GSH was detected according to the manufacturer's instructions. Three independent experiments were conducted. (C) The trophoblast lysates experimentally infected by T. gondii were collected for immunoblotting.

    Journal: Infection and Immunity

    Article Title: Reactive Oxygen Species-Triggered Trophoblast Apoptosis Is Initiated by Endoplasmic Reticulum Stress via Activation of Caspase-12, CHOP, and the JNK Pathway in Toxoplasma gondii Infection in Mice

    doi: 10.1128/IAI.06295-11

    Figure Lengend Snippet: Induction of ROS and activation of the ERS pathway and ASK1/JNK by T. gondii in vitro . (A) Primary trophoblast cells were harvested after coculture for 0, 1, 2, 6, 12, and 24 h. RNA isolation and cDNA synthesis were performed per the conventional protocol. Quantitative real-time RT-PCR was conducted using the SYBR green method, and the mRNA fold induction values were calculated by the 2 −ΔΔ CT method. Data are means ± SDs of three independent experiments. (B) Primary cultured trophoblasts (10 6 /well) were directly treated with 100 μl of lysis buffer. GSH was detected according to the manufacturer's instructions. Three independent experiments were conducted. (C) The trophoblast lysates experimentally infected by T. gondii were collected for immunoblotting.

    Article Snippet: To investigate apoptosis-associated or oxidative stress-associated molecules, quantitative real-time RT-PCR was performed using specific primer sets (Shenggong, Shanghai, China) and a single-tube SYBR green kit (TaKaRa, Tokyo, Japan) with an ABI 7500 real-time PCR system (Applied Biosystems, SA).

    Techniques: Activation Assay, In Vitro, Isolation, Quantitative RT-PCR, SYBR Green Assay, Cell Culture, Lysis, Infection

    Detection of HTLV-1 proviruses integrated in FPM1 cells by Southern blot hybridization (a) and long-PCR (b) methods. In panel a, 20 μg of DNA extracted from MT-2 (lane 1) and FPM1 cells (lane 2) was digested with Eco RI and hybridized with radiolabeled HTLV-1-specific probe. In panel b, 500 ng of DNA from MOLT-4 (lane 1), TL-OmI (lane 2), FPM1 (lane 3), and MT-2 (lane 4) were subjected to long PCR with primers for 5′ and 3′ HTLV-1 LTR. The expected size of the PCR products from a full-length HTLV-1 provirus is 7.7 kbp.

    Journal: Journal of Virology

    Article Title: Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax

    doi:

    Figure Lengend Snippet: Detection of HTLV-1 proviruses integrated in FPM1 cells by Southern blot hybridization (a) and long-PCR (b) methods. In panel a, 20 μg of DNA extracted from MT-2 (lane 1) and FPM1 cells (lane 2) was digested with Eco RI and hybridized with radiolabeled HTLV-1-specific probe. In panel b, 500 ng of DNA from MOLT-4 (lane 1), TL-OmI (lane 2), FPM1 (lane 3), and MT-2 (lane 4) were subjected to long PCR with primers for 5′ and 3′ HTLV-1 LTR. The expected size of the PCR products from a full-length HTLV-1 provirus is 7.7 kbp.

    Article Snippet: HTLV-1 provirus in 3 μl of the whole peripheral blood was detected by a nested PCR method by using the Single-Tube PCR Kit (Takara, Kyoto, Japan) with HTLV-1 pX-specific primers.

    Techniques: Southern Blot, Hybridization, Polymerase Chain Reaction

    RT-PCR analysis of HTLV-1 mRNA. A total of 300 ng of DNase-treated total RNA from MT-2 (a) or FPM1 (b) was subjected to 30 cycles of one-step RT-PCR with primers for G3PDH (lane 1), gag (lane 2), env (lane 3), and pX (lane 4). The expected size of the amplified products was 435 bp for G3PDH, 242 bp for HTLV-1 gag , 316 bp for env , and 145 bp for pX.

    Journal: Journal of Virology

    Article Title: Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax

    doi:

    Figure Lengend Snippet: RT-PCR analysis of HTLV-1 mRNA. A total of 300 ng of DNase-treated total RNA from MT-2 (a) or FPM1 (b) was subjected to 30 cycles of one-step RT-PCR with primers for G3PDH (lane 1), gag (lane 2), env (lane 3), and pX (lane 4). The expected size of the amplified products was 435 bp for G3PDH, 242 bp for HTLV-1 gag , 316 bp for env , and 145 bp for pX.

    Article Snippet: HTLV-1 provirus in 3 μl of the whole peripheral blood was detected by a nested PCR method by using the Single-Tube PCR Kit (Takara, Kyoto, Japan) with HTLV-1 pX-specific primers.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

    Identification of HTLV-1 flanking region of FPM1. A 1-μg portion of DNA was extracted from the peripheral blood cells of a F344/N rat inoculated with MT-2 cells (lane 1) and two rats inoculated with FPM1 cells (lanes 2 and 3) 37 weeks after inoculation. The DNA was subjected to PCR with primers specific for G3PDH (top), HTLV-1 pX (middle), and HTLV-1 flanking region of FPM1 (bottom).

    Journal: Journal of Virology

    Article Title: Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax

    doi:

    Figure Lengend Snippet: Identification of HTLV-1 flanking region of FPM1. A 1-μg portion of DNA was extracted from the peripheral blood cells of a F344/N rat inoculated with MT-2 cells (lane 1) and two rats inoculated with FPM1 cells (lanes 2 and 3) 37 weeks after inoculation. The DNA was subjected to PCR with primers specific for G3PDH (top), HTLV-1 pX (middle), and HTLV-1 flanking region of FPM1 (bottom).

    Article Snippet: HTLV-1 provirus in 3 μl of the whole peripheral blood was detected by a nested PCR method by using the Single-Tube PCR Kit (Takara, Kyoto, Japan) with HTLV-1 pX-specific primers.

    Techniques: Polymerase Chain Reaction