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Types of geometry and hydrodynamics of the microfluidic system. (a) Schematic designs of microfluidic channels in full view. (b) Schematic designs of microfluidic channels with and without micro constrictions containing a representative cell body (grey shapes: the shape is only a guide for the eye). The longitudinal section is a cut by a plane parallel to the plane of the chip, encompassing the microchannel axis. The transverse section is a cut by a plane perpendicular to the axis of the microfluidic channel. The color codes for each types of channels will be used in data presentations for all subsequent figures. (c) <t>COMSOL</t> <t>simulated</t> <t>flow</t> <t>rates</t> of various microfluidic channels at a constant applied pressure of 10 kPa. (d) Cell diameter distribution of suspended control SK-BR-3 and MDA-MB-231 cells. Error bars represent standard deviation. Kolmogorov-Smirnov test. P value: 0.013. (e) Brightfield images of SK-BR-3 and MDA-MB-231 cells before and while residing in the three types of micro constrictions at a constant applied pressure of 10 kPa.
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Types of geometry and hydrodynamics of the microfluidic system. (a) Schematic designs of microfluidic channels in full view. (b) Schematic designs of microfluidic channels with and without micro constrictions containing a representative cell body (grey shapes: the shape is only a guide for the eye). The longitudinal section is a cut by a plane parallel to the plane of the chip, encompassing the microchannel axis. The transverse section is a cut by a plane perpendicular to the axis of the microfluidic channel. The color codes for each types of channels will be used in data presentations for all subsequent figures. (c) <t>COMSOL</t> <t>simulated</t> <t>flow</t> <t>rates</t> of various microfluidic channels at a constant applied pressure of 10 kPa. (d) Cell diameter distribution of suspended control SK-BR-3 and MDA-MB-231 cells. Error bars represent standard deviation. Kolmogorov-Smirnov test. P value: 0.013. (e) Brightfield images of SK-BR-3 and MDA-MB-231 cells before and while residing in the three types of micro constrictions at a constant applied pressure of 10 kPa.
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Types of geometry and hydrodynamics of the microfluidic system. (a) Schematic designs of microfluidic channels in full view. (b) Schematic designs of microfluidic channels with and without micro constrictions containing a representative cell body (grey shapes: the shape is only a guide for the eye). The longitudinal section is a cut by a plane parallel to the plane of the chip, encompassing the microchannel axis. The transverse section is a cut by a plane perpendicular to the axis of the microfluidic channel. The color codes for each types of channels will be used in data presentations for all subsequent figures. (c) <t>COMSOL</t> <t>simulated</t> <t>flow</t> <t>rates</t> of various microfluidic channels at a constant applied pressure of 10 kPa. (d) Cell diameter distribution of suspended control SK-BR-3 and MDA-MB-231 cells. Error bars represent standard deviation. Kolmogorov-Smirnov test. P value: 0.013. (e) Brightfield images of SK-BR-3 and MDA-MB-231 cells before and while residing in the three types of micro constrictions at a constant applied pressure of 10 kPa.
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Types of geometry and hydrodynamics of the microfluidic system. (a) Schematic designs of microfluidic channels in full view. (b) Schematic designs of microfluidic channels with and without micro constrictions containing a representative cell body (grey shapes: the shape is only a guide for the eye). The longitudinal section is a cut by a plane parallel to the plane of the chip, encompassing the microchannel axis. The transverse section is a cut by a plane perpendicular to the axis of the microfluidic channel. The color codes for each types of channels will be used in data presentations for all subsequent figures. (c) COMSOL simulated flow rates of various microfluidic channels at a constant applied pressure of 10 kPa. (d) Cell diameter distribution of suspended control SK-BR-3 and MDA-MB-231 cells. Error bars represent standard deviation. Kolmogorov-Smirnov test. P value: 0.013. (e) Brightfield images of SK-BR-3 and MDA-MB-231 cells before and while residing in the three types of micro constrictions at a constant applied pressure of 10 kPa.

Journal: Scientific Reports

Article Title: Fluid shear stress coupled with narrow constrictions induce cell type-dependent morphological and molecular changes in SK-BR-3 and MDA-MB-231 cells

doi: 10.1038/s41598-020-63316-w

Figure Lengend Snippet: Types of geometry and hydrodynamics of the microfluidic system. (a) Schematic designs of microfluidic channels in full view. (b) Schematic designs of microfluidic channels with and without micro constrictions containing a representative cell body (grey shapes: the shape is only a guide for the eye). The longitudinal section is a cut by a plane parallel to the plane of the chip, encompassing the microchannel axis. The transverse section is a cut by a plane perpendicular to the axis of the microfluidic channel. The color codes for each types of channels will be used in data presentations for all subsequent figures. (c) COMSOL simulated flow rates of various microfluidic channels at a constant applied pressure of 10 kPa. (d) Cell diameter distribution of suspended control SK-BR-3 and MDA-MB-231 cells. Error bars represent standard deviation. Kolmogorov-Smirnov test. P value: 0.013. (e) Brightfield images of SK-BR-3 and MDA-MB-231 cells before and while residing in the three types of micro constrictions at a constant applied pressure of 10 kPa.

Article Snippet: The color codes for each types of channels will be used in data presentations for all subsequent figures. (c) COMSOL simulated flow rates of various microfluidic channels at a constant applied pressure of 10 kPa. (d) Cell diameter distribution of suspended control SK-BR-3 and MDA-MB-231 cells.

Techniques: Control, Standard Deviation