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Cell Signaling Technology Inc simplechip plus enzymatic chromatin ip kit
Simplechip Plus Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/simplechip plus enzymatic chromatin ip kit/product/Cell Signaling Technology Inc
Average 99 stars, based on 83 article reviews
Price from $9.99 to $1999.99
simplechip plus enzymatic chromatin ip kit - by Bioz Stars, 2020-02
99/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration
Article Snippet: Chromatin immunoprecipitation (ChIP) ChIP assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP kit (9004 S, Cell Signaling). .. ChIP DNA was purified and subjected to qPCR using iTaq Universal SYBR Green Supermix (1725124, Bio-Rad) on StepOnePlus real-time PCR system (Applied Biosystems).

Transfection:

Article Title: ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells
Article Snippet: Chromatin immunoprecipitation (ChIP) The ChIP assay was performed by SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer's instructions. .. In brief, SGC7901 cells were seeded in a 10 cm dish overnight and subsequently transiently transfected with the MAX/pCMV6-XL5 plasmid for an additional 48 h. The transfected cells were fixed with 1% formaldehyde, and the reaction was terminated by the glycine solution.

Cell Culture:

Article Title: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration
Article Snippet: Chromatin immunoprecipitation (ChIP) ChIP assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP kit (9004 S, Cell Signaling). .. Briefly, chromatin DNA was obtained from colon tissue or cultured organoids after fixation with formaldehyde and fragmented using micrococcal nuclease and sonication to generate lengths of ~150–900 bp.

Purification:

Article Title: MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
Article Snippet: Chromatin Immunoprecipitation (ChIP) assay ChIP was performed using SimpleChIP Plus Enzymatic chromatin IP Kit (Agarose Beads) (Cell Signaling, CAT9004) according to the manufacturer's recommendations. .. After reversal of protein-DNA cross-links, the DNA was purified using DNA purification spin columns.

Article Title: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration
Article Snippet: Chromatin immunoprecipitation (ChIP) ChIP assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP kit (9004 S, Cell Signaling). .. ChIP DNA was purified and subjected to qPCR using iTaq Universal SYBR Green Supermix (1725124, Bio-Rad) on StepOnePlus real-time PCR system (Applied Biosystems).

SYBR Green Assay:

Article Title: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration
Article Snippet: Chromatin immunoprecipitation (ChIP) ChIP assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP kit (9004 S, Cell Signaling). .. ChIP DNA was purified and subjected to qPCR using iTaq Universal SYBR Green Supermix (1725124, Bio-Rad) on StepOnePlus real-time PCR system (Applied Biosystems).

Incubation:

Article Title: miR‐181c Activates Mitochondrial Calcium Uptake by Regulating MICU1 in the Heart
Article Snippet: Chromatin Immunoprecipitation Assay Either mouse heart tissue or H9c2 cells were incubated in 1% formaldehyde for 10 minutes and then swirled and incubated in 10X glycine solution for 5 minutes. .. Cells or tissue were washed twice in PBS and resuspended in 1X Buffer A, 1M DTT, and 200x PIC from SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Danvers, MA).

Article Title: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration
Article Snippet: Chromatin immunoprecipitation (ChIP) ChIP assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP kit (9004 S, Cell Signaling). .. Digested chromatin was then incubated with control IgG (2729, Cell Signaling) or anti-acetyl-histone H3 Ab (06-599, EMD Millipore, 1:150 dilution) and precipitated using protein G agarose beads (9007, Cell Signaling).

DNA Purification:

Article Title: MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
Article Snippet: Chromatin Immunoprecipitation (ChIP) assay ChIP was performed using SimpleChIP Plus Enzymatic chromatin IP Kit (Agarose Beads) (Cell Signaling, CAT9004) according to the manufacturer's recommendations. .. After reversal of protein-DNA cross-links, the DNA was purified using DNA purification spin columns.

Sonication:

Article Title: miR‐181c Activates Mitochondrial Calcium Uptake by Regulating MICU1 in the Heart
Article Snippet: Cells or tissue were washed twice in PBS and resuspended in 1X Buffer A, 1M DTT, and 200x PIC from SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). .. Chromatin was sonicated in 3 sets of 10‐second pulses using a probe sonicator.

Article Title: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration
Article Snippet: Chromatin immunoprecipitation (ChIP) ChIP assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP kit (9004 S, Cell Signaling). .. Briefly, chromatin DNA was obtained from colon tissue or cultured organoids after fixation with formaldehyde and fragmented using micrococcal nuclease and sonication to generate lengths of ~150–900 bp.

Chromatin Immunoprecipitation:

Article Title: miR‐181c Activates Mitochondrial Calcium Uptake by Regulating MICU1 in the Heart
Article Snippet: .. Cells or tissue were washed twice in PBS and resuspended in 1X Buffer A, 1M DTT, and 200x PIC from SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). .. Sample homogenates were then incubated in micrococcal nuclease for 20 minutes in 37°C with frequent mixing.

Article Title: MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
Article Snippet: .. Chromatin Immunoprecipitation (ChIP) assay ChIP was performed using SimpleChIP Plus Enzymatic chromatin IP Kit (Agarose Beads) (Cell Signaling, CAT9004) according to the manufacturer's recommendations. ..

Article Title: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration
Article Snippet: .. Chromatin immunoprecipitation (ChIP) ChIP assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP kit (9004 S, Cell Signaling). .. Briefly, chromatin DNA was obtained from colon tissue or cultured organoids after fixation with formaldehyde and fragmented using micrococcal nuclease and sonication to generate lengths of ~150–900 bp.

Article Title: ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells
Article Snippet: .. Chromatin immunoprecipitation (ChIP) The ChIP assay was performed by SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer's instructions. .. In brief, SGC7901 cells were seeded in a 10 cm dish overnight and subsequently transiently transfected with the MAX/pCMV6-XL5 plasmid for an additional 48 h. The transfected cells were fixed with 1% formaldehyde, and the reaction was terminated by the glycine solution.

Plasmid Preparation:

Article Title: ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells
Article Snippet: Chromatin immunoprecipitation (ChIP) The ChIP assay was performed by SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer's instructions. .. In brief, SGC7901 cells were seeded in a 10 cm dish overnight and subsequently transiently transfected with the MAX/pCMV6-XL5 plasmid for an additional 48 h. The transfected cells were fixed with 1% formaldehyde, and the reaction was terminated by the glycine solution.

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    Cell Signaling Technology Inc chip plus enzymatic chromatin ip kit
    Chip Plus Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip plus enzymatic chromatin ip kit/product/Cell Signaling Technology Inc
    Average 90 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    chip plus enzymatic chromatin ip kit - by Bioz Stars, 2020-02
    90/100 stars
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