Journal: Microbial Cell Factories
Article Title: A high-throughput expression screening platform to optimize the production of antimicrobial peptides
Figure Lengend Snippet: Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) pLysS, Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, C43 OverExpress C43 (DE3) pLysS
Article Snippet: Transformation of E. coli expression strains Six different E. coli strains were used for expression screening: Rosetta-gami 2 (DE3) pLysS (Merck Millipore), Origami 2 (Merck Millipore), BL21 (DE3) (NEB), SHuffle T7 Express lysY (NEB), OverExpress C41 (DE3) pLysS (Sigma-Aldrich), OverExpress C43 (DE3) pLysS (Sigma-Aldrich).