shuffle strain c3030 (New England Biolabs)


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Shuffle Strain C3030, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/shuffle strain c3030/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Comparison of periplasmic and intracellular expression of Arabidopsis thionin proproteins in E. coli"
Article Title: Comparison of periplasmic and intracellular expression of Arabidopsis thionin proproteins in E. coli
Journal: Biotechnology Letters
doi: 10.1007/s10529-013-1180-z

Figure Legend Snippet: Cytoplamic expression of proTHI-TRX fusion proteins in strain C3030. For all fusion proteins, 1 ml of cell culture was pelleted and dissolved in 100 μl sample buffer. 10 μl from this extract and an equivalent amount for total soluble cytoplasmic fractions were separated on Tricine/SDS gels. (M) Protein marker, ( 1 ) un-induced crude extract, ( 2 ) induced crude extract, ( 3 ) total soluble fraction taken after cell lysis by sonication, ( 4 ) insoluble fraction after sonication. Red stars indicate the 25 kDa fusion protein
Techniques Used: Expressing, Cell Culture, Marker, Lysis, Sonication

Figure Legend Snippet: Comparison of Ni–NTA purified proTHI-TRX fusion proteins from strain C3030. a Coomassie Brilliant Blue staining. b Western blot with anti-His tag antibody. Each well contained 3 μg protein. (M) Prestained protein marker, ( 1 ) proTHI2.1-TRX, ( 2 ) proTHI2.2-TRX, ( 3 ) proTHI2.3-TRX, ( 4 ) proTHI2.4-TRX
Techniques Used: Purification, Staining, Western Blot, Marker
2) Product Images from "Comparison of periplasmic and intracellular expression of Arabidopsis thionin proproteins in E. coli"
Article Title: Comparison of periplasmic and intracellular expression of Arabidopsis thionin proproteins in E. coli
Journal: Biotechnology Letters
doi: 10.1007/s10529-013-1180-z

Figure Legend Snippet: Cytoplamic expression of proTHI-TRX fusion proteins in strain C3030. For all fusion proteins, 1 ml of cell culture was pelleted and dissolved in 100 μl sample buffer. 10 μl from this extract and an equivalent amount for total soluble cytoplasmic fractions were separated on Tricine/SDS gels. (M) Protein marker, ( 1 ) un-induced crude extract, ( 2 ) induced crude extract, ( 3 ) total soluble fraction taken after cell lysis by sonication, ( 4 ) insoluble fraction after sonication. Red stars indicate the 25 kDa fusion protein
Techniques Used: Expressing, Cell Culture, Marker, Lysis, Sonication

Figure Legend Snippet: Comparison of Ni–NTA purified proTHI-TRX fusion proteins from strain C3030. a Coomassie Brilliant Blue staining. b Western blot with anti-His tag antibody. Each well contained 3 μg protein. (M) Prestained protein marker, ( 1 ) proTHI2.1-TRX, ( 2 ) proTHI2.2-TRX, ( 3 ) proTHI2.3-TRX, ( 4 ) proTHI2.4-TRX
Techniques Used: Purification, Staining, Western Blot, Marker