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shctrl constructs  (OriGene)


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    Structured Review

    OriGene shctrl constructs
    Shctrl Constructs, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+construct+shctrl/Scrambled+shRNA+control+in+pGFP-C-shLenti+shRNA+Vector/pm38873940-270-3-8
    Average 96 stars, based on 207 article reviews
    shctrl constructs - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Negative Control:

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFP-C-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [ ]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons.
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFPC-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [14]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    shRNA:

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFP-C-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [ ]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation
    Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (catalog #TL706020). ..

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons.
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFPC-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [14]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation
    Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (Cat. no. TL706020). ..

    Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage.
    Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice.

    Construct:

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFP-C-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [ ]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation
    Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (catalog #TL706020). ..

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons.
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFPC-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [14]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation
    Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (Cat. no. TL706020). ..

    Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage.
    Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice.

    Knockdown:

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFP-C-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [ ]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation
    Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (catalog #TL706020). ..

    Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons.
    Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled negative control shRNA (shCTRL, Origene TR30021) and a pool of four mouse gene-specific Nup358 shRNA constructs (shNup358, Origene TL501860) in pGFPC-shLenti Vector (Origene, Rockville, MD, USA) plasmids were used for the knockdown experiments as previously described [14]. .. Briefly, mouse cortical neurons were transfected on DIV 5 with shRNA constructs using Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions and processed on DIV 14.

    Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation
    Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (Cat. no. TL706020). ..

    Mutagenesis:

    Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation
    Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (catalog #TL706020). ..

    Synthesized:

    Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation
    Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (catalog #TL706020). ..

    Control:

    Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation
    Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (catalog #TL706020). ..

    Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation
    Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and KIF13A knockdown constructs (shCtrl, shKIF13A1 and A2, KIF13B) were purchased from Origene (Cat. no. TL706020). ..

    Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage.
    Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice.

    other:

    Article Title: The Role of Apelin and Apelin Receptor Expression in Migration and Invasiveness of Colon Cancer Cells.
    Article Snippet: To silence the APLN gene, cells were transfected with 29-mer small hairpin RNA (shRNA) constructs directed against human apelin or 29-mer non-targeting shRNA (shCTRL), which were purchased from OriGene (Rockville, MD, USA).

    Plasmid Preparation:

    Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage.
    Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice.

    In Vivo:

    Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage.
    Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice.

    Transfection:

    Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage.
    Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice.



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    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based <t>shRNA</t> silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of <t>normalized</t> <t>shCTRL</t> samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.
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    Image Search Results


    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.

    Journal: Cancers

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells

    doi: 10.3390/cancers13123055

    Figure Lengend Snippet: Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.

    Article Snippet: Non-targeting shRNA construct (shCTRL; source clone ID: RHS4348) and shITGA6 plasmid mapping against ITGA6 exon 15 (target sequence: AGGATATTGCTTTAGAAAT; source clone ID: V3LHS_326014) were purchased from Thermo-Scientific.

    Techniques: Western Blot, shRNA, MANN-WHITNEY, Marker