shctrl constructs (OriGene)
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Shctrl Constructs, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+construct+shctrl/Scrambled+shRNA+control+in+pGFP-C-shLenti+shRNA+Vector/pm38873940-270-3-8
Average 96 stars, based on 207 article reviews
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Negative Control:Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons. Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled shRNA:Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons. Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage. Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice. Construct:Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons. Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage. Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice. Knockdown:Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 °C and 5% CO 2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and Article Title: Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons. Article Snippet: The cells were grown in neuronal complete medium (Neurobasal 1×, B-27 supplement 1×, 0.5 mM L-glutamine, 10 μg/mL gentamicin; B-27 supplement and Neurobasal medium were purchased at Life Technologies, Carlsbad, CA, USA) at 37 ◦C and 5% CO2 until full maturation, i.e., 14 days in vitro (DIV). .. Scrambled Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and Mutagenesis:Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and Synthesized:Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and Control:Article Title: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation Article Snippet: Full-length human Hrs (NCBI accession # D84064.1 ) was synthesized at Genewiz and cloned into pFUGWm vector at BsrGI/XhoI sites to create pFU-EGFP-Hrs-W, and EGFP replaced with Halotag/mCherry at the AgeI/BsrGI sites to create pFU-Halo-Hrs-W/pFU-mCherry-Hrs. .. To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. shRNA scrambled control and Article Title: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation Article Snippet: To create pFU-EGFP-R183A Hrs-W, an 868 nucleotides fragment of Hrs containing the R183A mutation was synthesized at Genewiz and subcloned into the BsrGI/PshAI sites. .. Full-length [Chmp4b] shRNA scrambled control and Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage. Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice. other:Article Title: The Role of Apelin and Apelin Receptor Expression in Migration and Invasiveness of Colon Cancer Cells. Article Snippet: To silence the Plasmid Preparation:Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage. Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice. In Vivo:Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage. Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice. Transfection:Article Title: MBTPS2 exacerbates albuminuria in streptozotocin-induced type I diabetic nephropathy by promoting endoplasmic reticulum stress-mediated renal damage. Article Snippet: Background: The membrane-bound transcription factor protease site 2 (MBTPS2) is an intramembranous metalloprotease involved in the regulation of ER stress response, however, whether it is associated with DN is unknown.. Results: We report that MBTPS2 expression is upregulated in the renal cortex of diabetic mice induced by streptozotocin (STZ), a murine model of insulinopenic type 1 DN.. Functionally, in vivo, MBTPS2 overexpression exacerbates and its knockdown attenuates albuminuria, which indicate a detrimental role of MBTPS2 played in albuminuria development in DN mice. |
