Structured Review

Promega shrimp alkaline phosphatase
Shrimp Alkaline Phosphatase, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/shrimp alkaline phosphatase/product/Promega
Average 96 stars, based on 97 article reviews
Price from $9.99 to $1999.99
shrimp alkaline phosphatase - by Bioz Stars, 2020-09
96/100 stars

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Incubation:

Article Title: Human Immunodeficiency Virus Type 1 gp41 Antibodies That Mask Membrane Proximal Region Epitopes: Antibody Binding Kinetics, Induction, and Potential for Regulation in Acute Infection ▿
Article Snippet: .. Inhibition of biotin-MAb binding was detected with streptavidin-alkaline phosphatase at 1:1,000 (Promega V5591), followed by incubation with alkaline phosphatase substrate (2 mM MgCl2 , 1 mg/ml p -NPP, CBC, pH 9.6). ..

Article Title: Vascular-directed responses of microglia produced by methamphetamine exposure: indirect evidence that microglia are involved in vascular repair?
Article Snippet: .. Then sections were washed in TBS containing Triton-X for 3–5 min, and incubated in Streptavidin-Alkaline Phosphatase (1:200; Promega, USA) for 2 h are RT. ..

Article Title: Frontal affinity chromatography analysis of constructs of DC‐SIGN, DC‐SIGNR and LSECtin extend evidence for affinity to agalactosylated N‐glycans
Article Snippet: .. After washing with NaCl/Tris‐T, the membrane was incubated with streptavidin‐conjugated alkaline phosphatase (Promega, Tokyo, Japan) for 30 min at room temperature. .. After washing with NaCl/Tris‐T, the membrane was visualized with Western Blue‐stabilized substrate for alkaline phosphatase (Promega).

Article Title: Prenatal Lead Levels, Plasma Amyloid ? Levels, and Gene Expression in Young Adulthood
Article Snippet: .. Finally, samples were incubated with streptavidin alkaline phosphatase (Promega, Madison, WI) in PBS for 1 hr at room temperature and washed three times with Tris-buffered saline. .. After adding AttoPhos (Promega), the signal was amplified and measured with a Victor2 microtiter plate reader (PerkinElmer, Boston, MA).

Article Title: Ribosomal stalling landscapes revealed by high-throughput inverse toeprinting of mRNA libraries
Article Snippet: .. The milk solution was removed and the membrane was incubated with streptavidin-alkaline phosphatase antibody (Promega) in a 1:1,000 dilution in TBS-T for 1 h at room temperature. ..

Inhibition:

Article Title: Human Immunodeficiency Virus Type 1 gp41 Antibodies That Mask Membrane Proximal Region Epitopes: Antibody Binding Kinetics, Induction, and Potential for Regulation in Acute Infection ▿
Article Snippet: .. Inhibition of biotin-MAb binding was detected with streptavidin-alkaline phosphatase at 1:1,000 (Promega V5591), followed by incubation with alkaline phosphatase substrate (2 mM MgCl2 , 1 mg/ml p -NPP, CBC, pH 9.6). ..

Binding Assay:

Article Title: Human Immunodeficiency Virus Type 1 gp41 Antibodies That Mask Membrane Proximal Region Epitopes: Antibody Binding Kinetics, Induction, and Potential for Regulation in Acute Infection ▿
Article Snippet: .. Inhibition of biotin-MAb binding was detected with streptavidin-alkaline phosphatase at 1:1,000 (Promega V5591), followed by incubation with alkaline phosphatase substrate (2 mM MgCl2 , 1 mg/ml p -NPP, CBC, pH 9.6). ..

Article Title: Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG
Article Snippet: .. Biotin-mAb binding was detected with streptavidin alkaline phosphatase at 1:1,000 (Promega V5591) followed by substrate [carbonate bicarbonate buffer (CBC) buffer + 2 mM MgCl2 + 1 mg/mL 4-nitrophenyl phosphate di(2-amino-2-ethyl-1,3-propanediol) salt]. ..

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  • 85
    Promega shrimp alkaline phosphatase sirnas
    Immunostimulatory properties of <t>T7-siRNAs</t> and c-siRNAs in chicken cells. IFN-β mRNA levels were measured in DF-1 cells transfected with T7-siRNAs (white bars, 20 pmol) or c-siRNAs (black bars, 20 pmol) at (A) 8 h or (B) 24 h. a = p
    Shrimp Alkaline Phosphatase Sirnas, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase sirnas/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase sirnas - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    91
    Promega shrimp alkaline phosphatase sap
    ΔNp63α overexpression results in increased nuclear levels of transcriptionally active phosphorylated c-Rel in murine keratinocytes A) Elevated Δ Np63α expression specifically enhances nuclear levels of the NF-κ B subunit c-Rel. Western blots of nuclear extracts from primary murine keratinocytes harvested 21h post-adenoviral introduction of human ΔNp63α or β-gal. Cultures were maintained in medium containing 0.05mM Ca 2+ throughout or exposed to 0.12 mM Ca 2+ for the final 4h. C-Rel levels are increased in the nuclei of ΔNp63α - overexpressing keratinocytes. B) c-Rel is phosphorylated in response to elevated ΔNp63α. Western analysis of nuclear extracts from keratinocytes overexpressing ΔNp63α or β-gal cultured under control conditions (top) or in the presence of the thiol modifier N-ethylmaleimide (NEM) at concentrations noted for 21h following adenoviral introduction to block in vivo phosphorylation (bottom left). The upper species is eliminated in the presence of NEM. Phosphorylation was confirmed by western analysis of nuclear extracts derived from keratinocytes overexpressing ΔNp63α incubated in the presence or absence of Shrimp Alkaline Phosphatase <t>(SAP)</t> for <t>3h</t> at 37°C (bottom right). A non-incubated control nuclear extract (NE) is included for visual reference. SAP treatment results in the loss of the upper phosphorylated species. C) ΔNp63α overexpression results in NF-κ B-mediated transactivation; α-domain of ΔNp63α required. NF-κB responsive reporter gene activity in keratinocytes overexpressing ΔNp63α, ΔNp63 p40 (ΔNp63 lacking α-domain) or β-gal. Samples were harvested 24h post-transfection. Results depict mean +/− s.d. of triplicate samples of a representative experiment performed thrice. D) c-Rel is required for NF-κ B-mediated transactivation following ΔNp63α overexpression. Keratinocytes were co-transfected with NF-κB reporter construct and c-Rel-targeting siRNA or control siRNA, 24h prior to adenoviral infection with ΔNp63α or β-gal. Samples were harvested 24h post adenoviral introduction. Right side: Western blot reveals depletion of c-Rel in whole cell lysates at time of harvest (NT: non targeting siRNA). Left side: Fold increase in NF-κB reporter gene activity in ΔNp63α overexpressing cultures compared to β-gal control cultures in the presence or absence of c-Rel siRNA. Triplicate wells were averaged and are presented as fold increase relative to β-gal controls that are normalized to 1.0. Experiment was performed twice with consistent results; representative experiment shown.
    Shrimp Alkaline Phosphatase Sap, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase sap/product/Promega
    Average 91 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase sap - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Immunostimulatory properties of T7-siRNAs and c-siRNAs in chicken cells. IFN-β mRNA levels were measured in DF-1 cells transfected with T7-siRNAs (white bars, 20 pmol) or c-siRNAs (black bars, 20 pmol) at (A) 8 h or (B) 24 h. a = p

    Journal: PLoS ONE

    Article Title: Immunostimulatory Motifs Enhance Antiviral siRNAs Targeting Highly Pathogenic Avian Influenza H5N1

    doi: 10.1371/journal.pone.0021552

    Figure Lengend Snippet: Immunostimulatory properties of T7-siRNAs and c-siRNAs in chicken cells. IFN-β mRNA levels were measured in DF-1 cells transfected with T7-siRNAs (white bars, 20 pmol) or c-siRNAs (black bars, 20 pmol) at (A) 8 h or (B) 24 h. a = p

    Article Snippet: Treatment of siRNAs with shrimp alkaline phosphatase siRNAs synthesized using T7 RNA polymerase were treated with shrimp alkaline phosphatase (SAP) according to manufacturers guidelines (Promega).

    Techniques: Transfection

    ΔNp63α overexpression results in increased nuclear levels of transcriptionally active phosphorylated c-Rel in murine keratinocytes A) Elevated Δ Np63α expression specifically enhances nuclear levels of the NF-κ B subunit c-Rel. Western blots of nuclear extracts from primary murine keratinocytes harvested 21h post-adenoviral introduction of human ΔNp63α or β-gal. Cultures were maintained in medium containing 0.05mM Ca 2+ throughout or exposed to 0.12 mM Ca 2+ for the final 4h. C-Rel levels are increased in the nuclei of ΔNp63α - overexpressing keratinocytes. B) c-Rel is phosphorylated in response to elevated ΔNp63α. Western analysis of nuclear extracts from keratinocytes overexpressing ΔNp63α or β-gal cultured under control conditions (top) or in the presence of the thiol modifier N-ethylmaleimide (NEM) at concentrations noted for 21h following adenoviral introduction to block in vivo phosphorylation (bottom left). The upper species is eliminated in the presence of NEM. Phosphorylation was confirmed by western analysis of nuclear extracts derived from keratinocytes overexpressing ΔNp63α incubated in the presence or absence of Shrimp Alkaline Phosphatase (SAP) for 3h at 37°C (bottom right). A non-incubated control nuclear extract (NE) is included for visual reference. SAP treatment results in the loss of the upper phosphorylated species. C) ΔNp63α overexpression results in NF-κ B-mediated transactivation; α-domain of ΔNp63α required. NF-κB responsive reporter gene activity in keratinocytes overexpressing ΔNp63α, ΔNp63 p40 (ΔNp63 lacking α-domain) or β-gal. Samples were harvested 24h post-transfection. Results depict mean +/− s.d. of triplicate samples of a representative experiment performed thrice. D) c-Rel is required for NF-κ B-mediated transactivation following ΔNp63α overexpression. Keratinocytes were co-transfected with NF-κB reporter construct and c-Rel-targeting siRNA or control siRNA, 24h prior to adenoviral infection with ΔNp63α or β-gal. Samples were harvested 24h post adenoviral introduction. Right side: Western blot reveals depletion of c-Rel in whole cell lysates at time of harvest (NT: non targeting siRNA). Left side: Fold increase in NF-κB reporter gene activity in ΔNp63α overexpressing cultures compared to β-gal control cultures in the presence or absence of c-Rel siRNA. Triplicate wells were averaged and are presented as fold increase relative to β-gal controls that are normalized to 1.0. Experiment was performed twice with consistent results; representative experiment shown.

    Journal: Cancer research

    Article Title: The p53 homologue ?Np63? interacts with the NF-?B pathway to modulate epithelial cell growth

    doi: 10.1158/0008-5472.CAN-07-6123

    Figure Lengend Snippet: ΔNp63α overexpression results in increased nuclear levels of transcriptionally active phosphorylated c-Rel in murine keratinocytes A) Elevated Δ Np63α expression specifically enhances nuclear levels of the NF-κ B subunit c-Rel. Western blots of nuclear extracts from primary murine keratinocytes harvested 21h post-adenoviral introduction of human ΔNp63α or β-gal. Cultures were maintained in medium containing 0.05mM Ca 2+ throughout or exposed to 0.12 mM Ca 2+ for the final 4h. C-Rel levels are increased in the nuclei of ΔNp63α - overexpressing keratinocytes. B) c-Rel is phosphorylated in response to elevated ΔNp63α. Western analysis of nuclear extracts from keratinocytes overexpressing ΔNp63α or β-gal cultured under control conditions (top) or in the presence of the thiol modifier N-ethylmaleimide (NEM) at concentrations noted for 21h following adenoviral introduction to block in vivo phosphorylation (bottom left). The upper species is eliminated in the presence of NEM. Phosphorylation was confirmed by western analysis of nuclear extracts derived from keratinocytes overexpressing ΔNp63α incubated in the presence or absence of Shrimp Alkaline Phosphatase (SAP) for 3h at 37°C (bottom right). A non-incubated control nuclear extract (NE) is included for visual reference. SAP treatment results in the loss of the upper phosphorylated species. C) ΔNp63α overexpression results in NF-κ B-mediated transactivation; α-domain of ΔNp63α required. NF-κB responsive reporter gene activity in keratinocytes overexpressing ΔNp63α, ΔNp63 p40 (ΔNp63 lacking α-domain) or β-gal. Samples were harvested 24h post-transfection. Results depict mean +/− s.d. of triplicate samples of a representative experiment performed thrice. D) c-Rel is required for NF-κ B-mediated transactivation following ΔNp63α overexpression. Keratinocytes were co-transfected with NF-κB reporter construct and c-Rel-targeting siRNA or control siRNA, 24h prior to adenoviral infection with ΔNp63α or β-gal. Samples were harvested 24h post adenoviral introduction. Right side: Western blot reveals depletion of c-Rel in whole cell lysates at time of harvest (NT: non targeting siRNA). Left side: Fold increase in NF-κB reporter gene activity in ΔNp63α overexpressing cultures compared to β-gal control cultures in the presence or absence of c-Rel siRNA. Triplicate wells were averaged and are presented as fold increase relative to β-gal controls that are normalized to 1.0. Experiment was performed twice with consistent results; representative experiment shown.

    Article Snippet: Nuclear extracts were incubated in 1X SAP buffer +/− 10U Shrimp Alkaline Phosphatase (SAP) (Promega, Madison, WI) for 3h at 37°C followed by inactivation at 65°C.

    Techniques: Over Expression, Expressing, Western Blot, Cell Culture, Blocking Assay, In Vivo, Derivative Assay, Incubation, Activity Assay, Transfection, Construct, Infection