shrimp alkaline phosphatase  (New England Biolabs)


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    Name:
    Shrimp Alkaline Phosphatase rSAP
    Description:
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    Catalog Number:
    m0371l
    Price:
    244
    Size:
    2 500 units
    Category:
    Alkaline Phosphatases
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    Structured Review

    New England Biolabs shrimp alkaline phosphatase
    Shrimp Alkaline Phosphatase rSAP
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    https://www.bioz.com/result/shrimp alkaline phosphatase/product/New England Biolabs
    Average 90 stars, based on 138 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: All the plasmids assembled using yeast cloning were purified from yeast using Zymoprep Yeast Plasmid Miniprep II according to the manufacturer's protocol and transformed into electrocompetent E. coli strain S17 by electroporation. .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product.

    Amplification:

    Article Title: Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty
    Article Snippet: The immunoprecipitated DNA was amplified before library preparation and deep-sequencing. .. Shortly, 10 µl of immunoprecipitated DNA were dephosphorylated using Shrimp Alkaline Phosphatase (NEB, 1 U) for 10 min at 37 °C.

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: A sample (6 µL) of the PCR product was electrophoresed through a 1% TAE agarose gel to confirm the size of the amplicon. .. PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ).

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: The primers were 5′-YCAGCTATGACCATGATTCAGTGGTATCCTCC-3′ and 5′-Y TCGGTGCGGGCCTCTTCGCTATTAC-3′ (‘Y’ is an amino group), and the amplification conditions consisted of 10 s at 98 °C, 30 s at 62 °C, 15 s at 72 °C for 30 cycles, followed by a final extension at 72 °C for 5 min. .. For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase.

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: Paragraph title: DNA extraction and amplification ... We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India).

    Article Title: Contrasting life histories contribute to divergent patterns of genetic diversity and population connectivity in freshwater sculpin fishes
    Article Snippet: .. The amplified PCR products were purified enzymatically with Exonuclease I (New England BioLabs, Ipswich, MA, USA) and Shrimp Alkaline Phosphatase (New England BioLabs). .. The purified mtDNA fragments were subjected to direct sequencing using the internal forward LCCR and the same reverse H12Sr5 primers as in the PCR, and the BigDye Terminator 3.1 Cycle Sequencing Ready Reaction Kit in an ABI 3730xl automated DNA sequencer (Applied Biosystems).

    Article Title: A new Hermeuptychia (Lepidoptera, Nymphalidae, Satyrinae) is sympatric and synchronic with H. sosybius in southeast US coastal plains, while another new Hermeuptychia species - not hermes - inhabits south Texas and northeast Mexico
    Article Snippet: .. PCR reaction was cleaned up by enzymatic digestion for the whole barcode amplifications of DNA from freshly collected or alcohol preserved specimens and ID tag amplification of old specimens with 4 μl Shrimp Alkaline Phosphatase (20 U/μl) and 1 ul Exonuclease I (1 U/μl) from New England Biolabs. .. For older specimens that are barcoded in multiple segments, due to the frequent presence of primer dimers and other short non-specific PCR products, Agencourt Ampure XP beads or Invitrogen E-Gel® EX Agarose Gels (followed by Zymo gel DNA recovery kit) were used to select the DNA products of expected length.

    Article Title: Expanding and testing fluorescent amplified fragment length polymorphisms for identifying roots of boreal forest plant species
    Article Snippet: .. Amplified DNA was cleaned using ExoSAP (exonuclease 1 10 units·μL−1 [New England Biolabs M0293S; New England Biolabs, Ipswich, Massachusetts, USA] and shrimp alkaline phosphatase 1 unit·μL−1 [New England Biolabs M0371S]) following the manufacturer's protocol. .. Big Dye sequencing reactions and bidirectional sequencing were performed on an ABI 3730 DNA Analyzer (Applied Biosystems) carried out by the University of Alberta Molecular Biology Facility.

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product. .. All amplified DNA was treated with DpnI to remove any remaining template and then cleaned using a Qiagen PCR purification kit.

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: The primers were 5′-GCTAGCGGATGCATCGACTCCACAACAG-3′ and 5′-GGCTCCCTTTAGGGTTCCGATTTAGTG-3′, and the PCR amplification started at 95 °C for 2 min; then, 35 cycles at 95 °C for 30 s, 65 °C for 30 s, and 72 °C for 1.5 min, and a final 5-min extension at 72 °C. .. For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase.

    De-Phosphorylation Assay:

    Article Title: Accurate RNA consensus sequencing for high-fidelity detection of transcriptional mutagenesis-induced epimutations
    Article Snippet: .. End-repair of the resulting fragmented RNA was accomplished in two steps: ( i ) dephosphorylation of the 5′- and 3′-ends by shrimp alkaline phosphatase (NEB) and ( ii ) 5′-phosphorylation by OptiKinase, a polynucleotide kinase (PNK) mutant (Affymetrix), both per the manufacturers’ protocols. ..

    Synthesized:

    Article Title: Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty
    Article Snippet: Shortly, 10 µl of immunoprecipitated DNA were dephosphorylated using Shrimp Alkaline Phosphatase (NEB, 1 U) for 10 min at 37 °C. .. RNA was then synthesized by in vitro transcription using T7 RNA polymerase and the RNAMaxx high yield kit (Agilent) in an overnight reaction at 37 °C.

    Construct:

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: The spoT complementation construct was made by linearizing the pMQ30 vector using EcoRI. .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product.

    Incubation:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: .. PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ). .. Using the Cavendish PDS cDNA sequence (GenBank Accession ), primers were designed to amplify a short 5′ segment of the PDS gene from cv.

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase. .. To the above reaction mixture was added Tsp509I (10 U) and the solution was incubated at 65 °C for 1 hr, followed by quenching with 15 μL formamide gel loading buffer containing xylene cyanol FF and bromophenol blue dyes.

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product. .. The vector and spoT fragments were assembled using NEBuilder HiFi DNA assembly master mix and incubated at 60°C for 1 h. The DNA was then transformed into high-efficiency DH5α chemically competent cells.

    Article Title: Multiple Divergent Haplotypes Express Completely Distinct Sets of Class I MHC Genes in Zebrafish
    Article Snippet: .. Prior to sequencing, PCR reactions (5 μL aliquots) were first mixed with 3 units of Exonuclease (Affymetrix) and 0.3 units of Shrimp Alkaline Phosphatase (NEB) and incubated at 37°C for 30 minutes followed by inactivation at 80°C for 15 minutes. .. Sequences were analyzed using SeqMan (DNAstar) to identify specific SNPs and assign haplotypes ( ).

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase. .. To the reaction mixture 5 units of SfaNI was subsequently added, and the solution was incubated at 37 °C for 1 h, followed by quenching with 15 μl of formamide gel loading buffer.

    Genome Wide:

    Article Title: Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty
    Article Snippet: Paragraph title: Genome-wide ChIP assays ... Shortly, 10 µl of immunoprecipitated DNA were dephosphorylated using Shrimp Alkaline Phosphatase (NEB, 1 U) for 10 min at 37 °C.

    Modification:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: Paragraph title: Sequence analysis of CRISPR/Cas9 modified plants ... PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ).

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: The rhlI promoter region was amplified from PA14 genomic DNA (gDNA) using Phusion high-fidelity DNA polymerase with primer tails homologous to the modified pMQ30 ATT KI vector containing the lacZ-GFP reporters. .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product.

    Transformation Assay:

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: All the plasmids assembled using yeast cloning were purified from yeast using Zymoprep Yeast Plasmid Miniprep II according to the manufacturer's protocol and transformed into electrocompetent E. coli strain S17 by electroporation. .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product.

    Hybridization:

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs). .. The labeled PCR products were purified using the QIAquick nucleotide removal kit (QIAGEN, Valencia, CA) prior to hybridization.

    Electroporation:

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: All the plasmids assembled using yeast cloning were purified from yeast using Zymoprep Yeast Plasmid Miniprep II according to the manufacturer's protocol and transformed into electrocompetent E. coli strain S17 by electroporation. .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product.

    Southern Blot:

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: Paragraph title: Southern blot analysis: ... The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs).

    Transferring:

    Article Title: Accurate RNA consensus sequencing for high-fidelity detection of transcriptional mutagenesis-induced epimutations
    Article Snippet: End-repair of the resulting fragmented RNA was accomplished in two steps: ( i ) dephosphorylation of the 5′- and 3′-ends by shrimp alkaline phosphatase (NEB) and ( ii ) 5′-phosphorylation by OptiKinase, a polynucleotide kinase (PNK) mutant (Affymetrix), both per the manufacturers’ protocols. .. RNA fragments larger than the optimal range of 150–300 nt were removed by adding 1.5 vol of AMPure RNA CleanXP beads (Agencourt) to end-repaired RNA and transferring the supernatant to a separate tube.

    Generated:

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: All probes were generated by PCR: the 661-bp lac probe was generated using the oligos 5′-TGAATTACATTCCCAACCGCGT-3′ and 5′-ACCAACGCGCAGCCCGGAC-3′; the 1050-bp traT probe was generated using the oligos 5′-ATATCAATTTGTTGGTG-3′ and 5′-GTCGGACTGATTATTAATG-3′; and the 639-bp dnaQ probe was generated using the oligos 5′-GCTTAAGCGCGATATTCC-3′ and 5′-CTTGCGGCTCTCTGAAC-3′. .. The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs).

    Imaging:

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: The blot was then exposed to a storage phosphor screen (Kodak, Rochester, NY) and visualized using a Typhoon phosphor imaging system (Molecular Dynamics). .. The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs).

    Sequencing:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: .. PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ). .. Using the Cavendish PDS cDNA sequence (GenBank Accession ), primers were designed to amplify a short 5′ segment of the PDS gene from cv.

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India). .. All sequencing reactions were performed on ABI3100xl Automated system (Bangalore, India).

    Article Title: Contrasting life histories contribute to divergent patterns of genetic diversity and population connectivity in freshwater sculpin fishes
    Article Snippet: Paragraph title: MtDNA control region sequencing ... The amplified PCR products were purified enzymatically with Exonuclease I (New England BioLabs, Ipswich, MA, USA) and Shrimp Alkaline Phosphatase (New England BioLabs).

    Article Title: DMSO increases mutation-scanning detection sensitivity in clinical samples using high resolution melting
    Article Snippet: .. The PCR products were digested by Exonuclease I and Shrimp Alkaline Phosphatase (New England Biolabs) and processed for Sanger sequencing at Eton Bioscience Inc. (Boston, MA, USA). .. To enable sequencing of short PCR amplicons, a 30-T tail was added to the 5′-end of the forward primer ( ).

    Article Title: Expanding and testing fluorescent amplified fragment length polymorphisms for identifying roots of boreal forest plant species
    Article Snippet: Paragraph title: Sanger sequencing congeneric DNA ... Amplified DNA was cleaned using ExoSAP (exonuclease 1 10 units·μL−1 [New England Biolabs M0293S; New England Biolabs, Ipswich, Massachusetts, USA] and shrimp alkaline phosphatase 1 unit·μL−1 [New England Biolabs M0371S]) following the manufacturer's protocol.

    Article Title: Multiple Divergent Haplotypes Express Completely Distinct Sets of Class I MHC Genes in Zebrafish
    Article Snippet: .. Prior to sequencing, PCR reactions (5 μL aliquots) were first mixed with 3 units of Exonuclease (Affymetrix) and 0.3 units of Shrimp Alkaline Phosphatase (NEB) and incubated at 37°C for 30 minutes followed by inactivation at 80°C for 15 minutes. .. Sequences were analyzed using SeqMan (DNAstar) to identify specific SNPs and assign haplotypes ( ).

    Recombinant:

    Article Title: Improving the spectral analysis of fluorescence resonance energy transfer in live cells: Application to interferon receptors and Janus kinases
    Article Snippet: Restriction endonucleases, shrimp alkaline phosphatase and T4 DNA Ligase was purchased from New England BioLabs. .. Recombinant Taq DNA polymerase was purified as described previously [ ].

    DNA Extraction:

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: Paragraph title: DNA extraction and amplification ... We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India).

    Article Title: A new Hermeuptychia (Lepidoptera, Nymphalidae, Satyrinae) is sympatric and synchronic with H. sosybius in southeast US coastal plains, while another new Hermeuptychia species - not hermes - inhabits south Texas and northeast Mexico
    Article Snippet: For DNA extraction and PCR reactions, they were intermixed and ordered not by species, but as they were placed in USNM collection by curators who did not suspect the presence of more than one species (i.e. semi-randomly, according to DNA voucher numbers assigned to them). .. PCR reaction was cleaned up by enzymatic digestion for the whole barcode amplifications of DNA from freshly collected or alcohol preserved specimens and ID tag amplification of old specimens with 4 μl Shrimp Alkaline Phosphatase (20 U/μl) and 1 ul Exonuclease I (1 U/μl) from New England Biolabs.

    Molecular Cloning:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: .. PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ). .. Using the Cavendish PDS cDNA sequence (GenBank Accession ), primers were designed to amplify a short 5′ segment of the PDS gene from cv.

    In Vivo:

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: The progeny genomes arising from in vivo replication were amplified in a PCR (GoTaq green master mix, Promega) containing a pair of primers whose products cover the initial lesion site and span 8 DpnI recognition sites. .. For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase.

    Mutagenesis:

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: Paragraph title: Determination of the bypass efficiency and mutation frequency using competitive replication and adduct bypass (CRAB) and restriction endonuclease and post-labeling (REAP) assays ... For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase.

    Article Title: Accurate RNA consensus sequencing for high-fidelity detection of transcriptional mutagenesis-induced epimutations
    Article Snippet: .. End-repair of the resulting fragmented RNA was accomplished in two steps: ( i ) dephosphorylation of the 5′- and 3′-ends by shrimp alkaline phosphatase (NEB) and ( ii ) 5′-phosphorylation by OptiKinase, a polynucleotide kinase (PNK) mutant (Affymetrix), both per the manufacturers’ protocols. ..

    Isolation:

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: PCR amplification of the region of interest in the resulting isolated progeny genome was performed by using Phusion high-fidelity DNA polymerase. .. For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase.

    Article Title: Contrasting life histories contribute to divergent patterns of genetic diversity and population connectivity in freshwater sculpin fishes
    Article Snippet: Genomic DNA was isolated using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). .. The amplified PCR products were purified enzymatically with Exonuclease I (New England BioLabs, Ipswich, MA, USA) and Shrimp Alkaline Phosphatase (New England BioLabs).

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product. .. The plasmid was isolated using a Zyppy plasmid miniprep kit (Zymo Research, Irvine, CA) and then screened by digestion with NruI.

    Labeling:

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: .. The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs). .. The labeled PCR products were purified using the QIAquick nucleotide removal kit (QIAGEN, Valencia, CA) prior to hybridization.

    Purification:

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: The PCR products were purified by using QIAquick PCR purification kit (Qiagen, Valencia, CA). .. For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase.

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: .. We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India). .. All sequencing reactions were performed on ABI3100xl Automated system (Bangalore, India).

    Article Title: Contrasting life histories contribute to divergent patterns of genetic diversity and population connectivity in freshwater sculpin fishes
    Article Snippet: .. The amplified PCR products were purified enzymatically with Exonuclease I (New England BioLabs, Ipswich, MA, USA) and Shrimp Alkaline Phosphatase (New England BioLabs). .. The purified mtDNA fragments were subjected to direct sequencing using the internal forward LCCR and the same reverse H12Sr5 primers as in the PCR, and the BigDye Terminator 3.1 Cycle Sequencing Ready Reaction Kit in an ABI 3730xl automated DNA sequencer (Applied Biosystems).

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs). .. The labeled PCR products were purified using the QIAquick nucleotide removal kit (QIAGEN, Valencia, CA) prior to hybridization.

    Article Title: Improving the spectral analysis of fluorescence resonance energy transfer in live cells: Application to interferon receptors and Janus kinases
    Article Snippet: Restriction endonucleases, shrimp alkaline phosphatase and T4 DNA Ligase was purchased from New England BioLabs. .. Recombinant Taq DNA polymerase was purified as described previously [ ].

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product. .. All amplified DNA was treated with DpnI to remove any remaining template and then cleaned using a Qiagen PCR purification kit.

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: The PCR products were purified using a QIAquick PCR Purification Kit (Qiagen) and stored at −20 °C until use. .. For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase.

    Polymerase Chain Reaction:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: .. PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ). .. Using the Cavendish PDS cDNA sequence (GenBank Accession ), primers were designed to amplify a short 5′ segment of the PDS gene from cv.

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: .. For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase. .. The mixture was then treated in a 15-μL NEB buffer 2 containing 5 mM DTT, ATP (50 pmol cold, premixed with 1.66 pmol [γ-32 P] ATP) and 10 U polynucleotide kinase.

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: .. We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India). .. All sequencing reactions were performed on ABI3100xl Automated system (Bangalore, India).

    Article Title: Contrasting life histories contribute to divergent patterns of genetic diversity and population connectivity in freshwater sculpin fishes
    Article Snippet: .. The amplified PCR products were purified enzymatically with Exonuclease I (New England BioLabs, Ipswich, MA, USA) and Shrimp Alkaline Phosphatase (New England BioLabs). .. The purified mtDNA fragments were subjected to direct sequencing using the internal forward LCCR and the same reverse H12Sr5 primers as in the PCR, and the BigDye Terminator 3.1 Cycle Sequencing Ready Reaction Kit in an ABI 3730xl automated DNA sequencer (Applied Biosystems).

    Article Title: A new Hermeuptychia (Lepidoptera, Nymphalidae, Satyrinae) is sympatric and synchronic with H. sosybius in southeast US coastal plains, while another new Hermeuptychia species - not hermes - inhabits south Texas and northeast Mexico
    Article Snippet: .. PCR reaction was cleaned up by enzymatic digestion for the whole barcode amplifications of DNA from freshly collected or alcohol preserved specimens and ID tag amplification of old specimens with 4 μl Shrimp Alkaline Phosphatase (20 U/μl) and 1 ul Exonuclease I (1 U/μl) from New England Biolabs. .. For older specimens that are barcoded in multiple segments, due to the frequent presence of primer dimers and other short non-specific PCR products, Agencourt Ampure XP beads or Invitrogen E-Gel® EX Agarose Gels (followed by Zymo gel DNA recovery kit) were used to select the DNA products of expected length.

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: .. The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs). .. The labeled PCR products were purified using the QIAquick nucleotide removal kit (QIAGEN, Valencia, CA) prior to hybridization.

    Article Title: DMSO increases mutation-scanning detection sensitivity in clinical samples using high resolution melting
    Article Snippet: .. The PCR products were digested by Exonuclease I and Shrimp Alkaline Phosphatase (New England Biolabs) and processed for Sanger sequencing at Eton Bioscience Inc. (Boston, MA, USA). .. To enable sequencing of short PCR amplicons, a 30-T tail was added to the 5′-end of the forward primer ( ).

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product. .. All amplified DNA was treated with DpnI to remove any remaining template and then cleaned using a Qiagen PCR purification kit.

    Article Title: Multiple Divergent Haplotypes Express Completely Distinct Sets of Class I MHC Genes in Zebrafish
    Article Snippet: .. Prior to sequencing, PCR reactions (5 μL aliquots) were first mixed with 3 units of Exonuclease (Affymetrix) and 0.3 units of Shrimp Alkaline Phosphatase (NEB) and incubated at 37°C for 30 minutes followed by inactivation at 80°C for 15 minutes. .. Sequences were analyzed using SeqMan (DNAstar) to identify specific SNPs and assign haplotypes ( ).

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: .. For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase. .. The above mixture was then treated in 15 μl of New England Biolabs buffer 3 with 5 m m DTT, ATP (50 pmol of cold, premixed with 1.66 pmol of [γ-32 P]ATP), and 5 units of T4 polynucleotide kinase.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: .. For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase. .. The above mixture was then treated in 15 μl of New England Biolabs buffer 3 with 5 m m DTT, ATP (50 pmol of cold, premixed with 1.66 pmol of [γ-32 P]ATP), and 5 units of T4 polynucleotide kinase.

    CRISPR:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: Paragraph title: Sequence analysis of CRISPR/Cas9 modified plants ... PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ).

    Chromatin Immunoprecipitation:

    Article Title: Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty
    Article Snippet: Paragraph title: Genome-wide ChIP assays ... Shortly, 10 µl of immunoprecipitated DNA were dephosphorylated using Shrimp Alkaline Phosphatase (NEB, 1 U) for 10 min at 37 °C.

    Plasmid Preparation:

    Article Title: Ethanol Stimulates Trehalose Production through a SpoT-DksA-AlgU-Dependent Pathway in Pseudomonas aeruginosa
    Article Snippet: .. The linearized plasmid was treated with shrimp alkaline phosphatase purchased from New England Biolabs and cleaned using a Qiagen PCR purification kit (Qiagen, Hilden, Germany). spoT fragment 1 was amplified from P. aeruginosa PA14 genomic DNA for a 1,369-bp PCR product. spoT fragment 2 was amplified from PA14 genomic DNA for a 1,468-bp PCR product. spoT fragment 3 was amplified from PA14 genomic DNA for a 1,268-bp PCR product. .. All amplified DNA was treated with DpnI to remove any remaining template and then cleaned using a Qiagen PCR purification kit.

    Software:

    Article Title: Contrasting life histories contribute to divergent patterns of genetic diversity and population connectivity in freshwater sculpin fishes
    Article Snippet: The amplified PCR products were purified enzymatically with Exonuclease I (New England BioLabs, Ipswich, MA, USA) and Shrimp Alkaline Phosphatase (New England BioLabs). .. The DNA sequences were edited using CHROMAS v2.01 computer software and aligned with Clustal Omega [ ], implemented in BioEdit v7.2.5 [ ], and finally verified by visual inspection.

    Article Title: The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation
    Article Snippet: Densitometric analysis was performed using ImageQuant TL software (GE Healthcare). .. The PCR products were dephosphorylated with shrimp alkaline phosphatase (New England Biolabs) and 5′-end labeled using [γ-32 P]dATP catalyzed by T4 polynucleotide kinase (New England Biolabs).

    Agarose Gel Electrophoresis:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: A sample (6 µL) of the PCR product was electrophoresed through a 1% TAE agarose gel to confirm the size of the amplicon. .. PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ).

    In Vitro:

    Article Title: Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty
    Article Snippet: Shortly, 10 µl of immunoprecipitated DNA were dephosphorylated using Shrimp Alkaline Phosphatase (NEB, 1 U) for 10 min at 37 °C. .. RNA was then synthesized by in vitro transcription using T7 RNA polymerase and the RNAMaxx high yield kit (Agilent) in an overnight reaction at 37 °C.

    Transgenic Assay:

    Article Title: Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
    Article Snippet: Twelve representative white colonies were selected for each transgenic line. .. PCR product (2 μL), 1 μL of 5X BrightDye® Sequencing buffer (Molecular Cloning Laboratories), 0.125 μL of Exonuclease I (NEB), 0.25 μL of Shrimp Alkaline Phosphatase (NEB) and 3.625 μL of ddH2 O were incubated at 37 °C for 45 min followed by inactivation at 80 °C for 15 min. Sequencing reactions were prepared containing 6 μL of enzyme treated PCR product, 1 µL of 5X BrightDye® Sequencing buffer, 0.6 µL of BrightDye® Terminator, 3.2 pmol of M13-F primer and ddH2 , Kearse et al. ).

    Immunoprecipitation:

    Article Title: Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty
    Article Snippet: .. Shortly, 10 µl of immunoprecipitated DNA were dephosphorylated using Shrimp Alkaline Phosphatase (NEB, 1 U) for 10 min at 37 °C. .. Thereafter, thymidine residues were added to the DNA ends using Terminal Transferase (NEB, 20 U), in a T-tailing reaction (20 min at 37 °C) that utilized 5 μM dTTP and 5 μM ddCTP.

    Staining:

    Article Title: Contrasting life histories contribute to divergent patterns of genetic diversity and population connectivity in freshwater sculpin fishes
    Article Snippet: PCR products were checked on 2% agarose gels stained with RedSafe (iNtRon Biotechnology, Daejeon, Korea). .. The amplified PCR products were purified enzymatically with Exonuclease I (New England BioLabs, Ipswich, MA, USA) and Shrimp Alkaline Phosphatase (New England BioLabs).

    Fluorescence In Situ Hybridization:

    Article Title: Multiple Divergent Haplotypes Express Completely Distinct Sets of Class I MHC Genes in Zebrafish
    Article Snippet: In addition, PCR bias is likely to be observed using the psmb8a primer set with DNA from heterozygous haplotype E fish, displaying lower signals for the haplotype E specific SNPs compared with the other psmb8a allelic sequences. .. Prior to sequencing, PCR reactions (5 μL aliquots) were first mixed with 3 units of Exonuclease (Affymetrix) and 0.3 units of Shrimp Alkaline Phosphatase (NEB) and incubated at 37°C for 30 minutes followed by inactivation at 80°C for 15 minutes.

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    New England Biolabs shrimp alkaline phosphatase rsap
    Shrimp Alkaline Phosphatase Rsap, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase rsap/product/New England Biolabs
    Average 90 stars, based on 156 article reviews
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    shrimp alkaline phosphatase rsap - by Bioz Stars, 2020-01
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