shrimp alkaline phosphatase  (New England Biolabs)


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  • 99
    Name:
    Shrimp Alkaline Phosphatase rSAP
    Description:
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    Catalog Number:
    m0371l
    Price:
    248
    Size:
    2 500 units
    Category:
    Alkaline Phosphatases
    Buy from Supplier


    Structured Review

    New England Biolabs shrimp alkaline phosphatase
    Shrimp Alkaline Phosphatase rSAP
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    https://www.bioz.com/result/shrimp alkaline phosphatase/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: .. For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase. .. The mixture was then treated in a 15-μL NEB buffer 2 containing 5 mM DTT, ATP (50 pmol cold, premixed with 1.66 pmol [γ-32 P] ATP) and 10 U polynucleotide kinase.

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: .. We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India). .. All sequencing reactions were performed on ABI3100xl Automated system (Bangalore, India).

    Incubation:

    Article Title: Telomere length and telomerase activity in T cells are biomarkers of high‐performing centenarians, et al. Telomere length and telomerase activity in T cells are biomarkers of high‐performing centenarians
    Article Snippet: .. Briefly, 50 ng of genomic DNA was added to a final volume of 20 µl of ligation buffer containing 1,000 units of T4 DNA ligase (New England Biolabs), 1× Cut Smart Buffer (New England Biolabs), 1 mM ATP, and 1 nM of TeSLA telorettes (TeSLA Telo 1–6) (Lai et al., ) and incubated at 35°C for 16 hr followed by heat inactivation at 65°C for 10 min. After ligation, genomic DNA was digested using a set of restriction enzymes (2 units each of CviAII, Bfa I, Nde I, and Mse I, New England Biolabs) as reported (Lai et al., ) and then treated with 1 unit of shrimp alkaline phosphatase (rSAP; New England Biolabs) at 37°C for 60 min in a final volume of 50 µl. .. This mixture was subsequently heat inactivated at 80°C for 20 min, and 10 µl of sample was added to 10 µl of adapter ligation mix (1 μM AT adapter, 1 μM TA adapter, 1 mM ATP, 1× Cut Smart Buffer, and 2,000 units of T4 DNA Ligase) and incubated at 16°C for 16 hr.

    Article Title: Cell cycle dynamics of lamina associated DNA
    Article Snippet: .. To do so, up to 500 ng of gDNA was incubated in 10 µL H2 O with 1x CutSmart buffer and 0.5 U rSAP (New England BioLabs #M0371L) for 1 hour at 37°C, followed by heat inactivation for 10 minutes at 65°C. .. Next, 10 U DpnI (New England BioLabs #R0176L) was added and sample processing continued as described [ ].

    Ligation:

    Article Title: Telomere length and telomerase activity in T cells are biomarkers of high‐performing centenarians, et al. Telomere length and telomerase activity in T cells are biomarkers of high‐performing centenarians
    Article Snippet: .. Briefly, 50 ng of genomic DNA was added to a final volume of 20 µl of ligation buffer containing 1,000 units of T4 DNA ligase (New England Biolabs), 1× Cut Smart Buffer (New England Biolabs), 1 mM ATP, and 1 nM of TeSLA telorettes (TeSLA Telo 1–6) (Lai et al., ) and incubated at 35°C for 16 hr followed by heat inactivation at 65°C for 10 min. After ligation, genomic DNA was digested using a set of restriction enzymes (2 units each of CviAII, Bfa I, Nde I, and Mse I, New England Biolabs) as reported (Lai et al., ) and then treated with 1 unit of shrimp alkaline phosphatase (rSAP; New England Biolabs) at 37°C for 60 min in a final volume of 50 µl. .. This mixture was subsequently heat inactivated at 80°C for 20 min, and 10 µl of sample was added to 10 µl of adapter ligation mix (1 μM AT adapter, 1 μM TA adapter, 1 mM ATP, 1× Cut Smart Buffer, and 2,000 units of T4 DNA Ligase) and incubated at 16°C for 16 hr.

    Purification:

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: .. We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India). .. All sequencing reactions were performed on ABI3100xl Automated system (Bangalore, India).

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  • 90
    New England Biolabs shrimp alkaline phosphatase rsap
    Shrimp Alkaline Phosphatase Rsap, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase rsap/product/New England Biolabs
    Average 90 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase rsap - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    New England Biolabs shrimp alkaline phospatase
    Shrimp Alkaline Phospatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phospatase/product/New England Biolabs
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phospatase - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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