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Illumina Inc short oligos
Short Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/short oligos/product/Illumina Inc
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
short oligos - by Bioz Stars, 2020-04
91/100 stars

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Multiplexing:

Article Title: The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Article Snippet: The amplified DNA was ligated with two standard adaptors that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flow cell. .. These adaptors included 7 bp unique index sequences to enable multiplexing of more than one sample in the same run.

Flow Cytometry:

Article Title: The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Article Snippet: .. The amplified DNA was ligated with two standard adaptors that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flow cell. .. These adaptors included 7 bp unique index sequences to enable multiplexing of more than one sample in the same run.

Amplification:

Article Title: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
Article Snippet: The second-round PCR (second PCR; ) used the first PCR products as a template and amplified the region using primers 5′-AATGATACGGCGACCACCGAGATCTACAXXXXXXXXACACTCTTTCCC TACACGACGCTCTTCCGATCT-3′ (forward) and 5′-CAAGCAGAAGACGGCATACGAGATXXXXXX XXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′ (reverse). .. The octo-X segments represent dual-index sequences (40 unique indices in total; A501–508, A701–712 and D501–508, D701–D712; Illumina); the 5′-end sequences are adapters that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flowcell; and the 3′-end sequences are priming sites for the MiSeq sequencing.

Article Title: The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Article Snippet: .. The amplified DNA was ligated with two standard adaptors that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flow cell. .. These adaptors included 7 bp unique index sequences to enable multiplexing of more than one sample in the same run.

Synthesized:

Article Title: The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Article Snippet: The amplified DNA was ligated with two standard adaptors that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flow cell. .. A total of 36 barcode-primer combinations were synthesized and used in two runs (since we had a total of 70 samples).

Purification:

Article Title: The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Article Snippet: The amplified DNA was ligated with two standard adaptors that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flow cell. .. Purified PCR products (with adaptor and barcode sequences) from each run were pooled accordingly to form two separate sequencing libraries.

Sequencing:

Article Title: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
Article Snippet: .. The octo-X segments represent dual-index sequences (40 unique indices in total; A501–508, A701–712 and D501–508, D701–D712; Illumina); the 5′-end sequences are adapters that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flowcell; and the 3′-end sequences are priming sites for the MiSeq sequencing. .. The first PCR product was diluted 10 times using Milli-Q water and used as a template for the second PCR.

Article Title: The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Article Snippet: Paragraph title: Library construction and sequencing ... The amplified DNA was ligated with two standard adaptors that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flow cell.

Concentration Assay:

Article Title: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
Article Snippet: When the first PCR was multiplexed (simultaneous use of multiple primer pairs), the final concentration of each primer was 0.3 μM and sterile distilled H2 O was added up to the total reaction volume of 12.0 μl. .. The octo-X segments represent dual-index sequences (40 unique indices in total; A501–508, A701–712 and D501–508, D701–D712; Illumina); the 5′-end sequences are adapters that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flowcell; and the 3′-end sequences are priming sites for the MiSeq sequencing.

Polymerase Chain Reaction:

Article Title: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
Article Snippet: The second-round PCR (second PCR; ) used the first PCR products as a template and amplified the region using primers 5′-AATGATACGGCGACCACCGAGATCTACAXXXXXXXXACACTCTTTCCC TACACGACGCTCTTCCGATCT-3′ (forward) and 5′-CAAGCAGAAGACGGCATACGAGATXXXXXX XXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′ (reverse). .. The octo-X segments represent dual-index sequences (40 unique indices in total; A501–508, A701–712 and D501–508, D701–D712; Illumina); the 5′-end sequences are adapters that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flowcell; and the 3′-end sequences are priming sites for the MiSeq sequencing.

Article Title: The utility of DNA metabarcoding for studying the response of arthropod diversity and composition to land-use change in the tropics
Article Snippet: Library construction and sequencing Amplification was carried out as described above, and PCR products were quantified using Qubit 2.0 Fluorometer (dsDNA HS Assay, Life Technologies). .. The amplified DNA was ligated with two standard adaptors that allow the final product to bind or hybridize to short oligos on the surface of the Illumina flow cell.

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