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Shimadzu Corporation shimadzu lc ms spectrometer
Shimadzu Lc Ms Spectrometer, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 2 article reviews
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High Performance Liquid Chromatography:

Article Title: A focused library synthesis and cytotoxicity of quinones derived from the natural product bolinaquinone
Article Snippet: .. Electrospray mass spectra were recorded using 10% DMSO/H2 O or HPLC-grade methanol or acetonitrile as carrier solvents on a Shimadzu LC-MS spectrometer, or an Agilent Technologies 1260 Infinity UPLC system with a 6120 Quadrupole LC/MS in electrospray ionization (ESI) positive and negative modes. .. TLC was performed on Merck silica gel 60 F254 pre-coated aluminium plates with a thickness of 0.2 mm.

Liquid Chromatography with Mass Spectroscopy:

Article Title: A focused library synthesis and cytotoxicity of quinones derived from the natural product bolinaquinone
Article Snippet: .. Electrospray mass spectra were recorded using 10% DMSO/H2 O or HPLC-grade methanol or acetonitrile as carrier solvents on a Shimadzu LC-MS spectrometer, or an Agilent Technologies 1260 Infinity UPLC system with a 6120 Quadrupole LC/MS in electrospray ionization (ESI) positive and negative modes. .. TLC was performed on Merck silica gel 60 F254 pre-coated aluminium plates with a thickness of 0.2 mm.

Article Title: Evaluation of Nitrobenzyl Derivatives of Camptothecin as Anti-Cancer Agents and Potential Hypoxia Targeting Prodrugs
Article Snippet: .. Liquid chromatography-mass spectrometry (LC-MS) analyses were performed on a Shimadzu LC-MS spectrometer, Kyoto, Japan. ..

Chromatography:

Article Title: Evaluation of Nitrobenzyl Derivatives of Camptothecin as Anti-Cancer Agents and Potential Hypoxia Targeting Prodrugs
Article Snippet: .. Liquid chromatography-mass spectrometry (LC-MS) analyses were performed on a Shimadzu LC-MS spectrometer, Kyoto, Japan. ..

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    Shimadzu Corporation lc it tof ms
    <t>LC-IT-TOF</t> MS chromatograms of <t>Acori</t> Graminei Rhizoma. LC chromatogram (a) and total ion chromatogram in positive ion mode (b). β -asarone (1).
    Lc It Tof Ms, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Shimadzu Corporation mito chmac
    Intracellular accumulation of <t>Mito-ChM</t> in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM <t>Mito-ChMAc.</t> ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.
    Mito Chmac, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Shimadzu Corporation direct lc ms ms analysis
    Enhanced product ion <t>(EPI)-MS</t> spectra of the two insulin-SF conjugates. MS 2 product ion spectra of (A) 6.46 min and (B) 7.08 min retention time peaks from <t>LC-MS</t> <t>analysis</t> of human plasma incubated with bovine insulin and 50 µM SF for 4 h at 37°C. In (A) and (B) m/z 1183.9 corresponds to insulin-SF MH 5 5+ and in (A) m/z 235.0 corresponds to Gly-SF, the N-terminal amino acid of insulin A chain and in (B) m/z 325.2 corresponds to Phe-SF, the N-terminal amino acid of insulin B chain.
    Direct Lc Ms Ms Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Shimadzu Corporation uplc pad analysis hplc pad analysis
    The <t>UPLC-PAD</t> chromatograms of the Yejuhua capsule and its three ingredients by UPLC-PAD analysis. The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients. SFEC: supercritical-carbon dioxide fluid extraction of Chrysanthemi Indici Flos (a), PO: Patchouli Oil (b), ZTO: Zedoary Turmeric Oil (c), and YJH: Yejuhua capsule (d). The structures of authentic standard compounds used in the UPLC-PAD analysis (e).
    Uplc Pad Analysis Hplc Pad Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LC-IT-TOF MS chromatograms of Acori Graminei Rhizoma. LC chromatogram (a) and total ion chromatogram in positive ion mode (b). β -asarone (1).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Water Extract of Acori Graminei Rhizoma Attenuates Features of Rheumatoid Arthritis in DBA/1 Mice

    doi: 10.1155/2019/3637453

    Figure Lengend Snippet: LC-IT-TOF MS chromatograms of Acori Graminei Rhizoma. LC chromatogram (a) and total ion chromatogram in positive ion mode (b). β -asarone (1).

    Article Snippet: LC-IT-TOF-MS Conditions of Acori Graminei Rhizoma Extract WAG was analyzed by ESI-mass spectra which were obtained on a LC-IT-TOF mass spectrometer (Shimadzu, Japan).

    Techniques: Mass Spectrometry

    Intracellular accumulation of Mito-ChM in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM Mito-ChMAc. ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.

    Journal: BMC Cancer

    Article Title: Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death

    doi: 10.1186/1471-2407-13-285

    Figure Lengend Snippet: Intracellular accumulation of Mito-ChM in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM Mito-ChMAc. ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.

    Article Snippet: The simultaneous quantification of Mito-ChM and Mito-ChMAc in the extracts was performed using the UHPLC system (Shimadzu Nexera) coupled to an MS/MS detector (Shimadzu 8030).

    Techniques: Multiple Displacement Amplification, High Performance Liquid Chromatography, Concentration Assay, Incubation, Mass Spectrometry

    Synergistic cytotoxicity and anti-proliferative effects of Mito-ChM and 2-DG. ( A ) Representative pictures of the colonies formed. MCF-7 and MCF-10A cells were treated with 2-DG only ( upper ) or 2-DG in the presence of Mito-ChM (1 μM) ( lower ) for 4 h and the colonies formed were counted. ( B ) The survival fraction was calculated under the same conditions as in ( A ). ( C , D ) Cytotoxic effects of the combination of Mito-ChM and 2-DG. MCF-7 (main panels) and MCF-10A (inserts) cells were treated with 2-DG alone (at the indicated concentrations), 2-DG in the presence of 1 μM Mito-ChM ( C ) or 1 μM Mito-ChMAc ( D ) for 24 h and cell death was monitored in real time by Sytox Green staining. Data shown are the mean ± SEM. n = 4-6.

    Journal: BMC Cancer

    Article Title: Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death

    doi: 10.1186/1471-2407-13-285

    Figure Lengend Snippet: Synergistic cytotoxicity and anti-proliferative effects of Mito-ChM and 2-DG. ( A ) Representative pictures of the colonies formed. MCF-7 and MCF-10A cells were treated with 2-DG only ( upper ) or 2-DG in the presence of Mito-ChM (1 μM) ( lower ) for 4 h and the colonies formed were counted. ( B ) The survival fraction was calculated under the same conditions as in ( A ). ( C , D ) Cytotoxic effects of the combination of Mito-ChM and 2-DG. MCF-7 (main panels) and MCF-10A (inserts) cells were treated with 2-DG alone (at the indicated concentrations), 2-DG in the presence of 1 μM Mito-ChM ( C ) or 1 μM Mito-ChMAc ( D ) for 24 h and cell death was monitored in real time by Sytox Green staining. Data shown are the mean ± SEM. n = 4-6.

    Article Snippet: The simultaneous quantification of Mito-ChM and Mito-ChMAc in the extracts was performed using the UHPLC system (Shimadzu Nexera) coupled to an MS/MS detector (Shimadzu 8030).

    Techniques: Staining

    Enhanced product ion (EPI)-MS spectra of the two insulin-SF conjugates. MS 2 product ion spectra of (A) 6.46 min and (B) 7.08 min retention time peaks from LC-MS analysis of human plasma incubated with bovine insulin and 50 µM SF for 4 h at 37°C. In (A) and (B) m/z 1183.9 corresponds to insulin-SF MH 5 5+ and in (A) m/z 235.0 corresponds to Gly-SF, the N-terminal amino acid of insulin A chain and in (B) m/z 325.2 corresponds to Phe-SF, the N-terminal amino acid of insulin B chain.

    Journal: PLoS ONE

    Article Title: Broccoli Consumption Interacts with GSTM1 to Perturb Oncogenic Signalling Pathways in the Prostate

    doi: 10.1371/journal.pone.0002568

    Figure Lengend Snippet: Enhanced product ion (EPI)-MS spectra of the two insulin-SF conjugates. MS 2 product ion spectra of (A) 6.46 min and (B) 7.08 min retention time peaks from LC-MS analysis of human plasma incubated with bovine insulin and 50 µM SF for 4 h at 37°C. In (A) and (B) m/z 1183.9 corresponds to insulin-SF MH 5 5+ and in (A) m/z 235.0 corresponds to Gly-SF, the N-terminal amino acid of insulin A chain and in (B) m/z 325.2 corresponds to Phe-SF, the N-terminal amino acid of insulin B chain.

    Article Snippet: Direct LC-MS/MS analysis of peptides incubated with isothiocyanates The LC system used was a Shimadzu series 10AD VP (Shimadzu, http://www.shimadzu.com/ ).

    Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Incubation

    The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients by UPLC-PAD analysis. The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients. SFEC: supercritical-carbon dioxide fluid extraction of Chrysanthemi Indici Flos (a), PO: Patchouli Oil (b), ZTO: Zedoary Turmeric Oil (c), and YJH: Yejuhua capsule (d). The structures of authentic standard compounds used in the UPLC-PAD analysis (e).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: A Standardized Traditional Chinese Medicine Preparation Named Yejuhua Capsule Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Downregulating Toll-Like Receptor 4/Nuclear Factor-κB

    doi: 10.1155/2015/264612

    Figure Lengend Snippet: The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients by UPLC-PAD analysis. The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients. SFEC: supercritical-carbon dioxide fluid extraction of Chrysanthemi Indici Flos (a), PO: Patchouli Oil (b), ZTO: Zedoary Turmeric Oil (c), and YJH: Yejuhua capsule (d). The structures of authentic standard compounds used in the UPLC-PAD analysis (e).

    Article Snippet: Apparatus and UPLC-PAD Analysis HPLC-PAD analysis was carried out on a Shimadzu LC-20A HPLC system consisting of a SPD-M20A PDA detector, a LC-20AT pump, a SIL-20AC automatic sampler, a CTO-20A thermostatic column compartment, and a Shimadzu LC-20A software (Shimadzu, Kyoto, Japan).

    Techniques:

    The GC-MS and UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule. The GC-MS fingerprinting chromatograms of 10 batches of Yejuhua capsule (a) and the UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule (b). YJH131-YJH1310: batch of Yejuhua capsule sample from number 1 to number 10.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: A Standardized Traditional Chinese Medicine Preparation Named Yejuhua Capsule Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Downregulating Toll-Like Receptor 4/Nuclear Factor-κB

    doi: 10.1155/2015/264612

    Figure Lengend Snippet: The GC-MS and UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule. The GC-MS fingerprinting chromatograms of 10 batches of Yejuhua capsule (a) and the UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule (b). YJH131-YJH1310: batch of Yejuhua capsule sample from number 1 to number 10.

    Article Snippet: Apparatus and UPLC-PAD Analysis HPLC-PAD analysis was carried out on a Shimadzu LC-20A HPLC system consisting of a SPD-M20A PDA detector, a LC-20AT pump, a SIL-20AC automatic sampler, a CTO-20A thermostatic column compartment, and a Shimadzu LC-20A software (Shimadzu, Kyoto, Japan).

    Techniques: Gas Chromatography-Mass Spectrometry