sheep kidney na k atpase  (Millipore)


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    Structured Review

    Millipore sheep kidney na k atpase
    Stable expression of wild-type and mutant forms of rat α -1 subunit of Na+ /K+ <t>-ATPase</t> in OK cells
    Sheep Kidney Na K Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep kidney na k atpase/product/Millipore
    Average 85 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    sheep kidney na k atpase - by Bioz Stars, 2020-09
    85/100 stars

    Images

    1) Product Images from "Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase"

    Article Title: Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase

    Journal: The Biochemical journal

    doi: 10.1042/BJ20111398

    Stable expression of wild-type and mutant forms of rat α -1 subunit of Na+ /K+ -ATPase in OK cells
    Figure Legend Snippet: Stable expression of wild-type and mutant forms of rat α -1 subunit of Na+ /K+ -ATPase in OK cells

    Techniques Used: Expressing, Mutagenesis

    Relative effect of Ang II on the elution of plasma membrane protein containing Populations #1, #2 and #3 of Na + /K + -ATPase from rat α -1.wild-type (A), α -1.S11A/S18A (B) and α -1.S938A (C) cells
    Figure Legend Snippet: Relative effect of Ang II on the elution of plasma membrane protein containing Populations #1, #2 and #3 of Na + /K + -ATPase from rat α -1.wild-type (A), α -1.S11A/S18A (B) and α -1.S938A (C) cells

    Techniques Used:

    Three populations of Na+ /K+ -ATPase in plasma membranes of α -1.S11A/S18A and α -1.S938A cells
    Figure Legend Snippet: Three populations of Na+ /K+ -ATPase in plasma membranes of α -1.S11A/S18A and α -1.S938A cells

    Techniques Used:

    Phosphorylation of rat kidney Na + /K + -ATPase at Ser 18
    Figure Legend Snippet: Phosphorylation of rat kidney Na + /K + -ATPase at Ser 18

    Techniques Used:

    Phosphorylation of rat kidney Na + /K + -ATPase at Ser 11
    Figure Legend Snippet: Phosphorylation of rat kidney Na + /K + -ATPase at Ser 11

    Techniques Used:

    Phosphorylation of rat kidney Na + /K + -ATPase at Ser 938
    Figure Legend Snippet: Phosphorylation of rat kidney Na + /K + -ATPase at Ser 938

    Techniques Used:

    Elution from digoxin-affinity columns of plasma membrane protein containing Na + /K + -ATPase Populations #1 (fractions 1–9), #2 (fractions 10–18) and #3 (fractions 19–22) from control and Ang II-treated α -1.S11A/S18A cells
    Figure Legend Snippet: Elution from digoxin-affinity columns of plasma membrane protein containing Na + /K + -ATPase Populations #1 (fractions 1–9), #2 (fractions 10–18) and #3 (fractions 19–22) from control and Ang II-treated α -1.S11A/S18A cells

    Techniques Used:

    2) Product Images from "Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase"

    Article Title: Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase

    Journal: The Biochemical journal

    doi: 10.1042/BJ20111398

    Stable expression of wild-type and mutant forms of rat α -1 subunit of Na+ /K+ -ATPase in OK cells
    Figure Legend Snippet: Stable expression of wild-type and mutant forms of rat α -1 subunit of Na+ /K+ -ATPase in OK cells

    Techniques Used: Expressing, Mutagenesis

    Relative effect of Ang II on the elution of plasma membrane protein containing Populations #1, #2 and #3 of Na + /K + -ATPase from rat α -1.wild-type (A), α -1.S11A/S18A (B) and α -1.S938A (C) cells
    Figure Legend Snippet: Relative effect of Ang II on the elution of plasma membrane protein containing Populations #1, #2 and #3 of Na + /K + -ATPase from rat α -1.wild-type (A), α -1.S11A/S18A (B) and α -1.S938A (C) cells

    Techniques Used:

    Three populations of Na+ /K+ -ATPase in plasma membranes of α -1.S11A/S18A and α -1.S938A cells
    Figure Legend Snippet: Three populations of Na+ /K+ -ATPase in plasma membranes of α -1.S11A/S18A and α -1.S938A cells

    Techniques Used:

    Phosphorylation of rat kidney Na + /K + -ATPase at Ser 18
    Figure Legend Snippet: Phosphorylation of rat kidney Na + /K + -ATPase at Ser 18

    Techniques Used:

    Phosphorylation of rat kidney Na + /K + -ATPase at Ser 11
    Figure Legend Snippet: Phosphorylation of rat kidney Na + /K + -ATPase at Ser 11

    Techniques Used:

    Phosphorylation of rat kidney Na + /K + -ATPase at Ser 938
    Figure Legend Snippet: Phosphorylation of rat kidney Na + /K + -ATPase at Ser 938

    Techniques Used:

    Elution from digoxin-affinity columns of plasma membrane protein containing Na + /K + -ATPase Populations #1 (fractions 1–9), #2 (fractions 10–18) and #3 (fractions 19–22) from control and Ang II-treated α -1.S11A/S18A cells
    Figure Legend Snippet: Elution from digoxin-affinity columns of plasma membrane protein containing Na + /K + -ATPase Populations #1 (fractions 1–9), #2 (fractions 10–18) and #3 (fractions 19–22) from control and Ang II-treated α -1.S11A/S18A cells

    Techniques Used:

    3) Product Images from "The N-terminus of the serum- and glucocorticoid-inducible kinase Sgk1 specifies mitochondrial localization and rapid turnover"

    Article Title: The N-terminus of the serum- and glucocorticoid-inducible kinase Sgk1 specifies mitochondrial localization and rapid turnover

    Journal: Biochemical Journal

    doi: 10.1042/BJ20060386

    Sgk1 associates with the OMM ( A ) Membrane association of imported radiolabelled Sgk1 was assessed by preparation of mitochondrial (mitos) soluble (s/n) and membrane (mem) fractions. Radiolabelled proteins were visualized by autoradiography (AR). The peripheral membrane protein cytochrome c (CytC) was released by Na 2 CO 3 treatment and visualized by Western blot (WB, lower panel). Star symbol: this signal sometimes occurred after in vitro translation and is most likely a degradation product (also in B ). ( B ) After import of in vitro -translated radiolabelled Sgk1, mitochondria were treated with 200 μg/ml proteinase K (PK) in the presence or absence of Triton X-100 or left untreated. Sgk1 was subject to complete digestion, indicating a localization to the cytoplasmic face of the OMM. ivt; an aliquot of the in vitro translation product. ( C ) Aliquots of the protease protection assay were subjected to Western-blot analyses with antibodies directed against the matrix protein Hsp60, and the intermembrane space proteins Smac/DIABLO and cytochrome c ], and therefore served as loading control. Hsp60 and Smac/DIABLO remain intact in the absence of Triton X-100, demonstrating the integrity of the organelles. ( D ) The capability of the preparation to import proteins correctly is shown by the import of in vitro -translated fumarate hydratase (FH) in parallel. Imported fumarate hydratase migrates slightly faster than the in vitro translation product (ivt) because of the removal of the signal peptide by the processing peptidase after import into the matrix.
    Figure Legend Snippet: Sgk1 associates with the OMM ( A ) Membrane association of imported radiolabelled Sgk1 was assessed by preparation of mitochondrial (mitos) soluble (s/n) and membrane (mem) fractions. Radiolabelled proteins were visualized by autoradiography (AR). The peripheral membrane protein cytochrome c (CytC) was released by Na 2 CO 3 treatment and visualized by Western blot (WB, lower panel). Star symbol: this signal sometimes occurred after in vitro translation and is most likely a degradation product (also in B ). ( B ) After import of in vitro -translated radiolabelled Sgk1, mitochondria were treated with 200 μg/ml proteinase K (PK) in the presence or absence of Triton X-100 or left untreated. Sgk1 was subject to complete digestion, indicating a localization to the cytoplasmic face of the OMM. ivt; an aliquot of the in vitro translation product. ( C ) Aliquots of the protease protection assay were subjected to Western-blot analyses with antibodies directed against the matrix protein Hsp60, and the intermembrane space proteins Smac/DIABLO and cytochrome c ], and therefore served as loading control. Hsp60 and Smac/DIABLO remain intact in the absence of Triton X-100, demonstrating the integrity of the organelles. ( D ) The capability of the preparation to import proteins correctly is shown by the import of in vitro -translated fumarate hydratase (FH) in parallel. Imported fumarate hydratase migrates slightly faster than the in vitro translation product (ivt) because of the removal of the signal peptide by the processing peptidase after import into the matrix.

    Techniques Used: Autoradiography, Western Blot, In Vitro

    Amino acids 17–33 target Sgk1 to mitochondria in HEK-293 cells HEK-293 cells were transfected with the mitochondrial marker cytochrome c oxidase subunit VIII coupled with DsRedII and either full-length Sgk1–GFP ( A ) or Sgk1–GFP deletion variants ( B – J ). Cells were grown on coverslips and formaldehyde-fixed, and nuclei were stained with DAPI. Left panels show GFP fluorescence signals (green), middle panels show DAPI (blue) and mitochondrial staining (red) and right panels show merged images. Yellow colour indicates co-localization.
    Figure Legend Snippet: Amino acids 17–33 target Sgk1 to mitochondria in HEK-293 cells HEK-293 cells were transfected with the mitochondrial marker cytochrome c oxidase subunit VIII coupled with DsRedII and either full-length Sgk1–GFP ( A ) or Sgk1–GFP deletion variants ( B – J ). Cells were grown on coverslips and formaldehyde-fixed, and nuclei were stained with DAPI. Left panels show GFP fluorescence signals (green), middle panels show DAPI (blue) and mitochondrial staining (red) and right panels show merged images. Yellow colour indicates co-localization.

    Techniques Used: Transfection, Marker, Staining, Fluorescence

    Sgk1 localizes to mitochondria in vivo The distribution of the Sgk1 protein in subcellular fractions from liver tissue was analysed 4 h after intraperitoneal administration of 100 μg/kg body weight dexamethasone. To be able to identify Sgk1 signals, Sgk1 knockouts (−/−) and their wild-type littermates were analysed in parallel. Signals were obtained predominantly from the mitochondrial fraction and only weakly from the cytosolic and microsomal fractions. Lower panels show immunoblots with antibodies against the indicated organelle markers to demonstrate purity of the fractions. Protein (20 μg/lane) was loaded for detection of Sgk1 and 10 μg of protein was loaded for detection of organelle markers. For the detection of Sgk1, we employed the antibody obtained from Sigma. PM, plasma membrane.
    Figure Legend Snippet: Sgk1 localizes to mitochondria in vivo The distribution of the Sgk1 protein in subcellular fractions from liver tissue was analysed 4 h after intraperitoneal administration of 100 μg/kg body weight dexamethasone. To be able to identify Sgk1 signals, Sgk1 knockouts (−/−) and their wild-type littermates were analysed in parallel. Signals were obtained predominantly from the mitochondrial fraction and only weakly from the cytosolic and microsomal fractions. Lower panels show immunoblots with antibodies against the indicated organelle markers to demonstrate purity of the fractions. Protein (20 μg/lane) was loaded for detection of Sgk1 and 10 μg of protein was loaded for detection of organelle markers. For the detection of Sgk1, we employed the antibody obtained from Sigma. PM, plasma membrane.

    Techniques Used: In Vivo, Western Blot

    Sgk1 associates with purified mitochondria in a time-dependent manner Radiolabelled in vitro -translated full-length Sgk1 and Sgk1 Δ1–59 were exposed to highly purified mitochondria from mouse kidneys for the indicated times. Mitochondria were washed, lysed and separated by PAGE. Full-length Sgk1 is imported in a time-dependent manner, whereas the truncated variant is not. Lower panels show aliquots from the supernatants of the import reaction. Signals were visualized by fluorography.
    Figure Legend Snippet: Sgk1 associates with purified mitochondria in a time-dependent manner Radiolabelled in vitro -translated full-length Sgk1 and Sgk1 Δ1–59 were exposed to highly purified mitochondria from mouse kidneys for the indicated times. Mitochondria were washed, lysed and separated by PAGE. Full-length Sgk1 is imported in a time-dependent manner, whereas the truncated variant is not. Lower panels show aliquots from the supernatants of the import reaction. Signals were visualized by fluorography.

    Techniques Used: Purification, In Vitro, Polyacrylamide Gel Electrophoresis, Variant Assay

    Rapid turnover of Sgk1 is determined by amino acids 17–47 Sgk1–GFP fusion protein variants or GFP alone were transfected into HEK-293 cells and subjected to pulse–chase analysis. After a pulse of 10 min, proteins were chased for 0 min, 15 min, 1 h or 4 h and the respective fusion proteins were recovered by immunoprecipitation with an anti-GFP antiserum. All variants containing amino acids 17–47 were rapidly degraded.
    Figure Legend Snippet: Rapid turnover of Sgk1 is determined by amino acids 17–47 Sgk1–GFP fusion protein variants or GFP alone were transfected into HEK-293 cells and subjected to pulse–chase analysis. After a pulse of 10 min, proteins were chased for 0 min, 15 min, 1 h or 4 h and the respective fusion proteins were recovered by immunoprecipitation with an anti-GFP antiserum. All variants containing amino acids 17–47 were rapidly degraded.

    Techniques Used: Transfection, Pulse Chase, Immunoprecipitation

    Amino acid sequence and hydropathy of the Sgk1 N-terminus ( A ) The N-terminal 60 amino acids of murine Sgk1. Residues are shown in the one-letter code. Starting amino acids for the constructs used in the present study are in boldface; in some constructs, Tyr 47 was replaced by methionine. Grey box highlights a predicted mitochondrial targeting sequence, and arrowhead indicates a potential cleavage site for the mitochondrial processing peptidase at amino acid position 33. The black bar indicates a potential transmembrane segment as predicted by the Kyte-Doolittle analysis. ( B ) Kyte-Doolittle hydropathy plot of Sgk1. The N-terminus of Sgk1 contains a potential transmembrane (TM) segment.
    Figure Legend Snippet: Amino acid sequence and hydropathy of the Sgk1 N-terminus ( A ) The N-terminal 60 amino acids of murine Sgk1. Residues are shown in the one-letter code. Starting amino acids for the constructs used in the present study are in boldface; in some constructs, Tyr 47 was replaced by methionine. Grey box highlights a predicted mitochondrial targeting sequence, and arrowhead indicates a potential cleavage site for the mitochondrial processing peptidase at amino acid position 33. The black bar indicates a potential transmembrane segment as predicted by the Kyte-Doolittle analysis. ( B ) Kyte-Doolittle hydropathy plot of Sgk1. The N-terminus of Sgk1 contains a potential transmembrane (TM) segment.

    Techniques Used: Sequencing, Construct

    4) Product Images from "Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase"

    Article Title: Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase

    Journal: The Biochemical journal

    doi: 10.1042/BJ20111398

    Effect of Ang II on the fractional amount of plasma membrane protein that remained on the digoxin-affinity columns as the columns were eluted with Solution #1, #2 and SDS for rat  α -1.wild-type (A),  α -1.S11A/S18A cells (B) and  α
    Figure Legend Snippet: Effect of Ang II on the fractional amount of plasma membrane protein that remained on the digoxin-affinity columns as the columns were eluted with Solution #1, #2 and SDS for rat α -1.wild-type (A), α -1.S11A/S18A cells (B) and α

    Techniques Used:

    Stable expression of wild-type and mutant forms of rat  α -1 subunit of Na+ /K+ -ATPase in OK cells
    Figure Legend Snippet: Stable expression of wild-type and mutant forms of rat α -1 subunit of Na+ /K+ -ATPase in OK cells

    Techniques Used: Expressing, Mutagenesis

    Relative effect of Ang II on the elution of plasma membrane protein containing Populations #1, #2 and #3 of Na + /K + -ATPase from rat  α -1.wild-type (A),  α -1.S11A/S18A (B) and  α -1.S938A (C) cells
    Figure Legend Snippet: Relative effect of Ang II on the elution of plasma membrane protein containing Populations #1, #2 and #3 of Na + /K + -ATPase from rat α -1.wild-type (A), α -1.S11A/S18A (B) and α -1.S938A (C) cells

    Techniques Used:

    Three populations of Na+ /K+ -ATPase in plasma membranes of  α -1.S11A/S18A and  α -1.S938A cells
    Figure Legend Snippet: Three populations of Na+ /K+ -ATPase in plasma membranes of α -1.S11A/S18A and α -1.S938A cells

    Techniques Used:

    Elution from digoxin-affinity columns of plasma membrane protein containing Na + /K + -ATPase Populations #1 (fractions 1–9), #2 (fractions 10–18) and #3 (fractions 19–22) from control and Ang II-treated  α -1.S11A/S18A cells
    Figure Legend Snippet: Elution from digoxin-affinity columns of plasma membrane protein containing Na + /K + -ATPase Populations #1 (fractions 1–9), #2 (fractions 10–18) and #3 (fractions 19–22) from control and Ang II-treated α -1.S11A/S18A cells

    Techniques Used:

    Related Articles

    Western Blot:

    Article Title: Laminin-β1 Impairs Spatial Learning through Inhibition of ERK/MAPK and SGK1 Signaling
    Article Snippet: .. The proteins resolved by SDS-PAGE were transferred to a PVDF membrane (Millipore, Bedford, MA) and western blot was performed by using the following antibodies: rabbit anti-LB1, anti-pS422 SGK1 (Santa Cruz Biotechnology, Santa Cruz, CA), anti-laminin- β 2 (LB2), anti-Akt, anti-pS473 Akt (Cell Signaling, MA), anti-SGK1, anti-ERK1/2, anti-pERK1/2 (Millipore), and anti-actin (Chemicon, Temecula, CA). .. The secondary antibody used was an HRP-conjugated goat-anti rabbit IgG antibody (Chemicon).

    Article Title: Nedd4-2 Modulates Renal Na+-Cl− Cotransporter via the Aldosterone-SGK1-Nedd4-2 Pathway
    Article Snippet: .. Anti-Nedd4-2 (Abcam, Cambridge, UK), anti-SGK1 (Sigma), and anti-actin (Sigma) were diluted 1:500, 1:1000, and 1:1000 for immunoblots, respectively. .. Secondary antibodies coupled with horseradish peroxidase (GE Healthcare) were used diluted 1:25,000, and Western blots were revealed with ECL reagents (GE Healthcare or Pierce, Rockford, IL).

    Incubation:

    Article Title: Decreased SGK1 Expression and Function Contributes to Behavioral Deficits Induced by Traumatic StressA Molecular Clue to PTSD
    Article Snippet: .. The membranes were incubated in 5% dry, nonfat milk blocking solution (Tris-buffered saline [TBS], 0.1% Tween 20) for 1 h and then incubated at 4°C overnight with primary anti-SGK1 (1:400, Novagen), anti-PSD-95 (1:1000, Invitrogen), anti-GluR1 (1:1000, Abcam), anti-GR (1:1000, Santa Cruz), and anti-GAPDH (1:10000, Advanced Immunochemical) in 1% dry nonfat milk TBST. .. After 3 x 15 min washes membranes were incubated for 1 h with secondary, horseradish peroxidase (HRP) conjugated antibodies (1:10000, Vector Laboratories, Burlingame, California).

    Article Title: Epithelial Sodium Channel Regulation by Cell Surface-associated Serum- and Glucocorticoid-regulated Kinase 1 *
    Article Snippet: .. Sections were incubated with blocking solution (PBS, 3% BSA, 10% donkey serum) for 40 min and then incubated with rabbit anti-sgk1 antibody (1:100, Sigma) and goat anti-aquaporin-2 (1:50, Santa Cruz Biotechnology) antibodies at 4 °C overnight. .. After washing with PBS, sections were incubated with AlexaFluor 546-conjugated donkey anti-rabbit IgG (1:800, Invitrogen) and AlexaFluor 633-conjugated donkey anti-goat IgG (1:800, Invitrogen).

    Article Title: Epithelial Sodium Channel Regulation by Cell Surface-associated Serum- and Glucocorticoid-regulated Kinase 1 *
    Article Snippet: .. Aldosterone-stimulated mpkCCDc14 cells grown on permeable supports were washed twice with ice-cold PBS, fixed in 4% paraformaldehyde in PBS, rinsed, and incubated in blocking solution (PBS, 3% BSA, 10% donkey serum) for 1 h. Cells were then incubated with anti-sgk1 antibody (1:100, Sigma) or normal rabbit IgG (at a concentration of IgG equivalent to the anti-sgk1 antibody concentration) at 4 °C overnight. .. After washing with PBS, cells were incubated in AlexaFluor 488-conjugated donkey anti-rabbit IgG (1:800, Invitrogen).

    Article Title: A Role for Transcriptional Repressor Methyl-CpG-Binding Protein 2 and Plasticity-Related Gene Serum- and Glucocorticoid-Inducible Kinase 1 in the Induction of Inflammatory Pain States
    Article Snippet: .. Membranes were blocked in 10 m m Tris-HCl, pH 7.5, 150 m m NaCl, 0.05% Tween 20 (Sigma), and 0.24% I-Block (Tropix, Bedford, MA) and incubated with SGK1 antibody (1:1000; overnight at 4°C), FKBP (1:200; overnight at 4°C), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:2000; 2 h room temperature; Chemicon, Chandlers Ford, UK). .. An appropriate HRP-conjugated secondary antibody was incubated for 45 min (1:2000).

    SDS Page:

    Article Title: Laminin-β1 Impairs Spatial Learning through Inhibition of ERK/MAPK and SGK1 Signaling
    Article Snippet: .. The proteins resolved by SDS-PAGE were transferred to a PVDF membrane (Millipore, Bedford, MA) and western blot was performed by using the following antibodies: rabbit anti-LB1, anti-pS422 SGK1 (Santa Cruz Biotechnology, Santa Cruz, CA), anti-laminin- β 2 (LB2), anti-Akt, anti-pS473 Akt (Cell Signaling, MA), anti-SGK1, anti-ERK1/2, anti-pERK1/2 (Millipore), and anti-actin (Chemicon, Temecula, CA). .. The secondary antibody used was an HRP-conjugated goat-anti rabbit IgG antibody (Chemicon).

    Blocking Assay:

    Article Title: Decreased SGK1 Expression and Function Contributes to Behavioral Deficits Induced by Traumatic StressA Molecular Clue to PTSD
    Article Snippet: .. The membranes were incubated in 5% dry, nonfat milk blocking solution (Tris-buffered saline [TBS], 0.1% Tween 20) for 1 h and then incubated at 4°C overnight with primary anti-SGK1 (1:400, Novagen), anti-PSD-95 (1:1000, Invitrogen), anti-GluR1 (1:1000, Abcam), anti-GR (1:1000, Santa Cruz), and anti-GAPDH (1:10000, Advanced Immunochemical) in 1% dry nonfat milk TBST. .. After 3 x 15 min washes membranes were incubated for 1 h with secondary, horseradish peroxidase (HRP) conjugated antibodies (1:10000, Vector Laboratories, Burlingame, California).

    Article Title: Epithelial Sodium Channel Regulation by Cell Surface-associated Serum- and Glucocorticoid-regulated Kinase 1 *
    Article Snippet: .. Sections were incubated with blocking solution (PBS, 3% BSA, 10% donkey serum) for 40 min and then incubated with rabbit anti-sgk1 antibody (1:100, Sigma) and goat anti-aquaporin-2 (1:50, Santa Cruz Biotechnology) antibodies at 4 °C overnight. .. After washing with PBS, sections were incubated with AlexaFluor 546-conjugated donkey anti-rabbit IgG (1:800, Invitrogen) and AlexaFluor 633-conjugated donkey anti-goat IgG (1:800, Invitrogen).

    Article Title: Epithelial Sodium Channel Regulation by Cell Surface-associated Serum- and Glucocorticoid-regulated Kinase 1 *
    Article Snippet: .. Aldosterone-stimulated mpkCCDc14 cells grown on permeable supports were washed twice with ice-cold PBS, fixed in 4% paraformaldehyde in PBS, rinsed, and incubated in blocking solution (PBS, 3% BSA, 10% donkey serum) for 1 h. Cells were then incubated with anti-sgk1 antibody (1:100, Sigma) or normal rabbit IgG (at a concentration of IgG equivalent to the anti-sgk1 antibody concentration) at 4 °C overnight. .. After washing with PBS, cells were incubated in AlexaFluor 488-conjugated donkey anti-rabbit IgG (1:800, Invitrogen).

    Concentration Assay:

    Article Title: Epithelial Sodium Channel Regulation by Cell Surface-associated Serum- and Glucocorticoid-regulated Kinase 1 *
    Article Snippet: .. Aldosterone-stimulated mpkCCDc14 cells grown on permeable supports were washed twice with ice-cold PBS, fixed in 4% paraformaldehyde in PBS, rinsed, and incubated in blocking solution (PBS, 3% BSA, 10% donkey serum) for 1 h. Cells were then incubated with anti-sgk1 antibody (1:100, Sigma) or normal rabbit IgG (at a concentration of IgG equivalent to the anti-sgk1 antibody concentration) at 4 °C overnight. .. After washing with PBS, cells were incubated in AlexaFluor 488-conjugated donkey anti-rabbit IgG (1:800, Invitrogen).

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    Millipore anti na k atpase a1
    Anti Na K Atpase A1, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti na k atpase a1/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti na k atpase a1 - by Bioz Stars, 2020-09
    97/100 stars
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