ap conjugated sheep anti digoxigenin fab fragments  (Roche)

 
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    Structured Review

    Roche ap conjugated sheep anti digoxigenin fab fragments
    A) Effects of acute ethanol on MCH cell density as measured by <t>digoxigenin-labeled</t> ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p
    Ap Conjugated Sheep Anti Digoxigenin Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 83 stars, based on 65 article reviews
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    83/100 stars

    Images

    1) Product Images from "Role of melanin-concentrating hormone in the control of ethanol consumption: Region-specific effects revealed by expression and injection studies"

    Article Title: Role of melanin-concentrating hormone in the control of ethanol consumption: Region-specific effects revealed by expression and injection studies

    Journal: Physiology & behavior

    doi: 10.1016/j.physbeh.2010.07.009

    A) Effects of acute ethanol on MCH cell density as measured by digoxigenin-labeled ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p
    Figure Legend Snippet: A) Effects of acute ethanol on MCH cell density as measured by digoxigenin-labeled ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p

    Techniques Used: Labeling, In Situ Hybridization, Expressing

    2) Product Images from "PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION"

    Article Title: PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION

    Journal: Neuroscience

    doi: 10.1016/j.neuroscience.2012.05.066

    Prenatal ethanol increases the density of peptide-expressing neurons in P0 offspring prenatally exposed to 1 g/kg or 3 g/kg ethanol relative to control ( n = 6–8/group), as assessed by in situ hybridization histochemistry with digoxigenin-labeled
    Figure Legend Snippet: Prenatal ethanol increases the density of peptide-expressing neurons in P0 offspring prenatally exposed to 1 g/kg or 3 g/kg ethanol relative to control ( n = 6–8/group), as assessed by in situ hybridization histochemistry with digoxigenin-labeled

    Techniques Used: Expressing, In Situ Hybridization, Labeling

    3) Product Images from "Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon "

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon

    Journal: Infection and Immunity

    doi: 10.1128/IAI.71.2.985-990.2003

    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with DIG-Stx1B plus HRP-anti-DIG antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Figure Legend Snippet: Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with DIG-Stx1B plus HRP-anti-DIG antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.

    Techniques Used: Thin Layer Chromatography, Staining, Purification

    4) Product Images from "Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution"

    Article Title: Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution

    Journal: Virology Journal

    doi: 10.1186/1743-422X-7-38

    Comparison of binding with digoxigenin-conjugated MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.
    Figure Legend Snippet: Comparison of binding with digoxigenin-conjugated MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.

    Techniques Used: Binding Assay

    Influence of the conjugation method of MAL-I and -II lectins on the staining intensities in duck small intestines . Biotinylated MAL-I (A) and MAL-II (a) both resulted in epithelial cell binding (black arrows), but MAL-II (a) was additionally staining the goblet cells (red arrow). For both lectins binding was abolished by sialidase treatment of the sections (B, b). Digoxigenin labelled MAL-I (C) and MAL-II (c) failed to bind to the same tissues.
    Figure Legend Snippet: Influence of the conjugation method of MAL-I and -II lectins on the staining intensities in duck small intestines . Biotinylated MAL-I (A) and MAL-II (a) both resulted in epithelial cell binding (black arrows), but MAL-II (a) was additionally staining the goblet cells (red arrow). For both lectins binding was abolished by sialidase treatment of the sections (B, b). Digoxigenin labelled MAL-I (C) and MAL-II (c) failed to bind to the same tissues.

    Techniques Used: Conjugation Assay, Staining, Binding Assay

    5) Product Images from "Increased orexin and melanin-concentrating hormone expression in the perifornical lateral hypothalamus of rats prone to overconsuming a fat-rich diet"

    Article Title: Increased orexin and melanin-concentrating hormone expression in the perifornical lateral hypothalamus of rats prone to overconsuming a fat-rich diet

    Journal: Pharmacology, biochemistry, and behavior

    doi: 10.1016/j.pbb.2010.06.013

    Expression of OX and MCH mRNA in the PF vs LH regions of the HFC and control rats following 5 days of high-fat diet consumption, as measured via digoxigenin-labeled ISH (Experiment 2). (a) The data (mean ± SEM), expressed as cell density, show a significant increase in expression of OX in the PF, but not LH region, and of MCH in both PF and LH regions of HFC compared to control animals (* p
    Figure Legend Snippet: Expression of OX and MCH mRNA in the PF vs LH regions of the HFC and control rats following 5 days of high-fat diet consumption, as measured via digoxigenin-labeled ISH (Experiment 2). (a) The data (mean ± SEM), expressed as cell density, show a significant increase in expression of OX in the PF, but not LH region, and of MCH in both PF and LH regions of HFC compared to control animals (* p

    Techniques Used: Expressing, Labeling, In Situ Hybridization

    6) Product Images from "Glutamatergic input to the lateral hypothalamus stimulates ethanol intake: Role of orexin and melanin-concentrating hormone"

    Article Title: Glutamatergic input to the lateral hypothalamus stimulates ethanol intake: Role of orexin and melanin-concentrating hormone

    Journal: Alcoholism, clinical and experimental research

    doi: 10.1111/j.1530-0277.2012.01854.x

    (A) The stimulatory effect of glutamate on OX expression was confirmed using in situ hybridization with a digoxigenin-labeled probe (n = 5–6/group). In the LH, NMDA significantly increased the expression of OX in both PF and LH region, while AMPA
    Figure Legend Snippet: (A) The stimulatory effect of glutamate on OX expression was confirmed using in situ hybridization with a digoxigenin-labeled probe (n = 5–6/group). In the LH, NMDA significantly increased the expression of OX in both PF and LH region, while AMPA

    Techniques Used: Expressing, In Situ Hybridization, Labeling

    7) Product Images from "Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain"

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain

    Journal: Neural Development

    doi: 10.1186/1749-8104-6-10

    Inactivation of antibody-POD conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and digoxigenin-labeled nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Figure Legend Snippet: Inactivation of antibody-POD conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and digoxigenin-labeled nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.

    Techniques Used: Labeling, Expressing, Fluorescence In Situ Hybridization, Incubation, Microscopy

    Positive effects of substituted phenol compounds on the TSA-POD reaction . Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for nkx6.1 are shown with anterior to the left. (A) Control (CTL); (B, C) vanillin added; (D, E) 4-iodophenol added. In all embryos shown, dextran sulfate was included in the hybridization and TSA-POD reaction. Above each panel signal-to-noise ratios (s/n) and compound concentrations are indicated. Black and white pictures were recorded with identical exposure times. Images were false-colored with help of the ImageJ software and no further adjustments or other image processing were performed. Scale bar = 100 μm.
    Figure Legend Snippet: Positive effects of substituted phenol compounds on the TSA-POD reaction . Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for nkx6.1 are shown with anterior to the left. (A) Control (CTL); (B, C) vanillin added; (D, E) 4-iodophenol added. In all embryos shown, dextran sulfate was included in the hybridization and TSA-POD reaction. Above each panel signal-to-noise ratios (s/n) and compound concentrations are indicated. Black and white pictures were recorded with identical exposure times. Images were false-colored with help of the ImageJ software and no further adjustments or other image processing were performed. Scale bar = 100 μm.

    Techniques Used: Labeling, CTL Assay, Hybridization, Software

    Bench-made fluorescent tyramides applied in zebrafish whole-mount FISH . Embryos were viewed on an Axioplan II microscope (Carl Zeiss). For excitation, a mercury burner (HBO 103 OSRAM) was used. Lateral views of zebrafish brains are shown with anterior to the left. Scale bar = 100 μm. ( A-C ) Zebrafish embryos at 28 hpf hybridized to a digoxigenin-labeled nkx6.1 antisense RNA probe. Images were captured with an Orca digital camera (Hamamatsu). As POD substrates, three different bench-made fluorogenic tyramides at a 1:250 dilution were used: (A) FAM-tyramide (exposure time, 100 ms; Chroma-filter 41001); (B) TAMRA-tyramide (exposure time, 4 ms; Chroma-filter 41002b); and (C) DyLight633-tyramide (exposure time, 120 ms; Chroma-filter 41008). ( D-F ) Zebrafish embryos at 24 hpf hybridized to a digoxigenin-labeled shha antisense RNA probe. Images were recorded with an Axiocam digital color camera (Carl Zeiss) using identical exposure times. Transcript distribution was visualized with (D) fluorescein-tyramide from Perkin Elmer (SAT701B001EA) at a 1:100 dilution, (E) bench-made FAM-tyramide at a 1:250 dilution, or (F) a 1:100 dilution.
    Figure Legend Snippet: Bench-made fluorescent tyramides applied in zebrafish whole-mount FISH . Embryos were viewed on an Axioplan II microscope (Carl Zeiss). For excitation, a mercury burner (HBO 103 OSRAM) was used. Lateral views of zebrafish brains are shown with anterior to the left. Scale bar = 100 μm. ( A-C ) Zebrafish embryos at 28 hpf hybridized to a digoxigenin-labeled nkx6.1 antisense RNA probe. Images were captured with an Orca digital camera (Hamamatsu). As POD substrates, three different bench-made fluorogenic tyramides at a 1:250 dilution were used: (A) FAM-tyramide (exposure time, 100 ms; Chroma-filter 41001); (B) TAMRA-tyramide (exposure time, 4 ms; Chroma-filter 41002b); and (C) DyLight633-tyramide (exposure time, 120 ms; Chroma-filter 41008). ( D-F ) Zebrafish embryos at 24 hpf hybridized to a digoxigenin-labeled shha antisense RNA probe. Images were recorded with an Axiocam digital color camera (Carl Zeiss) using identical exposure times. Transcript distribution was visualized with (D) fluorescein-tyramide from Perkin Elmer (SAT701B001EA) at a 1:100 dilution, (E) bench-made FAM-tyramide at a 1:250 dilution, or (F) a 1:100 dilution.

    Techniques Used: Fluorescence In Situ Hybridization, Microscopy, Labeling, Mass Spectrometry

    Optimization of hybridization and POD reaction by dextran sulfate . (A, C, E, G) Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for dbx1a are shown with anterior to the left. Above each panel signal-to-noise ratios (s/n) are indicated and whether dextran sulfate was added (+) or not (-) to the hybridization buffer (Hyb) and/or TSA reaction. (B, D, F, H) Embryos hybridized without probe were used as negative controls to assess background noise. Black-and-white pictures were recorded with identical exposure times using an Orca digital camera (Hamamatsu) on an Axioplan II microscope (Carl Zeiss). Images were false-colored with help of the ImageJ software and no further adjustments or other image processing was performed. Scale bar = 100 μm.
    Figure Legend Snippet: Optimization of hybridization and POD reaction by dextran sulfate . (A, C, E, G) Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for dbx1a are shown with anterior to the left. Above each panel signal-to-noise ratios (s/n) are indicated and whether dextran sulfate was added (+) or not (-) to the hybridization buffer (Hyb) and/or TSA reaction. (B, D, F, H) Embryos hybridized without probe were used as negative controls to assess background noise. Black-and-white pictures were recorded with identical exposure times using an Orca digital camera (Hamamatsu) on an Axioplan II microscope (Carl Zeiss). Images were false-colored with help of the ImageJ software and no further adjustments or other image processing was performed. Scale bar = 100 μm.

    Techniques Used: Hybridization, Labeling, Microscopy, Software

    8) Product Images from "Prenatal Exposure to Nicotine Stimulates Neurogenesis of Orexigenic Peptide-Expressing Neurons in Hypothalamus and Amygdala"

    Article Title: Prenatal Exposure to Nicotine Stimulates Neurogenesis of Orexigenic Peptide-Expressing Neurons in Hypothalamus and Amygdala

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.5835-12.2013

    Prenatal nicotine increases the density of peptide-expressing neurons in P15 offspring exposed to 1.5 mg/kg/d nicotine relative to control ( n = 4–8/group), as assessed by ISH with digoxigenin-labeled probes. A , Density of peptide-expressing neurons. Data are mean ± SEM, * p
    Figure Legend Snippet: Prenatal nicotine increases the density of peptide-expressing neurons in P15 offspring exposed to 1.5 mg/kg/d nicotine relative to control ( n = 4–8/group), as assessed by ISH with digoxigenin-labeled probes. A , Density of peptide-expressing neurons. Data are mean ± SEM, * p

    Techniques Used: Expressing, In Situ Hybridization, Labeling

    9) Product Images from "Differential effects of acute and chronic ethanol exposure on orexin expression in the perifornical lateral hypothalamus"

    Article Title: Differential effects of acute and chronic ethanol exposure on orexin expression in the perifornical lateral hypothalamus

    Journal: Alcoholism, clinical and experimental research

    doi: 10.1111/j.1530-0277.2010.01161.x

    Effects of acute ethanol gavage on OX mRNA and peptide expression in the LH vs PF regions, as measured via (a) digoxigenin-labeled ISH and (b) immunofluorescence histochemistry (Experiment 4). The data (mean ±SEM) for the 3 groups (n=5/group),
    Figure Legend Snippet: Effects of acute ethanol gavage on OX mRNA and peptide expression in the LH vs PF regions, as measured via (a) digoxigenin-labeled ISH and (b) immunofluorescence histochemistry (Experiment 4). The data (mean ±SEM) for the 3 groups (n=5/group),

    Techniques Used: Expressing, Labeling, In Situ Hybridization, Immunofluorescence

    Photomicrographs illustrating the stimulatory effect of 0.75 g/kg ethanol on OX (a) mRNA expression and 2.5 g/kg ethanol on (b) peptide levels in the LH, as measured using digoxigenin-labeled ISH and immunofluorescence histochemistry as graphed in
    Figure Legend Snippet: Photomicrographs illustrating the stimulatory effect of 0.75 g/kg ethanol on OX (a) mRNA expression and 2.5 g/kg ethanol on (b) peptide levels in the LH, as measured using digoxigenin-labeled ISH and immunofluorescence histochemistry as graphed in

    Techniques Used: Expressing, Labeling, In Situ Hybridization, Immunofluorescence

    Related Articles

    Clone Assay:

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    Article Snippet: Sense and antisense LIF and LIFR riboprobes were generated using partial cDNAs cloned into pCRII vectors by linearizing with appropriate restriction enzymes and labeling with digoxigenin (DIG)-UTP using a DIG RNA Labeling kit (Roche, Indianapolis, IN, USA). .. Probes bound to the section were detected immunologically using sheep anti-DIG Fab fragments covalently coupled to alkaline phosphatase and nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) as chromogenic substrate, according to the manufacturer’s protocol (Roche, USA).

    Mass Spectrometry:

    Article Title: Inhibition of H3K27me3 Histone Demethylase Activity Prevents the Proliferative Regeneration of Zebrafish Lateral Line Neuromasts
    Article Snippet: Briefly, the embryos were depigmented with 1-phenyl-2-thiourea (Sigma-Aldrich), euthanized in MS-222, and fixed overnight with 4% PFA at 4°C. .. After washing for 15 min with 75, 50, and 25% hybridization buffer and 2 × SSCT (20 × SSC, Life technologies; 0.1% Tween-20) and for 30 min twice in 0.2 × SSC at 65°C, the embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche) at a 1:4,000 dilution in blocking buffer overnight at 4°C.

    Article Title: Effect of JNK inhibitor SP600125 on hair cell regeneration in zebrafish (Danio rerio) larvae
    Article Snippet: Briefly, the embryos were depigmented with 1-phenyl-2-thiourea (PTU, Sigma-Aldrich, cat. no. P7629), euthanized in MS-222, and fixed overnight with 4% PFA at 4°C. .. After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C.

    Blocking Assay:

    Article Title: Role of miR‐155 in the regulation of MMP‐16 expression in intervertebral disc degeneration
    Article Snippet: .. The slides were then washed in 0.2× SSC for 5 min at 57°C and then incubated in blocking buffer for 1 h at room temperature, and then in sheep Anti‐DIG Fab fragments, POD conjugated (Roche, Strasbourg, France) at room temperature overnight. .. Subsequently, the sections were managed with TSA(TM) Biotin System (PerkinElmer) for 30 min followed by cy3‐avidin (CST) for 2 h. 4′, 6‐Diamidino‐2‐phenylindole (DAPI) staining was used as the final step in the fluorescent staining procedure to label the cell nuclei.

    Article Title: The claustrum supports resilience to distraction
    Article Snippet: .. Slides were mounted with wash buffer, followed by blocking buffer and immunolabeled with sheep Anti-Digoxigenin-AP Fab fragments (Roche; catalog No. 11093274910; final dilution to 1:5000 in blocking buffer). .. Slides were then washed with wash buffer and mounted with a chromogenic reaction eliciting substrate (BCIP+NBT ready mix, Roche), covered with coverslips to prevent contact with air and incubated in the dark, overnight at room temperature.

    Article Title: Inhibition of H3K27me3 Histone Demethylase Activity Prevents the Proliferative Regeneration of Zebrafish Lateral Line Neuromasts
    Article Snippet: .. After washing for 15 min with 75, 50, and 25% hybridization buffer and 2 × SSCT (20 × SSC, Life technologies; 0.1% Tween-20) and for 30 min twice in 0.2 × SSC at 65°C, the embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche) at a 1:4,000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

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    Article Snippet: .. After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Production of Hybrid-IgG/IgA Plantibodies with Neutralizing Activity against Shiga Toxin 1
    Article Snippet: Paragraph title: ELISA ... Binding of digoxigenin-conjugated Stx1B (25 ng/ml; DIG-Stx1B), which had been pretreated with or without the plantibody for 1 h, to the immobilized Gb3 was determined with HRP-sheep anti-digoxigenin Fab fragments (1∶500 dilution; Roche Diagnostics, Basel, Switzerland).

    Incubation:

    Article Title: Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution
    Article Snippet: .. Subsequently, the slides were incubated with 1:200 sheep alkaline phosphatase conjugated anti-digoxigenin Fab fragments (Roche) and developed with New Fuchsin (Dako). .. -Expression of α2-3 linked Sia- Two different isoforms of the Maackia amurensis agglutinin (MAA), MAL-I and MAL-II that both bind to the α2-3-linked Sia moiety of the receptor, were used.

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain
    Article Snippet: Embryos were blocked in 8% normal sheep serum (Sigma S2263) in PBST for 1 h at room temperature with gentle agitation and then incubated with the appropriate antibody overnight without agitation at 4°C. .. Sheep-anti-digoxigenin-POD Fab fragments (Roche 11207733910) were diluted 1:500, anti-dinitrophenyl-POD (PerkinElmer TSA Plus DNP System NEL747A001KT) was diluted 1:100 and the rabbit-anti-fluorescein/Oregon Green 488-POD (Molecular Probes: Eugene, OR, USA A21253) was diluted 1:500.

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    Article Snippet: On the following day, for Egr2 DIG-labeled probe hybridization, pre-hybridization buffer was replaced with hybridization buffer containing 200 ng Egr2 DIG-labeled RNA probe, slides were covered with hybridization cover (Bio-Labs HybriSlip™), placed in a humidified chamber and incubated at 71.5°C overnight. .. Slides were mounted with wash buffer, followed by blocking buffer and immunolabeled with sheep Anti-Digoxigenin-AP Fab fragments (Roche; catalog No. 11093274910; final dilution to 1:5000 in blocking buffer).

    Article Title: Production of Hybrid-IgG/IgA Plantibodies with Neutralizing Activity against Shiga Toxin 1
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    Article Title: Inhibition of H3K27me3 Histone Demethylase Activity Prevents the Proliferative Regeneration of Zebrafish Lateral Line Neuromasts
    Article Snippet: .. After washing for 15 min with 75, 50, and 25% hybridization buffer and 2 × SSCT (20 × SSC, Life technologies; 0.1% Tween-20) and for 30 min twice in 0.2 × SSC at 65°C, the embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche) at a 1:4,000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

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    Article Snippet: .. After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

    Expressing:

    Article Title: Differential effects of acute and chronic ethanol exposure on orexin expression in the perifornical lateral hypothalamus
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal. .. Gene expression level was measured by semi-quantification with Image-Pro Plus software, version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) and was expressed as cells /mm2, reflecting density of mRNA containing cells.

    Article Title: Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution
    Article Snippet: Paragraph title: -Expression of α2-6 linked Sia- ... Subsequently, the slides were incubated with 1:200 sheep alkaline phosphatase conjugated anti-digoxigenin Fab fragments (Roche) and developed with New Fuchsin (Dako).

    Article Title: Glutamatergic input to the lateral hypothalamus stimulates ethanol intake: Role of orexin and melanin-concentrating hormone
    Article Snippet: In Experiment 3, the mRNA expression of OX and MCH was also measured with digoxigenin (DIG)-labeled antisense RNA probes and 30-μm free-floating cryostat sections were used for ISH histochemistry as described previously ( ). .. AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT/BCIP (Roche, Nutley, NJ) were used to visualize the signal.

    Article Title: Prenatal Exposure to Nicotine Stimulates Neurogenesis of Orexigenic Peptide-Expressing Neurons in Hypothalamus and Amygdala
    Article Snippet: In P15 offspring of Group 2, ISH with a digoxigenin-labeled probe that provides better visualization was performed to more clearly demonstrate the anatomical distribution of changes in peptide expression in the control and 1.5 mg/kg/d nicotine groups as described previously ( ; ). .. AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal.

    Article Title: Role of melanin-concentrating hormone in the control of ethanol consumption: Region-specific effects revealed by expression and injection studies
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal. .. Gene expression level was measured by semi-quantification with Image-Pro Plus software, version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) and was expressed as cells /mm2 , reflecting density of mRNA containing cells.

    Article Title: PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION
    Article Snippet: For better visualization, in situ hydridization histochemistry with a digoxigenin-labeled probe (digoxigenin-labeled in situ hybridization; DIG-ISH) was performed to reveal the anatomical distribution at birth (P0) of changes in peptide expression in the control, 1 g/kg ethanol and 3 g/kg dose ethanol groups ( n = 6–8/group) as described ( , ). .. AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics, Indianapolis, IN, USA) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal.

    Article Title: Increased orexin and melanin-concentrating hormone expression in the perifornical lateral hypothalamus of rats prone to overconsuming a fat-rich diet
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal. .. Gene expression level was measured by semi-quantification with Image-Pro Plus software, version 4.5 (Media Cybernetics, Inc., Silver Spring, MD) and was expressed as cells/mm2 , reflecting density of mRNA containing cells.

    Hybridization:

    Article Title: Role of miR‐155 in the regulation of MMP‐16 expression in intervertebral disc degeneration
    Article Snippet: In Situ Hybridization (ISH) To detect and localize miR‐155, we exploited the higher specificity and hybridization efficiency of locked nucleic acid (LNA) probes in frozen human NP tissues., LNA hybridization probes labeled with digoxigenin (DIG) complementary to human mature miR‐155 and negative controls (scramble probes) were purchased from Exiqon (Copenhagen, Denmark). .. The slides were then washed in 0.2× SSC for 5 min at 57°C and then incubated in blocking buffer for 1 h at room temperature, and then in sheep Anti‐DIG Fab fragments, POD conjugated (Roche, Strasbourg, France) at room temperature overnight.

    Article Title: The claustrum supports resilience to distraction
    Article Snippet: On the following day, for Egr2 DIG-labeled probe hybridization, pre-hybridization buffer was replaced with hybridization buffer containing 200 ng Egr2 DIG-labeled RNA probe, slides were covered with hybridization cover (Bio-Labs HybriSlip™), placed in a humidified chamber and incubated at 71.5°C overnight. .. Slides were mounted with wash buffer, followed by blocking buffer and immunolabeled with sheep Anti-Digoxigenin-AP Fab fragments (Roche; catalog No. 11093274910; final dilution to 1:5000 in blocking buffer).

    Article Title: Inhibition of H3K27me3 Histone Demethylase Activity Prevents the Proliferative Regeneration of Zebrafish Lateral Line Neuromasts
    Article Snippet: .. After washing for 15 min with 75, 50, and 25% hybridization buffer and 2 × SSCT (20 × SSC, Life technologies; 0.1% Tween-20) and for 30 min twice in 0.2 × SSC at 65°C, the embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche) at a 1:4,000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

    Article Title: Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy
    Article Snippet: After hybridization, sections were washed for 30 min in 2× SSC at room temperature, 1 h in 2× SSC at 65°C, and 1 h in 0.1× SSC at 65°C. .. Probes bound to the section were detected immunologically using sheep anti-DIG Fab fragments covalently coupled to alkaline phosphatase and nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) as chromogenic substrate, according to the manufacturer’s protocol (Roche, USA).

    Article Title: Effect of JNK inhibitor SP600125 on hair cell regeneration in zebrafish (Danio rerio) larvae
    Article Snippet: .. After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

    Immunohistochemistry:

    Article Title: Role of miR‐155 in the regulation of MMP‐16 expression in intervertebral disc degeneration
    Article Snippet: The slides were then washed in 0.2× SSC for 5 min at 57°C and then incubated in blocking buffer for 1 h at room temperature, and then in sheep Anti‐DIG Fab fragments, POD conjugated (Roche, Strasbourg, France) at room temperature overnight. .. The semi‐quantitative analysis method of the ISH results and immunohistochemical analysis method is the same.

    Chromatography:

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon
    Article Snippet: Recombinant binding subunits of Stx1 (Stx1B) were expressed in E. coli JM105(pVT1-B5) cells, and proteins were purified to homogeneity by anion-exchange chromatography and chromatofocusing as described previously ( ). .. Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Immunolabeling:

    Article Title: The claustrum supports resilience to distraction
    Article Snippet: .. Slides were mounted with wash buffer, followed by blocking buffer and immunolabeled with sheep Anti-Digoxigenin-AP Fab fragments (Roche; catalog No. 11093274910; final dilution to 1:5000 in blocking buffer). .. Slides were then washed with wash buffer and mounted with a chromogenic reaction eliciting substrate (BCIP+NBT ready mix, Roche), covered with coverslips to prevent contact with air and incubated in the dark, overnight at room temperature.

    Generated:

    Article Title: Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy
    Article Snippet: Sense and antisense LIF and LIFR riboprobes were generated using partial cDNAs cloned into pCRII vectors by linearizing with appropriate restriction enzymes and labeling with digoxigenin (DIG)-UTP using a DIG RNA Labeling kit (Roche, Indianapolis, IN, USA). .. Probes bound to the section were detected immunologically using sheep anti-DIG Fab fragments covalently coupled to alkaline phosphatase and nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) as chromogenic substrate, according to the manufacturer’s protocol (Roche, USA).

    Recombinant:

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon
    Article Snippet: Recombinant binding subunits of Stx1 (Stx1B) were expressed in E. coli JM105(pVT1-B5) cells, and proteins were purified to homogeneity by anion-exchange chromatography and chromatofocusing as described previously ( ). .. Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Article Title: Production of Hybrid-IgG/IgA Plantibodies with Neutralizing Activity against Shiga Toxin 1
    Article Snippet: Binding of digoxigenin-conjugated Stx1B (25 ng/ml; DIG-Stx1B), which had been pretreated with or without the plantibody for 1 h, to the immobilized Gb3 was determined with HRP-sheep anti-digoxigenin Fab fragments (1∶500 dilution; Roche Diagnostics, Basel, Switzerland). .. Recombinant purified Stx1B and DIG-Stx1B were prepared as described previously .

    Microscopy:

    Article Title: Inhibition of H3K27me3 Histone Demethylase Activity Prevents the Proliferative Regeneration of Zebrafish Lateral Line Neuromasts
    Article Snippet: After washing for 15 min with 75, 50, and 25% hybridization buffer and 2 × SSCT (20 × SSC, Life technologies; 0.1% Tween-20) and for 30 min twice in 0.2 × SSC at 65°C, the embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche) at a 1:4,000 dilution in blocking buffer overnight at 4°C. .. Finally, the color reaction was stopped by adding PBST, and the embryos were observed under a bright field microscope (Nikon Instruments).

    Article Title: Prenatal Exposure to Nicotine Stimulates Neurogenesis of Orexigenic Peptide-Expressing Neurons in Hypothalamus and Amygdala
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal. .. Sections were viewed on a Leitz microscope (10× objective), and the images were captured with a Nikon DXM 1200 digital camera and analyzed using Image-Pro Plus software (Version 4.5; Media Cybernetics), as described below.

    Article Title: Effect of JNK inhibitor SP600125 on hair cell regeneration in zebrafish (Danio rerio) larvae
    Article Snippet: After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C. .. Finally, the color reaction was stopped by adding PBST, and the embryos were observed under a bright field microscope (Nikon Instruments).

    Article Title: PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics, Indianapolis, IN, USA) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal. .. Sections were viewed on a Leitz microscope (10× objective).

    Purification:

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon
    Article Snippet: The purified Stx1B subunits were labeled with digoxigenin (DIG-Stx1B) as described previously ( ). .. Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Article Title: Production of Hybrid-IgG/IgA Plantibodies with Neutralizing Activity against Shiga Toxin 1
    Article Snippet: Binding of digoxigenin-conjugated Stx1B (25 ng/ml; DIG-Stx1B), which had been pretreated with or without the plantibody for 1 h, to the immobilized Gb3 was determined with HRP-sheep anti-digoxigenin Fab fragments (1∶500 dilution; Roche Diagnostics, Basel, Switzerland). .. Recombinant purified Stx1B and DIG-Stx1B were prepared as described previously .

    Labeling:

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon
    Article Snippet: The purified Stx1B subunits were labeled with digoxigenin (DIG-Stx1B) as described previously ( ). .. Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Article Title: Role of miR‐155 in the regulation of MMP‐16 expression in intervertebral disc degeneration
    Article Snippet: In Situ Hybridization (ISH) To detect and localize miR‐155, we exploited the higher specificity and hybridization efficiency of locked nucleic acid (LNA) probes in frozen human NP tissues., LNA hybridization probes labeled with digoxigenin (DIG) complementary to human mature miR‐155 and negative controls (scramble probes) were purchased from Exiqon (Copenhagen, Denmark). .. The slides were then washed in 0.2× SSC for 5 min at 57°C and then incubated in blocking buffer for 1 h at room temperature, and then in sheep Anti‐DIG Fab fragments, POD conjugated (Roche, Strasbourg, France) at room temperature overnight.

    Article Title: Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy
    Article Snippet: Sense and antisense LIF and LIFR riboprobes were generated using partial cDNAs cloned into pCRII vectors by linearizing with appropriate restriction enzymes and labeling with digoxigenin (DIG)-UTP using a DIG RNA Labeling kit (Roche, Indianapolis, IN, USA). .. Probes bound to the section were detected immunologically using sheep anti-DIG Fab fragments covalently coupled to alkaline phosphatase and nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) as chromogenic substrate, according to the manufacturer’s protocol (Roche, USA).

    Article Title: Effect of JNK inhibitor SP600125 on hair cell regeneration in zebrafish (Danio rerio) larvae
    Article Snippet: For hybridization, the labeled probes were added to the hybridization buffer at 65°C overnight. .. After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C.

    In Situ Hybridization:

    Article Title: Differential effects of acute and chronic ethanol exposure on orexin expression in the perifornical lateral hypothalamus
    Article Snippet: Paragraph title: Digoxigenin-labeled In Situ Hybridization Histochemistry ... AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal.

    Article Title: Role of miR‐155 in the regulation of MMP‐16 expression in intervertebral disc degeneration
    Article Snippet: Paragraph title: In Situ Hybridization (ISH) ... The slides were then washed in 0.2× SSC for 5 min at 57°C and then incubated in blocking buffer for 1 h at room temperature, and then in sheep Anti‐DIG Fab fragments, POD conjugated (Roche, Strasbourg, France) at room temperature overnight.

    Article Title: The claustrum supports resilience to distraction
    Article Snippet: Paragraph title: In Situ Hybridization ... Slides were mounted with wash buffer, followed by blocking buffer and immunolabeled with sheep Anti-Digoxigenin-AP Fab fragments (Roche; catalog No. 11093274910; final dilution to 1:5000 in blocking buffer).

    Article Title: Inhibition of H3K27me3 Histone Demethylase Activity Prevents the Proliferative Regeneration of Zebrafish Lateral Line Neuromasts
    Article Snippet: Paragraph title: Whole-mount in situ hybridization ... After washing for 15 min with 75, 50, and 25% hybridization buffer and 2 × SSCT (20 × SSC, Life technologies; 0.1% Tween-20) and for 30 min twice in 0.2 × SSC at 65°C, the embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche) at a 1:4,000 dilution in blocking buffer overnight at 4°C.

    Article Title: Glutamatergic input to the lateral hypothalamus stimulates ethanol intake: Role of orexin and melanin-concentrating hormone
    Article Snippet: Paragraph title: Digoxigenin-Labeled In Situ Hybridization Histochemistry ... AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT/BCIP (Roche, Nutley, NJ) were used to visualize the signal.

    Article Title: Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy
    Article Snippet: Paragraph title: Non-radioactive in situ hybridization ... Probes bound to the section were detected immunologically using sheep anti-DIG Fab fragments covalently coupled to alkaline phosphatase and nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) as chromogenic substrate, according to the manufacturer’s protocol (Roche, USA).

    Article Title: Prenatal Exposure to Nicotine Stimulates Neurogenesis of Orexigenic Peptide-Expressing Neurons in Hypothalamus and Amygdala
    Article Snippet: Paragraph title: Digoxigenin-labeled ISH to measure peptide neuronal density ... AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal.

    Article Title: Role of melanin-concentrating hormone in the control of ethanol consumption: Region-specific effects revealed by expression and injection studies
    Article Snippet: Paragraph title: 2.6 Digoxigenin-Labeled In Situ Hybridization Histochemistry ... AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal.

    Article Title: Effect of JNK inhibitor SP600125 on hair cell regeneration in zebrafish (Danio rerio) larvae
    Article Snippet: Paragraph title: Whole-mount in situ hybridization ... After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C.

    Article Title: PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION
    Article Snippet: Paragraph title: Digoxigenin-labeled in situ hybridization histochemistry ... AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics, Indianapolis, IN, USA) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal.

    Article Title: Increased orexin and melanin-concentrating hormone expression in the perifornical lateral hypothalamus of rats prone to overconsuming a fat-rich diet
    Article Snippet: Paragraph title: 2.9. Digoxigenin-labeled in situ hybridization histochemistry ... AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal.

    Plasmid Preparation:

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon
    Article Snippet: Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan). .. Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Software:

    Article Title: Differential effects of acute and chronic ethanol exposure on orexin expression in the perifornical lateral hypothalamus
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal. .. Gene expression level was measured by semi-quantification with Image-Pro Plus software, version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) and was expressed as cells /mm2, reflecting density of mRNA containing cells.

    Article Title: Glutamatergic input to the lateral hypothalamus stimulates ethanol intake: Role of orexin and melanin-concentrating hormone
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT/BCIP (Roche, Nutley, NJ) were used to visualize the signal. .. Gene expression was measured by semi-quantification with Image-Pro Plus software, version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) and was expressed as number of cells/mm2 , reflecting regional density of mRNA-containing cells.

    Article Title: Prenatal Exposure to Nicotine Stimulates Neurogenesis of Orexigenic Peptide-Expressing Neurons in Hypothalamus and Amygdala
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal. .. Sections were viewed on a Leitz microscope (10× objective), and the images were captured with a Nikon DXM 1200 digital camera and analyzed using Image-Pro Plus software (Version 4.5; Media Cybernetics), as described below.

    Article Title: Role of melanin-concentrating hormone in the control of ethanol consumption: Region-specific effects revealed by expression and injection studies
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal. .. Gene expression level was measured by semi-quantification with Image-Pro Plus software, version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) and was expressed as cells /mm2 , reflecting density of mRNA containing cells.

    Article Title: PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics, Indianapolis, IN, USA) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal. .. The images were captured with a Nikon DXM 1200 digital camera (Nikon, Tokyo, Japan) and analyzed using Image-Pro Plus software (Version 4.5, Media Cybernetics Inc., Silver Spring, MD, USA) on a gray-value scale from 1 to 255.

    Article Title: Increased orexin and melanin-concentrating hormone expression in the perifornical lateral hypothalamus of rats prone to overconsuming a fat-rich diet
    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal. .. Gene expression level was measured by semi-quantification with Image-Pro Plus software, version 4.5 (Media Cybernetics, Inc., Silver Spring, MD) and was expressed as cells/mm2 , reflecting density of mRNA containing cells.

    Binding Assay:

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon
    Article Snippet: Recombinant binding subunits of Stx1 (Stx1B) were expressed in E. coli JM105(pVT1-B5) cells, and proteins were purified to homogeneity by anion-exchange chromatography and chromatofocusing as described previously ( ). .. Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Article Title: Production of Hybrid-IgG/IgA Plantibodies with Neutralizing Activity against Shiga Toxin 1
    Article Snippet: .. Binding of digoxigenin-conjugated Stx1B (25 ng/ml; DIG-Stx1B), which had been pretreated with or without the plantibody for 1 h, to the immobilized Gb3 was determined with HRP-sheep anti-digoxigenin Fab fragments (1∶500 dilution; Roche Diagnostics, Basel, Switzerland). .. Recombinant purified Stx1B and DIG-Stx1B were prepared as described previously .

    In Situ:

    Article Title: PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION
    Article Snippet: For better visualization, in situ hydridization histochemistry with a digoxigenin-labeled probe (digoxigenin-labeled in situ hybridization; DIG-ISH) was performed to reveal the anatomical distribution at birth (P0) of changes in peptide expression in the control, 1 g/kg ethanol and 3 g/kg dose ethanol groups ( n = 6–8/group) as described ( , ). .. AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics, Indianapolis, IN, USA) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal.

    Evaporation:

    Article Title: Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy
    Article Snippet: Prehybridization and hybridization reactions were performed in a box saturated with a 5× SSC-50% formamide solution to avoid evaporation, and no coverslips were used. .. Probes bound to the section were detected immunologically using sheep anti-DIG Fab fragments covalently coupled to alkaline phosphatase and nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) as chromogenic substrate, according to the manufacturer’s protocol (Roche, USA).

    Staining:

    Article Title: Role of miR‐155 in the regulation of MMP‐16 expression in intervertebral disc degeneration
    Article Snippet: The slides were then washed in 0.2× SSC for 5 min at 57°C and then incubated in blocking buffer for 1 h at room temperature, and then in sheep Anti‐DIG Fab fragments, POD conjugated (Roche, Strasbourg, France) at room temperature overnight. .. Subsequently, the sections were managed with TSA(TM) Biotin System (PerkinElmer) for 30 min followed by cy3‐avidin (CST) for 2 h. 4′, 6‐Diamidino‐2‐phenylindole (DAPI) staining was used as the final step in the fluorescent staining procedure to label the cell nuclei.

    Article Title: Inhibition of H3K27me3 Histone Demethylase Activity Prevents the Proliferative Regeneration of Zebrafish Lateral Line Neuromasts
    Article Snippet: After washing for 15 min with 75, 50, and 25% hybridization buffer and 2 × SSCT (20 × SSC, Life technologies; 0.1% Tween-20) and for 30 min twice in 0.2 × SSC at 65°C, the embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche) at a 1:4,000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

    Article Title: Effect of JNK inhibitor SP600125 on hair cell regeneration in zebrafish (Danio rerio) larvae
    Article Snippet: After washing for 15 min with 75%, 50%, and 25% hybridization buffer and 2× SSCT (20× SSC, Life technologies, AM9770; 0.1% Tween-20) and for 30 min twice in 0.2× SSC at 65°C, embryos were blocked for at least 2 h at 4°C in blocking buffer (Roche cat. no._11096176001) and were incubated with preabsorbed sheep anti-digoxigenin-AP Fab fragments (Roche cat. no. 11093274910) at a 1:4000 dilution in blocking buffer overnight at 4°C. .. The next day, the embryos were washed 4 × 30 min with 2 mg/mL BSA in PBST and 3 × 5 min in staining buffer (100 mM Tris (pH 9.5), 100 mM NaCl, and 0.1% Tween-20).

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    Roche ap conjugated sheep anti digoxigenin fab fragments
    A) Effects of acute ethanol on MCH cell density as measured by <t>digoxigenin-labeled</t> ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p
    Ap Conjugated Sheep Anti Digoxigenin Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ap conjugated sheep anti digoxigenin fab fragments/product/Roche
    Average 83 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    ap conjugated sheep anti digoxigenin fab fragments - by Bioz Stars, 2020-02
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    78
    Roche horseradish peroxidase hrp labeled sheep anti digoxigenin dig fab fragments hrp anti dig
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Horseradish Peroxidase Hrp Labeled Sheep Anti Digoxigenin Dig Fab Fragments Hrp Anti Dig, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp labeled sheep anti digoxigenin dig fab fragments hrp anti dig/product/Roche
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp labeled sheep anti digoxigenin dig fab fragments hrp anti dig - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    77
    Roche sheep alkaline phosphatase conjugated anti digoxigenin fab fragments
    Comparison of binding with <t>digoxigenin-conjugated</t> MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.
    Sheep Alkaline Phosphatase Conjugated Anti Digoxigenin Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep alkaline phosphatase conjugated anti digoxigenin fab fragments/product/Roche
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sheep alkaline phosphatase conjugated anti digoxigenin fab fragments - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    Image Search Results


    A) Effects of acute ethanol on MCH cell density as measured by digoxigenin-labeled ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p

    Journal: Physiology & behavior

    Article Title: Role of melanin-concentrating hormone in the control of ethanol consumption: Region-specific effects revealed by expression and injection studies

    doi: 10.1016/j.physbeh.2010.07.009

    Figure Lengend Snippet: A) Effects of acute ethanol on MCH cell density as measured by digoxigenin-labeled ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p

    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal.

    Techniques: Labeling, In Situ Hybridization, Expressing

    Prenatal ethanol increases the density of peptide-expressing neurons in P0 offspring prenatally exposed to 1 g/kg or 3 g/kg ethanol relative to control ( n = 6–8/group), as assessed by in situ hybridization histochemistry with digoxigenin-labeled

    Journal: Neuroscience

    Article Title: PRENATAL ETHANOL EXPOSURE STIMULATES NEUROGENESIS IN HYPOTHALAMIC AND LIMBIC PEPTIDE SYSTEMS: POSSIBLE MECHANISM FOR OFFSPRING ETHANOL OVERCONSUMPTION

    doi: 10.1016/j.neuroscience.2012.05.066

    Figure Lengend Snippet: Prenatal ethanol increases the density of peptide-expressing neurons in P0 offspring prenatally exposed to 1 g/kg or 3 g/kg ethanol relative to control ( n = 6–8/group), as assessed by in situ hybridization histochemistry with digoxigenin-labeled

    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000; Roche Diagnostics, Indianapolis, IN, USA) and NBT/BCIP (Roche Diagnostics) were used to visualize the signal.

    Techniques: Expressing, In Situ Hybridization, Labeling

    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with DIG-Stx1B plus HRP-anti-DIG antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.

    Journal: Infection and Immunity

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon

    doi: 10.1128/IAI.71.2.985-990.2003

    Figure Lengend Snippet: Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with DIG-Stx1B plus HRP-anti-DIG antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.

    Article Snippet: Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Techniques: Thin Layer Chromatography, Staining, Purification

    Comparison of binding with digoxigenin-conjugated MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.

    Journal: Virology Journal

    Article Title: Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution

    doi: 10.1186/1743-422X-7-38

    Figure Lengend Snippet: Comparison of binding with digoxigenin-conjugated MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.

    Article Snippet: Subsequently, the slides were incubated with 1:200 sheep alkaline phosphatase conjugated anti-digoxigenin Fab fragments (Roche) and developed with New Fuchsin (Dako).

    Techniques: Binding Assay

    Influence of the conjugation method of MAL-I and -II lectins on the staining intensities in duck small intestines . Biotinylated MAL-I (A) and MAL-II (a) both resulted in epithelial cell binding (black arrows), but MAL-II (a) was additionally staining the goblet cells (red arrow). For both lectins binding was abolished by sialidase treatment of the sections (B, b). Digoxigenin labelled MAL-I (C) and MAL-II (c) failed to bind to the same tissues.

    Journal: Virology Journal

    Article Title: Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution

    doi: 10.1186/1743-422X-7-38

    Figure Lengend Snippet: Influence of the conjugation method of MAL-I and -II lectins on the staining intensities in duck small intestines . Biotinylated MAL-I (A) and MAL-II (a) both resulted in epithelial cell binding (black arrows), but MAL-II (a) was additionally staining the goblet cells (red arrow). For both lectins binding was abolished by sialidase treatment of the sections (B, b). Digoxigenin labelled MAL-I (C) and MAL-II (c) failed to bind to the same tissues.

    Article Snippet: Subsequently, the slides were incubated with 1:200 sheep alkaline phosphatase conjugated anti-digoxigenin Fab fragments (Roche) and developed with New Fuchsin (Dako).

    Techniques: Conjugation Assay, Staining, Binding Assay