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sheath fluid  (Bio-Rad)


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    Bio-Rad sheath fluid
    Sheath Fluid, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheath+fluid/Instrument+Sheath+Fluid/pm41183607-81-0-22
    Average 93 stars, based on 61 article reviews
    sheath fluid - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Targeting Langerhans cells via skin delivery of HIV Envelope enhances the antibody response to vaccination
    Article Snippet: The beads, suspended in 80 μl of Sheath fluid (Bio-Rad), were agitated for 5 minutes at 700 rpm on a plate shaker before being directly read on a Bioplex-200 plate reader (Bio-Rad) with an acquisition volume of 50 μl and DD gate settings of 5000–25,000.

    Article Title: Impact of Pro-Inflammatory Cytokine Preconditioning on Metabolism and Extracellular Vesicles in Feline Mesenchymal Stromal Cells: A Preliminary Study.
    Article Snippet: Impact of Pro-Inflammatory Cytokine Preconditioning on Metabolism and Extracellular Vesicles in Feline Mesenchymal Stromal Cells: A Preliminary Study Maria Soltero-Rivera, Boaz Arzi, Lynda Bourebaba, Krzysztof Marycz D ow nloaded from https://academ ic.oup.com /stm cls/advance-article/doi/10.1093/stm cls/sxaf014/8102800 by guest on 07 April 2025 Ac ce pte d M an us cri pt © The Author(s) 2025.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons AttributionNonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits noncommercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Impact of laboratory technician experience on the accuracy of laboratory test results in scientific research.
    Article Snippet: Sheath Fluid is added to the remaining components, and the plate is fluorometrically measured in the Bio-Plex® 200TM (The Bio-Plex 200 system, Bio-Rad Laboratories, Inc).

    Article Title: Single-cell eQTL mapping in yeast reveals a tradeoff between growth and reproduction
    Article Snippet: 1 ml 2× YNB with 2% glucose was added to the sorted cells in sheath fluid (PBS, Bio-Rad #12012932) and they were transferred to a shaking incubator and grown at 30°C.

    Article Title: Impact of pro-inflammatory cytokine preconditioning on metabolism and extracellular vesicles in feline mesenchymal stromal cells: a preliminary study
    Article Snippet: The plate was washed 3 times, and 150 μL of 1× Sheath fluid (Bio-Rad Laboratories Inc, Hercules, CA) was added to each well.

    Suspension:

    Article Title: Prenatal alcohol exposure worsens acute but not long‐term cognitive outcomes due to stroke in middle‐aged Sprague–Dawley rat offspring
    Article Snippet: .. After two washes, beads were resuspended in 150 μ L of sheath fluid and a minimum of 50 beads per analyte was analyzed in a Bio‐Plex suspension array system (Bio‐Rad Laboratories, CA). ..

    Incubation:

    Article Title: Characterization of the smoltification process and post-sea transfer stunts of Atlantic salmon (salmo salar) in full-scale intensive production
    Article Snippet: .. Subsequently, the assay plate was washed twice, 100 μL of sheath fluid (Bio-Rad Norway AS, Oslo, Norway) was added to all wells, and the plate was incubated on a plate shaker for 5 min as described above to resuspend the beads before analysis. .. The Bio-Plex 3D suspension array system (Luminex Corporation, USA) and Bio-Plex 200 system (Bio-Rad) were used to acquire data, followed by data analysis using the xPONENT® software (Luminex) and the Bio-Plex Manager software (version 6.2), respectively.



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    Image Search Results


    Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Journal: bioRxiv

    Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

    doi: 10.1101/2025.11.13.688239

    Figure Lengend Snippet: Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

    Techniques: Sequencing, Extraction, DNA Extraction, SYBR Green Assay, Staining

    The removal of relic DNA during sorting resulted in discrepancies between the non-sorted and sorted samples. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Journal: bioRxiv

    Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

    doi: 10.1101/2025.11.13.688239

    Figure Lengend Snippet: The removal of relic DNA during sorting resulted in discrepancies between the non-sorted and sorted samples. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. The low biomass was obtained through dilution of the high biomass mock standard (10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction) and after (diluted post-extraction) DNA extraction and through sorting of the mock standard into 10 5 cells mL -1 and 10 5 noise events mL -1 using a non-selective SYBR® Green I staining. Dilution and sorting were performed in triplicate. FACS sheath fluid and aPBS diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

    Techniques: Sequencing, Extraction, DNA Extraction, SYBR Green Assay, Staining

    Low biomass samples obtained through dilution of the high biomass mock standard (orange; 10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction; turquoise) and after (diluted post-extraction; green) DNA extraction and sorted noise events (10 5 mL -1 ; purple) did not display an improved clustering. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Dilution and sorting with a non-selective SYBR® Green I cell staining were performed in triplicate. FACS sheath fluid (light grey) and aPBS (brown) diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Journal: bioRxiv

    Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

    doi: 10.1101/2025.11.13.688239

    Figure Lengend Snippet: Low biomass samples obtained through dilution of the high biomass mock standard (orange; 10 7 cells mL -1 ) to 10 5 cells mL -1 before (diluted pre-extraction; turquoise) and after (diluted post-extraction; green) DNA extraction and sorted noise events (10 5 mL -1 ; purple) did not display an improved clustering. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Dilution and sorting with a non-selective SYBR® Green I cell staining were performed in triplicate. FACS sheath fluid (light grey) and aPBS (brown) diluent blanks were included in duplicate to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

    Techniques: Extraction, DNA Extraction, Sequencing, SYBR Green Assay, Staining

    Benzonase nuclease activity selectively depleted relic DNA of Veillonella atypica , Pseudomonas aeruginosa ASV63, Escherichia - Shigella coli ASV 11/42/43/77/88/179 and Salmonella enterica ASV 25/201. AMPure XP clean-up eliminated relic DNA but was not selective resulting in a distorted community composition. A decrease in the (relic) DNA concentration prior to sorting resulted in an inflation in absolute and relative contaminant sequencing read counts attesting the need of in silico data decontamination. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Cells were sorted using a non-selective SYBR® Green I staining. A FACS sheath fluid blank was included to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Journal: bioRxiv

    Article Title: An optimized flow cytometry sorting-sequencing workflow reduces storage, sorting and extraction bias in sorted microbial communities

    doi: 10.1101/2025.11.13.688239

    Figure Lengend Snippet: Benzonase nuclease activity selectively depleted relic DNA of Veillonella atypica , Pseudomonas aeruginosa ASV63, Escherichia - Shigella coli ASV 11/42/43/77/88/179 and Salmonella enterica ASV 25/201. AMPure XP clean-up eliminated relic DNA but was not selective resulting in a distorted community composition. A decrease in the (relic) DNA concentration prior to sorting resulted in an inflation in absolute and relative contaminant sequencing read counts attesting the need of in silico data decontamination. Samples were sequenced with PacBio full-length 16S rRNA gene sequencing. Cells were sorted using a non-selective SYBR® Green I staining. A FACS sheath fluid blank was included to identify and track the FACSome contaminants introduced during FACS-sequencing.

    Article Snippet: The sorted cell fraction consisted of 288,000 cells collected in 1 mL FACS sheath fluid in 1.5 mL Eppendorf® DNA LoBind collection tubes (Merck, Darmstadt, Germany).

    Techniques: Activity Assay, Concentration Assay, Sequencing, In Silico, SYBR Green Assay, Staining