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Santa Cruz Biotechnology sgk3 sirna duplexes
Androgen/AR-dependent <t>SGK3</t> transcription involves ER. A, LNCaP cells were transfected with the <t>siRNA</t> negative control (siNC) or ERβ siRNA (siERβ) for 72 hours. Cell extracts were subjected to Western blotting. B, After being hormone-stripped for 2 days, LNCaP cells were transfected with the siRNA negative control or ERβ siRNA for 48 hours and then were transfected with the luciferase reporters as indicated and cultured in the presence or absence of 1 nM DHT for 24 hours. Luciferase activity of cell extracts was measured and normalized to protein concentration. Data are expressed as means ± SD from 3 independent experiments. **, P
Sgk3 Sirna Duplexes, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "SGK3 Is an Androgen-Inducible Kinase Promoting Prostate Cancer Cell Proliferation Through Activation of p70 S6 Kinase and Up-Regulation of Cyclin D1"

Article Title: SGK3 Is an Androgen-Inducible Kinase Promoting Prostate Cancer Cell Proliferation Through Activation of p70 S6 Kinase and Up-Regulation of Cyclin D1

Journal: Molecular Endocrinology

doi: 10.1210/me.2013-1339

Androgen/AR-dependent SGK3 transcription involves ER. A, LNCaP cells were transfected with the siRNA negative control (siNC) or ERβ siRNA (siERβ) for 72 hours. Cell extracts were subjected to Western blotting. B, After being hormone-stripped for 2 days, LNCaP cells were transfected with the siRNA negative control or ERβ siRNA for 48 hours and then were transfected with the luciferase reporters as indicated and cultured in the presence or absence of 1 nM DHT for 24 hours. Luciferase activity of cell extracts was measured and normalized to protein concentration. Data are expressed as means ± SD from 3 independent experiments. **, P
Figure Legend Snippet: Androgen/AR-dependent SGK3 transcription involves ER. A, LNCaP cells were transfected with the siRNA negative control (siNC) or ERβ siRNA (siERβ) for 72 hours. Cell extracts were subjected to Western blotting. B, After being hormone-stripped for 2 days, LNCaP cells were transfected with the siRNA negative control or ERβ siRNA for 48 hours and then were transfected with the luciferase reporters as indicated and cultured in the presence or absence of 1 nM DHT for 24 hours. Luciferase activity of cell extracts was measured and normalized to protein concentration. Data are expressed as means ± SD from 3 independent experiments. **, P

Techniques Used: Transfection, Negative Control, Western Blot, Luciferase, Cell Culture, Activity Assay, Protein Concentration

SGK3 promotes cell proliferation of AR-positive prostate cancer cells. A and B, LNCaP cells (A) or 22Rv1 cells (B) were transfected with the siRNA negative control (siNC) or 3 individual SGK3 siRNA duplexes (siSGK), respectively. Western blotting (insert) was performed to examine SGK3 level after the cells were transfected with siRNA for 72 hours. An MTT assay was performed to measure cell proliferation at the indicated time after siRNA transfection. Error bars represent SDs of 3 independent experiments. C, LNCaP/TetON/SGK3 cells cultured in phenol red–free RPMI 1640 medium with 2% CD-treated FBS were added with or without 100 ng/mL DOX. An MTT assay was performed to measure cell proliferation at the indicated time. D, LNCaP cells were transfected with the siRNA negative control or SGK3 siRNA and cultured in the phenol red–free RPMI 1640 medium with 5% CD-treated FBS in the presence or absence of DHT (1 nM). SGK3 protein levels were examined by Western blotting at 72 hours after siRNA transfection. The cell growth rate was measured by an MTT assay at the indicated time. **, P
Figure Legend Snippet: SGK3 promotes cell proliferation of AR-positive prostate cancer cells. A and B, LNCaP cells (A) or 22Rv1 cells (B) were transfected with the siRNA negative control (siNC) or 3 individual SGK3 siRNA duplexes (siSGK), respectively. Western blotting (insert) was performed to examine SGK3 level after the cells were transfected with siRNA for 72 hours. An MTT assay was performed to measure cell proliferation at the indicated time after siRNA transfection. Error bars represent SDs of 3 independent experiments. C, LNCaP/TetON/SGK3 cells cultured in phenol red–free RPMI 1640 medium with 2% CD-treated FBS were added with or without 100 ng/mL DOX. An MTT assay was performed to measure cell proliferation at the indicated time. D, LNCaP cells were transfected with the siRNA negative control or SGK3 siRNA and cultured in the phenol red–free RPMI 1640 medium with 5% CD-treated FBS in the presence or absence of DHT (1 nM). SGK3 protein levels were examined by Western blotting at 72 hours after siRNA transfection. The cell growth rate was measured by an MTT assay at the indicated time. **, P

Techniques Used: Transfection, Negative Control, Western Blot, MTT Assay, Cell Culture

Knockdown of SGK3 expression results in a decrease in p70S6K phosphorylation. A and B, LNCaP cells were transfected with the siRNA negative control (siNC), pooled (A) or individual (B) SGK3 siRNA duplexes (siSGK3), respectively. At 72 hours after transfection, the cells were harvested for Western blotting analysis with the relevant antibodies. C and D, LNCaP cells were treated with DMSO or the indicated concentrations of rapamycin (RAPA). An MTT assay (D) was performed to measure cell proliferation at the indicated time posttreatment. C, Western blotting was performed at 24 hours after treatment. **, P
Figure Legend Snippet: Knockdown of SGK3 expression results in a decrease in p70S6K phosphorylation. A and B, LNCaP cells were transfected with the siRNA negative control (siNC), pooled (A) or individual (B) SGK3 siRNA duplexes (siSGK3), respectively. At 72 hours after transfection, the cells were harvested for Western blotting analysis with the relevant antibodies. C and D, LNCaP cells were treated with DMSO or the indicated concentrations of rapamycin (RAPA). An MTT assay (D) was performed to measure cell proliferation at the indicated time posttreatment. C, Western blotting was performed at 24 hours after treatment. **, P

Techniques Used: Expressing, Transfection, Negative Control, Western Blot, MTT Assay

SGK3 promotes the G 1 to S phase cell cycle progression of LNCaP cells through up-regulation of cyclin D1. A and B, LNCaP cells were transfected with the siRNA negative control (siNC) or pooled SGK3 siRNA duplexes (siSGK3) for 4 days. The transfected cells were imaged under a microscope (A) or harvested for Western blotting (B) to determine PARP cleavage and cleaved caspase 3 levels. Scale bar corresponds to 50 μm; ×100 magnification. For Western blotting analysis of apoptosis markers, we also included a positive control, which are LNCaP cells treated with 20 μM proteasome inhibitor MG132 for 24 hours. C, LNCaP cells were transfected with siRNA negative control (LNCaP/siNC) or pooled SGK3 siRNA duplexes (LNCaP/siSGK3). Seventy-two hours after transfection, cells were harvested, fixed in 70% ethanol, and stained with propidium iodide and analyzed by flow cytometry. The percentage of DNA content in each phase was analyzed with ModFit LT 2.0 software. The representative result of 3 experiments was shown. D, Quantitative result of the percentage of DNA content in each cell cycle phase from 3 experiments. The data are expressed as means ± SD. *, P
Figure Legend Snippet: SGK3 promotes the G 1 to S phase cell cycle progression of LNCaP cells through up-regulation of cyclin D1. A and B, LNCaP cells were transfected with the siRNA negative control (siNC) or pooled SGK3 siRNA duplexes (siSGK3) for 4 days. The transfected cells were imaged under a microscope (A) or harvested for Western blotting (B) to determine PARP cleavage and cleaved caspase 3 levels. Scale bar corresponds to 50 μm; ×100 magnification. For Western blotting analysis of apoptosis markers, we also included a positive control, which are LNCaP cells treated with 20 μM proteasome inhibitor MG132 for 24 hours. C, LNCaP cells were transfected with siRNA negative control (LNCaP/siNC) or pooled SGK3 siRNA duplexes (LNCaP/siSGK3). Seventy-two hours after transfection, cells were harvested, fixed in 70% ethanol, and stained with propidium iodide and analyzed by flow cytometry. The percentage of DNA content in each phase was analyzed with ModFit LT 2.0 software. The representative result of 3 experiments was shown. D, Quantitative result of the percentage of DNA content in each cell cycle phase from 3 experiments. The data are expressed as means ± SD. *, P

Techniques Used: Transfection, Negative Control, Microscopy, Western Blot, Positive Control, Staining, Flow Cytometry, Cytometry, Software

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Article Title: SGK3 Is an Androgen-Inducible Kinase Promoting Prostate Cancer Cell Proliferation Through Activation of p70 S6 Kinase and Up-Regulation of Cyclin D1
Article Snippet: .. To this end, LNCaP cells were transfected with either pooled (sc-44852; Santa Cruz Biotechnology) or 3 individual SGK3 siRNA duplexes (sc-44852A, sc-44852B, and sc-44852C; Santa Cruz Biotechnology). .. As shown in A and , transfection with either pooled SGK3 siRNA or 3 individual SGK3 siRNA duplexes resulted in a dramatic decrease in SGK3 expression and a significant suppression of cell proliferation.

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    Santa Cruz Biotechnology sgk3 sirna duplexes
    Androgen/AR-dependent <t>SGK3</t> transcription involves ER. A, LNCaP cells were transfected with the <t>siRNA</t> negative control (siNC) or ERβ siRNA (siERβ) for 72 hours. Cell extracts were subjected to Western blotting. B, After being hormone-stripped for 2 days, LNCaP cells were transfected with the siRNA negative control or ERβ siRNA for 48 hours and then were transfected with the luciferase reporters as indicated and cultured in the presence or absence of 1 nM DHT for 24 hours. Luciferase activity of cell extracts was measured and normalized to protein concentration. Data are expressed as means ± SD from 3 independent experiments. **, P
    Sgk3 Sirna Duplexes, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sgk3 sirna duplexes/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sgk3 sirna duplexes - by Bioz Stars, 2020-09
    86/100 stars
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    Androgen/AR-dependent SGK3 transcription involves ER. A, LNCaP cells were transfected with the siRNA negative control (siNC) or ERβ siRNA (siERβ) for 72 hours. Cell extracts were subjected to Western blotting. B, After being hormone-stripped for 2 days, LNCaP cells were transfected with the siRNA negative control or ERβ siRNA for 48 hours and then were transfected with the luciferase reporters as indicated and cultured in the presence or absence of 1 nM DHT for 24 hours. Luciferase activity of cell extracts was measured and normalized to protein concentration. Data are expressed as means ± SD from 3 independent experiments. **, P

    Journal: Molecular Endocrinology

    Article Title: SGK3 Is an Androgen-Inducible Kinase Promoting Prostate Cancer Cell Proliferation Through Activation of p70 S6 Kinase and Up-Regulation of Cyclin D1

    doi: 10.1210/me.2013-1339

    Figure Lengend Snippet: Androgen/AR-dependent SGK3 transcription involves ER. A, LNCaP cells were transfected with the siRNA negative control (siNC) or ERβ siRNA (siERβ) for 72 hours. Cell extracts were subjected to Western blotting. B, After being hormone-stripped for 2 days, LNCaP cells were transfected with the siRNA negative control or ERβ siRNA for 48 hours and then were transfected with the luciferase reporters as indicated and cultured in the presence or absence of 1 nM DHT for 24 hours. Luciferase activity of cell extracts was measured and normalized to protein concentration. Data are expressed as means ± SD from 3 independent experiments. **, P

    Article Snippet: To this end, LNCaP cells were transfected with either pooled (sc-44852; Santa Cruz Biotechnology) or 3 individual SGK3 siRNA duplexes (sc-44852A, sc-44852B, and sc-44852C; Santa Cruz Biotechnology).

    Techniques: Transfection, Negative Control, Western Blot, Luciferase, Cell Culture, Activity Assay, Protein Concentration

    SGK3 promotes cell proliferation of AR-positive prostate cancer cells. A and B, LNCaP cells (A) or 22Rv1 cells (B) were transfected with the siRNA negative control (siNC) or 3 individual SGK3 siRNA duplexes (siSGK), respectively. Western blotting (insert) was performed to examine SGK3 level after the cells were transfected with siRNA for 72 hours. An MTT assay was performed to measure cell proliferation at the indicated time after siRNA transfection. Error bars represent SDs of 3 independent experiments. C, LNCaP/TetON/SGK3 cells cultured in phenol red–free RPMI 1640 medium with 2% CD-treated FBS were added with or without 100 ng/mL DOX. An MTT assay was performed to measure cell proliferation at the indicated time. D, LNCaP cells were transfected with the siRNA negative control or SGK3 siRNA and cultured in the phenol red–free RPMI 1640 medium with 5% CD-treated FBS in the presence or absence of DHT (1 nM). SGK3 protein levels were examined by Western blotting at 72 hours after siRNA transfection. The cell growth rate was measured by an MTT assay at the indicated time. **, P

    Journal: Molecular Endocrinology

    Article Title: SGK3 Is an Androgen-Inducible Kinase Promoting Prostate Cancer Cell Proliferation Through Activation of p70 S6 Kinase and Up-Regulation of Cyclin D1

    doi: 10.1210/me.2013-1339

    Figure Lengend Snippet: SGK3 promotes cell proliferation of AR-positive prostate cancer cells. A and B, LNCaP cells (A) or 22Rv1 cells (B) were transfected with the siRNA negative control (siNC) or 3 individual SGK3 siRNA duplexes (siSGK), respectively. Western blotting (insert) was performed to examine SGK3 level after the cells were transfected with siRNA for 72 hours. An MTT assay was performed to measure cell proliferation at the indicated time after siRNA transfection. Error bars represent SDs of 3 independent experiments. C, LNCaP/TetON/SGK3 cells cultured in phenol red–free RPMI 1640 medium with 2% CD-treated FBS were added with or without 100 ng/mL DOX. An MTT assay was performed to measure cell proliferation at the indicated time. D, LNCaP cells were transfected with the siRNA negative control or SGK3 siRNA and cultured in the phenol red–free RPMI 1640 medium with 5% CD-treated FBS in the presence or absence of DHT (1 nM). SGK3 protein levels were examined by Western blotting at 72 hours after siRNA transfection. The cell growth rate was measured by an MTT assay at the indicated time. **, P

    Article Snippet: To this end, LNCaP cells were transfected with either pooled (sc-44852; Santa Cruz Biotechnology) or 3 individual SGK3 siRNA duplexes (sc-44852A, sc-44852B, and sc-44852C; Santa Cruz Biotechnology).

    Techniques: Transfection, Negative Control, Western Blot, MTT Assay, Cell Culture

    Knockdown of SGK3 expression results in a decrease in p70S6K phosphorylation. A and B, LNCaP cells were transfected with the siRNA negative control (siNC), pooled (A) or individual (B) SGK3 siRNA duplexes (siSGK3), respectively. At 72 hours after transfection, the cells were harvested for Western blotting analysis with the relevant antibodies. C and D, LNCaP cells were treated with DMSO or the indicated concentrations of rapamycin (RAPA). An MTT assay (D) was performed to measure cell proliferation at the indicated time posttreatment. C, Western blotting was performed at 24 hours after treatment. **, P

    Journal: Molecular Endocrinology

    Article Title: SGK3 Is an Androgen-Inducible Kinase Promoting Prostate Cancer Cell Proliferation Through Activation of p70 S6 Kinase and Up-Regulation of Cyclin D1

    doi: 10.1210/me.2013-1339

    Figure Lengend Snippet: Knockdown of SGK3 expression results in a decrease in p70S6K phosphorylation. A and B, LNCaP cells were transfected with the siRNA negative control (siNC), pooled (A) or individual (B) SGK3 siRNA duplexes (siSGK3), respectively. At 72 hours after transfection, the cells were harvested for Western blotting analysis with the relevant antibodies. C and D, LNCaP cells were treated with DMSO or the indicated concentrations of rapamycin (RAPA). An MTT assay (D) was performed to measure cell proliferation at the indicated time posttreatment. C, Western blotting was performed at 24 hours after treatment. **, P

    Article Snippet: To this end, LNCaP cells were transfected with either pooled (sc-44852; Santa Cruz Biotechnology) or 3 individual SGK3 siRNA duplexes (sc-44852A, sc-44852B, and sc-44852C; Santa Cruz Biotechnology).

    Techniques: Expressing, Transfection, Negative Control, Western Blot, MTT Assay

    SGK3 promotes the G 1 to S phase cell cycle progression of LNCaP cells through up-regulation of cyclin D1. A and B, LNCaP cells were transfected with the siRNA negative control (siNC) or pooled SGK3 siRNA duplexes (siSGK3) for 4 days. The transfected cells were imaged under a microscope (A) or harvested for Western blotting (B) to determine PARP cleavage and cleaved caspase 3 levels. Scale bar corresponds to 50 μm; ×100 magnification. For Western blotting analysis of apoptosis markers, we also included a positive control, which are LNCaP cells treated with 20 μM proteasome inhibitor MG132 for 24 hours. C, LNCaP cells were transfected with siRNA negative control (LNCaP/siNC) or pooled SGK3 siRNA duplexes (LNCaP/siSGK3). Seventy-two hours after transfection, cells were harvested, fixed in 70% ethanol, and stained with propidium iodide and analyzed by flow cytometry. The percentage of DNA content in each phase was analyzed with ModFit LT 2.0 software. The representative result of 3 experiments was shown. D, Quantitative result of the percentage of DNA content in each cell cycle phase from 3 experiments. The data are expressed as means ± SD. *, P

    Journal: Molecular Endocrinology

    Article Title: SGK3 Is an Androgen-Inducible Kinase Promoting Prostate Cancer Cell Proliferation Through Activation of p70 S6 Kinase and Up-Regulation of Cyclin D1

    doi: 10.1210/me.2013-1339

    Figure Lengend Snippet: SGK3 promotes the G 1 to S phase cell cycle progression of LNCaP cells through up-regulation of cyclin D1. A and B, LNCaP cells were transfected with the siRNA negative control (siNC) or pooled SGK3 siRNA duplexes (siSGK3) for 4 days. The transfected cells were imaged under a microscope (A) or harvested for Western blotting (B) to determine PARP cleavage and cleaved caspase 3 levels. Scale bar corresponds to 50 μm; ×100 magnification. For Western blotting analysis of apoptosis markers, we also included a positive control, which are LNCaP cells treated with 20 μM proteasome inhibitor MG132 for 24 hours. C, LNCaP cells were transfected with siRNA negative control (LNCaP/siNC) or pooled SGK3 siRNA duplexes (LNCaP/siSGK3). Seventy-two hours after transfection, cells were harvested, fixed in 70% ethanol, and stained with propidium iodide and analyzed by flow cytometry. The percentage of DNA content in each phase was analyzed with ModFit LT 2.0 software. The representative result of 3 experiments was shown. D, Quantitative result of the percentage of DNA content in each cell cycle phase from 3 experiments. The data are expressed as means ± SD. *, P

    Article Snippet: To this end, LNCaP cells were transfected with either pooled (sc-44852; Santa Cruz Biotechnology) or 3 individual SGK3 siRNA duplexes (sc-44852A, sc-44852B, and sc-44852C; Santa Cruz Biotechnology).

    Techniques: Transfection, Negative Control, Microscopy, Western Blot, Positive Control, Staining, Flow Cytometry, Cytometry, Software