sf767 glioma cell line  (Roche)


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    Roche sf767 glioma cell line
    Knockdown of MAP4K4 expression inhibits glioma cell migration and invasion and blocks Pyk2 mediated stimulation of migration. (a) <t>SF767</t> cells were transiently transfected with shRNA 1M4K4i and shRNA 2M4K4i or cotransfected with shRNAs 1M4K41i and 2M4K4i. Cell lysates were blotted with indicated antibodies. (b) SF767 cells were stably transduced with lentivirus expressing 2M4K4i shRNA. Stably transduced cells (solid histogram) were enriched by flow cytometry. Open histogram: control SF767 parental cells. Inset: whole cell lysates of control SF767 cells or SF767 cells stably transduced with MAP4K4 shRNA (MAP4K4i) were immunoblotted with the indicated antibodies. (c) The migration rate of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), and MAP4K4i cells transfected with Pyk2 was assayed with a radial migration assay on 10 μ g/mL laminin substrate. * P
    Sf767 Glioma Cell Line, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sf767 glioma cell line/product/Roche
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    sf767 glioma cell line - by Bioz Stars, 2020-09
    85/100 stars

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    Images

    1) Product Images from "A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration"

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration

    Journal: Journal of Signal Transduction

    doi: 10.1155/2013/956580

    Knockdown of MAP4K4 expression inhibits glioma cell migration and invasion and blocks Pyk2 mediated stimulation of migration. (a) SF767 cells were transiently transfected with shRNA 1M4K4i and shRNA 2M4K4i or cotransfected with shRNAs 1M4K41i and 2M4K4i. Cell lysates were blotted with indicated antibodies. (b) SF767 cells were stably transduced with lentivirus expressing 2M4K4i shRNA. Stably transduced cells (solid histogram) were enriched by flow cytometry. Open histogram: control SF767 parental cells. Inset: whole cell lysates of control SF767 cells or SF767 cells stably transduced with MAP4K4 shRNA (MAP4K4i) were immunoblotted with the indicated antibodies. (c) The migration rate of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), and MAP4K4i cells transfected with Pyk2 was assayed with a radial migration assay on 10 μ g/mL laminin substrate. * P
    Figure Legend Snippet: Knockdown of MAP4K4 expression inhibits glioma cell migration and invasion and blocks Pyk2 mediated stimulation of migration. (a) SF767 cells were transiently transfected with shRNA 1M4K4i and shRNA 2M4K4i or cotransfected with shRNAs 1M4K41i and 2M4K4i. Cell lysates were blotted with indicated antibodies. (b) SF767 cells were stably transduced with lentivirus expressing 2M4K4i shRNA. Stably transduced cells (solid histogram) were enriched by flow cytometry. Open histogram: control SF767 parental cells. Inset: whole cell lysates of control SF767 cells or SF767 cells stably transduced with MAP4K4 shRNA (MAP4K4i) were immunoblotted with the indicated antibodies. (c) The migration rate of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), and MAP4K4i cells transfected with Pyk2 was assayed with a radial migration assay on 10 μ g/mL laminin substrate. * P

    Techniques Used: Expressing, Migration, Transfection, shRNA, Stable Transfection, Transduction, Flow Cytometry, Cytometry

    Schematic of MAP4K4 and interacting two-hybrid clones. (a) Structure of full length MAP4K4 indicating the N-terminal kinase domain, the C-terminal citron homology domain (CNH), and the location of alternatively spliced modules M1–M9. (b) Structure of the three overlapping clones isolated in the yeast two-hybrid genetic screen which interact with the Pyk2 FERM domain. (c) Structure of the final MAP4K4 clone assembled from SF767 glioma cell isoforms.
    Figure Legend Snippet: Schematic of MAP4K4 and interacting two-hybrid clones. (a) Structure of full length MAP4K4 indicating the N-terminal kinase domain, the C-terminal citron homology domain (CNH), and the location of alternatively spliced modules M1–M9. (b) Structure of the three overlapping clones isolated in the yeast two-hybrid genetic screen which interact with the Pyk2 FERM domain. (c) Structure of the final MAP4K4 clone assembled from SF767 glioma cell isoforms.

    Techniques Used: Clone Assay, Isolation

    Pyk2 coimmunoprecipitates with MAP4K4. (a) Cells cotransfected with FLAG-epitope-tagged Pyk2 FERM and HA-epitope-tagged MAP4K4(143) were lysed and immunoprecipitated with anti-FLAG antibody or normal mouse IgG. Immunoprecipitates were immunoblotted (IB) with anti-FLAG or anti-HA. WCL: whole cell lysate. (b) Cells were cotransfected with FLAG-epitope-tagged MAP4K4(143) and either HA-tagged Pyk2 or HA-tagged FAK, lysed, and immunoprecipitated with mouse IgG or anti-FLAG antibody. Immunoprecipitates or WCL were immunoblotted as indicated. (c) Cells cotransfected with FLAG-tagged Pyk2 and either HA-tagged MAP4K4(143) or HA-tagged full length (FL) MAP4K4 were lysed and immunoprecipitated with rabbit IgG or anti-HA antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies. (d) SF767 glioma cells were lysed and immunoprecipitated with anti-MAP4K4 antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies.
    Figure Legend Snippet: Pyk2 coimmunoprecipitates with MAP4K4. (a) Cells cotransfected with FLAG-epitope-tagged Pyk2 FERM and HA-epitope-tagged MAP4K4(143) were lysed and immunoprecipitated with anti-FLAG antibody or normal mouse IgG. Immunoprecipitates were immunoblotted (IB) with anti-FLAG or anti-HA. WCL: whole cell lysate. (b) Cells were cotransfected with FLAG-epitope-tagged MAP4K4(143) and either HA-tagged Pyk2 or HA-tagged FAK, lysed, and immunoprecipitated with mouse IgG or anti-FLAG antibody. Immunoprecipitates or WCL were immunoblotted as indicated. (c) Cells cotransfected with FLAG-tagged Pyk2 and either HA-tagged MAP4K4(143) or HA-tagged full length (FL) MAP4K4 were lysed and immunoprecipitated with rabbit IgG or anti-HA antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies. (d) SF767 glioma cells were lysed and immunoprecipitated with anti-MAP4K4 antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies.

    Techniques Used: FLAG-tag, Immunoprecipitation

    Increased expression of MAP4K4 stimulates migration but is blocked by knockdown of Pyk2 expression. (a) Wild-type SF767 glioma cells (CTL) or SF767 cells stably transduced with a shRNA targeting Pyk2 (Pyk2i) were transfected with vector or HA-epitope-tagged MAP4K4. Whole cell lysates were blotted with the indicated antibodies. Lysates were blotted with actin as a loading control. (b) SF767 control cells or SF767 cells with shRNA mediated knockdown of Pyk2 (Pyk2i) were transfected with MAP4K4, and the migration rate on 10 μ g/mL laminin was assessed over 24 hr using a radial migration assay. † P = 0.0016 relative to control. * P
    Figure Legend Snippet: Increased expression of MAP4K4 stimulates migration but is blocked by knockdown of Pyk2 expression. (a) Wild-type SF767 glioma cells (CTL) or SF767 cells stably transduced with a shRNA targeting Pyk2 (Pyk2i) were transfected with vector or HA-epitope-tagged MAP4K4. Whole cell lysates were blotted with the indicated antibodies. Lysates were blotted with actin as a loading control. (b) SF767 control cells or SF767 cells with shRNA mediated knockdown of Pyk2 (Pyk2i) were transfected with MAP4K4, and the migration rate on 10 μ g/mL laminin was assessed over 24 hr using a radial migration assay. † P = 0.0016 relative to control. * P

    Techniques Used: Expressing, Migration, CTL Assay, Stable Transfection, Transduction, shRNA, Transfection, Plasmid Preparation

    Related Articles

    Sequencing:

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration
    Article Snippet: .. The coding sequence for full length MAP4K4 was isolated by RT-PCR of total RNA isolated from the SF767 glioma cell line using the Titan RT-PCR kit (Roche Applied Science, Indianapolis, IN) according to the manufacturer's instructions. ..

    Isolation:

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration
    Article Snippet: .. The coding sequence for full length MAP4K4 was isolated by RT-PCR of total RNA isolated from the SF767 glioma cell line using the Titan RT-PCR kit (Roche Applied Science, Indianapolis, IN) according to the manufacturer's instructions. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration
    Article Snippet: .. The coding sequence for full length MAP4K4 was isolated by RT-PCR of total RNA isolated from the SF767 glioma cell line using the Titan RT-PCR kit (Roche Applied Science, Indianapolis, IN) according to the manufacturer's instructions. ..

    Similar Products

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  • 85
    Roche sf767 glioma cell line
    Knockdown of MAP4K4 expression inhibits glioma cell migration and invasion and blocks Pyk2 mediated stimulation of migration. (a) <t>SF767</t> cells were transiently transfected with shRNA 1M4K4i and shRNA 2M4K4i or cotransfected with shRNAs 1M4K41i and 2M4K4i. Cell lysates were blotted with indicated antibodies. (b) SF767 cells were stably transduced with lentivirus expressing 2M4K4i shRNA. Stably transduced cells (solid histogram) were enriched by flow cytometry. Open histogram: control SF767 parental cells. Inset: whole cell lysates of control SF767 cells or SF767 cells stably transduced with MAP4K4 shRNA (MAP4K4i) were immunoblotted with the indicated antibodies. (c) The migration rate of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), and MAP4K4i cells transfected with Pyk2 was assayed with a radial migration assay on 10 μ g/mL laminin substrate. * P
    Sf767 Glioma Cell Line, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sf767 glioma cell line/product/Roche
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    sf767 glioma cell line - by Bioz Stars, 2020-09
    85/100 stars
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    Knockdown of MAP4K4 expression inhibits glioma cell migration and invasion and blocks Pyk2 mediated stimulation of migration. (a) SF767 cells were transiently transfected with shRNA 1M4K4i and shRNA 2M4K4i or cotransfected with shRNAs 1M4K41i and 2M4K4i. Cell lysates were blotted with indicated antibodies. (b) SF767 cells were stably transduced with lentivirus expressing 2M4K4i shRNA. Stably transduced cells (solid histogram) were enriched by flow cytometry. Open histogram: control SF767 parental cells. Inset: whole cell lysates of control SF767 cells or SF767 cells stably transduced with MAP4K4 shRNA (MAP4K4i) were immunoblotted with the indicated antibodies. (c) The migration rate of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), and MAP4K4i cells transfected with Pyk2 was assayed with a radial migration assay on 10 μ g/mL laminin substrate. * P

    Journal: Journal of Signal Transduction

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration

    doi: 10.1155/2013/956580

    Figure Lengend Snippet: Knockdown of MAP4K4 expression inhibits glioma cell migration and invasion and blocks Pyk2 mediated stimulation of migration. (a) SF767 cells were transiently transfected with shRNA 1M4K4i and shRNA 2M4K4i or cotransfected with shRNAs 1M4K41i and 2M4K4i. Cell lysates were blotted with indicated antibodies. (b) SF767 cells were stably transduced with lentivirus expressing 2M4K4i shRNA. Stably transduced cells (solid histogram) were enriched by flow cytometry. Open histogram: control SF767 parental cells. Inset: whole cell lysates of control SF767 cells or SF767 cells stably transduced with MAP4K4 shRNA (MAP4K4i) were immunoblotted with the indicated antibodies. (c) The migration rate of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), and MAP4K4i cells transfected with Pyk2 was assayed with a radial migration assay on 10 μ g/mL laminin substrate. * P

    Article Snippet: The coding sequence for full length MAP4K4 was isolated by RT-PCR of total RNA isolated from the SF767 glioma cell line using the Titan RT-PCR kit (Roche Applied Science, Indianapolis, IN) according to the manufacturer's instructions.

    Techniques: Expressing, Migration, Transfection, shRNA, Stable Transfection, Transduction, Flow Cytometry, Cytometry

    Schematic of MAP4K4 and interacting two-hybrid clones. (a) Structure of full length MAP4K4 indicating the N-terminal kinase domain, the C-terminal citron homology domain (CNH), and the location of alternatively spliced modules M1–M9. (b) Structure of the three overlapping clones isolated in the yeast two-hybrid genetic screen which interact with the Pyk2 FERM domain. (c) Structure of the final MAP4K4 clone assembled from SF767 glioma cell isoforms.

    Journal: Journal of Signal Transduction

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration

    doi: 10.1155/2013/956580

    Figure Lengend Snippet: Schematic of MAP4K4 and interacting two-hybrid clones. (a) Structure of full length MAP4K4 indicating the N-terminal kinase domain, the C-terminal citron homology domain (CNH), and the location of alternatively spliced modules M1–M9. (b) Structure of the three overlapping clones isolated in the yeast two-hybrid genetic screen which interact with the Pyk2 FERM domain. (c) Structure of the final MAP4K4 clone assembled from SF767 glioma cell isoforms.

    Article Snippet: The coding sequence for full length MAP4K4 was isolated by RT-PCR of total RNA isolated from the SF767 glioma cell line using the Titan RT-PCR kit (Roche Applied Science, Indianapolis, IN) according to the manufacturer's instructions.

    Techniques: Clone Assay, Isolation

    Pyk2 coimmunoprecipitates with MAP4K4. (a) Cells cotransfected with FLAG-epitope-tagged Pyk2 FERM and HA-epitope-tagged MAP4K4(143) were lysed and immunoprecipitated with anti-FLAG antibody or normal mouse IgG. Immunoprecipitates were immunoblotted (IB) with anti-FLAG or anti-HA. WCL: whole cell lysate. (b) Cells were cotransfected with FLAG-epitope-tagged MAP4K4(143) and either HA-tagged Pyk2 or HA-tagged FAK, lysed, and immunoprecipitated with mouse IgG or anti-FLAG antibody. Immunoprecipitates or WCL were immunoblotted as indicated. (c) Cells cotransfected with FLAG-tagged Pyk2 and either HA-tagged MAP4K4(143) or HA-tagged full length (FL) MAP4K4 were lysed and immunoprecipitated with rabbit IgG or anti-HA antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies. (d) SF767 glioma cells were lysed and immunoprecipitated with anti-MAP4K4 antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies.

    Journal: Journal of Signal Transduction

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration

    doi: 10.1155/2013/956580

    Figure Lengend Snippet: Pyk2 coimmunoprecipitates with MAP4K4. (a) Cells cotransfected with FLAG-epitope-tagged Pyk2 FERM and HA-epitope-tagged MAP4K4(143) were lysed and immunoprecipitated with anti-FLAG antibody or normal mouse IgG. Immunoprecipitates were immunoblotted (IB) with anti-FLAG or anti-HA. WCL: whole cell lysate. (b) Cells were cotransfected with FLAG-epitope-tagged MAP4K4(143) and either HA-tagged Pyk2 or HA-tagged FAK, lysed, and immunoprecipitated with mouse IgG or anti-FLAG antibody. Immunoprecipitates or WCL were immunoblotted as indicated. (c) Cells cotransfected with FLAG-tagged Pyk2 and either HA-tagged MAP4K4(143) or HA-tagged full length (FL) MAP4K4 were lysed and immunoprecipitated with rabbit IgG or anti-HA antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies. (d) SF767 glioma cells were lysed and immunoprecipitated with anti-MAP4K4 antibody. Immunoprecipitates or whole cell lysate (WCL) was immunoblotted with the indicated antibodies.

    Article Snippet: The coding sequence for full length MAP4K4 was isolated by RT-PCR of total RNA isolated from the SF767 glioma cell line using the Titan RT-PCR kit (Roche Applied Science, Indianapolis, IN) according to the manufacturer's instructions.

    Techniques: FLAG-tag, Immunoprecipitation

    Increased expression of MAP4K4 stimulates migration but is blocked by knockdown of Pyk2 expression. (a) Wild-type SF767 glioma cells (CTL) or SF767 cells stably transduced with a shRNA targeting Pyk2 (Pyk2i) were transfected with vector or HA-epitope-tagged MAP4K4. Whole cell lysates were blotted with the indicated antibodies. Lysates were blotted with actin as a loading control. (b) SF767 control cells or SF767 cells with shRNA mediated knockdown of Pyk2 (Pyk2i) were transfected with MAP4K4, and the migration rate on 10 μ g/mL laminin was assessed over 24 hr using a radial migration assay. † P = 0.0016 relative to control. * P

    Journal: Journal of Signal Transduction

    Article Title: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration

    doi: 10.1155/2013/956580

    Figure Lengend Snippet: Increased expression of MAP4K4 stimulates migration but is blocked by knockdown of Pyk2 expression. (a) Wild-type SF767 glioma cells (CTL) or SF767 cells stably transduced with a shRNA targeting Pyk2 (Pyk2i) were transfected with vector or HA-epitope-tagged MAP4K4. Whole cell lysates were blotted with the indicated antibodies. Lysates were blotted with actin as a loading control. (b) SF767 control cells or SF767 cells with shRNA mediated knockdown of Pyk2 (Pyk2i) were transfected with MAP4K4, and the migration rate on 10 μ g/mL laminin was assessed over 24 hr using a radial migration assay. † P = 0.0016 relative to control. * P

    Article Snippet: The coding sequence for full length MAP4K4 was isolated by RT-PCR of total RNA isolated from the SF767 glioma cell line using the Titan RT-PCR kit (Roche Applied Science, Indianapolis, IN) according to the manufacturer's instructions.

    Techniques: Expressing, Migration, CTL Assay, Stable Transfection, Transduction, shRNA, Transfection, Plasmid Preparation