serum specific ige detection  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    IgE Detection Antibody
    Description:

    Catalog Number:
    81-1016-01
    Price:
    None
    Buy from Supplier


    Structured Review

    Thermo Fisher serum specific ige detection

    https://www.bioz.com/result/serum specific ige detection/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    serum specific ige detection - by Bioz Stars, 2020-12
    99/100 stars

    Related Products / Commonly Used Together

    aeroallergens
    profilin alk
    panallergens ltp

    Images

    Related Articles

    Binding Assay:

    Article Title: Peptidomics of an in vitro digested α-Gal carrying protein revealed IgE-reactive peptides
    Article Snippet: .. Inhibition experiments The capacity of α-Gal peptides to inhibit IgE binding to solid phase-bound α-Gal was measured using the ImmunoCAP System (Thermo Fisher Scientific, Uppsala, Sweden). ..

    In Vitro:

    Article Title: Trajectories of childhood immune development and respiratory health relevant to asthma and allergy
    Article Snippet: .. These included markers of immune function: (1) IgG, IgG4, and IgE Phadiatop ImmunoCAP antibodies (ThermoFisher, Uppsala, Sweden), covering common allergens such as house-dust mite (HDM, Dermatophagoides pteronyssinus ), mould, couch grass, ryegrass, peanut, cat dander; (2) IgE and IgG4 Phadiatop Infant and Adult assays (ThermoFisher, Uppsala, Sweden) that target multiple allergens simultaneously ( ); (3) skin prick or sensitisation tests (SPT) for HDM, mould, ryegrass, cat, peanut, cow’s milk and hen’s egg; and (4) cytokine responses (IL-4,5,9,13,10, IFN-γ) following in vitro stimulation of extracted peripheral blood mononuclear cells (PBMCs) by multiple antigen and allergen stimuli, including phytohaemaglutinin (PHA), HDM, cat, peanut and ovalbumin ( ; ). .. In addition, nasopharyngeal samples (swabs or aspirates, NPAs) were taken from each child during healthy routine visits (healthy samples), and unscheduled visits where parents presented with their child if they have a suspected respiratory infection (disease samples).

    Single-particle Tracking:

    Article Title: Trajectories of childhood immune development and respiratory health relevant to asthma and allergy
    Article Snippet: .. These included markers of immune function: (1) IgG, IgG4, and IgE Phadiatop ImmunoCAP antibodies (ThermoFisher, Uppsala, Sweden), covering common allergens such as house-dust mite (HDM, Dermatophagoides pteronyssinus ), mould, couch grass, ryegrass, peanut, cat dander; (2) IgE and IgG4 Phadiatop Infant and Adult assays (ThermoFisher, Uppsala, Sweden) that target multiple allergens simultaneously ( ); (3) skin prick or sensitisation tests (SPT) for HDM, mould, ryegrass, cat, peanut, cow’s milk and hen’s egg; and (4) cytokine responses (IL-4,5,9,13,10, IFN-γ) following in vitro stimulation of extracted peripheral blood mononuclear cells (PBMCs) by multiple antigen and allergen stimuli, including phytohaemaglutinin (PHA), HDM, cat, peanut and ovalbumin ( ; ). .. In addition, nasopharyngeal samples (swabs or aspirates, NPAs) were taken from each child during healthy routine visits (healthy samples), and unscheduled visits where parents presented with their child if they have a suspected respiratory infection (disease samples).

    Incubation:

    Article Title: IgE actions on CD4+ T cells, mast cells, and macrophages participate in the pathogenesis of experimental abdominal aortic aneurysms
    Article Snippet: .. To localize IgE and FcεR1 to vascular SMCs, ECs, and CD4+ T cells, we performed double immunofluorescent staining (n = 11) with mouse anti-IgE (1:10) or rabbit anti-FcεR1 (1:80) antibodies incubated overnight, followed by anti-mouse or anti-rabbit Alexa-555 (Invitrogen, Grand Island, NY). .. Sections were washed, blocked with 4% of appropriate normal serum, and incubated with mouse anti-α-actin (1:40, HHF35; Enzo-Diagnostics, Syosset, NY), CD31 (1:40, Dako, Carpinteria, CA), and CD4 (1:20, Dako), followed by anti-mouse Alexa-488 (Invitrogen).

    Article Title: Reduced efficacy of treatment of strongyloidiasis in HTLV-I carriers related to enhanced expression of IFN-? and TGF-?1
    Article Snippet: .. Horseradish peroxidase (HRP)-conjugated mouse anti-human IgG1, IgG4 (Southern Biotechnology Associates, Birmingham, AL, USA) or HRP-conjugated goat anti-human IgA, IgE or IgG (Biosource International, Camarillo, CA, USA) was added to each well and the plates were incubated for 1 h at room temperature. .. After washing with 0·1% Tween 20 in PBS, substrate (Super Signal Substrate; Pierce) was added and the luminescence intensity was read with a microplate reader (Luminoskan; Labsystems).

    Article Title: Gender-Related Effects of Sex Steroids on Histamine Release and FcεRI Expression in Rat Peritoneal Mast Cells
    Article Snippet: .. For analysis of Fcε RI expression in the presence of IgE, PMCs were incubated for 16 h at 37°C with rat myeloma IgE (Invitrogen) at 5 μ g/mL, in addition to each steroid or culture media alone. .. After washing, cells were incubated with FITC-mouse anti-rat IgE antibody (Thermo Scientific).

    Article Title: Negative control of mast cell degranulation and the anaphylactic response by the phosphatase lipin1
    Article Snippet: .. BMMCs were sensitized with 1 μg/mL IgE for 6 h, resuspended at 1 × 107 cells/ml in Tyrode's buffer containing 3 μg/ml Indo-1 (Invitrogen) and 4 mM probencid, further incubated for 30 min at 37°C, and washed twice with Tyrode's buffer. .. 40 μl of cells was added into 460 μl of pre-warmed Tyrode's buffer to determine the calcium response by flow cytometry.

    Inhibition:

    Article Title: Peptidomics of an in vitro digested α-Gal carrying protein revealed IgE-reactive peptides
    Article Snippet: .. Inhibition experiments The capacity of α-Gal peptides to inhibit IgE binding to solid phase-bound α-Gal was measured using the ImmunoCAP System (Thermo Fisher Scientific, Uppsala, Sweden). ..

    Expressing:

    Article Title: Gender-Related Effects of Sex Steroids on Histamine Release and FcεRI Expression in Rat Peritoneal Mast Cells
    Article Snippet: .. For analysis of Fcε RI expression in the presence of IgE, PMCs were incubated for 16 h at 37°C with rat myeloma IgE (Invitrogen) at 5 μ g/mL, in addition to each steroid or culture media alone. .. After washing, cells were incubated with FITC-mouse anti-rat IgE antibody (Thermo Scientific).

    Staining:

    Article Title: IgE actions on CD4+ T cells, mast cells, and macrophages participate in the pathogenesis of experimental abdominal aortic aneurysms
    Article Snippet: .. To localize IgE and FcεR1 to vascular SMCs, ECs, and CD4+ T cells, we performed double immunofluorescent staining (n = 11) with mouse anti-IgE (1:10) or rabbit anti-FcεR1 (1:80) antibodies incubated overnight, followed by anti-mouse or anti-rabbit Alexa-555 (Invitrogen, Grand Island, NY). .. Sections were washed, blocked with 4% of appropriate normal serum, and incubated with mouse anti-α-actin (1:40, HHF35; Enzo-Diagnostics, Syosset, NY), CD31 (1:40, Dako, Carpinteria, CA), and CD4 (1:20, Dako), followed by anti-mouse Alexa-488 (Invitrogen).

    Recombinant:

    Article Title: Mast cell activation test in the diagnosis of allergic disease and anaphylaxis
    Article Snippet: .. Specific IgE to whole allergen extract, component-resolved diagnostics, and SPTs Levels of total IgE, peanut-specific IgE, and IgE to the recombinant allergen components rAra h 1, 2, 3, 6, 8, and 9 were measured by using ImmunoCAP (Thermo Fisher Scientific, Uppsala, Sweden). .. SPTs were undertaken according to national guidelines by using lancets (ALK-Abelló, Hørsholm, Denmark) and commercial peanut extract (Stallergenes, Paris, France), with 1% histamine as a positive control.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Thermo Fisher ova ige absorbance values
    Effects of casticin (CAS) on <t>OVA-specific</t> antibodies in serum. Serum levels of <t>OVA-IgE</t> and OVA-IgG1 in normal (N) and OVA-stimulated (OVA) mice with or without CAS and prednisolone (P) treatment. CAS also changed the levels of IL-4, IL-5, and IL-13 produced by OVA-activated splenocytes. The data are presented as means ± SEM of three independent experiments ( n = 12). ∗ p
    Ova Ige Absorbance Values, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ova ige absorbance values/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ova ige absorbance values - by Bioz Stars, 2020-12
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effects of casticin (CAS) on OVA-specific antibodies in serum. Serum levels of OVA-IgE and OVA-IgG1 in normal (N) and OVA-stimulated (OVA) mice with or without CAS and prednisolone (P) treatment. CAS also changed the levels of IL-4, IL-5, and IL-13 produced by OVA-activated splenocytes. The data are presented as means ± SEM of three independent experiments ( n = 12). ∗ p

    Journal: Frontiers in Pharmacology

    Article Title: Protective Effects of Casticin From Vitex trifolia Alleviate Eosinophilic Airway Inflammation and Oxidative Stress in a Murine Asthma Model

    doi: 10.3389/fphar.2018.00635

    Figure Lengend Snippet: Effects of casticin (CAS) on OVA-specific antibodies in serum. Serum levels of OVA-IgE and OVA-IgG1 in normal (N) and OVA-stimulated (OVA) mice with or without CAS and prednisolone (P) treatment. CAS also changed the levels of IL-4, IL-5, and IL-13 produced by OVA-activated splenocytes. The data are presented as means ± SEM of three independent experiments ( n = 12). ∗ p

    Article Snippet: Serum was diluted fivefold to measure the OVA-IgE absorbance values as the optical density at 450 nm using a microplate reader (Thermo).

    Techniques: Mouse Assay, Produced