serum sige levels  (Thermo Fisher)


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    Structured Review

    Thermo Fisher serum sige levels
    ROC curves for determining immediate hypersensitivity to <t>cefaclor</t> (A) and discriminating anaphylaxis among patiens with cefaclor hypersensitivity (B). <t>sIgE,</t> specific IgE; CI, confidence interval; AUC, area under the curve; ROC, receiver operating characteristic.
    Serum Sige Levels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Proper Cut-off Levels of Serum Specific IgE to Cefaclor for Patients with Cefaclor Allergy"

    Article Title: Proper Cut-off Levels of Serum Specific IgE to Cefaclor for Patients with Cefaclor Allergy

    Journal: Yonsei Medical Journal

    doi: 10.3349/ymj.2018.59.8.968

    ROC curves for determining immediate hypersensitivity to cefaclor (A) and discriminating anaphylaxis among patiens with cefaclor hypersensitivity (B). sIgE, specific IgE; CI, confidence interval; AUC, area under the curve; ROC, receiver operating characteristic.
    Figure Legend Snippet: ROC curves for determining immediate hypersensitivity to cefaclor (A) and discriminating anaphylaxis among patiens with cefaclor hypersensitivity (B). sIgE, specific IgE; CI, confidence interval; AUC, area under the curve; ROC, receiver operating characteristic.

    Techniques Used:

    Levels of serum sIgE to cefaclor according to clinical phenotypes of cefaclor hypersensitivity. sIgE, specific IgE.
    Figure Legend Snippet: Levels of serum sIgE to cefaclor according to clinical phenotypes of cefaclor hypersensitivity. sIgE, specific IgE.

    Techniques Used:

    2) Product Images from "Dissociating polysensitization and multimorbidity in children and adults from a Polish general population cohort"

    Article Title: Dissociating polysensitization and multimorbidity in children and adults from a Polish general population cohort

    Journal: Clinical and Translational Allergy

    doi: 10.1186/s13601-019-0246-y

    Percentage of people with allergic multimorbidity by the number of positive allergen-specific IgE assays (4 allergens: d1, e1, m6, g6). X axis—sIgE assay; Y axis—% of multimorbidity among those with positive sIgE for 0, 1, 2 and 3 allergens
    Figure Legend Snippet: Percentage of people with allergic multimorbidity by the number of positive allergen-specific IgE assays (4 allergens: d1, e1, m6, g6). X axis—sIgE assay; Y axis—% of multimorbidity among those with positive sIgE for 0, 1, 2 and 3 allergens

    Techniques Used:

    3) Product Images from "Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation"

    Article Title: Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02718

    Hypothetical model of the involvement of IL-6 from different cellular sources in the pathogenesis of HDM-induced allergic asthma. We propose that IL-6 produced by macrophages and dendritic cells distinctively promotes HDM-induced airway inflammation. IL-6 produced by macrophages contributes to type 2 allergic inflammation, including eosinophil accumulation, IgE production and mucus hypersecretion via induction of Th2 cells differentiation and production of IL-33, TSLP, IL-4, IL-13 cytokines. In contrast, IL-6 from dendritic cells induces pathogenic accumulation of neutrophils via induction of IL-17A and IFNγ, produced by Th17 and Th1 subsets. Therefore, selective targeting of IL-6 in macrophages or dendritic cells can be beneficial in corresponding eosinophilic and neutrophilic asthma endotypes.
    Figure Legend Snippet: Hypothetical model of the involvement of IL-6 from different cellular sources in the pathogenesis of HDM-induced allergic asthma. We propose that IL-6 produced by macrophages and dendritic cells distinctively promotes HDM-induced airway inflammation. IL-6 produced by macrophages contributes to type 2 allergic inflammation, including eosinophil accumulation, IgE production and mucus hypersecretion via induction of Th2 cells differentiation and production of IL-33, TSLP, IL-4, IL-13 cytokines. In contrast, IL-6 from dendritic cells induces pathogenic accumulation of neutrophils via induction of IL-17A and IFNγ, produced by Th17 and Th1 subsets. Therefore, selective targeting of IL-6 in macrophages or dendritic cells can be beneficial in corresponding eosinophilic and neutrophilic asthma endotypes.

    Techniques Used: Produced

    4) Product Images from "Clinical Characteristics of Oral Allergy Syndrome in Children with Atopic Dermatitis and Birch Sensitization: a Single Center Study"

    Article Title: Clinical Characteristics of Oral Allergy Syndrome in Children with Atopic Dermatitis and Birch Sensitization: a Single Center Study

    Journal: Journal of Korean Medical Science

    doi: 10.3346/jkms.2019.34.e11

    Optimal cut-off values of birch-sIgE established via ROC analysis. sIgE = specific immunoglobulin E, ROC = receiver operating characteristic, AUC = area under the curve, CI = confidence interval, PPV = positive predictive value, NPV = negative predictive value.
    Figure Legend Snippet: Optimal cut-off values of birch-sIgE established via ROC analysis. sIgE = specific immunoglobulin E, ROC = receiver operating characteristic, AUC = area under the curve, CI = confidence interval, PPV = positive predictive value, NPV = negative predictive value.

    Techniques Used:

    5) Product Images from "Clinical Characteristics of Oral Allergy Syndrome in Children with Atopic Dermatitis and Birch Sensitization: a Single Center Study"

    Article Title: Clinical Characteristics of Oral Allergy Syndrome in Children with Atopic Dermatitis and Birch Sensitization: a Single Center Study

    Journal: Journal of Korean Medical Science

    doi: 10.3346/jkms.2019.34.e11

    Optimal cut-off values of birch-sIgE established via ROC analysis. sIgE = specific immunoglobulin E, ROC = receiver operating characteristic, AUC = area under the curve, CI = confidence interval, PPV = positive predictive value, NPV = negative predictive value.
    Figure Legend Snippet: Optimal cut-off values of birch-sIgE established via ROC analysis. sIgE = specific immunoglobulin E, ROC = receiver operating characteristic, AUC = area under the curve, CI = confidence interval, PPV = positive predictive value, NPV = negative predictive value.

    Techniques Used:

    6) Product Images from "The parasitic worm-derived immunomodulator, ES-62 and its drug-like small molecule analogues exhibit therapeutic potential in a model of chronic asthma"

    Article Title: The parasitic worm-derived immunomodulator, ES-62 and its drug-like small molecule analogues exhibit therapeutic potential in a model of chronic asthma

    Journal: Scientific Reports

    doi: 10.1038/srep19224

    Inflammatory parameters of the C57BL/6 mouse model of chronic asthma. ( a ) IL-4 and IL-13 in the BALF are presented as mean ± SEM values of the mean levels with respect to individual mice (n = 5) at each time point; ( b ) IL-17E (IL-25; n = 5), IL-17F (n = 5) and IL-22 (n = 3) in the BALF are presented as mean ± SEM values of the mean levels in the indicated numbers of individual mice at each time point; ( c ) MCP-1 (n = 5), exotaxin-1 (n = 5–8) and eotaxin-2 (n = 5–8) in the BALF are presented as mean ± SEM values of the mean levels in the indicated numbers of individual mice at each time point; ( d ) Periostin in the BALF is presented as mean ± SEM values of the mean levels in individual mice (n = 3–5) at each time point; ( e ) OVA-specific IgE in the BALF is presented as mean ± SEM values of the mean OD450 values for individual mice (n = 3–4) at each time point whilst the levels of plasma cells (PC) in pooled samples at each time point are presented as the % of live BALF cells; ( f ) OVA-specific IgE, IgG1 and IgG2a levels in serum are presented as mean ± SEM values of the mean OD450 values for individual mice (n = 5) at each time point; levels of splenic germinal centre (GC) B cells and PC ( g ) and IL-10 + Breg and Tr1 cells ( h ) presented as mean % ± SEM values of individual mice (n = 5) at each time point.
    Figure Legend Snippet: Inflammatory parameters of the C57BL/6 mouse model of chronic asthma. ( a ) IL-4 and IL-13 in the BALF are presented as mean ± SEM values of the mean levels with respect to individual mice (n = 5) at each time point; ( b ) IL-17E (IL-25; n = 5), IL-17F (n = 5) and IL-22 (n = 3) in the BALF are presented as mean ± SEM values of the mean levels in the indicated numbers of individual mice at each time point; ( c ) MCP-1 (n = 5), exotaxin-1 (n = 5–8) and eotaxin-2 (n = 5–8) in the BALF are presented as mean ± SEM values of the mean levels in the indicated numbers of individual mice at each time point; ( d ) Periostin in the BALF is presented as mean ± SEM values of the mean levels in individual mice (n = 3–5) at each time point; ( e ) OVA-specific IgE in the BALF is presented as mean ± SEM values of the mean OD450 values for individual mice (n = 3–4) at each time point whilst the levels of plasma cells (PC) in pooled samples at each time point are presented as the % of live BALF cells; ( f ) OVA-specific IgE, IgG1 and IgG2a levels in serum are presented as mean ± SEM values of the mean OD450 values for individual mice (n = 5) at each time point; levels of splenic germinal centre (GC) B cells and PC ( g ) and IL-10 + Breg and Tr1 cells ( h ) presented as mean % ± SEM values of individual mice (n = 5) at each time point.

    Techniques Used: Mouse Assay

    7) Product Images from "Protective Effects of Casticin From Vitex trifolia Alleviate Eosinophilic Airway Inflammation and Oxidative Stress in a Murine Asthma Model"

    Article Title: Protective Effects of Casticin From Vitex trifolia Alleviate Eosinophilic Airway Inflammation and Oxidative Stress in a Murine Asthma Model

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.00635

    Effects of casticin (CAS) on OVA-specific antibodies in serum. Serum levels of OVA-IgE and OVA-IgG1 in normal (N) and OVA-stimulated (OVA) mice with or without CAS and prednisolone (P) treatment. CAS also changed the levels of IL-4, IL-5, and IL-13 produced by OVA-activated splenocytes. The data are presented as means ± SEM of three independent experiments ( n = 12). ∗ p
    Figure Legend Snippet: Effects of casticin (CAS) on OVA-specific antibodies in serum. Serum levels of OVA-IgE and OVA-IgG1 in normal (N) and OVA-stimulated (OVA) mice with or without CAS and prednisolone (P) treatment. CAS also changed the levels of IL-4, IL-5, and IL-13 produced by OVA-activated splenocytes. The data are presented as means ± SEM of three independent experiments ( n = 12). ∗ p

    Techniques Used: Mouse Assay, Produced

    8) Product Images from "Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients"

    Article Title: Studies of royal jelly and associated cross-reactive allergens in atopic dermatitis patients

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0233707

    Correlations between anti-RJ antibody titers and clinical findings in the AD patients who participated in the study. The binary logarithm of the antibody titer was plotted, and negative results are represented as zero for convenience. Significant correlations were observed for nonspecific IgE (A) and the EASI score (B) by Spearman’s rank correlation test. rs represents Spearman’s rho (correlation coefficient).
    Figure Legend Snippet: Correlations between anti-RJ antibody titers and clinical findings in the AD patients who participated in the study. The binary logarithm of the antibody titer was plotted, and negative results are represented as zero for convenience. Significant correlations were observed for nonspecific IgE (A) and the EASI score (B) by Spearman’s rank correlation test. rs represents Spearman’s rho (correlation coefficient).

    Techniques Used:

    9) Product Images from "Quail egg homogenate alleviates food allergy induced eosinophilic esophagitis like disease through modulating PAR-2 transduction pathway in peanut sensitized mice"

    Article Title: Quail egg homogenate alleviates food allergy induced eosinophilic esophagitis like disease through modulating PAR-2 transduction pathway in peanut sensitized mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-19309-x

    Oral quail egg treatment reduced PPE specific IgE, IgG1, and the level release of allergic mediators (n = 10). ( A ) PPE and quail egg specific IgE levels ( B ) PPE and quail egg specific IgG1 levels; Allergic mediators: ( C ) histamine, ( D ) tryptase; ( E ) ECP. Results are expressed as mean ± SEM. * P
    Figure Legend Snippet: Oral quail egg treatment reduced PPE specific IgE, IgG1, and the level release of allergic mediators (n = 10). ( A ) PPE and quail egg specific IgE levels ( B ) PPE and quail egg specific IgG1 levels; Allergic mediators: ( C ) histamine, ( D ) tryptase; ( E ) ECP. Results are expressed as mean ± SEM. * P

    Techniques Used:

    10) Product Images from "Oral Food Desensitization in Children With IgE-Mediated Cow's Milk Allergy: Immunological Changes Underlying Desensitization"

    Article Title: Oral Food Desensitization in Children With IgE-Mediated Cow's Milk Allergy: Immunological Changes Underlying Desensitization

    Journal: Allergy, Asthma & Immunology Research

    doi: 10.4168/aair.2017.9.1.35

    Antibody response in CM-allergic patients. Serum-specific (A) IgE (kU/L) and (B) IgG4 (µg/L) to CM, α-La, β-Lg, and CN before (Orange blocks) and after (Blue blocks) the OIT protocol in the CM-allergic patients who tolerated at least 200 mL of cow's milk (n=14). Bars represent mean±SEM. *** P
    Figure Legend Snippet: Antibody response in CM-allergic patients. Serum-specific (A) IgE (kU/L) and (B) IgG4 (µg/L) to CM, α-La, β-Lg, and CN before (Orange blocks) and after (Blue blocks) the OIT protocol in the CM-allergic patients who tolerated at least 200 mL of cow's milk (n=14). Bars represent mean±SEM. *** P

    Techniques Used:

    11) Product Images from "Gut microbiota components are associated with fixed airway obstruction in asthmatic patients living in the tropics"

    Article Title: Gut microbiota components are associated with fixed airway obstruction in asthmatic patients living in the tropics

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-27964-3

    Partial least squares discriminant analysis of gut microbiome composition between low and high IgE responders to B. tropicalis . sPLS-DA plot based on the relative abundance of bacterial taxa of gut microbiota from low (blue circle, percentile 1) and high IgE responders (orange triangle, percentile 4) and their 95% confidence ellipses ( A ). Contribution plot indicating genera contributing to component 1 of the sPLS-DA plot that discriminate first and fourth sIgE percentiles ( B ). The abundance of the most consistent OTUs was compared using Metagenomeseq and presented on a violin plot, which includes the median, 95% CI, IQR, and density plot where the width of the blue lines indicate frequency ( C ).
    Figure Legend Snippet: Partial least squares discriminant analysis of gut microbiome composition between low and high IgE responders to B. tropicalis . sPLS-DA plot based on the relative abundance of bacterial taxa of gut microbiota from low (blue circle, percentile 1) and high IgE responders (orange triangle, percentile 4) and their 95% confidence ellipses ( A ). Contribution plot indicating genera contributing to component 1 of the sPLS-DA plot that discriminate first and fourth sIgE percentiles ( B ). The abundance of the most consistent OTUs was compared using Metagenomeseq and presented on a violin plot, which includes the median, 95% CI, IQR, and density plot where the width of the blue lines indicate frequency ( C ).

    Techniques Used:

    12) Product Images from "March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation"

    Article Title: March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation

    Journal: Journal of Immunology Research

    doi: 10.1155/2018/3823910

    March1 deficiency leads to less antibody production against OVA in the ovalbumin-allergic model. Total IgE (a), OVA-specific IgE (b), and OVA-specific IgG 1 (c) were measured in the serum of WT and March1 −/− mice. ∗ p
    Figure Legend Snippet: March1 deficiency leads to less antibody production against OVA in the ovalbumin-allergic model. Total IgE (a), OVA-specific IgE (b), and OVA-specific IgG 1 (c) were measured in the serum of WT and March1 −/− mice. ∗ p

    Techniques Used: Mouse Assay

    13) Product Images from "Tolerogenic Dendritic Cells Derived from Donors with Natural Rubber Latex Allergy Modulate Allergen-Specific T-Cell Responses and IgE Production"

    Article Title: Tolerogenic Dendritic Cells Derived from Donors with Natural Rubber Latex Allergy Modulate Allergen-Specific T-Cell Responses and IgE Production

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0085930

    Dexamethasone-treated dendritic cells (dxDC) decrease total and allergen-specific IgE production by human autologous naïve B cells from natural rubber latex (NRL)-allergic patients. Naïve T cells and B cells were stimulated with Hev b 5-pulsed mDC or dxDC in the presence of CD40L + 3T3 fibroblasts and IL-4 for 10 days. B cells stimulated in presence of IL-4 and CD40L were used as positive control. Total IgE (A) and Hev b 5-specific IgE (B) production was measured by ELISA. Each group of bars represents the mean+SD of experiments performed in duplicate on 3 different donors (NLR 1, 5 and 7). * p
    Figure Legend Snippet: Dexamethasone-treated dendritic cells (dxDC) decrease total and allergen-specific IgE production by human autologous naïve B cells from natural rubber latex (NRL)-allergic patients. Naïve T cells and B cells were stimulated with Hev b 5-pulsed mDC or dxDC in the presence of CD40L + 3T3 fibroblasts and IL-4 for 10 days. B cells stimulated in presence of IL-4 and CD40L were used as positive control. Total IgE (A) and Hev b 5-specific IgE (B) production was measured by ELISA. Each group of bars represents the mean+SD of experiments performed in duplicate on 3 different donors (NLR 1, 5 and 7). * p

    Techniques Used: Positive Control, Enzyme-linked Immunosorbent Assay

    Dexamethasone-treated dendritic cells (dxDC) from natural rubber latex (NRL)-allergic patients display an anti-inflammatory cytokine profile. IL-12 and IL-10 production was analyzed by ELISPOT after DC were activated with CD40L overnight. Results are the mean+SD of 5 different patients performed in duplicate. *** p
    Figure Legend Snippet: Dexamethasone-treated dendritic cells (dxDC) from natural rubber latex (NRL)-allergic patients display an anti-inflammatory cytokine profile. IL-12 and IL-10 production was analyzed by ELISPOT after DC were activated with CD40L overnight. Results are the mean+SD of 5 different patients performed in duplicate. *** p

    Techniques Used: Enzyme-linked Immunospot

    14) Product Images from "LUZ-Y, a Novel Platform for the Mammalian Cell Production of Full-length IgG-bispecific Antibodies *"

    Article Title: LUZ-Y, a Novel Platform for the Mammalian Cell Production of Full-length IgG-bispecific Antibodies *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.397869

    Biological activity of LUZ-Y. a , effect of anti-hFcϵRIα/hFcγRIIb-bispecific antibodies on histamine release from IgE-activated rat basophil leukemia cells expressing hFcϵRIα and hFcγRIIb. Common light chain LUZ-Y ( blue ), knobs-into-holes antibody ( red ), no treatment ( purple ). Duplicate samples of each antibody were assayed, and data are shown for each of the duplicates. b , effect of LUZ-Y antibodies on the proliferation of FaDu cells. FaDu cells were incubated, in duplicate, with the indicated concentrations of the parental anti-EGFR and HER3 antibodies separately and in combination, or with the anti-EGFR/HER3 LUZ-Y in 1% serum containing media. Cell proliferation was analyzed after three days by AlamarBlue staining.
    Figure Legend Snippet: Biological activity of LUZ-Y. a , effect of anti-hFcϵRIα/hFcγRIIb-bispecific antibodies on histamine release from IgE-activated rat basophil leukemia cells expressing hFcϵRIα and hFcγRIIb. Common light chain LUZ-Y ( blue ), knobs-into-holes antibody ( red ), no treatment ( purple ). Duplicate samples of each antibody were assayed, and data are shown for each of the duplicates. b , effect of LUZ-Y antibodies on the proliferation of FaDu cells. FaDu cells were incubated, in duplicate, with the indicated concentrations of the parental anti-EGFR and HER3 antibodies separately and in combination, or with the anti-EGFR/HER3 LUZ-Y in 1% serum containing media. Cell proliferation was analyzed after three days by AlamarBlue staining.

    Techniques Used: Activity Assay, Expressing, Incubation, Staining

    15) Product Images from "Gut microbiota components are associated with fixed airway obstruction in asthmatic patients living in the tropics"

    Article Title: Gut microbiota components are associated with fixed airway obstruction in asthmatic patients living in the tropics

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-27964-3

    Partial least squares discriminant analysis of gut microbiome composition between low and high IgE responders to B. tropicalis . sPLS-DA plot based on the relative abundance of bacterial taxa of gut microbiota from low (blue circle, percentile 1) and high IgE responders (orange triangle, percentile 4) and their 95% confidence ellipses ( A ). Contribution plot indicating genera contributing to component 1 of the sPLS-DA plot that discriminate first and fourth sIgE percentiles ( B ). The abundance of the most consistent OTUs was compared using Metagenomeseq and presented on a violin plot, which includes the median, 95% CI, IQR, and density plot where the width of the blue lines indicate frequency ( C ).
    Figure Legend Snippet: Partial least squares discriminant analysis of gut microbiome composition between low and high IgE responders to B. tropicalis . sPLS-DA plot based on the relative abundance of bacterial taxa of gut microbiota from low (blue circle, percentile 1) and high IgE responders (orange triangle, percentile 4) and their 95% confidence ellipses ( A ). Contribution plot indicating genera contributing to component 1 of the sPLS-DA plot that discriminate first and fourth sIgE percentiles ( B ). The abundance of the most consistent OTUs was compared using Metagenomeseq and presented on a violin plot, which includes the median, 95% CI, IQR, and density plot where the width of the blue lines indicate frequency ( C ).

    Techniques Used:

    16) Product Images from "Proper Cut-off Levels of Serum Specific IgE to Cefaclor for Patients with Cefaclor Allergy"

    Article Title: Proper Cut-off Levels of Serum Specific IgE to Cefaclor for Patients with Cefaclor Allergy

    Journal: Yonsei Medical Journal

    doi: 10.3349/ymj.2018.59.8.968

    Levels of serum sIgE to cefaclor according to clinical phenotypes of cefaclor hypersensitivity. sIgE, specific IgE.
    Figure Legend Snippet: Levels of serum sIgE to cefaclor according to clinical phenotypes of cefaclor hypersensitivity. sIgE, specific IgE.

    Techniques Used:

    17) Product Images from "Basophil Activation Test Using Recombinant Allergens: Highly Specific Diagnostic Method Complementing Routine Tests in Wasp Venom Allergy"

    Article Title: Basophil Activation Test Using Recombinant Allergens: Highly Specific Diagnostic Method Complementing Routine Tests in Wasp Venom Allergy

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0108619

    Recombinant allergens improve the in vitro test specificity for the identification of wasp venom allergic patients. ROC curves of specific IgE measurement (sIgE) of recombinant Ves v 1, Ves v 2 and Ves v 3 by ELISA (O.D.) ( A–C ) and recombinant Ves v 5 by ImmunoCAP (KU/L) ( D ). ROC curves of CD63% trade-off of basophil stimulated with recombinant Ves v 1, Ves v 2, Ves v 3 and Ves v 5 ( E–H ).
    Figure Legend Snippet: Recombinant allergens improve the in vitro test specificity for the identification of wasp venom allergic patients. ROC curves of specific IgE measurement (sIgE) of recombinant Ves v 1, Ves v 2 and Ves v 3 by ELISA (O.D.) ( A–C ) and recombinant Ves v 5 by ImmunoCAP (KU/L) ( D ). ROC curves of CD63% trade-off of basophil stimulated with recombinant Ves v 1, Ves v 2, Ves v 3 and Ves v 5 ( E–H ).

    Techniques Used: Recombinant, In Vitro, Enzyme-linked Immunosorbent Assay

    18) Product Images from "A Solid-in-Oil Nanodispersion System for Transcutaneous Immunotherapy of Cow’s Milk Allergies"

    Article Title: A Solid-in-Oil Nanodispersion System for Transcutaneous Immunotherapy of Cow’s Milk Allergies

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics12030205

    Serum antibody responses after antigen immunotherapy. ( a ) Schematic of the transcutaneous immunotherapy (TCIT) schedule. Mice were treated with a patch containing S/O nanoparticles carrying BLG without (S/O patch) or with (R848 S/O patch) R-848 in an IPM vehicle or a PBS-based solution of BLG (PBS sol. patch) or were subcutaneously injected with a PBS-based solution of BLG (PBS sol. s.c. inj). ( b , c ) The levels of total IgE ( b ) and BLG-specific IgG1 and IgG2a ( c ) in sera from mice treated as described in ( a ) were determined by enzyme-linked immunosorbent assay (ELISA). BLG dissolved in PBS (PBS sol.) was administered as a negative control. Data are shown as the mean + SD ( n = 6; no significant differences were observed).
    Figure Legend Snippet: Serum antibody responses after antigen immunotherapy. ( a ) Schematic of the transcutaneous immunotherapy (TCIT) schedule. Mice were treated with a patch containing S/O nanoparticles carrying BLG without (S/O patch) or with (R848 S/O patch) R-848 in an IPM vehicle or a PBS-based solution of BLG (PBS sol. patch) or were subcutaneously injected with a PBS-based solution of BLG (PBS sol. s.c. inj). ( b , c ) The levels of total IgE ( b ) and BLG-specific IgG1 and IgG2a ( c ) in sera from mice treated as described in ( a ) were determined by enzyme-linked immunosorbent assay (ELISA). BLG dissolved in PBS (PBS sol.) was administered as a negative control. Data are shown as the mean + SD ( n = 6; no significant differences were observed).

    Techniques Used: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Negative Control

    19) Product Images from "Specific immunotherapy ameliorates ulcerative colitis"

    Article Title: Specific immunotherapy ameliorates ulcerative colitis

    Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

    doi: 10.1186/s13223-016-0142-0

    Effect of the therapy on serum IgE, IgG4 and CD4 + T cell cytokines. Peripheral blood samples were collected from each patient before and after the therapy. The sera were analyzed by ELISA. The bars indicate the serum levels of SIgE ( a ), SIgG4 ( b ), IL-4 ( c ), IFN-g ( d ), IL-17 ( e ) and TNF-alpha ( f ). The data are presented as mean ± SD. *p
    Figure Legend Snippet: Effect of the therapy on serum IgE, IgG4 and CD4 + T cell cytokines. Peripheral blood samples were collected from each patient before and after the therapy. The sera were analyzed by ELISA. The bars indicate the serum levels of SIgE ( a ), SIgG4 ( b ), IL-4 ( c ), IFN-g ( d ), IL-17 ( e ) and TNF-alpha ( f ). The data are presented as mean ± SD. *p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    20) Product Images from "Mechanisms, safety and efficacy of a B cell epitope-based vaccine for immunotherapy of grass pollen allergy"

    Article Title: Mechanisms, safety and efficacy of a B cell epitope-based vaccine for immunotherapy of grass pollen allergy

    Journal: EBioMedicine

    doi: 10.1016/j.ebiom.2016.08.022

    Quantification of grass pollen allergen-specific IgE levels in the treatment groups before and after vaccination. Timothy grass pollen allergen extract-specific IgE levels determined by ImmunoCAP (y-axes: kUA/l, horizontal bars denote medians) in sera from the four patient groups (S1: 10 μg; S2: 20 μg; S3: 40 μg; placebo) before (pre) and after (post) treatment.
    Figure Legend Snippet: Quantification of grass pollen allergen-specific IgE levels in the treatment groups before and after vaccination. Timothy grass pollen allergen extract-specific IgE levels determined by ImmunoCAP (y-axes: kUA/l, horizontal bars denote medians) in sera from the four patient groups (S1: 10 μg; S2: 20 μg; S3: 40 μg; placebo) before (pre) and after (post) treatment.

    Techniques Used:

    21) Product Images from "In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system"

    Article Title: In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-8-41

    Purification of biotinylated scFv from culture supernatant . Western blot analysis of biotinylated scFv P3 purified in a low affinity avidin column and eluted with biotin, detected with anti-SV5 tag mAb (A) or with HRP-conjugated streptavidin (B). The eluted molecules (more than 90% of input) were about 10-fold more concentrated than in the supernatant.
    Figure Legend Snippet: Purification of biotinylated scFv from culture supernatant . Western blot analysis of biotinylated scFv P3 purified in a low affinity avidin column and eluted with biotin, detected with anti-SV5 tag mAb (A) or with HRP-conjugated streptavidin (B). The eluted molecules (more than 90% of input) were about 10-fold more concentrated than in the supernatant.

    Techniques Used: Purification, Western Blot, Avidin-Biotin Assay

    Biotinylation of secretory scFv proteins . Western blot gel retardation assay of supernatants from cells transiently transfected (A) or stably transfected (B) with the bigenic vector expressing the indicated scFv. (C) Supernatants from stably transfected cells taken at different culture times. Where indicated, cell cultures were supplemented with biotin for 24 h and dialyzed samples reacted with streptavidin. (D) ELISA of biotinylated or non-biotinylated scFv P3 (coating chimeric mAb 1E10) and scFv 1E10 (coating chimeric mAb P3); binding was revealed with anti-SV5 tag mAb or HRP-conjugated-streptavidin. (E) Flow cytometry and (F) immunofluorescence microscopy of biotinylated scFv s on cells expressing specific cell-surface ligands. scFv P3 (left panels) was reacted with cells displaying idiotype 1E10; scFv 9E1 (right panels) was reacted with RBL-SX38 expressing human FcεRI. Binding was performed with biotinylated (red) or non-biotinylated (violet) scFv s and with Quantum Dots-conjugated streptavidin. The green signal corresponds to FITC-conjugated anti-human IgE (ε-chain specific) antibody that recognizes the ε-membrane SIP displaying the idiotype.
    Figure Legend Snippet: Biotinylation of secretory scFv proteins . Western blot gel retardation assay of supernatants from cells transiently transfected (A) or stably transfected (B) with the bigenic vector expressing the indicated scFv. (C) Supernatants from stably transfected cells taken at different culture times. Where indicated, cell cultures were supplemented with biotin for 24 h and dialyzed samples reacted with streptavidin. (D) ELISA of biotinylated or non-biotinylated scFv P3 (coating chimeric mAb 1E10) and scFv 1E10 (coating chimeric mAb P3); binding was revealed with anti-SV5 tag mAb or HRP-conjugated-streptavidin. (E) Flow cytometry and (F) immunofluorescence microscopy of biotinylated scFv s on cells expressing specific cell-surface ligands. scFv P3 (left panels) was reacted with cells displaying idiotype 1E10; scFv 9E1 (right panels) was reacted with RBL-SX38 expressing human FcεRI. Binding was performed with biotinylated (red) or non-biotinylated (violet) scFv s and with Quantum Dots-conjugated streptavidin. The green signal corresponds to FITC-conjugated anti-human IgE (ε-chain specific) antibody that recognizes the ε-membrane SIP displaying the idiotype.

    Techniques Used: Western Blot, Electrophoretic Mobility Shift Assay, Transfection, Stable Transfection, Plasmid Preparation, Expressing, Enzyme-linked Immunosorbent Assay, Binding Assay, Flow Cytometry, Cytometry, Immunofluorescence, Microscopy

    Biotinylation efficiency of secretory and membrane bound t-IgE . (A) Western blot gel retardation assay of supernatants (t-sIgE) or cell extracts (t-m L IgE) from transiently transfected cells expressing secretory or membrane bound t-IgE. Where indicated, cell cultures were supplemented with biotin for 24 h and supernatants (dialyzed) or cell extracts reacted with streptavidin. (B) Flow cytometry and immunofluorescence microscopy assay on RBL-SX38 cells expressing human FcεRI incubated with biotinylated (red) or non-biotinylated (violet) t-sIgE; mAb 9E1 was detected with FITC-conjugated anti-mouse IgG antibody. (C) Flow cytometry and immunofluorescence microscopy assay of t-m L IgE displayed on transiently transfected HEK293T/17 cells, cultured in the presence (red) or absence (green) of biotin.
    Figure Legend Snippet: Biotinylation efficiency of secretory and membrane bound t-IgE . (A) Western blot gel retardation assay of supernatants (t-sIgE) or cell extracts (t-m L IgE) from transiently transfected cells expressing secretory or membrane bound t-IgE. Where indicated, cell cultures were supplemented with biotin for 24 h and supernatants (dialyzed) or cell extracts reacted with streptavidin. (B) Flow cytometry and immunofluorescence microscopy assay on RBL-SX38 cells expressing human FcεRI incubated with biotinylated (red) or non-biotinylated (violet) t-sIgE; mAb 9E1 was detected with FITC-conjugated anti-mouse IgG antibody. (C) Flow cytometry and immunofluorescence microscopy assay of t-m L IgE displayed on transiently transfected HEK293T/17 cells, cultured in the presence (red) or absence (green) of biotin.

    Techniques Used: Western Blot, Electrophoretic Mobility Shift Assay, Transfection, Expressing, Flow Cytometry, Cytometry, Immunofluorescence, Microscopy, Incubation, Cell Culture

    Biotinylation of the soluble high affinity receptor FcεRI α-chain . A) Western blot gel retardation assay of culture supernatants from cells expressing αD1D2. Where indicated, cell cultures were supplemented with biotin for 24 h and dialyzed samples reacted with streptavidin. (B) ELISA on IgE-coated plates of αD1D2, revealed with anti-SV5 tag mAb or HRP-conjugated streptavidin. (C) Flow cytometry and immunofluorescence microscopy assay with biotinylated (red) or non-biotinylated (violet) αD1D2 on transfected A20 cells expressing human membrane IgE; the green signal corresponds to FITC-conjugated anti-human IgE (ε-chain specific) antibody.
    Figure Legend Snippet: Biotinylation of the soluble high affinity receptor FcεRI α-chain . A) Western blot gel retardation assay of culture supernatants from cells expressing αD1D2. Where indicated, cell cultures were supplemented with biotin for 24 h and dialyzed samples reacted with streptavidin. (B) ELISA on IgE-coated plates of αD1D2, revealed with anti-SV5 tag mAb or HRP-conjugated streptavidin. (C) Flow cytometry and immunofluorescence microscopy assay with biotinylated (red) or non-biotinylated (violet) αD1D2 on transfected A20 cells expressing human membrane IgE; the green signal corresponds to FITC-conjugated anti-human IgE (ε-chain specific) antibody.

    Techniques Used: Western Blot, Electrophoretic Mobility Shift Assay, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry, Immunofluorescence, Microscopy, Transfection

    Biotinylation dependence on BirA localization . Western blot gel retardation assay on cells co-transfected with plasmid constructs encoding secretory (sec) or cytosolic (cyt) BirA, and scFv 1E10 (A), t-sIgE (B) or NSP5 (C). Where indicated, supernatants (dialyzed) or cell extracts were reacted with streptavidin.
    Figure Legend Snippet: Biotinylation dependence on BirA localization . Western blot gel retardation assay on cells co-transfected with plasmid constructs encoding secretory (sec) or cytosolic (cyt) BirA, and scFv 1E10 (A), t-sIgE (B) or NSP5 (C). Where indicated, supernatants (dialyzed) or cell extracts were reacted with streptavidin.

    Techniques Used: Western Blot, Electrophoretic Mobility Shift Assay, Transfection, Plasmid Preparation, Construct, Size-exclusion Chromatography

    22) Product Images from "Nasal IgE in subjects with allergic and non-allergic rhinitis"

    Article Title: Nasal IgE in subjects with allergic and non-allergic rhinitis

    Journal: The World Allergy Organization Journal

    doi: 10.1016/j.waojou.2020.100129

    Comparison of total IgE and specific IgE to D1 and D2 in nasal secretion between groups. Fig. 3 . Comparison of total IgE (in kilo international units (IU) of IgE per liter (kU/L) and sIgE (in kilo) international units (IU) of allergenspecific antibody per liter (kUA/L) to D1 (Dermatophagoides pteronyssinus) and D2 (Dermatophagoides farinae) in nasal secretion between groups, in log10. Kruskal-Wallis test results regarding the P-values are shown in the graphs
    Figure Legend Snippet: Comparison of total IgE and specific IgE to D1 and D2 in nasal secretion between groups. Fig. 3 . Comparison of total IgE (in kilo international units (IU) of IgE per liter (kU/L) and sIgE (in kilo) international units (IU) of allergenspecific antibody per liter (kUA/L) to D1 (Dermatophagoides pteronyssinus) and D2 (Dermatophagoides farinae) in nasal secretion between groups, in log10. Kruskal-Wallis test results regarding the P-values are shown in the graphs

    Techniques Used:

    23) Product Images from "IgE Levels to Ascaris and House Dust Mite Allergens Are Associated With Increased Histone Acetylation at Key Type-2 Immune Genes"

    Article Title: IgE Levels to Ascaris and House Dust Mite Allergens Are Associated With Increased Histone Acetylation at Key Type-2 Immune Genes

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.00756

    Receiver operating characteristic (ROC) curves and area under the curve (AUC) obtained from logistic regression models to predict the sensitization to HDM allergens. (A) H4 acetylation at the TNFSF13B gene versus IgE sensitization to B. tropicalis . (B) H4 acetylation at the TNFSF13B gene versus IgE sensitization to D. pteronyssinus . (C) H4 acetylation at the TNFSF13B gene versus IgE sensitization to Ascaris spp.
    Figure Legend Snippet: Receiver operating characteristic (ROC) curves and area under the curve (AUC) obtained from logistic regression models to predict the sensitization to HDM allergens. (A) H4 acetylation at the TNFSF13B gene versus IgE sensitization to B. tropicalis . (B) H4 acetylation at the TNFSF13B gene versus IgE sensitization to D. pteronyssinus . (C) H4 acetylation at the TNFSF13B gene versus IgE sensitization to Ascaris spp.

    Techniques Used:

    24) Product Images from "Evaluation of Autologous Serum Skin Test and Skin Prick Test Reactivity to House Dust Mite in Patients with Chronic Spontaneous Urticaria"

    Article Title: Evaluation of Autologous Serum Skin Test and Skin Prick Test Reactivity to House Dust Mite in Patients with Chronic Spontaneous Urticaria

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064142

    Comparison of UAS (A), DLQI (B), total required loratadine every month (C), percentages of associated angioedema (D), duration of disease (F), mean age (F), total IgE (G) and HDM-sIgE (H) among patients with ASST+SPT−, patients with ASST+SPT+, patients with ASST−SPT+ and patients with ASST−SPT−, respectively. * P
    Figure Legend Snippet: Comparison of UAS (A), DLQI (B), total required loratadine every month (C), percentages of associated angioedema (D), duration of disease (F), mean age (F), total IgE (G) and HDM-sIgE (H) among patients with ASST+SPT−, patients with ASST+SPT+, patients with ASST−SPT+ and patients with ASST−SPT−, respectively. * P

    Techniques Used: Single-particle Tracking

    25) Product Images from "Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone, et al. Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone"

    Article Title: Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone, et al. Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone

    Journal: Clinical and Experimental Allergy

    doi: 10.1111/cea.13286

    Receiver operating characteristic ( ROC ) curves for specific antibody levels against peanut extract based test and Ara h 2. A, Predicting the outcome of a positive peanut challenge. B, Predicting outcome of a severe peanut allergy. The P ‐values indicate the difference in performance of IgG, IgG 4 and IgA as compared to IgE
    Figure Legend Snippet: Receiver operating characteristic ( ROC ) curves for specific antibody levels against peanut extract based test and Ara h 2. A, Predicting the outcome of a positive peanut challenge. B, Predicting outcome of a severe peanut allergy. The P ‐values indicate the difference in performance of IgG, IgG 4 and IgA as compared to IgE

    Techniques Used: Acetylene Reduction Assay

    Differences in peanut‐specific IgG/IgE and IgG 4 /IgE ratios. Serum IgG 4 antibody ratios relative to IgE in (A) tolerant vs allergic peanut‐sensitized patients and (B) stratified for the severity of allergic reactions. The symbols and the lines indicate the geometric mean and the 95% confidence interval ( CI ) around that mean
    Figure Legend Snippet: Differences in peanut‐specific IgG/IgE and IgG 4 /IgE ratios. Serum IgG 4 antibody ratios relative to IgE in (A) tolerant vs allergic peanut‐sensitized patients and (B) stratified for the severity of allergic reactions. The symbols and the lines indicate the geometric mean and the 95% confidence interval ( CI ) around that mean

    Techniques Used:

    Variation in IgE and IgG 4 levels to Ara h 2 and in the IgG 4 /IgE ratio. IgE and IgG 4 levels to Ara h 2 are displayed on the left y ‐axis for each patient. All results were converted to ng/mL. On the right y ‐axis, the IgG 4 /IgE ratios are given. Patients were ordered on the x ‐axis from those with low levels to high levels of specific IgE against Ara h 2. The red dots represent allergic subjects and black crosses tolerant. The IgG 4 levels to Ara h 2 for that same patient (same location on the x ‐axis) are indicated as pink dots (allergic) and blue crosses (tolerant). The IgG4/IgE ratios are indicated as green squares (tolerant) and purple diamonds (allergic)
    Figure Legend Snippet: Variation in IgE and IgG 4 levels to Ara h 2 and in the IgG 4 /IgE ratio. IgE and IgG 4 levels to Ara h 2 are displayed on the left y ‐axis for each patient. All results were converted to ng/mL. On the right y ‐axis, the IgG 4 /IgE ratios are given. Patients were ordered on the x ‐axis from those with low levels to high levels of specific IgE against Ara h 2. The red dots represent allergic subjects and black crosses tolerant. The IgG 4 levels to Ara h 2 for that same patient (same location on the x ‐axis) are indicated as pink dots (allergic) and blue crosses (tolerant). The IgG4/IgE ratios are indicated as green squares (tolerant) and purple diamonds (allergic)

    Techniques Used: Acetylene Reduction Assay

    26) Product Images from "Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone, et al. Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone"

    Article Title: Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone, et al. Ratios of specific IgG4 over IgE antibodies do not improve prediction of peanut allergy nor of its severity compared to specific IgE alone

    Journal: Clinical and Experimental Allergy

    doi: 10.1111/cea.13286

    Receiver operating characteristic ( ROC ) curves for specific antibody levels against peanut extract based test and Ara h 2. A, Predicting the outcome of a positive peanut challenge. B, Predicting outcome of a severe peanut allergy. The P ‐values indicate the difference in performance of IgG, IgG 4 and IgA as compared to IgE
    Figure Legend Snippet: Receiver operating characteristic ( ROC ) curves for specific antibody levels against peanut extract based test and Ara h 2. A, Predicting the outcome of a positive peanut challenge. B, Predicting outcome of a severe peanut allergy. The P ‐values indicate the difference in performance of IgG, IgG 4 and IgA as compared to IgE

    Techniques Used: Acetylene Reduction Assay

    27) Product Images from "In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation"

    Article Title: In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI33559

    α-OX40L mAb inhibits OVA-induced inflammation in the lung. ( A ) Experimental design for an acute model of OVA-induced lung inflammation is shown. BALB/c mice (8 per group) were sensitized with OVA/alum or control alum alone on day 0 and challenged with 50 μg OVA administered intranasally daily on days 25–28. Mice were treated with 150 μg control mIgG2a, α-OX40L 4F5 Abs, or dexamethasone (2 mg/kg) intraperitoneally on days 26 and 27. Lungs were analyzed for inflammatory infiltrate ( B ), Th2 cytokines in the BAL ( C ), and serum antigen-specific IgE and IgG1 levels ( D ) on day 29. An increased number of OVA challenges model of lung inflammation is shown in E . BALB/c mice were sensitized with OVA/alum (or control alum alone) on day 0, boosted with OVA on day 14, challenged with OVA on days 49–60, and treated with mIgG2a or α-OX40L 4F5 antibodies intraperitoneally twice a week or 2 mg/kg dexamethasone daily, starting on day 53. Serum antigen-specific IgE and IgG1 levels were measured on day 74 ( F ). Results are mean ± SD. * P
    Figure Legend Snippet: α-OX40L mAb inhibits OVA-induced inflammation in the lung. ( A ) Experimental design for an acute model of OVA-induced lung inflammation is shown. BALB/c mice (8 per group) were sensitized with OVA/alum or control alum alone on day 0 and challenged with 50 μg OVA administered intranasally daily on days 25–28. Mice were treated with 150 μg control mIgG2a, α-OX40L 4F5 Abs, or dexamethasone (2 mg/kg) intraperitoneally on days 26 and 27. Lungs were analyzed for inflammatory infiltrate ( B ), Th2 cytokines in the BAL ( C ), and serum antigen-specific IgE and IgG1 levels ( D ) on day 29. An increased number of OVA challenges model of lung inflammation is shown in E . BALB/c mice were sensitized with OVA/alum (or control alum alone) on day 0, boosted with OVA on day 14, challenged with OVA on days 49–60, and treated with mIgG2a or α-OX40L 4F5 antibodies intraperitoneally twice a week or 2 mg/kg dexamethasone daily, starting on day 53. Serum antigen-specific IgE and IgG1 levels were measured on day 74 ( F ). Results are mean ± SD. * P

    Techniques Used: Mouse Assay

    α-hOX40L mAb inhibits HDM-induced asthma in rhesus monkeys. ( A ) Experimental design for HDM-induced asthma in rhesus monkeys is shown. Young adult monkeys ( n = 6 per group) were sensitized with HDM/alum and boosted periodically with antigen as shown. Monkeys were treated with α-hOX40L mAb LC001 or vehicle control and lung inflammation was analyzed after 22 weeks of treatment. ( B ) TSLP expression in lung biopsies from unsensitized and HDM-sensitized animals are shown. Percentages of lymphocytes and eosinophils ( C ), numbers of effector/memory T cells ( D ), and levels of IL-5 and IL-13 cytokines ( E ) in the BALF are shown. Effector memory T cell numbers (CD4 + CD45RA – CCR7 – ) were assayed by FACS with propidium iodide exclusion for live cells. ( F ) Serum antigen-specific IgG titers (Δ, week 16-baseline pretreatment) are also shown. Results are mean ± SD. * P
    Figure Legend Snippet: α-hOX40L mAb inhibits HDM-induced asthma in rhesus monkeys. ( A ) Experimental design for HDM-induced asthma in rhesus monkeys is shown. Young adult monkeys ( n = 6 per group) were sensitized with HDM/alum and boosted periodically with antigen as shown. Monkeys were treated with α-hOX40L mAb LC001 or vehicle control and lung inflammation was analyzed after 22 weeks of treatment. ( B ) TSLP expression in lung biopsies from unsensitized and HDM-sensitized animals are shown. Percentages of lymphocytes and eosinophils ( C ), numbers of effector/memory T cells ( D ), and levels of IL-5 and IL-13 cytokines ( E ) in the BALF are shown. Effector memory T cell numbers (CD4 + CD45RA – CCR7 – ) were assayed by FACS with propidium iodide exclusion for live cells. ( F ) Serum antigen-specific IgG titers (Δ, week 16-baseline pretreatment) are also shown. Results are mean ± SD. * P

    Techniques Used: Expressing, FACS

    28) Product Images from "Ethyl pyruvate attenuates murine allergic rhinitis partly by decreasing high mobility group box 1 release"

    Article Title: Ethyl pyruvate attenuates murine allergic rhinitis partly by decreasing high mobility group box 1 release

    Journal: Experimental Biology and Medicine

    doi: 10.1177/1535370214566563

    Effect of EP on serum OVA-specific antibodies. The OVA-specific IgE, IgG1, and IgG2a levels were significantly increased in AR and EP treatment groups compared to control group. Treatment with EP at dose of 100 mg/kg significantly reduced the
    Figure Legend Snippet: Effect of EP on serum OVA-specific antibodies. The OVA-specific IgE, IgG1, and IgG2a levels were significantly increased in AR and EP treatment groups compared to control group. Treatment with EP at dose of 100 mg/kg significantly reduced the

    Techniques Used:

    29) Product Images from "IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens"

    Article Title: IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens

    Journal: The Journal of Allergy and Clinical Immunology

    doi: 10.1016/j.jaci.2015.01.012

    IgG 4 /IgE ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test.
    Figure Legend Snippet: IgG 4 /IgE ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test.

    Techniques Used: MANN-WHITNEY

    30) Product Images from "Biosignature for airway inflammation in a house dust mite-challenged murine model of allergic asthma"

    Article Title: Biosignature for airway inflammation in a house dust mite-challenged murine model of allergic asthma

    Journal: Biology Open

    doi: 10.1242/bio.014464

    HDM challenge increases immunoglobulin levels. 8-10-week-old female Balb/c mice were challenged by intranasal administration of 35 μl of whole HDM extract (0.7 mg/ml) in saline, for 2 weeks. Immunoglobulin levels, (A) total IgE, (B) total IgG, (C) HDM-specific IgE and (D) HDM-specific IgG1, were monitored in serum from naïve ( n =8) and HDM-challenged ( n =8) mice, 24 h after the last HDM challenge, by ELISA. Results are shown as box plots with median line, and statistical analyses was performed using the Mann-Whitney U-test (** P ≤0.005, *** P ≤0.0005).
    Figure Legend Snippet: HDM challenge increases immunoglobulin levels. 8-10-week-old female Balb/c mice were challenged by intranasal administration of 35 μl of whole HDM extract (0.7 mg/ml) in saline, for 2 weeks. Immunoglobulin levels, (A) total IgE, (B) total IgG, (C) HDM-specific IgE and (D) HDM-specific IgG1, were monitored in serum from naïve ( n =8) and HDM-challenged ( n =8) mice, 24 h after the last HDM challenge, by ELISA. Results are shown as box plots with median line, and statistical analyses was performed using the Mann-Whitney U-test (** P ≤0.005, *** P ≤0.0005).

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    31) Product Images from "Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects"

    Article Title: Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects

    Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    doi: 10.1111/pai.12310

    Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p
    Figure Legend Snippet: Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Techniques Used: Microarray

    32) Product Images from "Systemically derived large intestinal CD4+ Th2 cells play a central role in STAT6-mediated allergic diarrhea"

    Article Title: Systemically derived large intestinal CD4+ Th2 cells play a central role in STAT6-mediated allergic diarrhea

    Journal: Journal of Clinical Investigation

    doi:

    Selective induction of brisk B-cell responses in the large intestine of mice with diarrhea. ( a ) Total and OVA-specific IgE Ab’s in serum and total IgE AFCs in the small and large intestines. For evaluation of the numbers of IgE positive–staining cells in the large intestine, ten randomly selected fields were counted under ×200. ( b ) Represents OVA-specific IgG and IgA AFCs in the spleen and in the small and large intestines. The inset shows the frequency of OVA-specific IgG subclass AFCs in the large intestines of mice with diarrhea. Mononuclear cells were isolated from spleen and small and large intestines of control (open bars) and diarrhea-induced mice (filled bars). These data are representative of three independent experiments containing five to seven mice in each group. A P
    Figure Legend Snippet: Selective induction of brisk B-cell responses in the large intestine of mice with diarrhea. ( a ) Total and OVA-specific IgE Ab’s in serum and total IgE AFCs in the small and large intestines. For evaluation of the numbers of IgE positive–staining cells in the large intestine, ten randomly selected fields were counted under ×200. ( b ) Represents OVA-specific IgG and IgA AFCs in the spleen and in the small and large intestines. The inset shows the frequency of OVA-specific IgG subclass AFCs in the large intestines of mice with diarrhea. Mononuclear cells were isolated from spleen and small and large intestines of control (open bars) and diarrhea-induced mice (filled bars). These data are representative of three independent experiments containing five to seven mice in each group. A P

    Techniques Used: Mouse Assay, Staining, Isolation

    33) Product Images from "The IgE response to Ascaris molecular components is associated with clinical indicators of asthma severity"

    Article Title: The IgE response to Ascaris molecular components is associated with clinical indicators of asthma severity

    Journal: The World Allergy Organization Journal

    doi: 10.1186/s40413-015-0058-z

    IgE responses to HDM-specific allergens according to Ascaris sensitization. IgE response to Der p 2, a specific marker for Dermatophagoides spp. and Blo t 5, a specific marker for Blomia tropicalis among Ascaris sensitized and non sensitized (Ascaris neg.) subjects. * p
    Figure Legend Snippet: IgE responses to HDM-specific allergens according to Ascaris sensitization. IgE response to Der p 2, a specific marker for Dermatophagoides spp. and Blo t 5, a specific marker for Blomia tropicalis among Ascaris sensitized and non sensitized (Ascaris neg.) subjects. * p

    Techniques Used: Marker

    Correlation among Ascaris , B. tropicalis and D. pteronyssinus - specific IgE values. Log transformed values were represented in this superimposed dispersion plot; linear regression slopes for B. tropicalis – Ascaris (black) and D. pteronyssinus – Ascaris (grey) [X-Y] pairs are also shown.
    Figure Legend Snippet: Correlation among Ascaris , B. tropicalis and D. pteronyssinus - specific IgE values. Log transformed values were represented in this superimposed dispersion plot; linear regression slopes for B. tropicalis – Ascaris (black) and D. pteronyssinus – Ascaris (grey) [X-Y] pairs are also shown.

    Techniques Used: Transformation Assay

    34) Product Images from "IgG1 memory B cells keep the memory of IgE responses"

    Article Title: IgG1 memory B cells keep the memory of IgE responses

    Journal: Nature Communications

    doi: 10.1038/s41467-017-00723-0

    Early and late generation of IgE PC from different IgG1 MBC subsets. The phenotype of IgG1 and IgE cells generated from IgG1 MBC was analysed. IgG1 MBC subsets isolated from OVA-PEP1 immunised TBmc mice, were transferred together with CD4 memory T cells into Rag1 KO recipients. The recipient mice were immunised with OVA-PEP1. One and 6 weeks later spleen cells were collected, stained with antibodies to B220, CD138, GL7, PDL2, IgE, IgG1 and the proliferation antigen Ki67, and analysed by flow cytometry. a , b Left : frequency of IgE + B220 + and IgG1 + B220 + cells among total B220 + CD138 − cells; middle : frequency of GL7 + GC cells and PDL2 + memory B cells among B220 + CD138 − IgG1 + cells; right : frequency of IgE + PC and IgG1 + PC among B220 − CD138 + PC. c Percentage of PC, GC cells and memory B cells among total IgE + cells ( left ) and total IgG1 + cells ( right ) at 1 week after transfer/immunisation. d Proliferation analysis of splenic IgE + and IgG1 + cells of recipient mice at weeks 1 and 6 after transfer/immunisation. e Number of IgE + PC, IgG1 + PC, IgG1 + GC and IgG1 + MBC per spleen in recipient mice at weeks 1 and 6 after transfer/immunisation. Each dot represents one recipient mouse. Average of 4 (DP + T, week 1), 5 (DP + T, week 6), 3 (DN + T, week 1) and 3 (DN + T, week 6) mice per group were shown. Statistical analysis was performed using Mann–Whitney–Wilcoxon U -test. * P
    Figure Legend Snippet: Early and late generation of IgE PC from different IgG1 MBC subsets. The phenotype of IgG1 and IgE cells generated from IgG1 MBC was analysed. IgG1 MBC subsets isolated from OVA-PEP1 immunised TBmc mice, were transferred together with CD4 memory T cells into Rag1 KO recipients. The recipient mice were immunised with OVA-PEP1. One and 6 weeks later spleen cells were collected, stained with antibodies to B220, CD138, GL7, PDL2, IgE, IgG1 and the proliferation antigen Ki67, and analysed by flow cytometry. a , b Left : frequency of IgE + B220 + and IgG1 + B220 + cells among total B220 + CD138 − cells; middle : frequency of GL7 + GC cells and PDL2 + memory B cells among B220 + CD138 − IgG1 + cells; right : frequency of IgE + PC and IgG1 + PC among B220 − CD138 + PC. c Percentage of PC, GC cells and memory B cells among total IgE + cells ( left ) and total IgG1 + cells ( right ) at 1 week after transfer/immunisation. d Proliferation analysis of splenic IgE + and IgG1 + cells of recipient mice at weeks 1 and 6 after transfer/immunisation. e Number of IgE + PC, IgG1 + PC, IgG1 + GC and IgG1 + MBC per spleen in recipient mice at weeks 1 and 6 after transfer/immunisation. Each dot represents one recipient mouse. Average of 4 (DP + T, week 1), 5 (DP + T, week 6), 3 (DN + T, week 1) and 3 (DN + T, week 6) mice per group were shown. Statistical analysis was performed using Mann–Whitney–Wilcoxon U -test. * P

    Techniques Used: Generated, Isolation, Mouse Assay, Staining, Flow Cytometry, Cytometry, MANN-WHITNEY

    IgE derived from DP MBC mediates anaphylaxis. The pathogenic capacity of IgE derived from DP and DN IgG1 MBC was evaluated in a passive cutaneous anaphylaxis (PCA) assay. IgG-depleted serum was obtained from Rag1 KO recipient mice that had been transfused with DP or DN IgG1 + MBC and CD4 memory T cells from primary OVA-PEP1 immunised TBmc mice as described in Fig. 2a, c, d . The recipient mice were immunised with OVA-PEP1, and serum was obtained at 1 and 6 weeks after transfer/immunisation. Serum from each group was pooled and depleted of IgG antibodies. For the PCA assay, 20 μl of serum was injected intradermally into the ears of naive BALB/c mice. Twenty-four hours later, 50 µg OVA-PEP1 and 1% Evans Blue in PBS were injected intravenously. Thirty minutes later, the anaphylactic reaction was evaluated visually. a , b Sera from recipients of DP IgG1 MBC (DP-serum), but not from recipients of DN IgG1 MBC (DN-serum), mediated anaphylaxis. a One-week sera. b Six-week sera. c To determine if the DN-serum could inhibit anaphylaxis, 6-week DP-serum was diluted with 6-week DN-serum, or with serum from untreated Rag1 KO mice (RKO serum) at the indicated DP:DN and DP:RKO ratios (1:125; 1:250; 1:500). The mixed serum anaphylactic activity was measured in a PCA assay. The data are representative of two experiments
    Figure Legend Snippet: IgE derived from DP MBC mediates anaphylaxis. The pathogenic capacity of IgE derived from DP and DN IgG1 MBC was evaluated in a passive cutaneous anaphylaxis (PCA) assay. IgG-depleted serum was obtained from Rag1 KO recipient mice that had been transfused with DP or DN IgG1 + MBC and CD4 memory T cells from primary OVA-PEP1 immunised TBmc mice as described in Fig. 2a, c, d . The recipient mice were immunised with OVA-PEP1, and serum was obtained at 1 and 6 weeks after transfer/immunisation. Serum from each group was pooled and depleted of IgG antibodies. For the PCA assay, 20 μl of serum was injected intradermally into the ears of naive BALB/c mice. Twenty-four hours later, 50 µg OVA-PEP1 and 1% Evans Blue in PBS were injected intravenously. Thirty minutes later, the anaphylactic reaction was evaluated visually. a , b Sera from recipients of DP IgG1 MBC (DP-serum), but not from recipients of DN IgG1 MBC (DN-serum), mediated anaphylaxis. a One-week sera. b Six-week sera. c To determine if the DN-serum could inhibit anaphylaxis, 6-week DP-serum was diluted with 6-week DN-serum, or with serum from untreated Rag1 KO mice (RKO serum) at the indicated DP:DN and DP:RKO ratios (1:125; 1:250; 1:500). The mixed serum anaphylactic activity was measured in a PCA assay. The data are representative of two experiments

    Techniques Used: Derivative Assay, Mouse Assay, Injection, Activity Assay

    CD73 + CD80 + IgG1 MBC give rise to high-affinity IgE. a Diagram of adoptive transfer experiments to evaluate IgE production from IgG1 MBC subsets (i.v.: intravenously). b Donor-derived IgE a and IgG1 a production in CB17 IgH b recipient mice transferred with IgG1 + MBC (DP, SP and DN) and CD4 memory T cells (T) from N.brasiliensis (N.b.) infected mice, and subsequently infected with N.b . IgE a and IgG1 a serum levels were determined at 2 weeks after transfer and infection. The data shows average of n = 8 for DP + T, n = 3 for SP + T, n = 3 for DN + T, or n = 7 for T alone, n = 7 for N.b . and n = 7 for untreated (UT) groups. The samples were pooled from three independent experiments. Statistical analysis was performed using Mann–Whitney–Wilcoxon U -test. * P
    Figure Legend Snippet: CD73 + CD80 + IgG1 MBC give rise to high-affinity IgE. a Diagram of adoptive transfer experiments to evaluate IgE production from IgG1 MBC subsets (i.v.: intravenously). b Donor-derived IgE a and IgG1 a production in CB17 IgH b recipient mice transferred with IgG1 + MBC (DP, SP and DN) and CD4 memory T cells (T) from N.brasiliensis (N.b.) infected mice, and subsequently infected with N.b . IgE a and IgG1 a serum levels were determined at 2 weeks after transfer and infection. The data shows average of n = 8 for DP + T, n = 3 for SP + T, n = 3 for DN + T, or n = 7 for T alone, n = 7 for N.b . and n = 7 for untreated (UT) groups. The samples were pooled from three independent experiments. Statistical analysis was performed using Mann–Whitney–Wilcoxon U -test. * P

    Techniques Used: Adoptive Transfer Assay, Derivative Assay, Mouse Assay, Infection, MANN-WHITNEY

    High affinity PC are selected from pre-existent memory clones. The repertoires of VDJ sequences from donor IgG1 MBC subsets (from 10 weeks OVA-PEP1 immunised TBmc mice) and their IgG1 and IgE progenies in spleen and BM of recipient mice (2 weeks after transfer/immunisation), were used to investigate the precursor-progeny relationship. a In silico derived sublibrary of the top 20 (frequency descending) VDJ CDR3 aa sequences containing high affinity mutations (in red ). b Radar charts show the presence and frequency of each of the top 20 high affinity CDR3 sequences in donor DP IgG1 MBC and in the progeny IgE ( left ) and IgG1 ( right ) libraries. a , b The data are from one representative mouse of three recipient mice. c Percentage of the progeny IgE and IgG1 nucleotide sequences (nt seq) encoding high affinity CDR3 that were found in the donor DP IgG1 MBC. M1– M3 indicate individual recipient mice
    Figure Legend Snippet: High affinity PC are selected from pre-existent memory clones. The repertoires of VDJ sequences from donor IgG1 MBC subsets (from 10 weeks OVA-PEP1 immunised TBmc mice) and their IgG1 and IgE progenies in spleen and BM of recipient mice (2 weeks after transfer/immunisation), were used to investigate the precursor-progeny relationship. a In silico derived sublibrary of the top 20 (frequency descending) VDJ CDR3 aa sequences containing high affinity mutations (in red ). b Radar charts show the presence and frequency of each of the top 20 high affinity CDR3 sequences in donor DP IgG1 MBC and in the progeny IgE ( left ) and IgG1 ( right ) libraries. a , b The data are from one representative mouse of three recipient mice. c Percentage of the progeny IgE and IgG1 nucleotide sequences (nt seq) encoding high affinity CDR3 that were found in the donor DP IgG1 MBC. M1– M3 indicate individual recipient mice

    Techniques Used: Clone Assay, Mouse Assay, In Silico, Derivative Assay

    PC derived from DP IgG1 MBC are enriched in high affinity mutations. VDJ nucleotide (nt) and amino acid (aa) sequences were compared between IgG1 MBC subsets and their IgE and IgG1 progeny cells. IgG1 MBC subsets were purified from 10 weeks OVA-PEP1 immunised TBmc mice, and transferred together with CD4 memory T cells into Rag1 KO recipient mice. The recipient mice were immunised with OVA-PEP1. Spleen and bone marrow (BM) of the recipient mice were collected 2 weeks later. The VDJ repertoires of parental IgG1 MBC subsets and their IgG1 and IgE progeny were analysed using next generation sequencing. a , b Average number of nt a and aa b mutations per sequence. c Percentage of sequences containing R97T, N100aS/T or A101T high affinity CDR3 mutations. a – c Data are average ± SEM of three mice per group. d Enrichment in the percentage of CDR3 sequences containing 2 or 3 high affinity aa per sequence (hi aff aa/seq) in the IgE and IgG1 progeny of DP IgG1 MBC (M1, M2 and M3 indicate three recipient mice). Data are representative of two independent experiments
    Figure Legend Snippet: PC derived from DP IgG1 MBC are enriched in high affinity mutations. VDJ nucleotide (nt) and amino acid (aa) sequences were compared between IgG1 MBC subsets and their IgE and IgG1 progeny cells. IgG1 MBC subsets were purified from 10 weeks OVA-PEP1 immunised TBmc mice, and transferred together with CD4 memory T cells into Rag1 KO recipient mice. The recipient mice were immunised with OVA-PEP1. Spleen and bone marrow (BM) of the recipient mice were collected 2 weeks later. The VDJ repertoires of parental IgG1 MBC subsets and their IgG1 and IgE progeny were analysed using next generation sequencing. a , b Average number of nt a and aa b mutations per sequence. c Percentage of sequences containing R97T, N100aS/T or A101T high affinity CDR3 mutations. a – c Data are average ± SEM of three mice per group. d Enrichment in the percentage of CDR3 sequences containing 2 or 3 high affinity aa per sequence (hi aff aa/seq) in the IgE and IgG1 progeny of DP IgG1 MBC (M1, M2 and M3 indicate three recipient mice). Data are representative of two independent experiments

    Techniques Used: Derivative Assay, Purification, Mouse Assay, Next-Generation Sequencing, Sequencing

    35) Product Images from "Adam8 Limits the Development of Allergic Airway Inflammation in Mice"

    Article Title: Adam8 Limits the Development of Allergic Airway Inflammation in Mice

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1202329

    OVA-treated Adam8 −/− mice have higher lung levels of T H 2 cytokines, Rantes, Ip-10, and Tnf-α compared with OVA-treated WT mice
    Figure Legend Snippet: OVA-treated Adam8 −/− mice have higher lung levels of T H 2 cytokines, Rantes, Ip-10, and Tnf-α compared with OVA-treated WT mice

    Techniques Used: Mouse Assay

    36) Product Images from "Inhibitory Effect of Loranthus parasiticus on IgE-Mediated Allergic Responses in RBL-2H3 Cells"

    Article Title: Inhibitory Effect of Loranthus parasiticus on IgE-Mediated Allergic Responses in RBL-2H3 Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2016/8742562

    Inhibitory effect of LPE on proinflammatory cytokines. IgE-sensitized RBL-2H3 cells were preincubated with LPE for 1 h prior to antigen challenge. TNF- α and IL-4 levels were determined as described in Section 2 . Data are mean ± SD values of triple determinations. ∗∗ P
    Figure Legend Snippet: Inhibitory effect of LPE on proinflammatory cytokines. IgE-sensitized RBL-2H3 cells were preincubated with LPE for 1 h prior to antigen challenge. TNF- α and IL-4 levels were determined as described in Section 2 . Data are mean ± SD values of triple determinations. ∗∗ P

    Techniques Used:

    37) Product Images from "Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay"

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0035697

    ABA versus ISAC correlation results on serum samples selected on the basis of the allergen specificities reported in each panel and listed in Table S1 . Letter flags, namely B, C, D, E, F, G, in figure 6 indicate them as parts of the results shown also in figures 5 , 7 , and 8 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero value for ABA was set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC value on the Y axis. The Spearman r correlation coefficient was calculated and the Fisher's exact test was used for statistical purposes. Statistical results are reported below each graph.
    Figure Legend Snippet: ABA versus ISAC correlation results on serum samples selected on the basis of the allergen specificities reported in each panel and listed in Table S1 . Letter flags, namely B, C, D, E, F, G, in figure 6 indicate them as parts of the results shown also in figures 5 , 7 , and 8 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero value for ABA was set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC value on the Y axis. The Spearman r correlation coefficient was calculated and the Fisher's exact test was used for statistical purposes. Statistical results are reported below each graph.

    Techniques Used: Recombinant

    ABA versus ISAC correlation results on serum samples selected on the basis of the allergen specificities reported in each panel and listed in Table S1 . Letter flags, namely N, O, P, Q, in figure 8 indicate them as parts of the results shown also in figures 5 , 6 , and 7 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero value for ABA was set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC value on the Y axis. The Spearman r correlation coefficient was calculated and the Fisher's exact test was used for statistical purposes. Statistical results are reported below each graph.
    Figure Legend Snippet: ABA versus ISAC correlation results on serum samples selected on the basis of the allergen specificities reported in each panel and listed in Table S1 . Letter flags, namely N, O, P, Q, in figure 8 indicate them as parts of the results shown also in figures 5 , 6 , and 7 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero value for ABA was set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC value on the Y axis. The Spearman r correlation coefficient was calculated and the Fisher's exact test was used for statistical purposes. Statistical results are reported below each graph.

    Techniques Used: Recombinant

    Selected examples of Allergen micro-Beads Array (ABA) IgE testing report. Eleven different micro-beads were tested in the same tube in each run for each sample. Examples were selected on purpose among the samples with high total IgE. All IgE positive and negative ABA results matched the ISAC results. Numbers in the upper left corner indicate the Table S1 row numbers and patients' ID. In each panel: the upper left graph shows clustered micro-beads by their dimension; the upper right: scatter plots of each fluorescent bead; the lower left: fluorescence intensity and event counts; the lower right: summary table with median fluorescence values. Samples reported in each of the six panels had the following total IgE values: Panel A = 9,730 IU/l; Panel B = 1,351 IU/l; Panel C = 1,220 IU/l; Panel D = 1,931 IU/l; Panel E = 20,900 IU/l; Panel F = 23,540 IU/l.
    Figure Legend Snippet: Selected examples of Allergen micro-Beads Array (ABA) IgE testing report. Eleven different micro-beads were tested in the same tube in each run for each sample. Examples were selected on purpose among the samples with high total IgE. All IgE positive and negative ABA results matched the ISAC results. Numbers in the upper left corner indicate the Table S1 row numbers and patients' ID. In each panel: the upper left graph shows clustered micro-beads by their dimension; the upper right: scatter plots of each fluorescent bead; the lower left: fluorescence intensity and event counts; the lower right: summary table with median fluorescence values. Samples reported in each of the six panels had the following total IgE values: Panel A = 9,730 IU/l; Panel B = 1,351 IU/l; Panel C = 1,220 IU/l; Panel D = 1,931 IU/l; Panel E = 20,900 IU/l; Panel F = 23,540 IU/l.

    Techniques Used: Fluorescence

    ABA versus ISAC IgE correlation results on 1,519 serum samples selected on the basis of any of the allergen specificities reported in Table S1 , using the two micro systems. Letter flag (A) in figure 5 indicates it as part of the results shown also in figures 6 , 7 , and 8 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero values for ABA were set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC values on the Y axis. The Spearman r correlation coefficient was calculated and the χ 2 test was used for statistical purposes. Statistical results are reported below the graph.
    Figure Legend Snippet: ABA versus ISAC IgE correlation results on 1,519 serum samples selected on the basis of any of the allergen specificities reported in Table S1 , using the two micro systems. Letter flag (A) in figure 5 indicates it as part of the results shown also in figures 6 , 7 , and 8 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero values for ABA were set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC values on the Y axis. The Spearman r correlation coefficient was calculated and the χ 2 test was used for statistical purposes. Statistical results are reported below the graph.

    Techniques Used: Recombinant

    ABA versus ISAC correlation results on serum samples selected on the basis of the allergen specificities reported in each panel and listed in Table S1 . Letter flags, namely H, I, J, K, L, M, in figure 7 indicate them as parts of the results shown also in figures 5 , 6 , and 8 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero value for ABA was set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC value on the Y axis. The Spearman r correlation coefficient was calculated and the Fisher's exact test was used for statistical purposes. Statistical results are reported below each graph.
    Figure Legend Snippet: ABA versus ISAC correlation results on serum samples selected on the basis of the allergen specificities reported in each panel and listed in Table S1 . Letter flags, namely H, I, J, K, L, M, in figure 7 indicate them as parts of the results shown also in figures 5 , 6 , and 8 . Consecutive letters are used on purpose to recall result type continuity across the four figures. Allergen nature, being either natural or recombinant, matched for both tests. Vertical dashed lines represent the arbitrary ABA negative cut off value. Horizontal dashed lines mark the value range where ISAC IgE determinations are not always reproducible (unpublished data). For graphical visualization needs on log scales, zero value for ABA was set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC value on the Y axis. The Spearman r correlation coefficient was calculated and the Fisher's exact test was used for statistical purposes. Statistical results are reported below each graph.

    Techniques Used: Recombinant

    ABA versus ISAC correlation results on 137 serum samples selected on the basis of nDer s 1, nPen m 1, and nPru p 3 mutually exclusive IgE positivity are reported. Panel A: All 411 IgE values, obtained by testing the three allergens; Panel B: 137 IgE results obtained on nDer s 1 allergen; Panel C: 137 IgE results obtained on nPen m 1 allergen; Panel D: 137 IgE results obtained on nPru p 3 allergen. For graphical visualization needs on log scales, zero values for ABA were set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC values on the Y axis. The Spearman r correlation coefficient, the χ 2 and the Fisher's exact tests were used where applicable.
    Figure Legend Snippet: ABA versus ISAC correlation results on 137 serum samples selected on the basis of nDer s 1, nPen m 1, and nPru p 3 mutually exclusive IgE positivity are reported. Panel A: All 411 IgE values, obtained by testing the three allergens; Panel B: 137 IgE results obtained on nDer s 1 allergen; Panel C: 137 IgE results obtained on nPen m 1 allergen; Panel D: 137 IgE results obtained on nPru p 3 allergen. For graphical visualization needs on log scales, zero values for ABA were set at 10 MFI on the X axis, and at 0.01 kU/l for ISAC values on the Y axis. The Spearman r correlation coefficient, the χ 2 and the Fisher's exact tests were used where applicable.

    Techniques Used:

    38) Product Images from "Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets"

    Article Title: Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2019.02705

    Representation of 3 color fluorescence immunohistology of CD4 (red), CD25 (blue), Foxp3 (green), and CD4CD25 (magenta) positive staining in the distal jejunum lamina propria of a 28 day old female and male control (no dietary supplementation) piglets (A) . Bar = 10 μm. Quantitative analysis (log transformed) of CD4 + CD25 + Foxp3 + cells/mm 2 of distal jejunum lamina propria in 28 day old piglets by treatment (unsupplemented control, inulin or starch supplemented) and by sex (females = black bars, males = white bars) (B) . Ten individual images were analyzed for each sample. * p
    Figure Legend Snippet: Representation of 3 color fluorescence immunohistology of CD4 (red), CD25 (blue), Foxp3 (green), and CD4CD25 (magenta) positive staining in the distal jejunum lamina propria of a 28 day old female and male control (no dietary supplementation) piglets (A) . Bar = 10 μm. Quantitative analysis (log transformed) of CD4 + CD25 + Foxp3 + cells/mm 2 of distal jejunum lamina propria in 28 day old piglets by treatment (unsupplemented control, inulin or starch supplemented) and by sex (females = black bars, males = white bars) (B) . Ten individual images were analyzed for each sample. * p

    Techniques Used: Fluorescence, Staining, Transformation Assay

    39) Product Images from "Specific immunotherapy ameliorates ulcerative colitis"

    Article Title: Specific immunotherapy ameliorates ulcerative colitis

    Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

    doi: 10.1186/s13223-016-0142-0

    Effect of the therapy on serum IgE, IgG4 and CD4 + T cell cytokines. Peripheral blood samples were collected from each patient before and after the therapy. The sera were analyzed by ELISA. The bars indicate the serum levels of SIgE ( a ), SIgG4 ( b ), IL-4 ( c ), IFN-g ( d ), IL-17 ( e ) and TNF-alpha ( f ). The data are presented as mean ± SD. *p
    Figure Legend Snippet: Effect of the therapy on serum IgE, IgG4 and CD4 + T cell cytokines. Peripheral blood samples were collected from each patient before and after the therapy. The sera were analyzed by ELISA. The bars indicate the serum levels of SIgE ( a ), SIgG4 ( b ), IL-4 ( c ), IFN-g ( d ), IL-17 ( e ) and TNF-alpha ( f ). The data are presented as mean ± SD. *p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    40) Product Images from "Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study"

    Article Title: Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2015.09.052

    A and B , Likelihood ( y-axis , percentage) of reporting symptoms to cat at 16 years of age depending of the number of IgE-reactive cat allergens (Fel d 1, Fel d 2, and Fel d 4) and ImmunoCAP cat extract sensitization (x-axes) at 4 (Fig 5, A ) and 8 (Fig 5, B ) years of age. C and D , Likelihood ( y-axis , percentage) of reporting symptoms to dog at 16 years of age depending of the number of IgE-reactive dog allergens (Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6) and ImmunoCAP dog extract sensitization (x-axes) at 4 (Fig 5, C ) and 8 (Fig 5, D ) years of age. Numbers, ORs, and 95% CIs are shown (n = 779).
    Figure Legend Snippet: A and B , Likelihood ( y-axis , percentage) of reporting symptoms to cat at 16 years of age depending of the number of IgE-reactive cat allergens (Fel d 1, Fel d 2, and Fel d 4) and ImmunoCAP cat extract sensitization (x-axes) at 4 (Fig 5, A ) and 8 (Fig 5, B ) years of age. C and D , Likelihood ( y-axis , percentage) of reporting symptoms to dog at 16 years of age depending of the number of IgE-reactive dog allergens (Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6) and ImmunoCAP dog extract sensitization (x-axes) at 4 (Fig 5, C ) and 8 (Fig 5, D ) years of age. Numbers, ORs, and 95% CIs are shown (n = 779).

    Techniques Used:

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    Purification:

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    Incubation:

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    Protein Concentration:

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    Thermo Fisher serum specific ige
    IgG 4 <t>/IgE</t> ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test.
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    IgG 4 /IgE ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test.

    Journal: The Journal of Allergy and Clinical Immunology

    Article Title: IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens

    doi: 10.1016/j.jaci.2015.01.012

    Figure Lengend Snippet: IgG 4 /IgE ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test.

    Article Snippet: Serum specific IgE and IgG4 levels to peanut and peanut components Serum specific IgE and IgG4 to peanut extract and to the recombinant peanut allergens rAra h 1, rAra h 2, rAra h 3, rAra h 8, and rAra h 9 were measured with an immunoenzymatic assay (ImmunoCAP; Thermo Fisher, Waltham, Mass).

    Techniques: MANN-WHITNEY

    Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    Article Title: Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects

    doi: 10.1111/pai.12310

    Figure Lengend Snippet: Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Article Snippet: Serum-specific IgE and IgG antibody levels were measured at the same time points with the use of the Immuno-CAP® (Thermo Fisher Scientific-Phadia AB and chip-based microarray [Thermo Fisher Scientific-Phadia AB ( )].

    Techniques: Microarray