Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Hepatocyte Smoothened Activity Controls Susceptibility to Insulin Resistance and Nonalcoholic Fatty Liver Disease

doi: 10.1016/j.jcmgh.2022.12.008

Figure Lengend Snippet: List of Antibodies for Western Blotting and IHC

Article Snippet: pAkt Ser 473 , Cell Signaling , Cat. #4060 , 1:1000.

Techniques: Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Inhibition of Xanthine Oxidase Protects against Diabetic Kidney Disease through the Amelioration of Oxidative Stress via VEGF/VEGFR Axis and NOX-FoxO3a-eNOS Signaling Pathway

doi: 10.3390/ijms24043807

Figure Lengend Snippet: Representative images and quantitative analysis for Akt, FoxOs and eNOS expressions in STZ-induced diabetic mice with or without febuxostat treatment. ( a ) Representative immunoblot showing Akt, FoxOs and eNOS expression levels in mouse kidneys. ( b ) Quantitative analyses for phosphor-Ser 473 Akt/total Akt, ( c ) Quantitative analyses for phospho-Ser 256 FoxO1/total-FoxO1. ( d ) Quantitative analyses for phospho-Ser 253 FoxO3a/total-FoxO3a. ( e ) Quantitative analyses for phospho-Ser 1173 eNOS/total eNOS, * p < 0.05 and *** p < 0.001 vs. Cont, †† p < 0.01 and ††† p < 0.001 vs. Feb and ‡ p < 0.05, ‡‡ p < 0.01, and ‡‡‡ p < 0.001 vs. STZ group.

Article Snippet: The membrane was incubated with primary antibodies against the following target proteins: VEGFR1 (Boster, Pleasanton, CA, USA), VEGFR2 (St John’s Laboratory, London, UK), VEGFR3 (NSJ Bioreagent Logo, San Diego, CA, USA), NOX1 and NOX4 (Lifespan Biosciences, Seattle, WA, USA), NOX2 (BD Biosciences, San Jose, CA, USA), total Akt, phospho-Ser 473 Akt, SOD1 and SOD2 (Cell Signaling Technology, Danvers, MA, USA), total FoxO1, phospho-Ser 256 FoxO1, total FoxO3a, and phospho-Ser 253 FoxO3a (Novus Biologicals, Centennial, CO, USA), total endothelial nitric oxide synthase (eNOS, EMD Millipore, Middlesex County, MA, USA), and phospho-Ser 1173 eNOS (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot, Expressing