epityper of the sequenom massarray platform (Sequenom)
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Epityper Of The Sequenom Massarray Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Methylation Sequencing:Article Title: Stat3 is a candidate epigenetic biomarker of perinatal Bisphenol A exposure associated with murine hepatic tumors with implications for human health. Article Snippet: Increased production of the cytokine IL-6 initiates downstream STAT3 signaling.18,22 STAT3 signaling is a key event in inflammation-driven liver carcinogenesis.18 In fact, the classical sexual dimorphism in HCC incidence is thought to be due to the anti-inflammatory influence of endogenous estrogen via suppression of IL-6 production in an ERα-dependent manner.23 BPA has been shown to increase expression of both IL-6 and STAT3 via both ERα and ERβ in human hepatoma D ow nl oa de d by [ U ni ve rs ity o f N eb ra sk a, L in co ln ] at 1 4: 53 0 9 D ec em be r 20 15 Hep3b cells.23 In order to identify short, defined regions for quantitative DNA methylation analysis within these candidate genes, we mined existing whole genome next generation sequencing DNA methylation datasets for windows that exhibited lability in DNA methylation profiles following perinatal BPA exposure in both mice24 and humans (unpublished data, in review) (MethylPlex technology, Rubicon Genomics, Ann Arbor, MI). .. This approach allowed us to combine a traditional candidate gene approach, suitable for highly quantitative bisulfite sequencing on the Methylation:Article Title: Stat3 is a candidate epigenetic biomarker of perinatal Bisphenol A exposure associated with murine hepatic tumors with implications for human health. Article Snippet: Increased production of the cytokine IL-6 initiates downstream STAT3 signaling.18,22 STAT3 signaling is a key event in inflammation-driven liver carcinogenesis.18 In fact, the classical sexual dimorphism in HCC incidence is thought to be due to the anti-inflammatory influence of endogenous estrogen via suppression of IL-6 production in an ERα-dependent manner.23 BPA has been shown to increase expression of both IL-6 and STAT3 via both ERα and ERβ in human hepatoma D ow nl oa de d by [ U ni ve rs ity o f N eb ra sk a, L in co ln ] at 1 4: 53 0 9 D ec em be r 20 15 Hep3b cells.23 In order to identify short, defined regions for quantitative DNA methylation analysis within these candidate genes, we mined existing whole genome next generation sequencing DNA methylation datasets for windows that exhibited lability in DNA methylation profiles following perinatal BPA exposure in both mice24 and humans (unpublished data, in review) (MethylPlex technology, Rubicon Genomics, Ann Arbor, MI). .. This approach allowed us to combine a traditional candidate gene approach, suitable for highly quantitative bisulfite sequencing on the |

