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Illumina Inc sequencing oligos
Sequencing Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequencing oligos/product/Illumina Inc
Average 94 stars, based on 5 article reviews
Price from $9.99 to $1999.99
sequencing oligos - by Bioz Stars, 2020-04
94/100 stars

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hiseq 2500 platform
srnas
truseq small rna sample kit

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Related Articles

Multiplex Assay:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: Diluted (1:20) amplified DNA was used in single or multiplex PCR using primers relevant to the sample and high Fidelity Phusion Taq polymerase (NEB, UK) or KAPA2G Multiplex DNA Polymerase (KAPA Biosystems, UK). .. Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK).

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: Targeted PCR was performed using high Fidelity Phusion Taq polymerase (NEB, UK) or KAPA2G Multiplex DNA Polymerase (KAPA Biosystems, UK) with 10ng of gDNA. .. A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK).

Amplification:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: Diluted (1:20) amplified DNA was used in single or multiplex PCR using primers relevant to the sample and high Fidelity Phusion Taq polymerase (NEB, UK) or KAPA2G Multiplex DNA Polymerase (KAPA Biosystems, UK). .. Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK).

RNA Sequencing Assay:

Article Title: Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population
Article Snippet: Paragraph title: PLANT MATERIALS, VIRUS, RNA EXTRACTION, AND SMALL RNA SEQUENCING ... Subsequently, libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform.

Sequencing:

Article Title: A Short Indel-Lacking-Resistance Gene Triggers Silencing of the Photosynthetic Machinery Components Through TYLCSV-Associated Endogenous siRNAs in Tomato
Article Snippet: .. Libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Differential expression of selected transcripts was confirmed by qRT-PCR (see later “Materials and Methods”).

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: .. Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK). ..

Article Title: Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population
Article Snippet: .. Subsequently, libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Short sequence reads were generated using bcl2fastq software (v 1.8.3).

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: .. A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK). ..

Flow Cytometry:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: Single cells were flow-sorted into 96 well plates containing 2μl of phosphate buffered saline. .. Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK).

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: DNA was extracted (DNeasy Blood and Tissue extraction kit, #69506 Qiagen Manchester UK) from bulk and flow-sorted cells from patient samples. .. A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK).

Quantitative RT-PCR:

Article Title: A Short Indel-Lacking-Resistance Gene Triggers Silencing of the Photosynthetic Machinery Components Through TYLCSV-Associated Endogenous siRNAs in Tomato
Article Snippet: Libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Differential expression of selected transcripts was confirmed by qRT-PCR (see later “Materials and Methods”).

Purification:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: .. A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK). ..

Produced:

Article Title: A Short Indel-Lacking-Resistance Gene Triggers Silencing of the Photosynthetic Machinery Components Through TYLCSV-Associated Endogenous siRNAs in Tomato
Article Snippet: .. Libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Differential expression of selected transcripts was confirmed by qRT-PCR (see later “Materials and Methods”).

Article Title: Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population
Article Snippet: .. Subsequently, libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Short sequence reads were generated using bcl2fastq software (v 1.8.3).

High Throughput Screening Assay:

Article Title: A Short Indel-Lacking-Resistance Gene Triggers Silencing of the Photosynthetic Machinery Components Through TYLCSV-Associated Endogenous siRNAs in Tomato
Article Snippet: Libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. To generate miRNA-cleaved target libraries (RNA-end-dataset) from leaf tissue of either mock-inoculated or virus-infected, symptomatic tomato plants we applied the previously described high-throughput experimental approach that identifies mRNAs targeted by miRNAs, also known as “PARE” ( ; ).

Generated:

Article Title: Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population
Article Snippet: Subsequently, libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Short sequence reads were generated using bcl2fastq software (v 1.8.3).

Derivative Assay:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK). .. VAF thresholds for determining detection of mutations were determined by genotyping 48 single cells derived from normal bone marrow, and set at the 95% confidence level (mean ± 1.96 x standard error of mean (SEM); i.e. < 5% chance of false positive, ).

Expressing:

Article Title: A Short Indel-Lacking-Resistance Gene Triggers Silencing of the Photosynthetic Machinery Components Through TYLCSV-Associated Endogenous siRNAs in Tomato
Article Snippet: Libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Differential expression of selected transcripts was confirmed by qRT-PCR (see later “Materials and Methods”).

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK). .. VAF was obtained using the Unix ‘grep’ ( g lobally search re gular expression and p rint) command line.

Lysis:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: Briefly, following cell lysis, alkali denaturation and neutralisation, a master mix containing Phi29 polymerase, dNTPs and random oligonucleotide primers was added. .. Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK).

Polymerase Chain Reaction:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: .. Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK). ..

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: .. A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK). ..

Whole Genome Amplification:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: WGA was carried out at 30°C for 8 hours followed by heat inactivation. .. Barcoding and sequencing oligonucleotides were added by PCR and sequencing performed on Illumina MiSeq (Illumina, Saffron Walden, UK).

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK). .. A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK).

Quantitation Assay:

Article Title: Clonal heterogeneity of acute myeloid leukemia treated with the IDH2 inhibitor Enasidenib
Article Snippet: .. A second PCR reaction added Illumina barcodes and sequencing oligonucleotides prior to sample purification, quantitation, pooling and library preparation for sequencing on Illumina MiSeq (Illumina, Saffron Walden, UK). ..

RNA Extraction:

Article Title: Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population
Article Snippet: Paragraph title: PLANT MATERIALS, VIRUS, RNA EXTRACTION, AND SMALL RNA SEQUENCING ... Subsequently, libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform.

Molecular Weight:

Article Title: Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population
Article Snippet: Low molecular weight RNA was enriched as previously described ( ) and mixed in a ratio of 1000 (chickpea) to 1 (N. benthamiana ) in amount. .. Subsequently, libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform.

Software:

Article Title: Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population
Article Snippet: Subsequently, libraries of sRNAs were produced using a TruSeq Small RNA Sample Kit (Illumina) and sequenced with standard sequencing oligos on the Illumina HiSeq 2500 platform. .. Short sequence reads were generated using bcl2fastq software (v 1.8.3).

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  • 99
    Illumina Inc illumina miniseq sequencer
    Effect of phiX spike-in on observed microbial communities with amplicons generated using ShortEMP primers. Genus-level annotations of sequence data generated on an <t>Illumina</t> <t>MiniSeq</t> instrument were visualized using mMDS ordination employing a distance matrix based on Bray–Curtis similarity. Rarefaction was performed to a depth of 30,000 sequences per sample. For each ShortEMP PCR condition, six technical replicates were analyzed using either 20% or 1.75% phiX spike-in. Small differences, consistent with run-to-run variation, were observed between the two sequencing runs (ANOSIM: 40 °C R = 0.146, p = 0.052; 45 °C R = 0.394, p = 0.011; 50 °C R = 0.178, p = 0.100). Ellipses represent a 95% confidence interval around the centroid.
    Illumina Miniseq Sequencer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miniseq sequencer/product/Illumina Inc
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    illumina miniseq sequencer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Illumina Inc illumina sequencing primer
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Illumina Sequencing Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina sequencing primer/product/Illumina Inc
    Average 99 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    illumina sequencing primer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Illumina Inc 16s rrna gene
    Hh infection induces IL-22 dependent dysbiosis (A) Quantification of Hh colonization density in the cecal content. n=9-11. (B) Chao and Shannon diversity indices from <t>16S</t> <t>rRNA</t> sequencing data obtained from fecal pellets of uninfected mice and Hh infected mice treated with control or anti-IL-22 antibody as indicated. (C) Relative abundance of indicated taxa from groups described in B. (D) Relative abundance of indicated taxa as determined by taxa specific 16s rRNA qPCR from groups described in B.
    16s Rrna Gene, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene/product/Illumina Inc
    Average 99 stars, based on 1085 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    92
    Illumina Inc illumina dna microarray
    Fold change (FC) values of upregulated ( red ) and downregulated ( green ) genes in dexmedetomidine-treated (100 nM) A7r5-α 2B vascular smooth muscle cells compared with vehicle-treated control cells in the <t>Illumina</t> <t>DNA</t> <t>microarray.</t> The genes are ordered based on the associated adjusted p- values, in decreasing order. Explanation of gene symbols, actual and adjusted p- values can be found in Additional file 2
    Illumina Dna Microarray, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina dna microarray/product/Illumina Inc
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    illumina dna microarray - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effect of phiX spike-in on observed microbial communities with amplicons generated using ShortEMP primers. Genus-level annotations of sequence data generated on an Illumina MiniSeq instrument were visualized using mMDS ordination employing a distance matrix based on Bray–Curtis similarity. Rarefaction was performed to a depth of 30,000 sequences per sample. For each ShortEMP PCR condition, six technical replicates were analyzed using either 20% or 1.75% phiX spike-in. Small differences, consistent with run-to-run variation, were observed between the two sequencing runs (ANOSIM: 40 °C R = 0.146, p = 0.052; 45 °C R = 0.394, p = 0.011; 50 °C R = 0.178, p = 0.100). Ellipses represent a 95% confidence interval around the centroid.

    Journal: PeerJ

    Article Title: PCR effects of melting temperature adjustment of individual primers in degenerate primer pools

    doi: 10.7717/peerj.6570

    Figure Lengend Snippet: Effect of phiX spike-in on observed microbial communities with amplicons generated using ShortEMP primers. Genus-level annotations of sequence data generated on an Illumina MiniSeq instrument were visualized using mMDS ordination employing a distance matrix based on Bray–Curtis similarity. Rarefaction was performed to a depth of 30,000 sequences per sample. For each ShortEMP PCR condition, six technical replicates were analyzed using either 20% or 1.75% phiX spike-in. Small differences, consistent with run-to-run variation, were observed between the two sequencing runs (ANOSIM: 40 °C R = 0.146, p = 0.052; 45 °C R = 0.394, p = 0.011; 50 °C R = 0.178, p = 0.100). Ellipses represent a 95% confidence interval around the centroid.

    Article Snippet: When amplicons generated with the modified primers were sequenced on an Illumina MiniSeq sequencer with < 2% phiX, the data quality and community analyses were consistent with the same amplicons generated on a run with 20% phiX.

    Techniques: Generated, Sequencing, Polymerase Chain Reaction

    Effect of annealing temperature and primer set on observed microbial community in Lake Michigan sediment. A composite sample of gDNA from Lake Michigan sediment was amplified using EMP or ShortEMP primer pools at annealing temperatures of 40, 45 and 50 °C and sequenced on an Illumina MiniSeq instrument. Sequence data were rarefied to a depth of 17,500 sequences per sample. (A) Genus-level annotations of sequence data were visualized using metric multidimensional scaling (mMDS) employing a distance matrix based on Bray–Curtis similarity. Across all temperatures, the use of EMP and ShortEMP primers resulted in slightly, but significantly, different observed microbial communities (ANOSIM R = 0.473; p

    Journal: PeerJ

    Article Title: PCR effects of melting temperature adjustment of individual primers in degenerate primer pools

    doi: 10.7717/peerj.6570

    Figure Lengend Snippet: Effect of annealing temperature and primer set on observed microbial community in Lake Michigan sediment. A composite sample of gDNA from Lake Michigan sediment was amplified using EMP or ShortEMP primer pools at annealing temperatures of 40, 45 and 50 °C and sequenced on an Illumina MiniSeq instrument. Sequence data were rarefied to a depth of 17,500 sequences per sample. (A) Genus-level annotations of sequence data were visualized using metric multidimensional scaling (mMDS) employing a distance matrix based on Bray–Curtis similarity. Across all temperatures, the use of EMP and ShortEMP primers resulted in slightly, but significantly, different observed microbial communities (ANOSIM R = 0.473; p

    Article Snippet: When amplicons generated with the modified primers were sequenced on an Illumina MiniSeq sequencer with < 2% phiX, the data quality and community analyses were consistent with the same amplicons generated on a run with 20% phiX.

    Techniques: Amplification, Sequencing

    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the Illumina sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.

    Journal: PLoS Genetics

    Article Title: The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

    doi: 10.1371/journal.pgen.1003834

    Figure Lengend Snippet: Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the Illumina sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.

    Article Snippet: This oligonucleotide incorporates the Illumina sequencing primer-binding site into transposon specific DNA fragments, enabling the use of the standard Illumina sequencing primer and eliminating the need to design and optimize another sequencing primer for each new transposon sequence.

    Techniques: Selection, Sequencing

    Hh infection induces IL-22 dependent dysbiosis (A) Quantification of Hh colonization density in the cecal content. n=9-11. (B) Chao and Shannon diversity indices from 16S rRNA sequencing data obtained from fecal pellets of uninfected mice and Hh infected mice treated with control or anti-IL-22 antibody as indicated. (C) Relative abundance of indicated taxa from groups described in B. (D) Relative abundance of indicated taxa as determined by taxa specific 16s rRNA qPCR from groups described in B.

    Journal: Mucosal immunology

    Article Title: Interleukin-22 drives nitric oxide-dependent DNA damage and dysplasia in a murine model of colitis-associated cancer

    doi: 10.1038/mi.2017.9

    Figure Lengend Snippet: Hh infection induces IL-22 dependent dysbiosis (A) Quantification of Hh colonization density in the cecal content. n=9-11. (B) Chao and Shannon diversity indices from 16S rRNA sequencing data obtained from fecal pellets of uninfected mice and Hh infected mice treated with control or anti-IL-22 antibody as indicated. (C) Relative abundance of indicated taxa from groups described in B. (D) Relative abundance of indicated taxa as determined by taxa specific 16s rRNA qPCR from groups described in B.

    Article Snippet: Amplicons were generated using oligonucleotide primers that target approximately 300 bp of the V4 variable region of the 16S rRNA gene (primers 515F and 806R) and also were barcoded and pooled to construct the sequencing library, followed by sequencing with an Illumina MiSeq instrument to generate paired-end 150×150 reads.

    Techniques: Infection, Sequencing, Mouse Assay, Real-time Polymerase Chain Reaction

    Fold change (FC) values of upregulated ( red ) and downregulated ( green ) genes in dexmedetomidine-treated (100 nM) A7r5-α 2B vascular smooth muscle cells compared with vehicle-treated control cells in the Illumina DNA microarray. The genes are ordered based on the associated adjusted p- values, in decreasing order. Explanation of gene symbols, actual and adjusted p- values can be found in Additional file 2

    Journal: BMC Systems Biology

    Article Title: Gene expression profiles and signaling mechanisms in α2B-adrenoceptor-evoked proliferation of vascular smooth muscle cells

    doi: 10.1186/s12918-017-0439-8

    Figure Lengend Snippet: Fold change (FC) values of upregulated ( red ) and downregulated ( green ) genes in dexmedetomidine-treated (100 nM) A7r5-α 2B vascular smooth muscle cells compared with vehicle-treated control cells in the Illumina DNA microarray. The genes are ordered based on the associated adjusted p- values, in decreasing order. Explanation of gene symbols, actual and adjusted p- values can be found in Additional file 2

    Article Snippet: Illumina DNA microarray The objective of this set of experiments was to identify specific genes involved in α2B -adrenoceptor-evoked VSMC proliferation.

    Techniques: Microarray