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Illumina Inc sequencing methods rnase l
Viral RNA fragments produced by <t>RNase</t> L and RNase A. HCV and PV RNAs were incubated with RNase L and RNase A to produce RNA fragments for 2′, 3′-cyclic phosphate cDNA synthesis and sequencing. Agarose gel electrophoresis and ethidium bromide staining revealed the size of viral RNA fragments. ( A ) Diagram of HCV and PV RNAs. HCV RNA is 9648 bases long. PV RNA is 7500 bases long. ( B ) Viral RNAs incubated with RNase L. HCV and PV RNAs were incubated with RNase L for 20 min in the absence of 2-5A (no 2-5A), or with RNase L and 2-5A for 0, 2.5, 5, 10 and 20 min. ( C ) Viral RNAs incubated with RNase A. HCV and PV RNAs were incubated for 20 min in the absence of RNase A (−), and the presence of RNase A for 0, 2.5, 5, 10 and 20 min.
Sequencing Methods Rnase L, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequencing methods rnase l/product/Illumina Inc
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sequencing methods rnase l - by Bioz Stars, 2020-04
85/100 stars

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Article Title: Ribonuclease L and metal-ion-independent endoribonuclease cleavage sites in host and viral RNAs

Journal: Nucleic Acids Research

doi: 10.1093/nar/gku118

Viral RNA fragments produced by RNase L and RNase A. HCV and PV RNAs were incubated with RNase L and RNase A to produce RNA fragments for 2′, 3′-cyclic phosphate cDNA synthesis and sequencing. Agarose gel electrophoresis and ethidium bromide staining revealed the size of viral RNA fragments. ( A ) Diagram of HCV and PV RNAs. HCV RNA is 9648 bases long. PV RNA is 7500 bases long. ( B ) Viral RNAs incubated with RNase L. HCV and PV RNAs were incubated with RNase L for 20 min in the absence of 2-5A (no 2-5A), or with RNase L and 2-5A for 0, 2.5, 5, 10 and 20 min. ( C ) Viral RNAs incubated with RNase A. HCV and PV RNAs were incubated for 20 min in the absence of RNase A (−), and the presence of RNase A for 0, 2.5, 5, 10 and 20 min.
Figure Legend Snippet: Viral RNA fragments produced by RNase L and RNase A. HCV and PV RNAs were incubated with RNase L and RNase A to produce RNA fragments for 2′, 3′-cyclic phosphate cDNA synthesis and sequencing. Agarose gel electrophoresis and ethidium bromide staining revealed the size of viral RNA fragments. ( A ) Diagram of HCV and PV RNAs. HCV RNA is 9648 bases long. PV RNA is 7500 bases long. ( B ) Viral RNAs incubated with RNase L. HCV and PV RNAs were incubated with RNase L for 20 min in the absence of 2-5A (no 2-5A), or with RNase L and 2-5A for 0, 2.5, 5, 10 and 20 min. ( C ) Viral RNAs incubated with RNase A. HCV and PV RNAs were incubated for 20 min in the absence of RNase A (−), and the presence of RNase A for 0, 2.5, 5, 10 and 20 min.

Techniques Used: Produced, Incubation, Sequencing, Agarose Gel Electrophoresis, Staining

Cleavage sites mapped onto rRNA secondary and tertiary structures. Secondary and tertiary structures from Anger et al. ( 42 ). ( A ) RNase L cleavage sites in 18S rRNA secondary structure. Portion of 18S rRNA structure highlighting the location of RNase L cleavage sites. ( B ) Location of RNase L cleavage sites in 80S ribosome tertiary structure. RNase L-dependent cleavage sites highlighted in red spheres. ( C ) 3′-end of 5.8S and 5S rRNAs. 3′-end of 5.8S and 5S rRNAs highlighted in orange spheres. ( D ) RNase L-independent cleavage sites. Some representative RNase L-independent cleavage sites highlighted in yellow spheres.
Figure Legend Snippet: Cleavage sites mapped onto rRNA secondary and tertiary structures. Secondary and tertiary structures from Anger et al. ( 42 ). ( A ) RNase L cleavage sites in 18S rRNA secondary structure. Portion of 18S rRNA structure highlighting the location of RNase L cleavage sites. ( B ) Location of RNase L cleavage sites in 80S ribosome tertiary structure. RNase L-dependent cleavage sites highlighted in red spheres. ( C ) 3′-end of 5.8S and 5S rRNAs. 3′-end of 5.8S and 5S rRNAs highlighted in orange spheres. ( D ) RNase L-independent cleavage sites. Some representative RNase L-independent cleavage sites highlighted in yellow spheres.

Techniques Used:

Endoribonuclease cleavage sites in rRNAs from W12 HeLa cells. RNAs from mock-infected and PV-infected W12 HeLa cells ( Figure 3 B) were used for 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing. The location and frequency of cleavage sites in 28S rRNA ( A ), 18S rRNA ( B ), 5.8S rRNA ( C ) and 5S rRNA ( D ) are shown for mock-infected and PV-infected RNA samples isolated at 8 hpa. X-axis: Nucleotide position of each RNA. Y-axis: Percentage of total UMIs at each cleavage site. Dinucleotides at the 3′-end of abundant RNA fragments are annotated at the corresponding positions in the graphs. The locations of GC-rich expansion segments are highlighted by light blue rectangles. RNase L cleavage sites are highlighted in red.
Figure Legend Snippet: Endoribonuclease cleavage sites in rRNAs from W12 HeLa cells. RNAs from mock-infected and PV-infected W12 HeLa cells ( Figure 3 B) were used for 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing. The location and frequency of cleavage sites in 28S rRNA ( A ), 18S rRNA ( B ), 5.8S rRNA ( C ) and 5S rRNA ( D ) are shown for mock-infected and PV-infected RNA samples isolated at 8 hpa. X-axis: Nucleotide position of each RNA. Y-axis: Percentage of total UMIs at each cleavage site. Dinucleotides at the 3′-end of abundant RNA fragments are annotated at the corresponding positions in the graphs. The locations of GC-rich expansion segments are highlighted by light blue rectangles. RNase L cleavage sites are highlighted in red.

Techniques Used: Infection, Sequencing, Isolation

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Article Title: Ribonuclease L and metal-ion-independent endoribonuclease cleavage sites in host and viral RNAs
Article Snippet: .. 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing methods RNase L, RNase A and other metal-ion–independent endoribonucleases target single-stranded regions of RNA, leaving 2′, 3′-cyclic phosphates at the end of RNA fragments ( ). .. We exploited the 2′, 3′-cyclic phosphates at RNA cleavage sites to make cDNA libraries suitable for Illumina sequencing ( Supplementary Figure S1 ).

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    Illumina Inc sequencing methods rnase l
    Viral RNA fragments produced by <t>RNase</t> L and RNase A. HCV and PV RNAs were incubated with RNase L and RNase A to produce RNA fragments for 2′, 3′-cyclic phosphate cDNA synthesis and sequencing. Agarose gel electrophoresis and ethidium bromide staining revealed the size of viral RNA fragments. ( A ) Diagram of HCV and PV RNAs. HCV RNA is 9648 bases long. PV RNA is 7500 bases long. ( B ) Viral RNAs incubated with RNase L. HCV and PV RNAs were incubated with RNase L for 20 min in the absence of 2-5A (no 2-5A), or with RNase L and 2-5A for 0, 2.5, 5, 10 and 20 min. ( C ) Viral RNAs incubated with RNase A. HCV and PV RNAs were incubated for 20 min in the absence of RNase A (−), and the presence of RNase A for 0, 2.5, 5, 10 and 20 min.
    Sequencing Methods Rnase L, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing methods rnase l/product/Illumina Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequencing methods rnase l - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

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    Viral RNA fragments produced by RNase L and RNase A. HCV and PV RNAs were incubated with RNase L and RNase A to produce RNA fragments for 2′, 3′-cyclic phosphate cDNA synthesis and sequencing. Agarose gel electrophoresis and ethidium bromide staining revealed the size of viral RNA fragments. ( A ) Diagram of HCV and PV RNAs. HCV RNA is 9648 bases long. PV RNA is 7500 bases long. ( B ) Viral RNAs incubated with RNase L. HCV and PV RNAs were incubated with RNase L for 20 min in the absence of 2-5A (no 2-5A), or with RNase L and 2-5A for 0, 2.5, 5, 10 and 20 min. ( C ) Viral RNAs incubated with RNase A. HCV and PV RNAs were incubated for 20 min in the absence of RNase A (−), and the presence of RNase A for 0, 2.5, 5, 10 and 20 min.

    Journal: Nucleic Acids Research

    Article Title: Ribonuclease L and metal-ion-independent endoribonuclease cleavage sites in host and viral RNAs

    doi: 10.1093/nar/gku118

    Figure Lengend Snippet: Viral RNA fragments produced by RNase L and RNase A. HCV and PV RNAs were incubated with RNase L and RNase A to produce RNA fragments for 2′, 3′-cyclic phosphate cDNA synthesis and sequencing. Agarose gel electrophoresis and ethidium bromide staining revealed the size of viral RNA fragments. ( A ) Diagram of HCV and PV RNAs. HCV RNA is 9648 bases long. PV RNA is 7500 bases long. ( B ) Viral RNAs incubated with RNase L. HCV and PV RNAs were incubated with RNase L for 20 min in the absence of 2-5A (no 2-5A), or with RNase L and 2-5A for 0, 2.5, 5, 10 and 20 min. ( C ) Viral RNAs incubated with RNase A. HCV and PV RNAs were incubated for 20 min in the absence of RNase A (−), and the presence of RNase A for 0, 2.5, 5, 10 and 20 min.

    Article Snippet: 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing methods RNase L, RNase A and other metal-ion–independent endoribonucleases target single-stranded regions of RNA, leaving 2′, 3′-cyclic phosphates at the end of RNA fragments ( ).

    Techniques: Produced, Incubation, Sequencing, Agarose Gel Electrophoresis, Staining

    Cleavage sites mapped onto rRNA secondary and tertiary structures. Secondary and tertiary structures from Anger et al. ( 42 ). ( A ) RNase L cleavage sites in 18S rRNA secondary structure. Portion of 18S rRNA structure highlighting the location of RNase L cleavage sites. ( B ) Location of RNase L cleavage sites in 80S ribosome tertiary structure. RNase L-dependent cleavage sites highlighted in red spheres. ( C ) 3′-end of 5.8S and 5S rRNAs. 3′-end of 5.8S and 5S rRNAs highlighted in orange spheres. ( D ) RNase L-independent cleavage sites. Some representative RNase L-independent cleavage sites highlighted in yellow spheres.

    Journal: Nucleic Acids Research

    Article Title: Ribonuclease L and metal-ion-independent endoribonuclease cleavage sites in host and viral RNAs

    doi: 10.1093/nar/gku118

    Figure Lengend Snippet: Cleavage sites mapped onto rRNA secondary and tertiary structures. Secondary and tertiary structures from Anger et al. ( 42 ). ( A ) RNase L cleavage sites in 18S rRNA secondary structure. Portion of 18S rRNA structure highlighting the location of RNase L cleavage sites. ( B ) Location of RNase L cleavage sites in 80S ribosome tertiary structure. RNase L-dependent cleavage sites highlighted in red spheres. ( C ) 3′-end of 5.8S and 5S rRNAs. 3′-end of 5.8S and 5S rRNAs highlighted in orange spheres. ( D ) RNase L-independent cleavage sites. Some representative RNase L-independent cleavage sites highlighted in yellow spheres.

    Article Snippet: 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing methods RNase L, RNase A and other metal-ion–independent endoribonucleases target single-stranded regions of RNA, leaving 2′, 3′-cyclic phosphates at the end of RNA fragments ( ).

    Techniques:

    Endoribonuclease cleavage sites in rRNAs from W12 HeLa cells. RNAs from mock-infected and PV-infected W12 HeLa cells ( Figure 3 B) were used for 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing. The location and frequency of cleavage sites in 28S rRNA ( A ), 18S rRNA ( B ), 5.8S rRNA ( C ) and 5S rRNA ( D ) are shown for mock-infected and PV-infected RNA samples isolated at 8 hpa. X-axis: Nucleotide position of each RNA. Y-axis: Percentage of total UMIs at each cleavage site. Dinucleotides at the 3′-end of abundant RNA fragments are annotated at the corresponding positions in the graphs. The locations of GC-rich expansion segments are highlighted by light blue rectangles. RNase L cleavage sites are highlighted in red.

    Journal: Nucleic Acids Research

    Article Title: Ribonuclease L and metal-ion-independent endoribonuclease cleavage sites in host and viral RNAs

    doi: 10.1093/nar/gku118

    Figure Lengend Snippet: Endoribonuclease cleavage sites in rRNAs from W12 HeLa cells. RNAs from mock-infected and PV-infected W12 HeLa cells ( Figure 3 B) were used for 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing. The location and frequency of cleavage sites in 28S rRNA ( A ), 18S rRNA ( B ), 5.8S rRNA ( C ) and 5S rRNA ( D ) are shown for mock-infected and PV-infected RNA samples isolated at 8 hpa. X-axis: Nucleotide position of each RNA. Y-axis: Percentage of total UMIs at each cleavage site. Dinucleotides at the 3′-end of abundant RNA fragments are annotated at the corresponding positions in the graphs. The locations of GC-rich expansion segments are highlighted by light blue rectangles. RNase L cleavage sites are highlighted in red.

    Article Snippet: 2′, 3′-cyclic phosphate cDNA synthesis and Illumina sequencing methods RNase L, RNase A and other metal-ion–independent endoribonucleases target single-stranded regions of RNA, leaving 2′, 3′-cyclic phosphates at the end of RNA fragments ( ).

    Techniques: Infection, Sequencing, Isolation