sequencing long range polymerase chain reaction pcr  (Illumina Inc)

 
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    Illumina Inc sequencing long range polymerase chain reaction pcr
    Sequencing Long Range Polymerase Chain Reaction Pcr, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing long range polymerase chain reaction pcr/product/Illumina Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequencing long range polymerase chain reaction pcr - by Bioz Stars, 2020-09
    85/100 stars

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    Sequencing:

    Article Title: Index-Free De Novo Assembly and Deconvolution of Mixed Mitochondrial Genomes
    Article Snippet: .. Sequencing Long-range polymerase chain reaction (PCR) products were generated from a diverse set of templates in order to create a mixture of templates to sequence using an Illumina GA. ..

    Polymerase Chain Reaction:

    Article Title: Index-Free De Novo Assembly and Deconvolution of Mixed Mitochondrial Genomes
    Article Snippet: .. Sequencing Long-range polymerase chain reaction (PCR) products were generated from a diverse set of templates in order to create a mixture of templates to sequence using an Illumina GA. ..

    Generated:

    Article Title: Index-Free De Novo Assembly and Deconvolution of Mixed Mitochondrial Genomes
    Article Snippet: .. Sequencing Long-range polymerase chain reaction (PCR) products were generated from a diverse set of templates in order to create a mixture of templates to sequence using an Illumina GA. ..

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    Illumina Inc range pcr
    PCIP-seq applied to ATL (a) In ATL100 both <t>Illumina</t> and Nanopore based methods show a single predominant insertion site (b) Screen shot from IGV shows a ∼16kb window with the provirus insertion site in the tumor clone identified via PCIP-seq and ligation mediated <t>PCR</t> with Illumina sequencing (c) PCIP-seq reads in IGV show a ∼3,600bp deletion in the provirus, confirmed via long range PCR and Illumina sequencing. (d) The ATL2 tumor clone contains three proviruses (named according to chromosome inserted into), the provirus on chr1 inserted into a repetitive element (LTR) and short reads generated from host DNA flanking the insertion site map to multiple positions in the genome. Filtering out multi-mapping reads causes an underestimation of the abundance of this insertion site (13.6 %), this can be partially corrected by retaining multi-mapping reads at this position (25.4 %). However, that approach can cause the potentially spurious inflation of other integration sites (red slice 9%). The long PCIP-seq reads can span repetitive elements and produce even coverage for each provirus without correction. (e) Screen shot from IGV shows representative reads coming from the three proviruses at positions where four de novo mutations were observed.
    Range Pcr, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/range pcr/product/Illumina Inc
    Average 89 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    range pcr - by Bioz Stars, 2020-09
    89/100 stars
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    PCIP-seq applied to ATL (a) In ATL100 both Illumina and Nanopore based methods show a single predominant insertion site (b) Screen shot from IGV shows a ∼16kb window with the provirus insertion site in the tumor clone identified via PCIP-seq and ligation mediated PCR with Illumina sequencing (c) PCIP-seq reads in IGV show a ∼3,600bp deletion in the provirus, confirmed via long range PCR and Illumina sequencing. (d) The ATL2 tumor clone contains three proviruses (named according to chromosome inserted into), the provirus on chr1 inserted into a repetitive element (LTR) and short reads generated from host DNA flanking the insertion site map to multiple positions in the genome. Filtering out multi-mapping reads causes an underestimation of the abundance of this insertion site (13.6 %), this can be partially corrected by retaining multi-mapping reads at this position (25.4 %). However, that approach can cause the potentially spurious inflation of other integration sites (red slice 9%). The long PCIP-seq reads can span repetitive elements and produce even coverage for each provirus without correction. (e) Screen shot from IGV shows representative reads coming from the three proviruses at positions where four de novo mutations were observed.

    Journal: bioRxiv

    Article Title: Pooled CRISPR Inverse PCR sequencing (PCIP-seq): simultaneous sequencing of retroviral insertion points and the integrated provirus with long reads

    doi: 10.1101/558130

    Figure Lengend Snippet: PCIP-seq applied to ATL (a) In ATL100 both Illumina and Nanopore based methods show a single predominant insertion site (b) Screen shot from IGV shows a ∼16kb window with the provirus insertion site in the tumor clone identified via PCIP-seq and ligation mediated PCR with Illumina sequencing (c) PCIP-seq reads in IGV show a ∼3,600bp deletion in the provirus, confirmed via long range PCR and Illumina sequencing. (d) The ATL2 tumor clone contains three proviruses (named according to chromosome inserted into), the provirus on chr1 inserted into a repetitive element (LTR) and short reads generated from host DNA flanking the insertion site map to multiple positions in the genome. Filtering out multi-mapping reads causes an underestimation of the abundance of this insertion site (13.6 %), this can be partially corrected by retaining multi-mapping reads at this position (25.4 %). However, that approach can cause the potentially spurious inflation of other integration sites (red slice 9%). The long PCIP-seq reads can span repetitive elements and produce even coverage for each provirus without correction. (e) Screen shot from IGV shows representative reads coming from the three proviruses at positions where four de novo mutations were observed.

    Article Snippet: We validated three ERVs via long range PCR and Illumina sequencing (Supplementary Fig. 10).

    Techniques: Ligation, Polymerase Chain Reaction, Sequencing, Generated