Structured Review

Illumina Inc sequencing adapter oligos
Sequencing Adapter Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequencing adapter oligos/product/Illumina Inc
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sequencing adapter oligos - by Bioz Stars, 2020-04
94/100 stars

Images

Related Articles

Selection:

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA). .. Size selection was performed by agarose gel electrophoresis, generating cDNA libraries ranging from 200–250 bp.

Agarose Gel Electrophoresis:

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA). .. Size selection was performed by agarose gel electrophoresis, generating cDNA libraries ranging from 200–250 bp.

Synthesized:

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: First strand cDNA was synthesized using random hexamers, followed by double-stranded cDNA synthesis in a reaction containing buffer solution, dNTPs, RNase H, and DNA polymerase I. .. The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA).

Isolation:

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: Briefly, poly(A) mRNA was isolated from total RNA samples using magnetic oligo(dT) beads and subjected to fragmentation. .. The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA).

Purification:

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: .. The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA). .. Size selection was performed by agarose gel electrophoresis, generating cDNA libraries ranging from 200–250 bp.

Sequencing:

Article Title: Differential regulation of OCT4 targets facilitates reacquisition of pluripotency
Article Snippet: .. In brief, immunoprecipitated DNA was end repaired using the End-IT DNA End-Repair Kit (Epicentre), extended using Klenow fragment (3’−5’ exo) (NEB), and ligated to sequencing adapter oligos (Illumina). .. Each library was PCR-amplified using PFU Ultra II Hotstart Master Mix (Agilent), and a size range of 300–600 base pairs selected for sequencing.

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: .. The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA). .. Size selection was performed by agarose gel electrophoresis, generating cDNA libraries ranging from 200–250 bp.

Immunoprecipitation:

Article Title: Differential regulation of OCT4 targets facilitates reacquisition of pluripotency
Article Snippet: .. In brief, immunoprecipitated DNA was end repaired using the End-IT DNA End-Repair Kit (Epicentre), extended using Klenow fragment (3’−5’ exo) (NEB), and ligated to sequencing adapter oligos (Illumina). .. Each library was PCR-amplified using PFU Ultra II Hotstart Master Mix (Agilent), and a size range of 300–600 base pairs selected for sequencing.

Incubation:

Article Title: Differential regulation of OCT4 targets facilitates reacquisition of pluripotency
Article Snippet: Chromatin was incubated with antibody at 4 °C overnight with constant rotation. .. In brief, immunoprecipitated DNA was end repaired using the End-IT DNA End-Repair Kit (Epicentre), extended using Klenow fragment (3’−5’ exo) (NEB), and ligated to sequencing adapter oligos (Illumina).

Amplification:

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA). .. After PCR amplification, the mRNA library was sequenced using HiSeq 2000 (Illumina Inc.).

Polymerase Chain Reaction:

Article Title: Differential regulation of OCT4 targets facilitates reacquisition of pluripotency
Article Snippet: In brief, immunoprecipitated DNA was end repaired using the End-IT DNA End-Repair Kit (Epicentre), extended using Klenow fragment (3’−5’ exo) (NEB), and ligated to sequencing adapter oligos (Illumina). .. Each library was PCR-amplified using PFU Ultra II Hotstart Master Mix (Agilent), and a size range of 300–600 base pairs selected for sequencing.

Article Title: Identification of a Core miRNA-Pathway Regulatory Network in Glioma by Therapeutically Targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3
Article Snippet: .. The cDNA was purified using the QiaQuick PCR purification kit (Qiagen) and resuspended in elution buffer, and after end repair and addition of a single A base, cDNA fragments were ligated to sequencing adapter oligos (Illumina Inc., San Diego, CA, USA). .. Size selection was performed by agarose gel electrophoresis, generating cDNA libraries ranging from 200–250 bp.

Chromatin Immunoprecipitation:

Article Title: Differential regulation of OCT4 targets facilitates reacquisition of pluripotency
Article Snippet: Paragraph title: ChIP and ChIP-seq library construction ... In brief, immunoprecipitated DNA was end repaired using the End-IT DNA End-Repair Kit (Epicentre), extended using Klenow fragment (3’−5’ exo) (NEB), and ligated to sequencing adapter oligos (Illumina).

ChIP-sequencing:

Article Title: Differential regulation of OCT4 targets facilitates reacquisition of pluripotency
Article Snippet: Paragraph title: ChIP and ChIP-seq library construction ... In brief, immunoprecipitated DNA was end repaired using the End-IT DNA End-Repair Kit (Epicentre), extended using Klenow fragment (3’−5’ exo) (NEB), and ligated to sequencing adapter oligos (Illumina).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Illumina Inc p5 p7 primers sequence
    Schematic of the ddPCR assay to test the amount of DNA remaining at each step of the library preparation (using DNA fragment specific primers shown by blue arrows [ 15 ]) and to measure the amount of DNA fragment bearing adaptors after the ligation step (using the adaptor specific primers shown in orange ) and <t>P5/P7</t> primers after the amplification step (using the P5/P7 primers shown in green )
    P5 P7 Primers Sequence, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p5 p7 primers sequence/product/Illumina Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p5 p7 primers sequence - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    99
    Illumina Inc truseq adapter sequences
    Schematic of the ddPCR assay to test the amount of DNA remaining at each step of the library preparation (using DNA fragment specific primers shown by blue arrows [ 15 ]) and to measure the amount of DNA fragment bearing adaptors after the ligation step (using the adaptor specific primers shown in orange ) and <t>P5/P7</t> primers after the amplification step (using the P5/P7 primers shown in green )
    Truseq Adapter Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq adapter sequences/product/Illumina Inc
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    truseq adapter sequences - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Illumina Inc primer
    Schematic of the ddPCR assay to test the amount of DNA remaining at each step of the library preparation (using DNA fragment specific primers shown by blue arrows [ 15 ]) and to measure the amount of DNA fragment bearing adaptors after the ligation step (using the adaptor specific primers shown in orange ) and <t>P5/P7</t> primers after the amplification step (using the P5/P7 primers shown in green )
    Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer/product/Illumina Inc
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    primer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    Illumina Inc page purified illumina platform compatible adaptor oligonucleotides
    Schematic of the ddPCR assay to test the amount of DNA remaining at each step of the library preparation (using DNA fragment specific primers shown by blue arrows [ 15 ]) and to measure the amount of DNA fragment bearing adaptors after the ligation step (using the adaptor specific primers shown in orange ) and <t>P5/P7</t> primers after the amplification step (using the P5/P7 primers shown in green )
    Page Purified Illumina Platform Compatible Adaptor Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/page purified illumina platform compatible adaptor oligonucleotides/product/Illumina Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    page purified illumina platform compatible adaptor oligonucleotides - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Schematic of the ddPCR assay to test the amount of DNA remaining at each step of the library preparation (using DNA fragment specific primers shown by blue arrows [ 15 ]) and to measure the amount of DNA fragment bearing adaptors after the ligation step (using the adaptor specific primers shown in orange ) and P5/P7 primers after the amplification step (using the P5/P7 primers shown in green )

    Journal: BMC Genomics

    Article Title: Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

    doi: 10.1186/s12864-016-2757-4

    Figure Lengend Snippet: Schematic of the ddPCR assay to test the amount of DNA remaining at each step of the library preparation (using DNA fragment specific primers shown by blue arrows [ 15 ]) and to measure the amount of DNA fragment bearing adaptors after the ligation step (using the adaptor specific primers shown in orange ) and P5/P7 primers after the amplification step (using the P5/P7 primers shown in green )

    Article Snippet: It is important to highlight that not only the Truseq DNA PCR-free kit but also any other kit exhibiting a high ligation efficiency could potentially be used without any PCR step, as long as the sequence of the used adaptors contains the P5/P7 primers sequence necessary for sequencing on an Illumina platform.

    Techniques: Ligation, Amplification

    Example of yield measurements obtained with the NEBNext kit when comparing the amount of DNA at each step versus the initial DNA input (500 ng). Blue bars correspond to values measured using the PhiX specific primers and reflect the DNA loss at each step mainly due to pipetting and bead clean up. The orange bar corresponds to the value measured with the adaptor specific primers and reflects the amount of DNA in the sample bearing adaptor after the ligation step in comparison with the initial DNA input. The green bar corresponds to the value measured with the P5/P7 primers and reflects the amount of sequencable DNA in the sample at the end of the library preparation

    Journal: BMC Genomics

    Article Title: Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

    doi: 10.1186/s12864-016-2757-4

    Figure Lengend Snippet: Example of yield measurements obtained with the NEBNext kit when comparing the amount of DNA at each step versus the initial DNA input (500 ng). Blue bars correspond to values measured using the PhiX specific primers and reflect the DNA loss at each step mainly due to pipetting and bead clean up. The orange bar corresponds to the value measured with the adaptor specific primers and reflects the amount of DNA in the sample bearing adaptor after the ligation step in comparison with the initial DNA input. The green bar corresponds to the value measured with the P5/P7 primers and reflects the amount of sequencable DNA in the sample at the end of the library preparation

    Article Snippet: It is important to highlight that not only the Truseq DNA PCR-free kit but also any other kit exhibiting a high ligation efficiency could potentially be used without any PCR step, as long as the sequence of the used adaptors contains the P5/P7 primers sequence necessary for sequencing on an Illumina platform.

    Techniques: Ligation