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Conserved regions of the 5ʹ untranslated region of <t>vafklB</t> , and selected predicted promoter regions. ( a ) <t>Comparative</t> <t>sequence</t> analysis of the untranslated region from V. anguillarum NB10 in the 5ʹ direction of vafklB with the untranslated regions corresponding to vafklB from related species of the genus Vibrio . ( b ) Design of stepwise deleted recombinant promoter regions to identify the core promoter regions. The color shading represents the matching nucleotides among the different Vibrio species. P1 to P6 represent the selected promoter regions.
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Conserved regions of the 5ʹ untranslated region of vafklB , and selected predicted promoter regions. ( a ) Comparative sequence analysis of the untranslated region from V. anguillarum NB10 in the 5ʹ direction of vafklB with the untranslated regions corresponding to vafklB from related species of the genus Vibrio . ( b ) Design of stepwise deleted recombinant promoter regions to identify the core promoter regions. The color shading represents the matching nucleotides among the different Vibrio species. P1 to P6 represent the selected promoter regions.

Journal: Scientific Reports

Article Title: Genetic characteristics of novel extreme alkaline-inducible promoter located in five prime upstream region of peptidyl-prolyl cis/trans isomerase from Vibrio anguillarum

doi: 10.1038/s41598-025-98559-y

Figure Lengend Snippet: Conserved regions of the 5ʹ untranslated region of vafklB , and selected predicted promoter regions. ( a ) Comparative sequence analysis of the untranslated region from V. anguillarum NB10 in the 5ʹ direction of vafklB with the untranslated regions corresponding to vafklB from related species of the genus Vibrio . ( b ) Design of stepwise deleted recombinant promoter regions to identify the core promoter regions. The color shading represents the matching nucleotides among the different Vibrio species. P1 to P6 represent the selected promoter regions.

Article Snippet: VaFklB exhibited amino acid sequence similarities of 91.7%, 92.2%, 87.8%, and 89.8% with the peptidylprolyl isomerases (protein ID: AMG05761.1 ) from V . parahaemolyticus ATCC 17,802, peptidylprolyl isomerase ( AIV04382.1 ) from V . harveyi ATCC 33,843, FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase ( AGV18288.1 ) from V . alginolyticus ATCC 17,749, and the FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase ( WDY52166.1 ) from V . fluvialis 10 M-VF, respectively.

Techniques: Sequencing, Recombinant