Sequant Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum"
Article Title: Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum
Journal: Frontiers in Microbiology
Figure Legend Snippet: In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
Techniques Used: In Vitro, Activity Assay, High Performance Liquid Chromatography, Concentration Assay, Mass Spectrometry
Article Title: Continuous Culture Adaptation of Methylobacterium extorquens AM1 and TK 0001 to Very High Methanol Concentrations
Article Snippet: Lactate detection was performed on a Thermo Fisher ScientificTM DionexTM UltiMateTM 3000 Rapid Separation (3000RS) liquid chromatography (LC) system (Thermo Fisher Scientific, Courtaboeuf, France) coupled to ultra-high resolution Orbitrap Elite hybrid mass spectrometer (Thermo Fisher Scientific, Courtaboeuf, France).
Article Title: LC-HRMS data as a result of untargeted metabolomic profiling of human cerebrospinal fluid
Article Snippet: Samples were analyzed using two chromatographic separations: i) BEH Amide, 1.7 μm, 100 mm × 2.1 mm I.D. column (Waters, Massachusetts, US) in positive ionization mode and, ii) SeQuant® ZIC-pHILIC, 5 μm, 100 mm
Article Title: Evaluation of Different Tandem MS Acquisition Modes to Support Metabolite Annotation in Human Plasma Using Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometry for Untargeted Metabolomics
Article Snippet: The gradient elution was as follows: 0% B for 2 min, increasing to 70% B during 18 min, holding for 3 min at 70% B and then returning back to 0% B in 1 min and re-equilibrating the column for 7 min. For the second one (
Article Title: Induction of ferroptosis by singlet oxygen generated from naphthalene endoperoxide.
Article Snippet: Singlet
Article Title: Metabolomic signatures for visceral adiposity and dysglycaemia in Asian Chinese and Caucasian European adults: the cross-sectional TOFI_Asia study
Article Snippet: Chromatographic separation was carried out at 25 °C on a SeQuant® ZIC®-pHILIC 5 µm, 2.1 mm × 100 mm column (
Article Title: Identifying strategies to target the metabolic flexibility of tumours.
Article Snippet: The chromatographic separation was performed on a
Liquid Chromatography with Mass Spectroscopy:
Article Title: A sulfoglycolytic Entner-Doudoroff pathway in Rhizobium leguminosarum bv. trifolii SRDI565
Article Snippet: Metabolite extracts were transferred into 2 mL auto sampler vials with glass inserts and placed in the auto sampler kept at 4 °C prior to analysis. .. Metabolite separation was performed by injecting 7 µL of the extract into a