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Merck KGaA sequant zic philic column
In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
Sequant Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequant zic philic column/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sequant zic philic column - by Bioz Stars, 2021-07
86/100 stars

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1) Product Images from "Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum"

Article Title: Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2017.01601

In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
Figure Legend Snippet: In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

Techniques Used: In Vitro, Activity Assay, High Performance Liquid Chromatography, Concentration Assay, Mass Spectrometry

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Article Snippet: Samples were analyzed using two chromatographic separations: i) BEH Amide, 1.7 μm, 100 mm × 2.1 mm I.D. column (Waters, Massachusetts, US) in positive ionization mode and, ii) SeQuant® ZIC-pHILIC, 5 μm, 100 mm × 2.1 mm I.D. column (Merck, Darmstadt, Germany) with a SeQuant® ZIC-pHILIC, 5 μm, 20 mm × 2.1 mm I.D. guard column (Merck, Darmstadt, Germany) in negative ionization mode.

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Article Snippet: The gradient elution was as follows: 0% B for 2 min, increasing to 70% B during 18 min, holding for 3 min at 70% B and then returning back to 0% B in 1 min and re-equilibrating the column for 7 min. For the second one (zHILIC), separations were performed on a SeQuant® Zic-pHILIC column (150 × 2.1 mm, 5 µm) and the appropriate guard kit (Merck, Darmstadt, Germany).

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Article Snippet: Chromatographic separation was carried out at 25 °C on a SeQuant® ZIC®-pHILIC 5 µm, 2.1 mm × 100 mm column (Merck, Darmstadt, Germany) with the following solvent system: A = 10 mM ammonium formate in water, B = 0.1% formic acid in acetonitrile.

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Article Snippet: Metabolite extracts were transferred into 2 mL auto sampler vials with glass inserts and placed in the auto sampler kept at 4 °C prior to analysis. .. Metabolite separation was performed by injecting 7 µL of the extract into a SeQuant® ZIC-pHILIC PEEK coated column (150 mm × 4.6 mm, 5 µm polymer, Merck Millipore) maintained at 25°C, with a gradient of solvent A (20 mM ammonium carbonate, pH 9.0, Sigma-Aldrich) and solvent B (100% acetonitrile, Hypergrade for LCMS LiChrosolv, Merck) at a flow rate of 0.3 mL/min. ..

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    Merck KGaA sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic column/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Guard Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic guard column/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic guard column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Journal: Frontiers in Microbiology

    Article Title: Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum

    doi: 10.3389/fmicb.2017.01601

    Figure Lengend Snippet: In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Article Snippet: HPLC analysis of all assay reactions was performed using a LaChrome ULTRA system (HITACHI) and a SeQuant ZIC-pHILIC column (Merck Millipore).

    Techniques: In Vitro, Activity Assay, High Performance Liquid Chromatography, Concentration Assay, Mass Spectrometry