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Merck KGaA semithin sections
A , light microscopy of <t>semithin</t> sections of Kir4.1 +/+ and Kir4.1 −/− stomach mucosae. In both genotypes, droplets of varying size can be observed in the cells directly lining the lumen, indicative of milk absorption. Some of the PCs in
Semithin Sections, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 6 article reviews
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92/100 stars

Images

1) Product Images from "Kir4.1 Channel Expression Is Essential for Parietal Cell Control of Acid Secretion *"

Article Title: Kir4.1 Channel Expression Is Essential for Parietal Cell Control of Acid Secretion *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.151191

A , light microscopy of semithin sections of Kir4.1 +/+ and Kir4.1 −/− stomach mucosae. In both genotypes, droplets of varying size can be observed in the cells directly lining the lumen, indicative of milk absorption. Some of the PCs in
Figure Legend Snippet: A , light microscopy of semithin sections of Kir4.1 +/+ and Kir4.1 −/− stomach mucosae. In both genotypes, droplets of varying size can be observed in the cells directly lining the lumen, indicative of milk absorption. Some of the PCs in

Techniques Used: Light Microscopy

2) Product Images from "Pathological alterations in liver injury following congestive heart failure induced by volume overload in rats"

Article Title: Pathological alterations in liver injury following congestive heart failure induced by volume overload in rats

Journal: PLoS ONE

doi: 10.1371/journal.pone.0184161

Light microscopic photographs of representative haematoxylin-eosin and toluidine blue stained liver sections. A ) Note normal lobular structure with hepatocytes having prominent rounded nuclei. B-D ) Liver sections of ACF rats show pyknotic nuclei (PN), cytoplasmic vacuolization, sinusoidal dilation (SD), leukocyte infiltration (Leu) massive breakdown of hepatocytes (*), and congestive blood vessels (double arrows) as well as a crescent-shaped condensation of nuclear chromatin (arrow). E ) Semithin liver sections of control rats were stained in 1% toluidine blue showing normal branching and anastomosing hepatocyte cords separated by hepatic blood sinusoids. Note, hepatocytes contain prominent rounded nuclei (N). F ) Semithin liver sections of ACF rats show hepatocytes with large vacuoles (arrow head) in the periportal area, heterogeneous parenchyma, which consists of dark and compact hepatocytes flanked by ballooned cells (also called apoptotic cells) (arrow) with nuclear degeneration (ND). Bars = 20 μm.
Figure Legend Snippet: Light microscopic photographs of representative haematoxylin-eosin and toluidine blue stained liver sections. A ) Note normal lobular structure with hepatocytes having prominent rounded nuclei. B-D ) Liver sections of ACF rats show pyknotic nuclei (PN), cytoplasmic vacuolization, sinusoidal dilation (SD), leukocyte infiltration (Leu) massive breakdown of hepatocytes (*), and congestive blood vessels (double arrows) as well as a crescent-shaped condensation of nuclear chromatin (arrow). E ) Semithin liver sections of control rats were stained in 1% toluidine blue showing normal branching and anastomosing hepatocyte cords separated by hepatic blood sinusoids. Note, hepatocytes contain prominent rounded nuclei (N). F ) Semithin liver sections of ACF rats show hepatocytes with large vacuoles (arrow head) in the periportal area, heterogeneous parenchyma, which consists of dark and compact hepatocytes flanked by ballooned cells (also called apoptotic cells) (arrow) with nuclear degeneration (ND). Bars = 20 μm.

Techniques Used: Staining

3) Product Images from "Deafferentation-Induced Redistribution of MMP-2, but Not of MMP-9, Depends on the Emergence of GAP-43 Positive Axons in the Adult Rat Cochlear Nucleus"

Article Title: Deafferentation-Induced Redistribution of MMP-2, but Not of MMP-9, Depends on the Emergence of GAP-43 Positive Axons in the Adult Rat Cochlear Nucleus

Journal: Neural Plasticity

doi: 10.1155/2011/859359

Semithin sections through AVCN stained for MMP-9 and MMP-2 immunoreactivity following cochlear ablation, and their quantitative evaluation. (a) By POD7, MMP-2 immunoreactivity showed up as particles. (b) Counterstaining of the same section with methylene blue, visualizing cellular boundaries. Lines indicate the regions of interest (ROI) sparing the cell nucleus (nu). (c) Same section as in (a) with the superimposed ROIs. Particles detected by computer-aided image analysis are indicated in red. Scale bar for (a)–(c) 20 μ m. (d) Subcellular redistribution of MMP-9 immunoreactive particles in AVCN neurons in control animals (ctrl) and at POD1, 3 and 7 following cochlear ablation. Bars indicate particle density in ROI 1 and ROI 2 . ns designates insignificant changes within ROI 2 at any time. (e), (f) Changes in MMP-9 particle density in cytoplasm (e) and neuropil (f) of AVCN neurons in controls (ctrl) and by POD1, 3 and 7 following cochlear ablation. Bars indicate left-to-right (l/r) or ipsilateral-to-contralateral (i/c) ratio of particle density. (g) Subcellular redistribution of MMP-2 immunoreactive particles within the cytoplasm of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7. (h), (i) Changes in MMP-2 particle density in cytoplasm (h) and neuropil (i) of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7 following cochlear ablation.
Figure Legend Snippet: Semithin sections through AVCN stained for MMP-9 and MMP-2 immunoreactivity following cochlear ablation, and their quantitative evaluation. (a) By POD7, MMP-2 immunoreactivity showed up as particles. (b) Counterstaining of the same section with methylene blue, visualizing cellular boundaries. Lines indicate the regions of interest (ROI) sparing the cell nucleus (nu). (c) Same section as in (a) with the superimposed ROIs. Particles detected by computer-aided image analysis are indicated in red. Scale bar for (a)–(c) 20 μ m. (d) Subcellular redistribution of MMP-9 immunoreactive particles in AVCN neurons in control animals (ctrl) and at POD1, 3 and 7 following cochlear ablation. Bars indicate particle density in ROI 1 and ROI 2 . ns designates insignificant changes within ROI 2 at any time. (e), (f) Changes in MMP-9 particle density in cytoplasm (e) and neuropil (f) of AVCN neurons in controls (ctrl) and by POD1, 3 and 7 following cochlear ablation. Bars indicate left-to-right (l/r) or ipsilateral-to-contralateral (i/c) ratio of particle density. (g) Subcellular redistribution of MMP-2 immunoreactive particles within the cytoplasm of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7. (h), (i) Changes in MMP-2 particle density in cytoplasm (h) and neuropil (i) of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7 following cochlear ablation.

Techniques Used: Staining

Related Articles

Light Microscopy:

Article Title: The Secreted Aspartyl Proteinases Sap1 and Sap2 Cause Tissue Damage in an In Vitro Model of Vaginal Candidiasis Based on Reconstituted Human Vaginal Epithelium
Article Snippet: .. Semithin sections (1 μm) were studied with a light microscope after staining with 1% toluidine blue and 1% pyronine G (Merck, Darmstadt, Germany). .. Postembedding immunogold labeling was carried out for intracellular detection of Sap antigen in SC5314-infected samples.

Article Title: Pathological alterations in liver injury following congestive heart failure induced by volume overload in rats
Article Snippet: .. Semithin sections of samples were stained 1 to 2 minutes in 1% Toluidine Blue (Merck, Darmstadt, Germany), rinsed several times in purified water, and examined under a light microscope (Axiophot 100; Zeiss, Jena, Germany). .. Apoptosis assay The staining method for liver apoptosis was performed in situ using Chemicon Apo-Direct Tunel Assay Kit (Merck Millipore, Darmstadt, Germany) for the detection of the internucleosomal DNA fragmentation, characteristic of apoptosis according to the manufacturer’s instructions.

Article Title: Histopathological Changes in the Kidney following Congestive Heart Failure by Volume Overload in Rats
Article Snippet: .. Semithin sections were stained 1 to 2 minutes in 1% Toluidine Blue (Merck, Darmstadt, Germany), rinsed several times in purified water, and examined under a light microscope (Axiophot 100; Zeiss, Germany). .. Apoptosis Assay The assessment of liver apoptosis was performed in situ using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assays (Chemicon Apo-Direct Tunel Assay Kit; Merck Millipore, Darmstadt, Germany) for the detection of the internucleosomal DNA fragmentation, characteristic for apoptosis according to the manufacturer's instructions.

Article Title: Effects of the Human Immunodeficiency Virus (HIV) Proteinase Inhibitors Saquinavir and Indinavir on In Vitro Activities of Secreted Aspartyl Proteinases of Candida albicans Isolates from HIV-Infected Patients
Article Snippet: .. RHE was incubated at 37°C with 5% CO2 at 100% humidity for 12 h. Semithin sections (1 μm) were studied with a light microscope after staining with 1% toluidine blue and 1% pyronine G (Merck, Darmstadt, Germany). .. Inhibition of Sap activity by SQV, IDV, and pepstatin A was observed for all three substrates in a dose-dependent manner (data not shown).

Staining:

Article Title: Multiple Phenotypes in Adult Mice following Inactivation of the Coxsackievirus and Adenovirus Receptor (Car) Gene
Article Snippet: .. Semithin sections were cut and stained with Toluidinblue O (Merck, Darmstadt, Germany) and used for light microscopic analysis. .. Ultrathin section (approximately 40–50 nm) were cut and contrasted with uranyl acetate (Merck, Darmstadt, Germany) followed by lead citrate (Merck, Darmstadt, Germany) and examined in a Leo 906 transmission electron microscope at 80 kV (Park et al , 2007).

Article Title: The Secreted Aspartyl Proteinases Sap1 and Sap2 Cause Tissue Damage in an In Vitro Model of Vaginal Candidiasis Based on Reconstituted Human Vaginal Epithelium
Article Snippet: .. Semithin sections (1 μm) were studied with a light microscope after staining with 1% toluidine blue and 1% pyronine G (Merck, Darmstadt, Germany). .. Postembedding immunogold labeling was carried out for intracellular detection of Sap antigen in SC5314-infected samples.

Article Title: Pathological alterations in liver injury following congestive heart failure induced by volume overload in rats
Article Snippet: .. Semithin sections of samples were stained 1 to 2 minutes in 1% Toluidine Blue (Merck, Darmstadt, Germany), rinsed several times in purified water, and examined under a light microscope (Axiophot 100; Zeiss, Jena, Germany). .. Apoptosis assay The staining method for liver apoptosis was performed in situ using Chemicon Apo-Direct Tunel Assay Kit (Merck Millipore, Darmstadt, Germany) for the detection of the internucleosomal DNA fragmentation, characteristic of apoptosis according to the manufacturer’s instructions.

Article Title: Histopathological Changes in the Kidney following Congestive Heart Failure by Volume Overload in Rats
Article Snippet: .. Semithin sections were stained 1 to 2 minutes in 1% Toluidine Blue (Merck, Darmstadt, Germany), rinsed several times in purified water, and examined under a light microscope (Axiophot 100; Zeiss, Germany). .. Apoptosis Assay The assessment of liver apoptosis was performed in situ using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assays (Chemicon Apo-Direct Tunel Assay Kit; Merck Millipore, Darmstadt, Germany) for the detection of the internucleosomal DNA fragmentation, characteristic for apoptosis according to the manufacturer's instructions.

Article Title: Effects of the Human Immunodeficiency Virus (HIV) Proteinase Inhibitors Saquinavir and Indinavir on In Vitro Activities of Secreted Aspartyl Proteinases of Candida albicans Isolates from HIV-Infected Patients
Article Snippet: .. RHE was incubated at 37°C with 5% CO2 at 100% humidity for 12 h. Semithin sections (1 μm) were studied with a light microscope after staining with 1% toluidine blue and 1% pyronine G (Merck, Darmstadt, Germany). .. Inhibition of Sap activity by SQV, IDV, and pepstatin A was observed for all three substrates in a dose-dependent manner (data not shown).

Incubation:

Article Title: Effects of the Human Immunodeficiency Virus (HIV) Proteinase Inhibitors Saquinavir and Indinavir on In Vitro Activities of Secreted Aspartyl Proteinases of Candida albicans Isolates from HIV-Infected Patients
Article Snippet: .. RHE was incubated at 37°C with 5% CO2 at 100% humidity for 12 h. Semithin sections (1 μm) were studied with a light microscope after staining with 1% toluidine blue and 1% pyronine G (Merck, Darmstadt, Germany). .. Inhibition of Sap activity by SQV, IDV, and pepstatin A was observed for all three substrates in a dose-dependent manner (data not shown).

Purification:

Article Title: Pathological alterations in liver injury following congestive heart failure induced by volume overload in rats
Article Snippet: .. Semithin sections of samples were stained 1 to 2 minutes in 1% Toluidine Blue (Merck, Darmstadt, Germany), rinsed several times in purified water, and examined under a light microscope (Axiophot 100; Zeiss, Jena, Germany). .. Apoptosis assay The staining method for liver apoptosis was performed in situ using Chemicon Apo-Direct Tunel Assay Kit (Merck Millipore, Darmstadt, Germany) for the detection of the internucleosomal DNA fragmentation, characteristic of apoptosis according to the manufacturer’s instructions.

Article Title: Histopathological Changes in the Kidney following Congestive Heart Failure by Volume Overload in Rats
Article Snippet: .. Semithin sections were stained 1 to 2 minutes in 1% Toluidine Blue (Merck, Darmstadt, Germany), rinsed several times in purified water, and examined under a light microscope (Axiophot 100; Zeiss, Germany). .. Apoptosis Assay The assessment of liver apoptosis was performed in situ using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assays (Chemicon Apo-Direct Tunel Assay Kit; Merck Millipore, Darmstadt, Germany) for the detection of the internucleosomal DNA fragmentation, characteristic for apoptosis according to the manufacturer's instructions.

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    Merck KGaA semithin sections
    A , light microscopy of <t>semithin</t> sections of Kir4.1 +/+ and Kir4.1 −/− stomach mucosae. In both genotypes, droplets of varying size can be observed in the cells directly lining the lumen, indicative of milk absorption. Some of the PCs in
    Semithin Sections, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semithin sections/product/Merck KGaA
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    semithin sections - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    A , light microscopy of semithin sections of Kir4.1 +/+ and Kir4.1 −/− stomach mucosae. In both genotypes, droplets of varying size can be observed in the cells directly lining the lumen, indicative of milk absorption. Some of the PCs in

    Journal: The Journal of Biological Chemistry

    Article Title: Kir4.1 Channel Expression Is Essential for Parietal Cell Control of Acid Secretion *

    doi: 10.1074/jbc.M110.151191

    Figure Lengend Snippet: A , light microscopy of semithin sections of Kir4.1 +/+ and Kir4.1 −/− stomach mucosae. In both genotypes, droplets of varying size can be observed in the cells directly lining the lumen, indicative of milk absorption. Some of the PCs in

    Article Snippet: Semithin sections (1 μm) of these samples were collected on glass slides pretreated with 3-(triethoxysilyl)propylamide (silane; Merck, Darmstadt, Germany).

    Techniques: Light Microscopy

    Light microscopic photographs of representative haematoxylin-eosin and toluidine blue stained liver sections. A ) Note normal lobular structure with hepatocytes having prominent rounded nuclei. B-D ) Liver sections of ACF rats show pyknotic nuclei (PN), cytoplasmic vacuolization, sinusoidal dilation (SD), leukocyte infiltration (Leu) massive breakdown of hepatocytes (*), and congestive blood vessels (double arrows) as well as a crescent-shaped condensation of nuclear chromatin (arrow). E ) Semithin liver sections of control rats were stained in 1% toluidine blue showing normal branching and anastomosing hepatocyte cords separated by hepatic blood sinusoids. Note, hepatocytes contain prominent rounded nuclei (N). F ) Semithin liver sections of ACF rats show hepatocytes with large vacuoles (arrow head) in the periportal area, heterogeneous parenchyma, which consists of dark and compact hepatocytes flanked by ballooned cells (also called apoptotic cells) (arrow) with nuclear degeneration (ND). Bars = 20 μm.

    Journal: PLoS ONE

    Article Title: Pathological alterations in liver injury following congestive heart failure induced by volume overload in rats

    doi: 10.1371/journal.pone.0184161

    Figure Lengend Snippet: Light microscopic photographs of representative haematoxylin-eosin and toluidine blue stained liver sections. A ) Note normal lobular structure with hepatocytes having prominent rounded nuclei. B-D ) Liver sections of ACF rats show pyknotic nuclei (PN), cytoplasmic vacuolization, sinusoidal dilation (SD), leukocyte infiltration (Leu) massive breakdown of hepatocytes (*), and congestive blood vessels (double arrows) as well as a crescent-shaped condensation of nuclear chromatin (arrow). E ) Semithin liver sections of control rats were stained in 1% toluidine blue showing normal branching and anastomosing hepatocyte cords separated by hepatic blood sinusoids. Note, hepatocytes contain prominent rounded nuclei (N). F ) Semithin liver sections of ACF rats show hepatocytes with large vacuoles (arrow head) in the periportal area, heterogeneous parenchyma, which consists of dark and compact hepatocytes flanked by ballooned cells (also called apoptotic cells) (arrow) with nuclear degeneration (ND). Bars = 20 μm.

    Article Snippet: Semithin sections of samples were stained 1 to 2 minutes in 1% Toluidine Blue (Merck, Darmstadt, Germany), rinsed several times in purified water, and examined under a light microscope (Axiophot 100; Zeiss, Jena, Germany).

    Techniques: Staining

    Semithin sections through AVCN stained for MMP-9 and MMP-2 immunoreactivity following cochlear ablation, and their quantitative evaluation. (a) By POD7, MMP-2 immunoreactivity showed up as particles. (b) Counterstaining of the same section with methylene blue, visualizing cellular boundaries. Lines indicate the regions of interest (ROI) sparing the cell nucleus (nu). (c) Same section as in (a) with the superimposed ROIs. Particles detected by computer-aided image analysis are indicated in red. Scale bar for (a)–(c) 20 μ m. (d) Subcellular redistribution of MMP-9 immunoreactive particles in AVCN neurons in control animals (ctrl) and at POD1, 3 and 7 following cochlear ablation. Bars indicate particle density in ROI 1 and ROI 2 . ns designates insignificant changes within ROI 2 at any time. (e), (f) Changes in MMP-9 particle density in cytoplasm (e) and neuropil (f) of AVCN neurons in controls (ctrl) and by POD1, 3 and 7 following cochlear ablation. Bars indicate left-to-right (l/r) or ipsilateral-to-contralateral (i/c) ratio of particle density. (g) Subcellular redistribution of MMP-2 immunoreactive particles within the cytoplasm of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7. (h), (i) Changes in MMP-2 particle density in cytoplasm (h) and neuropil (i) of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7 following cochlear ablation.

    Journal: Neural Plasticity

    Article Title: Deafferentation-Induced Redistribution of MMP-2, but Not of MMP-9, Depends on the Emergence of GAP-43 Positive Axons in the Adult Rat Cochlear Nucleus

    doi: 10.1155/2011/859359

    Figure Lengend Snippet: Semithin sections through AVCN stained for MMP-9 and MMP-2 immunoreactivity following cochlear ablation, and their quantitative evaluation. (a) By POD7, MMP-2 immunoreactivity showed up as particles. (b) Counterstaining of the same section with methylene blue, visualizing cellular boundaries. Lines indicate the regions of interest (ROI) sparing the cell nucleus (nu). (c) Same section as in (a) with the superimposed ROIs. Particles detected by computer-aided image analysis are indicated in red. Scale bar for (a)–(c) 20 μ m. (d) Subcellular redistribution of MMP-9 immunoreactive particles in AVCN neurons in control animals (ctrl) and at POD1, 3 and 7 following cochlear ablation. Bars indicate particle density in ROI 1 and ROI 2 . ns designates insignificant changes within ROI 2 at any time. (e), (f) Changes in MMP-9 particle density in cytoplasm (e) and neuropil (f) of AVCN neurons in controls (ctrl) and by POD1, 3 and 7 following cochlear ablation. Bars indicate left-to-right (l/r) or ipsilateral-to-contralateral (i/c) ratio of particle density. (g) Subcellular redistribution of MMP-2 immunoreactive particles within the cytoplasm of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7. (h), (i) Changes in MMP-2 particle density in cytoplasm (h) and neuropil (i) of AVCN neurons in control animals (ctrl) and by POD1, 3 and 7 following cochlear ablation.

    Article Snippet: After photographic documentation of the first 1 to 3 semithin sections cut , cover-slips were removed and the sections counterstained with methylene blue-azure (1% azure II in distilled water and 1% methylene blue (Merck, Darmstadt, Germany, 9211 and 6045, resp.) in borax, mixed to equal parts) to visualize boundaries of cell bodies and nuclei ( ).

    Techniques: Staining