Structured Review

Hamamatsu semithin sections
Immuno-EM of Cx29. These are images of teased fibers that were fixed in 0.5% glutaraldehyde, immunostained for Cx29 using DAB as the substrate for peroxidase, and processed for EM. Two teased fibers are illustrated in A , unstained <t>semithin</t> sections are shown in B and C , and thin sections photographed with an electron microscope are shown in D and E . In all panels , there is DAB throughout incisures ( arrowheads ) and in the innermost aspects of the juxtaparanodal/paranodal regions ( arrows ) of myelinating Schwann cells. SC , Schwann cell nucleus; M , myelin sheath; A , axon. Double arrowheads , Node of Ranvier. Scale bars: A–C , 10 μm; D, E , 1 μm.
Semithin Sections, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/semithin sections/product/Hamamatsu
Average 92 stars, based on 1 article reviews
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semithin sections - by Bioz Stars, 2020-08
92/100 stars

Images

1) Product Images from "Connexin29 Is Uniquely Distributed within Myelinating Glial Cells of the Central and Peripheral Nervous Systems"

Article Title: Connexin29 Is Uniquely Distributed within Myelinating Glial Cells of the Central and Peripheral Nervous Systems

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.22-15-06458.2002

Immuno-EM of Cx29. These are images of teased fibers that were fixed in 0.5% glutaraldehyde, immunostained for Cx29 using DAB as the substrate for peroxidase, and processed for EM. Two teased fibers are illustrated in A , unstained semithin sections are shown in B and C , and thin sections photographed with an electron microscope are shown in D and E . In all panels , there is DAB throughout incisures ( arrowheads ) and in the innermost aspects of the juxtaparanodal/paranodal regions ( arrows ) of myelinating Schwann cells. SC , Schwann cell nucleus; M , myelin sheath; A , axon. Double arrowheads , Node of Ranvier. Scale bars: A–C , 10 μm; D, E , 1 μm.
Figure Legend Snippet: Immuno-EM of Cx29. These are images of teased fibers that were fixed in 0.5% glutaraldehyde, immunostained for Cx29 using DAB as the substrate for peroxidase, and processed for EM. Two teased fibers are illustrated in A , unstained semithin sections are shown in B and C , and thin sections photographed with an electron microscope are shown in D and E . In all panels , there is DAB throughout incisures ( arrowheads ) and in the innermost aspects of the juxtaparanodal/paranodal regions ( arrows ) of myelinating Schwann cells. SC , Schwann cell nucleus; M , myelin sheath; A , axon. Double arrowheads , Node of Ranvier. Scale bars: A–C , 10 μm; D, E , 1 μm.

Techniques Used: Microscopy

2) Product Images from "Prenylation-Defective Human Connexin32 Mutants Are Normally Localized and Function Equivalently to Wild-Type Connexin32 in Myelinating Schwann Cells"

Article Title: Prenylation-Defective Human Connexin32 Mutants Are Normally Localized and Function Equivalently to Wild-Type Connexin32 in Myelinating Schwann Cells

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.1319-05.2005

Transgenic expression of C280G mutant protein rescues demyelination in Gjb1 -null mice. These are images of semithin sections of the femoral motor branch from TG + or TG - Gjb1 -/Y littermates from line 7M33 (at P158). The myelinated axons appear normal in the TG + nerves, whereas the TG - nerve has remyelinated (r) axons and macrophages containing myelin debris (m). The areas outlined by the rectangles in A and B are enlarged in C and D ( C and D are rotated in opposite directions). Scale bars, 10 μm.
Figure Legend Snippet: Transgenic expression of C280G mutant protein rescues demyelination in Gjb1 -null mice. These are images of semithin sections of the femoral motor branch from TG + or TG - Gjb1 -/Y littermates from line 7M33 (at P158). The myelinated axons appear normal in the TG + nerves, whereas the TG - nerve has remyelinated (r) axons and macrophages containing myelin debris (m). The areas outlined by the rectangles in A and B are enlarged in C and D ( C and D are rotated in opposite directions). Scale bars, 10 μm.

Techniques Used: Transgenic Assay, Expressing, Mutagenesis, Mouse Assay

3) Product Images from "Characterization of experimental diabetic neuropathy using multicontrast magnetic resonance neurography at ultra high field strength"

Article Title: Characterization of experimental diabetic neuropathy using multicontrast magnetic resonance neurography at ultra high field strength

Journal: Scientific Reports

doi: 10.1038/s41598-020-64585-1

Light microscopic analysis of the sciatic nerves from STZ-diabetic mice. ( a ) Gross anatomical findings of Toluidine blue stained semithin sciatic nerve sections from control and STZ animals using light microscopy (LM). Inset shows a representative region in x2-magnification. ( b – d ) Quantitative findings of the control (Ctrl) and the STZ groups (n = 3 each) comparing the three parameters b) ‘axon density’, ( c ) ‘myelin density’ (i.e. the myelin fraction within the nerve cross-sectional area (CSA)) and ( d ) ‘average myelination’ (i.e. myelin area per axon); n.s. = nonsignificant, unpaired two-sided t-test. ( e ) Histogram plot showing the frequency distribution of the equivalent axon radius within the population of all myelinated axons inside the nerve CSA. Each bar indicates group mean ± s.d. (n = 3 each). *p
Figure Legend Snippet: Light microscopic analysis of the sciatic nerves from STZ-diabetic mice. ( a ) Gross anatomical findings of Toluidine blue stained semithin sciatic nerve sections from control and STZ animals using light microscopy (LM). Inset shows a representative region in x2-magnification. ( b – d ) Quantitative findings of the control (Ctrl) and the STZ groups (n = 3 each) comparing the three parameters b) ‘axon density’, ( c ) ‘myelin density’ (i.e. the myelin fraction within the nerve cross-sectional area (CSA)) and ( d ) ‘average myelination’ (i.e. myelin area per axon); n.s. = nonsignificant, unpaired two-sided t-test. ( e ) Histogram plot showing the frequency distribution of the equivalent axon radius within the population of all myelinated axons inside the nerve CSA. Each bar indicates group mean ± s.d. (n = 3 each). *p

Techniques Used: Mouse Assay, Staining, Light Microscopy

Related Articles

Light Microscopy:

Article Title: Prenylation-Defective Human Connexin32 Mutants Are Normally Localized and Function Equivalently to Wild-Type Connexin32 in Myelinating Schwann Cells
Article Snippet: .. Semithin sections of the femoral motor and sensory branches and sciatic nerve were stained with toluidine blue, examined by light microscopy, and imaged with Leica DMR light microscope equipped with a cooled Hamamatsu camera, followed by image manipulation with Adobe Photoshop. .. For a quantitative analysis of demyelinated and remyelinated axons, digital images (OpenLab software; Improvision, Lexington, MA) were made of single transverse semithin sections femoral motor nerve.

Staining:

Article Title: Characterization of experimental diabetic neuropathy using multicontrast magnetic resonance neurography at ultra high field strength
Article Snippet: .. Semithin sections of 0.9 µm were stained with toluidine blue for light microscopical examination (Hamamatsu NanoZoomer Digital Pathology, Hamamatsu Photonics, K.K., Japan) and digitized using NIS-Elements BR 3.00 Imaging Software (Nikon, Chiyoda, Japan). .. Selected areas of blocks were cut into 60-80 nm ultrathin sections, treated with uranyl acetate and lead citrate, and examined with an electron microscope JEM 1400 equipped with a 2 K TVIPS CCD Camera TemCam F216.

Article Title: Prenylation-Defective Human Connexin32 Mutants Are Normally Localized and Function Equivalently to Wild-Type Connexin32 in Myelinating Schwann Cells
Article Snippet: .. Semithin sections of the femoral motor and sensory branches and sciatic nerve were stained with toluidine blue, examined by light microscopy, and imaged with Leica DMR light microscope equipped with a cooled Hamamatsu camera, followed by image manipulation with Adobe Photoshop. .. For a quantitative analysis of demyelinated and remyelinated axons, digital images (OpenLab software; Improvision, Lexington, MA) were made of single transverse semithin sections femoral motor nerve.

Generated:

Article Title: Connexin29 Is Uniquely Distributed within Myelinating Glial Cells of the Central and Peripheral Nervous Systems
Article Snippet: .. Light microscopic images of the teased fibers and semithin sections were generated with a cooled Hamamatsu camera. .. Electron micrographs were printed and scanned; these images were imported into Adobe PhotoShop and assembled.

Imaging:

Article Title: Characterization of experimental diabetic neuropathy using multicontrast magnetic resonance neurography at ultra high field strength
Article Snippet: .. Semithin sections of 0.9 µm were stained with toluidine blue for light microscopical examination (Hamamatsu NanoZoomer Digital Pathology, Hamamatsu Photonics, K.K., Japan) and digitized using NIS-Elements BR 3.00 Imaging Software (Nikon, Chiyoda, Japan). .. Selected areas of blocks were cut into 60-80 nm ultrathin sections, treated with uranyl acetate and lead citrate, and examined with an electron microscope JEM 1400 equipped with a 2 K TVIPS CCD Camera TemCam F216.

Software:

Article Title: Characterization of experimental diabetic neuropathy using multicontrast magnetic resonance neurography at ultra high field strength
Article Snippet: .. Semithin sections of 0.9 µm were stained with toluidine blue for light microscopical examination (Hamamatsu NanoZoomer Digital Pathology, Hamamatsu Photonics, K.K., Japan) and digitized using NIS-Elements BR 3.00 Imaging Software (Nikon, Chiyoda, Japan). .. Selected areas of blocks were cut into 60-80 nm ultrathin sections, treated with uranyl acetate and lead citrate, and examined with an electron microscope JEM 1400 equipped with a 2 K TVIPS CCD Camera TemCam F216.

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    Hamamatsu semithin sections
    Immuno-EM of Cx29. These are images of teased fibers that were fixed in 0.5% glutaraldehyde, immunostained for Cx29 using DAB as the substrate for peroxidase, and processed for EM. Two teased fibers are illustrated in A , unstained <t>semithin</t> sections are shown in B and C , and thin sections photographed with an electron microscope are shown in D and E . In all panels , there is DAB throughout incisures ( arrowheads ) and in the innermost aspects of the juxtaparanodal/paranodal regions ( arrows ) of myelinating Schwann cells. SC , Schwann cell nucleus; M , myelin sheath; A , axon. Double arrowheads , Node of Ranvier. Scale bars: A–C , 10 μm; D, E , 1 μm.
    Semithin Sections, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semithin sections/product/Hamamatsu
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    semithin sections - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Immuno-EM of Cx29. These are images of teased fibers that were fixed in 0.5% glutaraldehyde, immunostained for Cx29 using DAB as the substrate for peroxidase, and processed for EM. Two teased fibers are illustrated in A , unstained semithin sections are shown in B and C , and thin sections photographed with an electron microscope are shown in D and E . In all panels , there is DAB throughout incisures ( arrowheads ) and in the innermost aspects of the juxtaparanodal/paranodal regions ( arrows ) of myelinating Schwann cells. SC , Schwann cell nucleus; M , myelin sheath; A , axon. Double arrowheads , Node of Ranvier. Scale bars: A–C , 10 μm; D, E , 1 μm.

    Journal: The Journal of Neuroscience

    Article Title: Connexin29 Is Uniquely Distributed within Myelinating Glial Cells of the Central and Peripheral Nervous Systems

    doi: 10.1523/JNEUROSCI.22-15-06458.2002

    Figure Lengend Snippet: Immuno-EM of Cx29. These are images of teased fibers that were fixed in 0.5% glutaraldehyde, immunostained for Cx29 using DAB as the substrate for peroxidase, and processed for EM. Two teased fibers are illustrated in A , unstained semithin sections are shown in B and C , and thin sections photographed with an electron microscope are shown in D and E . In all panels , there is DAB throughout incisures ( arrowheads ) and in the innermost aspects of the juxtaparanodal/paranodal regions ( arrows ) of myelinating Schwann cells. SC , Schwann cell nucleus; M , myelin sheath; A , axon. Double arrowheads , Node of Ranvier. Scale bars: A–C , 10 μm; D, E , 1 μm.

    Article Snippet: Light microscopic images of the teased fibers and semithin sections were generated with a cooled Hamamatsu camera.

    Techniques: Microscopy

    Transgenic expression of C280G mutant protein rescues demyelination in Gjb1 -null mice. These are images of semithin sections of the femoral motor branch from TG + or TG - Gjb1 -/Y littermates from line 7M33 (at P158). The myelinated axons appear normal in the TG + nerves, whereas the TG - nerve has remyelinated (r) axons and macrophages containing myelin debris (m). The areas outlined by the rectangles in A and B are enlarged in C and D ( C and D are rotated in opposite directions). Scale bars, 10 μm.

    Journal: The Journal of Neuroscience

    Article Title: Prenylation-Defective Human Connexin32 Mutants Are Normally Localized and Function Equivalently to Wild-Type Connexin32 in Myelinating Schwann Cells

    doi: 10.1523/JNEUROSCI.1319-05.2005

    Figure Lengend Snippet: Transgenic expression of C280G mutant protein rescues demyelination in Gjb1 -null mice. These are images of semithin sections of the femoral motor branch from TG + or TG - Gjb1 -/Y littermates from line 7M33 (at P158). The myelinated axons appear normal in the TG + nerves, whereas the TG - nerve has remyelinated (r) axons and macrophages containing myelin debris (m). The areas outlined by the rectangles in A and B are enlarged in C and D ( C and D are rotated in opposite directions). Scale bars, 10 μm.

    Article Snippet: Semithin sections of the femoral motor and sensory branches and sciatic nerve were stained with toluidine blue, examined by light microscopy, and imaged with Leica DMR light microscope equipped with a cooled Hamamatsu camera, followed by image manipulation with Adobe Photoshop.

    Techniques: Transgenic Assay, Expressing, Mutagenesis, Mouse Assay

    Light microscopic analysis of the sciatic nerves from STZ-diabetic mice. ( a ) Gross anatomical findings of Toluidine blue stained semithin sciatic nerve sections from control and STZ animals using light microscopy (LM). Inset shows a representative region in x2-magnification. ( b – d ) Quantitative findings of the control (Ctrl) and the STZ groups (n = 3 each) comparing the three parameters b) ‘axon density’, ( c ) ‘myelin density’ (i.e. the myelin fraction within the nerve cross-sectional area (CSA)) and ( d ) ‘average myelination’ (i.e. myelin area per axon); n.s. = nonsignificant, unpaired two-sided t-test. ( e ) Histogram plot showing the frequency distribution of the equivalent axon radius within the population of all myelinated axons inside the nerve CSA. Each bar indicates group mean ± s.d. (n = 3 each). *p

    Journal: Scientific Reports

    Article Title: Characterization of experimental diabetic neuropathy using multicontrast magnetic resonance neurography at ultra high field strength

    doi: 10.1038/s41598-020-64585-1

    Figure Lengend Snippet: Light microscopic analysis of the sciatic nerves from STZ-diabetic mice. ( a ) Gross anatomical findings of Toluidine blue stained semithin sciatic nerve sections from control and STZ animals using light microscopy (LM). Inset shows a representative region in x2-magnification. ( b – d ) Quantitative findings of the control (Ctrl) and the STZ groups (n = 3 each) comparing the three parameters b) ‘axon density’, ( c ) ‘myelin density’ (i.e. the myelin fraction within the nerve cross-sectional area (CSA)) and ( d ) ‘average myelination’ (i.e. myelin area per axon); n.s. = nonsignificant, unpaired two-sided t-test. ( e ) Histogram plot showing the frequency distribution of the equivalent axon radius within the population of all myelinated axons inside the nerve CSA. Each bar indicates group mean ± s.d. (n = 3 each). *p

    Article Snippet: Semithin sections of 0.9 µm were stained with toluidine blue for light microscopical examination (Hamamatsu NanoZoomer Digital Pathology, Hamamatsu Photonics, K.K., Japan) and digitized using NIS-Elements BR 3.00 Imaging Software (Nikon, Chiyoda, Japan).

    Techniques: Mouse Assay, Staining, Light Microscopy