semi preparative scale carbopac pa1 column  (Thermo Fisher)


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    Structured Review

    Thermo Fisher semi preparative scale carbopac pa1 column
    Semi Preparative Scale Carbopac Pa1 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative scale carbopac pa1 column/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    semi preparative scale carbopac pa1 column - by Bioz Stars, 2020-09
    85/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Determination of the primary structure and carboxyl pKAs of heparin-derived oligosaccharides by band-selective homonuclear-decoupled two-dimensional 1H NMR
    Article Snippet: .. The tetra-, hexa-, and octasaccharides studied in this work were isolated from the size-uniform tetra-, hexa-, and octasaccharide fractions by strong anion exchange HPLC with a Dionex 500 ion chromatography system equipped with a GP40 gradient pump, an AD20 UV/visible detector and a Dionex semi-preparative scale CarboPac PA1 column. .. The oligosaccharides were eluted with a linear gradient of 70 mM pH 3 phosphate buffer (solvent A) and 70 mM pH 3 phosphate buffer containing 2 M NaCl (solvent B) at a flow rate of 3.3 mL/min.

    Isolation:

    Article Title: Determination of the primary structure and carboxyl pKAs of heparin-derived oligosaccharides by band-selective homonuclear-decoupled two-dimensional 1H NMR
    Article Snippet: .. The tetra-, hexa-, and octasaccharides studied in this work were isolated from the size-uniform tetra-, hexa-, and octasaccharide fractions by strong anion exchange HPLC with a Dionex 500 ion chromatography system equipped with a GP40 gradient pump, an AD20 UV/visible detector and a Dionex semi-preparative scale CarboPac PA1 column. .. The oligosaccharides were eluted with a linear gradient of 70 mM pH 3 phosphate buffer (solvent A) and 70 mM pH 3 phosphate buffer containing 2 M NaCl (solvent B) at a flow rate of 3.3 mL/min.

    Ion Chromatography:

    Article Title: Determination of the primary structure and carboxyl pKAs of heparin-derived oligosaccharides by band-selective homonuclear-decoupled two-dimensional 1H NMR
    Article Snippet: .. The tetra-, hexa-, and octasaccharides studied in this work were isolated from the size-uniform tetra-, hexa-, and octasaccharide fractions by strong anion exchange HPLC with a Dionex 500 ion chromatography system equipped with a GP40 gradient pump, an AD20 UV/visible detector and a Dionex semi-preparative scale CarboPac PA1 column. .. The oligosaccharides were eluted with a linear gradient of 70 mM pH 3 phosphate buffer (solvent A) and 70 mM pH 3 phosphate buffer containing 2 M NaCl (solvent B) at a flow rate of 3.3 mL/min.

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    Thermo Fisher carbopac pa1 column
    Structural analysis of xyloside primed GAG chains. HUVEC cultures were treated with xylosides 3 or 4 at 1, 10 or 100 μM with [S 35 ]-SO 4 and incubated for 2 days. GAGs from the media and cell extracts were then purified over a DEAE column. BLUE: Endogenous GAGs, GREEN: Xyloside 3 primed GAGs, and RED: Xyloside 4 primed GAGs. (A) 100,000 CPM of purified radiolabeled GAGs when primed at 1 or 10 μM, and 200,000 CPM of GAGs when primed at 100 μM were analyzed separately over a DEAE-3SW column for anion-exchange chromatography. When primed at 10 and 100 μM, xyloside 3 primed GAGs have similar HS/CS ratios to the endogenous GAGs while xyloside 4 primed GAGs have approximately 3–4 times the amount of CS than HS. When primed at 1 μM, both 3 and 4 primed more CS than HS, although 4 still primes relatively more CS than 3 (B). Radiolabeled GAGs were digested with heparitinase I/II/III and analyzed over the <t>CarboPac</t> analytical <t>PA1</t> column. Generally, xyloside primed GAGs have similar HS compositions to endogenous GAGs with the exception of xyloside 4 primed at 100 μM that has an extra disulfated peak. (C) Radiolabeled GAGs were digested with chondroitinase ABC and analyzed over YMC PA-G column. The CS disaccharides primed by the xylosides have additional disulfated peaks compared to endogenous CS; All peaks were identified by matching with HS and CS disaccharide standards.
    Carbopac Pa1 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbopac pa1 column/product/Thermo Fisher
    Average 99 stars, based on 104 article reviews
    Price from $9.99 to $1999.99
    carbopac pa1 column - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    84
    Thermo Fisher carbopac pa1 cartridge column
    Structural analysis of xyloside primed GAG chains. HUVEC cultures were treated with xylosides 3 or 4 at 1, 10 or 100 μM with [S 35 ]-SO 4 and incubated for 2 days. GAGs from the media and cell extracts were then purified over a DEAE column. BLUE: Endogenous GAGs, GREEN: Xyloside 3 primed GAGs, and RED: Xyloside 4 primed GAGs. (A) 100,000 CPM of purified radiolabeled GAGs when primed at 1 or 10 μM, and 200,000 CPM of GAGs when primed at 100 μM were analyzed separately over a DEAE-3SW column for anion-exchange chromatography. When primed at 10 and 100 μM, xyloside 3 primed GAGs have similar HS/CS ratios to the endogenous GAGs while xyloside 4 primed GAGs have approximately 3–4 times the amount of CS than HS. When primed at 1 μM, both 3 and 4 primed more CS than HS, although 4 still primes relatively more CS than 3 (B). Radiolabeled GAGs were digested with heparitinase I/II/III and analyzed over the <t>CarboPac</t> analytical <t>PA1</t> column. Generally, xyloside primed GAGs have similar HS compositions to endogenous GAGs with the exception of xyloside 4 primed at 100 μM that has an extra disulfated peak. (C) Radiolabeled GAGs were digested with chondroitinase ABC and analyzed over YMC PA-G column. The CS disaccharides primed by the xylosides have additional disulfated peaks compared to endogenous CS; All peaks were identified by matching with HS and CS disaccharide standards.
    Carbopac Pa1 Cartridge Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbopac pa1 cartridge column/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    carbopac pa1 cartridge column - by Bioz Stars, 2020-09
    84/100 stars
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    Thermo Fisher 250 mm carbopac pa1 column
    Structural analysis of xyloside primed GAG chains. HUVEC cultures were treated with xylosides 3 or 4 at 1, 10 or 100 μM with [S 35 ]-SO 4 and incubated for 2 days. GAGs from the media and cell extracts were then purified over a DEAE column. BLUE: Endogenous GAGs, GREEN: Xyloside 3 primed GAGs, and RED: Xyloside 4 primed GAGs. (A) 100,000 CPM of purified radiolabeled GAGs when primed at 1 or 10 μM, and 200,000 CPM of GAGs when primed at 100 μM were analyzed separately over a DEAE-3SW column for anion-exchange chromatography. When primed at 10 and 100 μM, xyloside 3 primed GAGs have similar HS/CS ratios to the endogenous GAGs while xyloside 4 primed GAGs have approximately 3–4 times the amount of CS than HS. When primed at 1 μM, both 3 and 4 primed more CS than HS, although 4 still primes relatively more CS than 3 (B). Radiolabeled GAGs were digested with heparitinase I/II/III and analyzed over the <t>CarboPac</t> analytical <t>PA1</t> column. Generally, xyloside primed GAGs have similar HS compositions to endogenous GAGs with the exception of xyloside 4 primed at 100 μM that has an extra disulfated peak. (C) Radiolabeled GAGs were digested with chondroitinase ABC and analyzed over YMC PA-G column. The CS disaccharides primed by the xylosides have additional disulfated peaks compared to endogenous CS; All peaks were identified by matching with HS and CS disaccharide standards.
    250 Mm Carbopac Pa1 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/250 mm carbopac pa1 column/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    250 mm carbopac pa1 column - by Bioz Stars, 2020-09
    85/100 stars
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    Structural analysis of xyloside primed GAG chains. HUVEC cultures were treated with xylosides 3 or 4 at 1, 10 or 100 μM with [S 35 ]-SO 4 and incubated for 2 days. GAGs from the media and cell extracts were then purified over a DEAE column. BLUE: Endogenous GAGs, GREEN: Xyloside 3 primed GAGs, and RED: Xyloside 4 primed GAGs. (A) 100,000 CPM of purified radiolabeled GAGs when primed at 1 or 10 μM, and 200,000 CPM of GAGs when primed at 100 μM were analyzed separately over a DEAE-3SW column for anion-exchange chromatography. When primed at 10 and 100 μM, xyloside 3 primed GAGs have similar HS/CS ratios to the endogenous GAGs while xyloside 4 primed GAGs have approximately 3–4 times the amount of CS than HS. When primed at 1 μM, both 3 and 4 primed more CS than HS, although 4 still primes relatively more CS than 3 (B). Radiolabeled GAGs were digested with heparitinase I/II/III and analyzed over the CarboPac analytical PA1 column. Generally, xyloside primed GAGs have similar HS compositions to endogenous GAGs with the exception of xyloside 4 primed at 100 μM that has an extra disulfated peak. (C) Radiolabeled GAGs were digested with chondroitinase ABC and analyzed over YMC PA-G column. The CS disaccharides primed by the xylosides have additional disulfated peaks compared to endogenous CS; All peaks were identified by matching with HS and CS disaccharide standards.

    Journal: PLoS ONE

    Article Title: A glycan-based approach to therapeutic angiogenesis

    doi: 10.1371/journal.pone.0182301

    Figure Lengend Snippet: Structural analysis of xyloside primed GAG chains. HUVEC cultures were treated with xylosides 3 or 4 at 1, 10 or 100 μM with [S 35 ]-SO 4 and incubated for 2 days. GAGs from the media and cell extracts were then purified over a DEAE column. BLUE: Endogenous GAGs, GREEN: Xyloside 3 primed GAGs, and RED: Xyloside 4 primed GAGs. (A) 100,000 CPM of purified radiolabeled GAGs when primed at 1 or 10 μM, and 200,000 CPM of GAGs when primed at 100 μM were analyzed separately over a DEAE-3SW column for anion-exchange chromatography. When primed at 10 and 100 μM, xyloside 3 primed GAGs have similar HS/CS ratios to the endogenous GAGs while xyloside 4 primed GAGs have approximately 3–4 times the amount of CS than HS. When primed at 1 μM, both 3 and 4 primed more CS than HS, although 4 still primes relatively more CS than 3 (B). Radiolabeled GAGs were digested with heparitinase I/II/III and analyzed over the CarboPac analytical PA1 column. Generally, xyloside primed GAGs have similar HS compositions to endogenous GAGs with the exception of xyloside 4 primed at 100 μM that has an extra disulfated peak. (C) Radiolabeled GAGs were digested with chondroitinase ABC and analyzed over YMC PA-G column. The CS disaccharides primed by the xylosides have additional disulfated peaks compared to endogenous CS; All peaks were identified by matching with HS and CS disaccharide standards.

    Article Snippet: The HPLC columns used were DEAE-3SW column (7.5 mm × 7.5 cm, 10-μm particle size, Tosoh Bioscience LLC), Dionex CarboPac PA1 column (4 × 250 mm, 10-μm particle size, ThermoFisher) and YMC-Pack PA-G column (250 × 4.6 mm, 5-μm particle size, YMC).

    Techniques: Incubation, Purification, Chromatography