Structured Review

Waters Corporation semi preparative hplc
( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] <t>10a</t> from crude reaction mixture by semi-preparative <t>HPLC</t> (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .
Semi Preparative Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/semi preparative hplc/product/Waters Corporation
Average 94 stars, based on 84 article reviews
Price from $9.99 to $1999.99
semi preparative hplc - by Bioz Stars, 2020-11
94/100 stars

Images

1) Product Images from "A Promising PET Tracer for Imaging of α7 Nicotinic Acetylcholine Receptors in the Brain: Design, Synthesis, and in Vivo Evaluation of a Dibenzothiophene-Based Radioligand"

Article Title: A Promising PET Tracer for Imaging of α7 Nicotinic Acetylcholine Receptors in the Brain: Design, Synthesis, and in Vivo Evaluation of a Dibenzothiophene-Based Radioligand

Journal: Molecules

doi: 10.3390/molecules201018387

( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] 10a from crude reaction mixture by semi-preparative HPLC (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .
Figure Legend Snippet: ( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] 10a from crude reaction mixture by semi-preparative HPLC (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .

Techniques Used: Isolation, High Performance Liquid Chromatography, Flow Cytometry, Purification

2) Product Images from "Neuroprotective Effect of CR-777, a Glutathione Derivative of Withaferin A, Obtained through the Bioconversion of Withania somnifera (L.) Dunal Extract by the Fungus Beauveria bassiana"

Article Title: Neuroprotective Effect of CR-777, a Glutathione Derivative of Withaferin A, Obtained through the Bioconversion of Withania somnifera (L.) Dunal Extract by the Fungus Beauveria bassiana

Journal: Molecules

doi: 10.3390/molecules24244599

HPLC analysis during the incubation of withaferin A with Beauveria bassiana ATCC 7159. ( A ) Starting chromatogram, ( B ) after 5 days of incubation.
Figure Legend Snippet: HPLC analysis during the incubation of withaferin A with Beauveria bassiana ATCC 7159. ( A ) Starting chromatogram, ( B ) after 5 days of incubation.

Techniques Used: High Performance Liquid Chromatography, Incubation

3) Product Images from "Radiosynthesis and validation of (±)-[18F]-3-fluoro-2-hydroxypropionate ([18F]-FLac) as a PET tracer of lactate to monitor MCT1-dependent lactate uptake in tumors"

Article Title: Radiosynthesis and validation of (±)-[18F]-3-fluoro-2-hydroxypropionate ([18F]-FLac) as a PET tracer of lactate to monitor MCT1-dependent lactate uptake in tumors

Journal: Oncotarget

doi: 10.18632/oncotarget.14705

Synthesis of (±)-[ 18 F]-3-fluoro-2-hydroxypropionate ([ 18 F]-FLac) A . Scheme for the radiosynthesis of (±)-[ 18 F]-3-fluoro-2-hydroxypropionate. B . Co-elution spectra of (±)-[ 18 F]-benzyl 3-fluoro-2-hydroxypropionate and nonradioactive benzyl 3-fluoro-2-hydroxypropionate, and (±)-[ 18 F]-2-fluoro-3-hydroxybenzylacrylate and nonradioactive 2-fluoro-3-hydroxybenzylacrylate on a Supelco Discovery C18 HPLC column equipped with UV and NaI γ-ray detectors. C . Elution spectrum of (±)-[ 18 F]-3-fluoro-2-hydroxypropionate ([ 18 F]-FLac) on a IonPac AS15 Dionex HPLC column equipped with a NaI γ-ray detector.
Figure Legend Snippet: Synthesis of (±)-[ 18 F]-3-fluoro-2-hydroxypropionate ([ 18 F]-FLac) A . Scheme for the radiosynthesis of (±)-[ 18 F]-3-fluoro-2-hydroxypropionate. B . Co-elution spectra of (±)-[ 18 F]-benzyl 3-fluoro-2-hydroxypropionate and nonradioactive benzyl 3-fluoro-2-hydroxypropionate, and (±)-[ 18 F]-2-fluoro-3-hydroxybenzylacrylate and nonradioactive 2-fluoro-3-hydroxybenzylacrylate on a Supelco Discovery C18 HPLC column equipped with UV and NaI γ-ray detectors. C . Elution spectrum of (±)-[ 18 F]-3-fluoro-2-hydroxypropionate ([ 18 F]-FLac) on a IonPac AS15 Dionex HPLC column equipped with a NaI γ-ray detector.

Techniques Used: Co-Elution Assay, High Performance Liquid Chromatography

4) Product Images from "Radiosynthesis of the Tumor Hypoxia Marker [18F]TFMISO via O-[18F]Trifluoroethylation Reveals a Striking Difference Between Trifluoroethyl Tosylate and Iodide in Regiochemical Reactivity Toward Oxygen Nucleophiles"

Article Title: Radiosynthesis of the Tumor Hypoxia Marker [18F]TFMISO via O-[18F]Trifluoroethylation Reveals a Striking Difference Between Trifluoroethyl Tosylate and Iodide in Regiochemical Reactivity Toward Oxygen Nucleophiles

Journal: Bioorganic & medicinal chemistry

doi: 10.1016/j.bmc.2011.02.026

HPLC analysis of a reaction between 2,2,2-[ 18F ]trifluoroethly iodide and sodium glycidoxide (Approach B) co-injected with authentic TFMISO. The analytical HPLC was performed using a C18 column with a mobile phase of acetonitril and water (20/80) at a
Figure Legend Snippet: HPLC analysis of a reaction between 2,2,2-[ 18F ]trifluoroethly iodide and sodium glycidoxide (Approach B) co-injected with authentic TFMISO. The analytical HPLC was performed using a C18 column with a mobile phase of acetonitril and water (20/80) at a

Techniques Used: High Performance Liquid Chromatography, Injection

HPLC analysis of the reaction mixture co-injected with authentic TFMISO after removal of the OH-protection group. The analytical HPLC was performed using a C18 column and a mobile phase of acetonitrile and water (20/80) at a flow rate of 2 mL/min (for
Figure Legend Snippet: HPLC analysis of the reaction mixture co-injected with authentic TFMISO after removal of the OH-protection group. The analytical HPLC was performed using a C18 column and a mobile phase of acetonitrile and water (20/80) at a flow rate of 2 mL/min (for

Techniques Used: High Performance Liquid Chromatography, Injection, Flow Cytometry

5) Product Images from "Isolation and identification of induced systemic resistance determinants from Bacillus simplex Sneb545 against Heterodera glycines"

Article Title: Isolation and identification of induced systemic resistance determinants from Bacillus simplex Sneb545 against Heterodera glycines

Journal: Scientific Reports

doi: 10.1038/s41598-020-68548-4

Separation of S5 by HPLC. ( A ) HPLC spectrum of S5-1; ( B ) HPLC spectrum of S5-2; ( C ) HPLC spectrum of S5-3.
Figure Legend Snippet: Separation of S5 by HPLC. ( A ) HPLC spectrum of S5-1; ( B ) HPLC spectrum of S5-2; ( C ) HPLC spectrum of S5-3.

Techniques Used: High Performance Liquid Chromatography

6) Product Images from "Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: radiosynthesis and preclinical evaluation"

Article Title: Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: radiosynthesis and preclinical evaluation

Journal: Nuclear medicine and biology

doi: 10.1016/j.nucmedbio.2017.12.001

In vitro stability of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) at physiological pH was confirmed by HPLC at 2 hours ( A ) and 23 hours ( B ) after radiolabeling. The upper trace shows the radio-profile (red) and the lower trace shows the UV profile at 254 nm (green).(HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).
Figure Legend Snippet: In vitro stability of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) at physiological pH was confirmed by HPLC at 2 hours ( A ) and 23 hours ( B ) after radiolabeling. The upper trace shows the radio-profile (red) and the lower trace shows the UV profile at 254 nm (green).(HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).

Techniques Used: In Vitro, High Performance Liquid Chromatography, Radioactivity, Flow Cytometry

HPLC chromatograms of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ; top, red) co-injected with Re(CO) 3 (FEDA) ( 1 ; bottom, green) to confirm the radiotracer identity (HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).
Figure Legend Snippet: HPLC chromatograms of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ; top, red) co-injected with Re(CO) 3 (FEDA) ( 1 ; bottom, green) to confirm the radiotracer identity (HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).

Techniques Used: High Performance Liquid Chromatography, Injection, Flow Cytometry

Radio-HPLC chromatograms of purified Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) before injection ( A ) and of rat urine 10 min after i.v. injection of ( 18 F-1 ) ( B ] flow 1 mL/min).
Figure Legend Snippet: Radio-HPLC chromatograms of purified Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) before injection ( A ) and of rat urine 10 min after i.v. injection of ( 18 F-1 ) ( B ] flow 1 mL/min).

Techniques Used: High Performance Liquid Chromatography, Purification, Injection, Flow Cytometry

Related Articles

High Performance Liquid Chromatography:

Article Title: Neuroprotective Effect of CR-777, a Glutathione Derivative of Withaferin A, Obtained through the Bioconversion of Withania somnifera (L.) Dunal Extract by the Fungus Beauveria bassiana
Article Snippet: .. The withaferin A-enriched fraction (350 mg) was purified by semi-preparative HPLC on a reverse-phase column Sunfire III C18 (10 × 250 mm, 5 μm, Waters, Guyancourt, France), using an isocratic method with 38% acetonitrile. ..

Article Title: Radiosynthesis and validation of (±)-[18F]-3-fluoro-2-hydroxypropionate ([18F]-FLac) as a PET tracer of lactate to monitor MCT1-dependent lactate uptake in tumors
Article Snippet: .. The (±)-[18 F]-3-fluoro-2-hydroxypropionate precursor was isolated by semi-preparative HPLC (20 mM NaH2 PO4 /CH3 CN 70/30, 3 mL/min, retention time = 21 min), diluted with water (1.5 x vol), and loaded on a conditioned C18 cartridge (Waters). ..

Article Title: A Promising PET Tracer for Imaging of α7 Nicotinic Acetylcholine Receptors in the Brain: Design, Synthesis, and in Vivo Evaluation of a Dibenzothiophene-Based Radioligand
Article Snippet: .. After isolation via semi-preparative HPLC, [18 F] 10a was trapped on a pre-conditioned Sep-Pak® (Waters, Milford, MA, USA) C18 light cartridge, and formulated in isotonic saline containing 10% of EtOH (v /v ). ..

Article Title: Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: radiosynthesis and preclinical evaluation
Article Snippet: .. The crude radiotracer Re(CO)3 ([18 F]FEDA) (18 F-1 ) was purified by semi-preparative HPLC and the desired pure radioactive fractions were combined. ..

Article Title: Isolation and identification of induced systemic resistance determinants from Bacillus simplex Sneb545 against Heterodera glycines
Article Snippet: .. The chromatographic conditions were similar to those described in “Separation of S5 through semi-preparative HPLC” with some modifications, such as the mobile phase of acetonitrile: water was 10:90 (v: v). .. The eluate was collected according to the absorption peak and the purity was assessed by analytical HPLC.

Article Title: Radiosynthesis of the Tumor Hypoxia Marker [18F]TFMISO via O-[18F]Trifluoroethylation Reveals a Striking Difference Between Trifluoroethyl Tosylate and Iodide in Regiochemical Reactivity Toward Oxygen Nucleophiles
Article Snippet: .. The 18 F-labeled product which eluted at the retention time corresponding to that of authentic TFMISO was isolated by semi-preparative HPLC, concentrated by solid phase extraction using a tC18 SepPak Plus cartridge (Waters) and eluted with 2 mL of methanol and the solvent evaporated. .. The final product was confirmed as [18 F]TFMISO by HPLC and mass spectrometry.

Article Title: Rapid identification of triterpenoid sulfates and hydroxy fatty acids including two new constituents from Tydemania expeditionis by LC-MS
Article Snippet: .. Semi-preparative HPLC was performed on a Waters 2695 system equipped with 2996 diode-array UV detection. .. Mixture of compounds 5-6 (retention time 42.2-43.0 min) was purified using an Agilent Zorbax SB-C18 reversed-phase column (5 μm, 9.4 × 250 mm) utilizing the same gradient of aqueous acetonitrile as that used in the LC-MS analysis with detection at 215 nm.

Article Title: Cellular uptake of cyclotide MCoTI-I follows multiple endocytic pathways
Article Snippet: .. Preparative and semi-preparative HPLC were performed on a Waters Delta Prep system fitted with a Waters 2487 UV-visible detector using a Vydac C18 (15–20 μm, 10 × 250 mm) at a flow rate of 5 mL/min. ..

Isolation:

Article Title: Radiosynthesis and validation of (±)-[18F]-3-fluoro-2-hydroxypropionate ([18F]-FLac) as a PET tracer of lactate to monitor MCT1-dependent lactate uptake in tumors
Article Snippet: .. The (±)-[18 F]-3-fluoro-2-hydroxypropionate precursor was isolated by semi-preparative HPLC (20 mM NaH2 PO4 /CH3 CN 70/30, 3 mL/min, retention time = 21 min), diluted with water (1.5 x vol), and loaded on a conditioned C18 cartridge (Waters). ..

Article Title: A Promising PET Tracer for Imaging of α7 Nicotinic Acetylcholine Receptors in the Brain: Design, Synthesis, and in Vivo Evaluation of a Dibenzothiophene-Based Radioligand
Article Snippet: .. After isolation via semi-preparative HPLC, [18 F] 10a was trapped on a pre-conditioned Sep-Pak® (Waters, Milford, MA, USA) C18 light cartridge, and formulated in isotonic saline containing 10% of EtOH (v /v ). ..

Article Title: Radiosynthesis of the Tumor Hypoxia Marker [18F]TFMISO via O-[18F]Trifluoroethylation Reveals a Striking Difference Between Trifluoroethyl Tosylate and Iodide in Regiochemical Reactivity Toward Oxygen Nucleophiles
Article Snippet: .. The 18 F-labeled product which eluted at the retention time corresponding to that of authentic TFMISO was isolated by semi-preparative HPLC, concentrated by solid phase extraction using a tC18 SepPak Plus cartridge (Waters) and eluted with 2 mL of methanol and the solvent evaporated. .. The final product was confirmed as [18 F]TFMISO by HPLC and mass spectrometry.

Flow Cytometry:

Article Title: Cellular uptake of cyclotide MCoTI-I follows multiple endocytic pathways
Article Snippet: .. Preparative and semi-preparative HPLC were performed on a Waters Delta Prep system fitted with a Waters 2487 UV-visible detector using a Vydac C18 (15–20 μm, 10 × 250 mm) at a flow rate of 5 mL/min. ..

Purification:

Article Title: Neuroprotective Effect of CR-777, a Glutathione Derivative of Withaferin A, Obtained through the Bioconversion of Withania somnifera (L.) Dunal Extract by the Fungus Beauveria bassiana
Article Snippet: .. The withaferin A-enriched fraction (350 mg) was purified by semi-preparative HPLC on a reverse-phase column Sunfire III C18 (10 × 250 mm, 5 μm, Waters, Guyancourt, France), using an isocratic method with 38% acetonitrile. ..

Article Title: Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: radiosynthesis and preclinical evaluation
Article Snippet: .. The crude radiotracer Re(CO)3 ([18 F]FEDA) (18 F-1 ) was purified by semi-preparative HPLC and the desired pure radioactive fractions were combined. ..

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    Waters Corporation semi preparative hplc
    ( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] <t>10a</t> from crude reaction mixture by semi-preparative <t>HPLC</t> (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .
    Semi Preparative Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative hplc/product/Waters Corporation
    Average 94 stars, based on 84 article reviews
    Price from $9.99 to $1999.99
    semi preparative hplc - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

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    ( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] 10a from crude reaction mixture by semi-preparative HPLC (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .

    Journal: Molecules

    Article Title: A Promising PET Tracer for Imaging of α7 Nicotinic Acetylcholine Receptors in the Brain: Design, Synthesis, and in Vivo Evaluation of a Dibenzothiophene-Based Radioligand

    doi: 10.3390/molecules201018387

    Figure Lengend Snippet: ( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] 10a from crude reaction mixture by semi-preparative HPLC (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .

    Article Snippet: After isolation via semi-preparative HPLC, [18 F] 10a was trapped on a pre-conditioned Sep-Pak® (Waters, Milford, MA, USA) C18 light cartridge, and formulated in isotonic saline containing 10% of EtOH (v /v ).

    Techniques: Isolation, High Performance Liquid Chromatography, Flow Cytometry, Purification