Structured Review

Phenomenex semi preparative hplc
<t>RP-HPLC</t> chromatograms of extract after hydrolysis: ( 1 ) p -coumaric acid glucoside (CoAG); ( 2 ) ferulic acid glucoside (FeAG); ( 3 ) <t>SDG;</t> ( 4 ) p -coumaric acid; and ( 5 ) ferulic acid.
Semi Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 92/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Antioxidant Activity of Flaxseed Extracts in Lipid Systems"

Article Title: Antioxidant Activity of Flaxseed Extracts in Lipid Systems

Journal: Molecules

doi: 10.3390/molecules21010017

RP-HPLC chromatograms of extract after hydrolysis: ( 1 ) p -coumaric acid glucoside (CoAG); ( 2 ) ferulic acid glucoside (FeAG); ( 3 ) SDG; ( 4 ) p -coumaric acid; and ( 5 ) ferulic acid.
Figure Legend Snippet: RP-HPLC chromatograms of extract after hydrolysis: ( 1 ) p -coumaric acid glucoside (CoAG); ( 2 ) ferulic acid glucoside (FeAG); ( 3 ) SDG; ( 4 ) p -coumaric acid; and ( 5 ) ferulic acid.

Techniques Used: High Performance Liquid Chromatography

2) Product Images from "Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum"

Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules22061003

Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.
Figure Legend Snippet: Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry, Injection

3) Product Images from "Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model"

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

Journal: Phytotherapy Research

doi: 10.1002/ptr.6767

Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Figure Legend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

Techniques Used: High Performance Liquid Chromatography

4) Product Images from "Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor"

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor

Journal: Nature protocols

doi: 10.1038/s41596-020-0305-9

Analytical HPLC trace of [ 18 F]FDOPA using a Luna NH 2 with permission from The Royal Society of Chemistry.
Figure Legend Snippet: Analytical HPLC trace of [ 18 F]FDOPA using a Luna NH 2 with permission from The Royal Society of Chemistry.

Techniques Used: High Performance Liquid Chromatography

5) Product Images from "Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model"

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

Journal: Phytotherapy Research

doi: 10.1002/ptr.6767

Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Figure Legend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

Techniques Used: High Performance Liquid Chromatography

6) Product Images from "Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods"

Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

Journal: Carbohydrate research

doi: 10.1016/j.carres.2011.09.020

Adaptation of RPIP-HPLC method developed here to separate sulfonated products from reaction mixtures by gravity flow benchtop C18 resin. N -cbz O -sulfonated neomycin was eluted in 10 mM ammonium acetate, initially in 100% water, and then with addition
Figure Legend Snippet: Adaptation of RPIP-HPLC method developed here to separate sulfonated products from reaction mixtures by gravity flow benchtop C18 resin. N -cbz O -sulfonated neomycin was eluted in 10 mM ammonium acetate, initially in 100% water, and then with addition

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

7) Product Images from "Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods"

Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

Journal: Carbohydrate research

doi: 10.1016/j.carres.2011.09.020

RPIP-HPLC-MS total ion chromatograms (TICs) of reaction products from the sulfonation of N -cbz aminoglycosides. Shown are product mixtures from the sulfonation of kanamycin and apramycin with Pyr·SO 3 (A and B, respectively) and the sulfonation
Figure Legend Snippet: RPIP-HPLC-MS total ion chromatograms (TICs) of reaction products from the sulfonation of N -cbz aminoglycosides. Shown are product mixtures from the sulfonation of kanamycin and apramycin with Pyr·SO 3 (A and B, respectively) and the sulfonation

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

Representative comparison of analytical HPLC chromatograms from various RPIP conditions eluting persulfated N -cbz neomycin. Each chromatogram was generated from a 20 min. gradient increase in percent ACN, and the ending percent ACN was then maintained
Figure Legend Snippet: Representative comparison of analytical HPLC chromatograms from various RPIP conditions eluting persulfated N -cbz neomycin. Each chromatogram was generated from a 20 min. gradient increase in percent ACN, and the ending percent ACN was then maintained

Techniques Used: High Performance Liquid Chromatography, Generated

Adaptation of RPIP-HPLC method developed here to separate sulfonated products from reaction mixtures by gravity flow benchtop C18 resin. N -cbz O -sulfonated neomycin was eluted in 10 mM ammonium acetate, initially in 100% water, and then with addition
Figure Legend Snippet: Adaptation of RPIP-HPLC method developed here to separate sulfonated products from reaction mixtures by gravity flow benchtop C18 resin. N -cbz O -sulfonated neomycin was eluted in 10 mM ammonium acetate, initially in 100% water, and then with addition

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

Separation of N -cbz O -sulfonated kanamycin derivatives that differ by degree of sulfation and the position of sulfate groups using RPIP-HPLC interfaced with an ESI- ion trap mass analyzer. Peaks for all sulfonation products are shown in the total ion
Figure Legend Snippet: Separation of N -cbz O -sulfonated kanamycin derivatives that differ by degree of sulfation and the position of sulfate groups using RPIP-HPLC interfaced with an ESI- ion trap mass analyzer. Peaks for all sulfonation products are shown in the total ion

Techniques Used: High Performance Liquid Chromatography

RPIP-HPLC to follow reaction progression over time for O -sulfonation of N -cbz neomycin. Following addition of ClSO 3 H, reaction aliquots were analyzed at: (A) 5 min, (B) 15 min, (C) 20 min, (D) 50 min, (E) 275 min. Percent of reaction product that is persulfated
Figure Legend Snippet: RPIP-HPLC to follow reaction progression over time for O -sulfonation of N -cbz neomycin. Following addition of ClSO 3 H, reaction aliquots were analyzed at: (A) 5 min, (B) 15 min, (C) 20 min, (D) 50 min, (E) 275 min. Percent of reaction product that is persulfated

Techniques Used: High Performance Liquid Chromatography

Sulfonation of N -cbz aminoglycosides (kanamycin, apramycin, and neomycin) with Pyr·SO 3 or ClSO 3 H resulted in products with varied degrees of sulfation (DS). RPIP-HPLC methods developed here were used to separate reaction products based on degree
Figure Legend Snippet: Sulfonation of N -cbz aminoglycosides (kanamycin, apramycin, and neomycin) with Pyr·SO 3 or ClSO 3 H resulted in products with varied degrees of sulfation (DS). RPIP-HPLC methods developed here were used to separate reaction products based on degree

Techniques Used: High Performance Liquid Chromatography

8) Product Images from "Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor"

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor

Journal: Nature protocols

doi: 10.1038/s41596-020-0305-9

Semi-preparative HPLC traces for [ 18 with permission from The Royal Society of Chemistry), and B: the alternate one-pot method with HLB purification between fluorination and deprotection
Figure Legend Snippet: Semi-preparative HPLC traces for [ 18 with permission from The Royal Society of Chemistry), and B: the alternate one-pot method with HLB purification between fluorination and deprotection

Techniques Used: High Performance Liquid Chromatography, Purification

9) Product Images from "Unusual Secondary Metabolites of the Aerial Parts of Dionysia diapensifolia Bioss. (Primulaceae) and Their Anti-Inflammatory Activity"

Article Title: Unusual Secondary Metabolites of the Aerial Parts of Dionysia diapensifolia Bioss. (Primulaceae) and Their Anti-Inflammatory Activity

Journal: Biomolecules

doi: 10.3390/biom10030438

HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: Phenomenex Synergi Max-RP C18 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.
Figure Legend Snippet: HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: Phenomenex Synergi Max-RP C18 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay, Injection

Chromatogram of the HPLC-DAD analysis of the diethyl ether subfraction ( A ) and an enlarged view ( B ) at λ 254 nm obtained from the methanolic extract of the aerial parts of D. diapensifolia . Analysis conditions: stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 8 mg/mL, 10 µL.
Figure Legend Snippet: Chromatogram of the HPLC-DAD analysis of the diethyl ether subfraction ( A ) and an enlarged view ( B ) at λ 254 nm obtained from the methanolic extract of the aerial parts of D. diapensifolia . Analysis conditions: stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 8 mg/mL, 10 µL.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay, Injection

10) Product Images from "Anti-Human Rhinoviral Activity of Polybromocatechol Compounds Isolated from the Rhodophyta, Neorhodomela aculeata"

Article Title: Anti-Human Rhinoviral Activity of Polybromocatechol Compounds Isolated from the Rhodophyta, Neorhodomela aculeata

Journal: Marine Drugs

doi: 10.3390/md10102222

RP-HPLC profile of sub-fractions of EtOAc-soluble fraction (ESF) of N. aculeata . Performed on an Agilent 1300 HPLC system fitted with a Phenomenex Luna C18 (2) column (150 × 4.6 mm, 5 μm). The elution solvent system was binary gradient of solvent A (0.02% trifluoroacetic acid (TFA) in water); solvent B (0.02% TFA in acetonitrile). The gradient flow program was, as follows: 0 min, 10% B; 30 min. The flow rate was 0.7 mL/min and detection wavelength was set at 280 nm and column temperature was 25 °C. The chromatogram of F1 was not shown.
Figure Legend Snippet: RP-HPLC profile of sub-fractions of EtOAc-soluble fraction (ESF) of N. aculeata . Performed on an Agilent 1300 HPLC system fitted with a Phenomenex Luna C18 (2) column (150 × 4.6 mm, 5 μm). The elution solvent system was binary gradient of solvent A (0.02% trifluoroacetic acid (TFA) in water); solvent B (0.02% TFA in acetonitrile). The gradient flow program was, as follows: 0 min, 10% B; 30 min. The flow rate was 0.7 mL/min and detection wavelength was set at 280 nm and column temperature was 25 °C. The chromatogram of F1 was not shown.

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

11) Product Images from "Characterization of the flavoenzyme XiaK as an N-hydroxylase and implications in indolosesquiterpene diversification and implications in indolosesquiterpene diversification †Electronic supplementary information (ESI) available: The experimental procedures, materials, and characterization of compounds. See DOI: 10.1039/c7sc01182bClick here for additional data file."

Article Title: Characterization of the flavoenzyme XiaK as an N-hydroxylase and implications in indolosesquiterpene diversification and implications in indolosesquiterpene diversification †Electronic supplementary information (ESI) available: The experimental procedures, materials, and characterization of compounds. See DOI: 10.1039/c7sc01182bClick here for additional data file.

Journal: Chemical Science

doi: 10.1039/c7sc01182b

Time course of the XiaK reaction and stabilities of XiaK products. (A) HPLC analysis of the time course assay of an in vitro XiaK reaction: (i) 0 min (before adding XiaK); (ii) 5 min; (iii) 10 min; (iv) 15 min; (v) 20 min; (vi) 30 min; (vii) 1 h; (viii) 2 h; (ix) 4 h; (x) 8 h; (xi) 24 h; (xii) 5 days; (xiii) 12 days; (xiv) 1 and 17 standards. (B) Chemical structures of XMA analogues. (C) Stability of 12 dissolved in 50 mM Na 2 HPO 4 –NaH 2 PO 4 buffer (pH 8.0): (xv) –20 °C for 2 days; (xvi) –20 °C for 4 days; (xvii) room temperature (RT) for 2 days; (xviii) RT for 4 days; (xix) 60 °C for 2 days; (xx) 60 °C for 4 days; (xxi) XMA ( 1 ) standard. The filled black circles () denote multiple XMA-related products which were not isolated for structure elucidation. (D) Stability of OXM ( 7 ) treated under various conditions: (xxii) OXM ( 7 ) dissolved in 50 mM Na 2 HPO 4 –NaH 2 PO 4 buffer (pH 8.0) and incubated at 60 °C for 4 days; (xxiii) 7 dissolved in H 2 O/MeCN (1 : 1, v/v) and incubated at 60 °C for 4 days; (xxiv) 7 dissolved in MeOH and incubated at 60 °C for 4 days. (E) Stability of 16 : (xxv) a complete XiaK assay at 28 °C for 6 h; (xxvi) 16 isolated from an analytical XiaK assay (in trace xxv) immediately used for HPLC analysis; (xxvii) NOXM ( 12 ) isolated from an analytical XiaK assay (in trace xxv) immediately used for HPLC analysis.
Figure Legend Snippet: Time course of the XiaK reaction and stabilities of XiaK products. (A) HPLC analysis of the time course assay of an in vitro XiaK reaction: (i) 0 min (before adding XiaK); (ii) 5 min; (iii) 10 min; (iv) 15 min; (v) 20 min; (vi) 30 min; (vii) 1 h; (viii) 2 h; (ix) 4 h; (x) 8 h; (xi) 24 h; (xii) 5 days; (xiii) 12 days; (xiv) 1 and 17 standards. (B) Chemical structures of XMA analogues. (C) Stability of 12 dissolved in 50 mM Na 2 HPO 4 –NaH 2 PO 4 buffer (pH 8.0): (xv) –20 °C for 2 days; (xvi) –20 °C for 4 days; (xvii) room temperature (RT) for 2 days; (xviii) RT for 4 days; (xix) 60 °C for 2 days; (xx) 60 °C for 4 days; (xxi) XMA ( 1 ) standard. The filled black circles () denote multiple XMA-related products which were not isolated for structure elucidation. (D) Stability of OXM ( 7 ) treated under various conditions: (xxii) OXM ( 7 ) dissolved in 50 mM Na 2 HPO 4 –NaH 2 PO 4 buffer (pH 8.0) and incubated at 60 °C for 4 days; (xxiii) 7 dissolved in H 2 O/MeCN (1 : 1, v/v) and incubated at 60 °C for 4 days; (xxiv) 7 dissolved in MeOH and incubated at 60 °C for 4 days. (E) Stability of 16 : (xxv) a complete XiaK assay at 28 °C for 6 h; (xxvi) 16 isolated from an analytical XiaK assay (in trace xxv) immediately used for HPLC analysis; (xxvii) NOXM ( 12 ) isolated from an analytical XiaK assay (in trace xxv) immediately used for HPLC analysis.

Techniques Used: High Performance Liquid Chromatography, In Vitro, Isolation, Incubation

12) Product Images from "Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model"

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

Journal: Phytotherapy Research

doi: 10.1002/ptr.6767

Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Figure Legend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

Techniques Used: High Performance Liquid Chromatography

13) Product Images from "Unusual Secondary Metabolites of the Aerial Parts of Dionysia diapensifolia Bioss. (Primulaceae) and Their Anti-Inflammatory Activity"

Article Title: Unusual Secondary Metabolites of the Aerial Parts of Dionysia diapensifolia Bioss. (Primulaceae) and Their Anti-Inflammatory Activity

Journal: Biomolecules

doi: 10.3390/biom10030438

HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: Phenomenex Synergi Max-RP C18 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.
Figure Legend Snippet: HPLC-DAD chromatogram of methanolic extract of the aerial parts of D. diapensifolia . Analysis condition: stationary phase: Phenomenex Synergi Max-RP C18 4 µm, 150 × 4.6 mm; mobile Phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 98%; 35 min: B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 2 mg/mL, 10 µL; detection = 254 nm.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay, Injection

Chromatogram of the HPLC-DAD analysis of the diethyl ether subfraction ( A ) and an enlarged view ( B ) at λ 254 nm obtained from the methanolic extract of the aerial parts of D. diapensifolia . Analysis conditions: stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 8 mg/mL, 10 µL.
Figure Legend Snippet: Chromatogram of the HPLC-DAD analysis of the diethyl ether subfraction ( A ) and an enlarged view ( B ) at λ 254 nm obtained from the methanolic extract of the aerial parts of D. diapensifolia . Analysis conditions: stationary phase: Phenomenex Aqua C18 5 µm, 250 × 4.6 mm; mobile phase: A = H 2 O + 0.02% TFA, B = acetonitrile; gradient: 0 min: B = 2%; 20 min: B = 50%; 40 min: B = 98%; 50 min B = 98%; T: 35 °C; flow: 1 mL/min; sample concentration and injection volume: 8 mg/mL, 10 µL.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay, Injection

14) Product Images from "Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor"

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor

Journal: Nature protocols

doi: 10.1038/s41596-020-0305-9

Analytical HPLC trace of [ 18 F]FDOPA using a Luna NH 2 with permission from The Royal Society of Chemistry.
Figure Legend Snippet: Analytical HPLC trace of [ 18 F]FDOPA using a Luna NH 2 with permission from The Royal Society of Chemistry.

Techniques Used: High Performance Liquid Chromatography

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Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods
Article Snippet: .. In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC. .. To this end, the product mixture was dissolved in 3:1 ACN:water and replicate aliquots injected and separated.

Article Title: Antioxidant Activity of Flaxseed Extracts in Lipid Systems
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Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods
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Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum
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Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Compound 10 (3.0 mg, 79.2% area) was purified from fraction C6‐11 (30.1 mg) by semi‐preparative HPLC (Phenomenex Luna 5 μm C8, 150 × 21.2 mm, 80% CH3 CN) at 27 min. ..

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor
Article Snippet: .. Following deprotection, the entire reaction mixture is then injected onto the column for purification of [18 F]FDOPA by semi-preparative HPLC. ..

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Compound 4 (3.4 mg, 86.8% area) and 6 (1.5 mg, 90.0% area) were purified from fraction H15‐2 (23 mg) by semi‐preparative HPLC (Phenomenex Luna 5 μm C18, 250 × 21.2 mm, 35% CH3CN) at 42 and 47 min. ..

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor
Article Snippet: .. This pre-purification requires a modified TRACERlab FXFN synthesis module (see for configuration and for timelist), and has two benefits: 1) it removes most of the copper, potentially reducing the extent of oxidative [18 F]FDOPA degradation during deprotection, and 2) it removes N-N -dimethylformamide (DMF), which simplifies semi-preparative HPLC. ..

Modification:

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor
Article Snippet: .. This pre-purification requires a modified TRACERlab FXFN synthesis module (see for configuration and for timelist), and has two benefits: 1) it removes most of the copper, potentially reducing the extent of oxidative [18 F]FDOPA degradation during deprotection, and 2) it removes N-N -dimethylformamide (DMF), which simplifies semi-preparative HPLC. ..

Flow Cytometry:

Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum
Article Snippet: .. A fraction eluted with 300–980 mL (53.89 mg) was further purified by semi-preparative HPLC (stationary phase: Phenomenex Aqua C18 column (5 µm; 250 × 10 mm); mobile phase: solvent A: water solvent B: acetonitrile, gradient elution: 0 min: 45% A; 16 min: 2% A; 21 min: 45% A; 30 min: stop; flow 2.00 mL/min; 40 °C; 150 µL injection volume of a solution of 18.00 mg/mL methanol). ..

Injection:

Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum
Article Snippet: .. A fraction eluted with 300–980 mL (53.89 mg) was further purified by semi-preparative HPLC (stationary phase: Phenomenex Aqua C18 column (5 µm; 250 × 10 mm); mobile phase: solvent A: water solvent B: acetonitrile, gradient elution: 0 min: 45% A; 16 min: 2% A; 21 min: 45% A; 30 min: stop; flow 2.00 mL/min; 40 °C; 150 µL injection volume of a solution of 18.00 mg/mL methanol). ..

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor
Article Snippet: .. Following deprotection, the entire reaction mixture is then injected onto the column for purification of [18 F]FDOPA by semi-preparative HPLC. ..

Purification:

Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods
Article Snippet: .. In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC. .. To this end, the product mixture was dissolved in 3:1 ACN:water and replicate aliquots injected and separated.

Article Title: Antioxidant Activity of Flaxseed Extracts in Lipid Systems
Article Snippet: .. From this fraction, pure SDG was purified using semi-preparative HPLC on a Luna C18 (250 × 10 mm, 5 μm; Phenomenex) column. .. A flow rate of 3 mL/min and the same gradient elution as by an analytical HPLC were used.

Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum
Article Snippet: .. A fraction eluted with 300–980 mL (53.89 mg) was further purified by semi-preparative HPLC (stationary phase: Phenomenex Aqua C18 column (5 µm; 250 × 10 mm); mobile phase: solvent A: water solvent B: acetonitrile, gradient elution: 0 min: 45% A; 16 min: 2% A; 21 min: 45% A; 30 min: stop; flow 2.00 mL/min; 40 °C; 150 µL injection volume of a solution of 18.00 mg/mL methanol). ..

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Compound 10 (3.0 mg, 79.2% area) was purified from fraction C6‐11 (30.1 mg) by semi‐preparative HPLC (Phenomenex Luna 5 μm C8, 150 × 21.2 mm, 80% CH3 CN) at 27 min. ..

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor
Article Snippet: .. Following deprotection, the entire reaction mixture is then injected onto the column for purification of [18 F]FDOPA by semi-preparative HPLC. ..

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Compound 4 (3.4 mg, 86.8% area) and 6 (1.5 mg, 90.0% area) were purified from fraction H15‐2 (23 mg) by semi‐preparative HPLC (Phenomenex Luna 5 μm C18, 250 × 21.2 mm, 35% CH3CN) at 42 and 47 min. ..

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    Phenomenex semi preparative hplc
    <t>RP-HPLC</t> chromatograms of extract after hydrolysis: ( 1 ) p -coumaric acid glucoside (CoAG); ( 2 ) ferulic acid glucoside (FeAG); ( 3 ) <t>SDG;</t> ( 4 ) p -coumaric acid; and ( 5 ) ferulic acid.
    Semi Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 92/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RP-HPLC chromatograms of extract after hydrolysis: ( 1 ) p -coumaric acid glucoside (CoAG); ( 2 ) ferulic acid glucoside (FeAG); ( 3 ) SDG; ( 4 ) p -coumaric acid; and ( 5 ) ferulic acid.

    Journal: Molecules

    Article Title: Antioxidant Activity of Flaxseed Extracts in Lipid Systems

    doi: 10.3390/molecules21010017

    Figure Lengend Snippet: RP-HPLC chromatograms of extract after hydrolysis: ( 1 ) p -coumaric acid glucoside (CoAG); ( 2 ) ferulic acid glucoside (FeAG); ( 3 ) SDG; ( 4 ) p -coumaric acid; and ( 5 ) ferulic acid.

    Article Snippet: From this fraction, pure SDG was purified using semi-preparative HPLC on a Luna C18 (250 × 10 mm, 5 μm; Phenomenex) column.

    Techniques: High Performance Liquid Chromatography

    Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum

    doi: 10.3390/molecules22061003

    Figure Lengend Snippet: Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.

    Article Snippet: A fraction eluted with 300–980 mL (53.89 mg) was further purified by semi-preparative HPLC (stationary phase: Phenomenex Aqua C18 column (5 µm; 250 × 10 mm); mobile phase: solvent A: water solvent B: acetonitrile, gradient elution: 0 min: 45% A; 16 min: 2% A; 21 min: 45% A; 30 min: stop; flow 2.00 mL/min; 40 °C; 150 µL injection volume of a solution of 18.00 mg/mL methanol).

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection

    Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

    Journal: Phytotherapy Research

    Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

    doi: 10.1002/ptr.6767

    Figure Lengend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

    Article Snippet: Compound 4 (3.4 mg, 86.8% area) and 6 (1.5 mg, 90.0% area) were purified from fraction H15‐2 (23 mg) by semi‐preparative HPLC (Phenomenex Luna 5 μm C18, 250 × 21.2 mm, 35% CH3CN) at 42 and 47 min.

    Techniques: High Performance Liquid Chromatography

    Analytical HPLC trace of [ 18 F]FDOPA using a Luna NH 2 with permission from The Royal Society of Chemistry.

    Journal: Nature protocols

    Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor

    doi: 10.1038/s41596-020-0305-9

    Figure Lengend Snippet: Analytical HPLC trace of [ 18 F]FDOPA using a Luna NH 2 with permission from The Royal Society of Chemistry.

    Article Snippet: Following deprotection, the entire reaction mixture is then injected onto the column for purification of [18 F]FDOPA by semi-preparative HPLC.

    Techniques: High Performance Liquid Chromatography