Structured Review

Phenomenex semi preparative hplc column
<t>HPLC</t> chromatograms (system 1) of reaction mixtures of <t>DOTA-SP(4–11),</t> DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.
Semi Preparative Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/semi preparative hplc column/product/Phenomenex
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
semi preparative hplc column - by Bioz Stars, 2021-05
86/100 stars

Images

1) Product Images from "Application of Neurokinin-1 Receptor in Targeted Strategies for Glioma Treatment. Part I: Synthesis and Evaluation of Substance P Fragments Labeled with 99mTc and 177Lu as Potential Receptor Radiopharmaceuticals"

Article Title: Application of Neurokinin-1 Receptor in Targeted Strategies for Glioma Treatment. Part I: Synthesis and Evaluation of Substance P Fragments Labeled with 99mTc and 177Lu as Potential Receptor Radiopharmaceuticals

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules23102542

HPLC chromatograms (system 1) of reaction mixtures of DOTA-SP(4–11), DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.
Figure Legend Snippet: HPLC chromatograms (system 1) of reaction mixtures of DOTA-SP(4–11), DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.

Techniques Used: High Performance Liquid Chromatography

2) Product Images from "Application of Neurokinin-1 Receptor in Targeted Strategies for Glioma Treatment. Part I: Synthesis and Evaluation of Substance P Fragments Labeled with 99mTc and 177Lu as Potential Receptor Radiopharmaceuticals"

Article Title: Application of Neurokinin-1 Receptor in Targeted Strategies for Glioma Treatment. Part I: Synthesis and Evaluation of Substance P Fragments Labeled with 99mTc and 177Lu as Potential Receptor Radiopharmaceuticals

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules23102542

HPLC chromatograms (system 1) of reaction mixtures of DOTA-SP(4–11), DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.
Figure Legend Snippet: HPLC chromatograms (system 1) of reaction mixtures of DOTA-SP(4–11), DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.

Techniques Used: High Performance Liquid Chromatography

3) Product Images from "Isolation and Rationale for the Formation of Isomeric Decarbamoylmitomycin C-N6-deoxyadenosine Adducts in DNA"

Article Title: Isolation and Rationale for the Formation of Isomeric Decarbamoylmitomycin C-N6-deoxyadenosine Adducts in DNA

Journal: Chemical research in toxicology

doi: 10.1021/acs.chemrestox.8b00102

HPLC chromatograms of enzymatic digests of DMC-oligonucleotide complexes formed under bifunctional conditions with duplex oligonucleotides 22 (a), 23 (b) and 24 (c) which have no end-chain dAs. Adducts 5a and 5b could not be detected.
Figure Legend Snippet: HPLC chromatograms of enzymatic digests of DMC-oligonucleotide complexes formed under bifunctional conditions with duplex oligonucleotides 22 (a), 23 (b) and 24 (c) which have no end-chain dAs. Adducts 5a and 5b could not be detected.

Techniques Used: High Performance Liquid Chromatography

Representative HPLC chromatograms of enzymatic digests of DMC-oligonucleotide complexes formed under bifunctional conditions oligonucleotides 6 (a), 8 (b), 25 (c), 19 (d), 9 (e), 28 (f).
Figure Legend Snippet: Representative HPLC chromatograms of enzymatic digests of DMC-oligonucleotide complexes formed under bifunctional conditions oligonucleotides 6 (a), 8 (b), 25 (c), 19 (d), 9 (e), 28 (f).

Techniques Used: High Performance Liquid Chromatography

Related Articles

High Performance Liquid Chromatography:

Article Title: Detecting the interaction of peptide ligands with plant membrane receptors
Article Snippet: Identification of HPLC peaks are determined by mass-to-charge ratios (m/z) which depend on peptide sequence. .. Changing this solution between vials may slightly alter the retention time of peptides on the semi-preparative HPLC column. .. In extreme cases of insoluble peptides, it is advisable to consider purchasing a dedicated C-12 semi-preparative column so that other purified peptides are not contaminated with ligand probes.

Article Title: Preparation of the Metabotropic Glutamate Receptor 5 (mGluR5) PET Tracer [18F]FPEB for Human Use: An Automated Radiosynthesis and a Novel One-Pot Synthesis of its Radiolabeling Precursor
Article Snippet: During this process, the [18 F]FPEB crude product was thoroughly mixed with the pre-filled water (2.5 mL) in receiving vial 13 by argon flow. .. The crude [18 F]FPEB product mixture in receiving vial 13 was loaded into a 5 mL stainless steel loop and injected onto the semi-preparative HPLC COLUMN . .. The chromatogram was monitored with tandem radioactivity and UV (absorbance at 254 nm) detectors.

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor
Article Snippet: Install HPLC eluents on the TRACERLab system (Reservoir 1: 90% MeCN; Reservoir 2: 75% MeCN). .. Attach a guard column to the semi-preparative HPLC column and install on the TRACERLab module. ..

Article Title: Application of Neurokinin-1 Receptor in Targeted Strategies for Glioma Treatment. Part I: Synthesis and Evaluation of Substance P Fragments Labeled with 99mTc and 177Lu as Potential Receptor Radiopharmaceuticals
Article Snippet: .. Crude CN-SP products ( ) were purified on a semi-preparative HPLC column (system 1; Phenomenex, Torrance, CA, USA) and lyophilized (yield ≈85–95%). .. MS of CN-SP(1–11): m/z : calc.

Article Title: Preparation of the Metabotropic Glutamate Receptor 5 (mGluR5) PET Tracer [18F]FPEB for Human Use: An Automated Radiosynthesis and a Novel One-Pot Synthesis of its Radiolabeling Precursor
Article Snippet: A 10 mL sterile empty vial ( DOSE VIAL ) attached with a Millipore Millex® GV membrane filter (0.22 µm, 33 mm) and a sterile venting needle was connected to the end of product line from valve v16 . .. A semi-preparative HPLC COLUMN was put in place and equilibrated with the mobile phase for 25 min at a flow rate of 5 mL/min before use. ..

Article Title: Synthesis of High Molar Activity [18F]6-Fluoro-L-DOPA Suitable for Human Use by Cu-Mediated Fluorination of a BPin Precursor
Article Snippet: .. Preparation of semi-preparative (Luna NH2 5μ 10 × 250 mm) HPLC column: Assemble guard column as per manufacturer instructions and attach to semi-preparative HPLC column. .. Flush column for 15 – 30 min at 5 mL/min with 90% MeCN (Semi-preparative HPLC eluent #1) prior to installation in the synthesis module.

Injection:

Article Title: Preparation of the Metabotropic Glutamate Receptor 5 (mGluR5) PET Tracer [18F]FPEB for Human Use: An Automated Radiosynthesis and a Novel One-Pot Synthesis of its Radiolabeling Precursor
Article Snippet: During this process, the [18 F]FPEB crude product was thoroughly mixed with the pre-filled water (2.5 mL) in receiving vial 13 by argon flow. .. The crude [18 F]FPEB product mixture in receiving vial 13 was loaded into a 5 mL stainless steel loop and injected onto the semi-preparative HPLC COLUMN . .. The chromatogram was monitored with tandem radioactivity and UV (absorbance at 254 nm) detectors.

Purification:

Article Title: Application of Neurokinin-1 Receptor in Targeted Strategies for Glioma Treatment. Part I: Synthesis and Evaluation of Substance P Fragments Labeled with 99mTc and 177Lu as Potential Receptor Radiopharmaceuticals
Article Snippet: .. Crude CN-SP products ( ) were purified on a semi-preparative HPLC column (system 1; Phenomenex, Torrance, CA, USA) and lyophilized (yield ≈85–95%). .. MS of CN-SP(1–11): m/z : calc.

Flow Cytometry:

Article Title: Preparation of the Metabotropic Glutamate Receptor 5 (mGluR5) PET Tracer [18F]FPEB for Human Use: An Automated Radiosynthesis and a Novel One-Pot Synthesis of its Radiolabeling Precursor
Article Snippet: A 10 mL sterile empty vial ( DOSE VIAL ) attached with a Millipore Millex® GV membrane filter (0.22 µm, 33 mm) and a sterile venting needle was connected to the end of product line from valve v16 . .. A semi-preparative HPLC COLUMN was put in place and equilibrated with the mobile phase for 25 min at a flow rate of 5 mL/min before use. ..

Article Title: Synthesis, characterization, and preclinical validation of a PET radiopharmaceutical for interrogating Aβ (β-amyloid) plaques in Alzheimer’s disease
Article Snippet: .. The PET tracer 18 F- 7B eluted as a single chemical entity (radiochemical purity > 95%), with a retention time of 9.5 min on a semi-preparative HPLC column (Phenomenex Luna® C18; 100 Å) (5 μm, 250 × 10 mm; flow rate, 3 mL/min). .. The appropriate fraction eluting at 9.5 min was collected, concentrated, dissolved in 10% ethanol in saline, and employed for bioassays (specific activity: 1,200 to 1,400 Ci/mmol ).

Positron Emission Tomography:

Article Title: Synthesis, characterization, and preclinical validation of a PET radiopharmaceutical for interrogating Aβ (β-amyloid) plaques in Alzheimer’s disease
Article Snippet: .. The PET tracer 18 F- 7B eluted as a single chemical entity (radiochemical purity > 95%), with a retention time of 9.5 min on a semi-preparative HPLC column (Phenomenex Luna® C18; 100 Å) (5 μm, 250 × 10 mm; flow rate, 3 mL/min). .. The appropriate fraction eluting at 9.5 min was collected, concentrated, dissolved in 10% ethanol in saline, and employed for bioassays (specific activity: 1,200 to 1,400 Ci/mmol ).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Phenomenex semi preparative hplc system
    <t>HPLC</t> monitoring of coupling reaction between 18 F-sugar and 5-Iodo-uracil, heated using microwave for 15 min. Method 1 (details in method section), 0.05%TFA in <t>water/AcCN</t> as mobile phase. Red line: sample was from radiosynthesis; Black line: sample from
    Semi Preparative Hplc System, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative hplc system/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    semi preparative hplc system - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Phenomenex semi preparative hplc column
    <t>HPLC</t> chromatograms (system 1) of reaction mixtures of <t>DOTA-SP(4–11),</t> DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.
    Semi Preparative Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative hplc column/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    semi preparative hplc column - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Phenomenex prodigy c18 reverse phase column
    (A) Probe synthesis. Reagents and conditions: (i) Fmoc-Rink amide linker, HOBt, DIC, DMF; (ii) 20% Piperidine/DMF; (iii) Monomer ( 6 ), DIPEA, DMAP, DCM/DMF; (iv) 2% Hydrazine/DMF; (v) [(a) Fmoc-AA-OH, Oxyma, DIC, DMF, (b) 20% piperidine/DMF x 6]; (vi) Methyl red, Oxyma, DIC, DMF,; (vii) (a) 2% Hydrazine/DMF, b) 5(6)-carboxyfluorescein, Oxyma, DIC, DMF; (ix) TFA/DCM/TIS (95/2.5/2.5). MR = Methyl red, FAM = 5(6)-carboxyfluorescein amide, Ahx = 6-aminohexanoic acid, Dde: N-(1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl). (B) RP-HPLC chromatogram of probe ( 8 ) on a Discovery <t>C18</t> reverse-phase column (50 × 4.6 mm, 5 μm) with a flow rate of 1 mL/min and eluting with 0.1% HCOOH in H 2 O (A) and 0.1% HCOOH in CH 3 CN (B) , a gradient of 5 to 95% B over 13 min and an initial isocratic period of 2 min with detection at 254 nm (lower) and by evaporative light scattering (upper); (C) FTMS analysis: m/z 1030 [M + 4/4] + , 1373 [M + 3/3] + and 2059 [M + 2/2] + ; insert spectral zoom (experimental and theoretical [M + 3/3] + ).
    Prodigy C18 Reverse Phase Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prodigy c18 reverse phase column/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prodigy c18 reverse phase column - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Phenomenex semi preparative hplc
    Chromatogramm of the <t>HPLC-DAD</t> analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: <t>Phenomenex</t> Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.
    Semi Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative hplc/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    semi preparative hplc - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    HPLC monitoring of coupling reaction between 18 F-sugar and 5-Iodo-uracil, heated using microwave for 15 min. Method 1 (details in method section), 0.05%TFA in water/AcCN as mobile phase. Red line: sample was from radiosynthesis; Black line: sample from

    Journal: Nuclear medicine and biology

    Article Title: An improved strategy for the synthesis of [18F]-labeled arabinofuranosyl nuclosides

    doi: 10.1016/j.nucmedbio.2012.06.008

    Figure Lengend Snippet: HPLC monitoring of coupling reaction between 18 F-sugar and 5-Iodo-uracil, heated using microwave for 15 min. Method 1 (details in method section), 0.05%TFA in water/AcCN as mobile phase. Red line: sample was from radiosynthesis; Black line: sample from

    Article Snippet: The crude mixture containing the product ( 4) was dissolved in AcCN/H2 O solution (v/v=2/98) and loaded for purification with semi-preparative HPLC system ( Method 2 column: Phenomenex® Luna C18(2) (250 mm × 10.0 mm, 5 mm, 100 Å); UV detector: 280 nm; flow rate: 7 mL/min; mobile phase: 0.1% TFA in water and AcCN); gradient: 0–1 min, 2%–10% AcCN, 1–16 min, 10%–20% AcCN, 16–18.5 min, 20%–100% AcCN, 24.5min, 100% AcCN, 25min, 2% AcCN).

    Techniques: High Performance Liquid Chromatography

    HPLC chromatograms (system 1) of reaction mixtures of DOTA-SP(4–11), DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Application of Neurokinin-1 Receptor in Targeted Strategies for Glioma Treatment. Part I: Synthesis and Evaluation of Substance P Fragments Labeled with 99mTc and 177Lu as Potential Receptor Radiopharmaceuticals

    doi: 10.3390/molecules23102542

    Figure Lengend Snippet: HPLC chromatograms (system 1) of reaction mixtures of DOTA-SP(4–11), DOTA-SP(5–11) and DOTA-[Thi 8 ,Met(O 2 ) 11 ]SP(5–11) bioconjugates.

    Article Snippet: Crude DOTA-SP products ( ) were purified on a semi-preparative HPLC column (system 1) and lyophilized (yield ≈80–85%).

    Techniques: High Performance Liquid Chromatography

    (A) Probe synthesis. Reagents and conditions: (i) Fmoc-Rink amide linker, HOBt, DIC, DMF; (ii) 20% Piperidine/DMF; (iii) Monomer ( 6 ), DIPEA, DMAP, DCM/DMF; (iv) 2% Hydrazine/DMF; (v) [(a) Fmoc-AA-OH, Oxyma, DIC, DMF, (b) 20% piperidine/DMF x 6]; (vi) Methyl red, Oxyma, DIC, DMF,; (vii) (a) 2% Hydrazine/DMF, b) 5(6)-carboxyfluorescein, Oxyma, DIC, DMF; (ix) TFA/DCM/TIS (95/2.5/2.5). MR = Methyl red, FAM = 5(6)-carboxyfluorescein amide, Ahx = 6-aminohexanoic acid, Dde: N-(1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl). (B) RP-HPLC chromatogram of probe ( 8 ) on a Discovery C18 reverse-phase column (50 × 4.6 mm, 5 μm) with a flow rate of 1 mL/min and eluting with 0.1% HCOOH in H 2 O (A) and 0.1% HCOOH in CH 3 CN (B) , a gradient of 5 to 95% B over 13 min and an initial isocratic period of 2 min with detection at 254 nm (lower) and by evaporative light scattering (upper); (C) FTMS analysis: m/z 1030 [M + 4/4] + , 1373 [M + 3/3] + and 2059 [M + 2/2] + ; insert spectral zoom (experimental and theoretical [M + 3/3] + ).

    Journal: Scientific Reports

    Article Title: Super-silent FRET Sensor Enables Live Cell Imaging and Flow Cytometric Stratification of Intracellular Serine Protease Activity in Neutrophils

    doi: 10.1038/s41598-018-31391-9

    Figure Lengend Snippet: (A) Probe synthesis. Reagents and conditions: (i) Fmoc-Rink amide linker, HOBt, DIC, DMF; (ii) 20% Piperidine/DMF; (iii) Monomer ( 6 ), DIPEA, DMAP, DCM/DMF; (iv) 2% Hydrazine/DMF; (v) [(a) Fmoc-AA-OH, Oxyma, DIC, DMF, (b) 20% piperidine/DMF x 6]; (vi) Methyl red, Oxyma, DIC, DMF,; (vii) (a) 2% Hydrazine/DMF, b) 5(6)-carboxyfluorescein, Oxyma, DIC, DMF; (ix) TFA/DCM/TIS (95/2.5/2.5). MR = Methyl red, FAM = 5(6)-carboxyfluorescein amide, Ahx = 6-aminohexanoic acid, Dde: N-(1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl). (B) RP-HPLC chromatogram of probe ( 8 ) on a Discovery C18 reverse-phase column (50 × 4.6 mm, 5 μm) with a flow rate of 1 mL/min and eluting with 0.1% HCOOH in H 2 O (A) and 0.1% HCOOH in CH 3 CN (B) , a gradient of 5 to 95% B over 13 min and an initial isocratic period of 2 min with detection at 254 nm (lower) and by evaporative light scattering (upper); (C) FTMS analysis: m/z 1030 [M + 4/4] + , 1373 [M + 3/3] + and 2059 [M + 2/2] + ; insert spectral zoom (experimental and theoretical [M + 3/3] + ).

    Article Snippet: Semi-preparative RP–HPLC was performed on an Agilent 1100 system equipped with a Phenomenex Prodigy C18 reverse-phase column (250 × 10 mm, 5 μm) with a flow rate 2.5 mL/min and eluting with 0.1% HCOOH in H2 O (A) and 0.1% HCOOH in CH3 CN (B), with a gradient of 5 to 95% B over 25 min and an initial isocratic period of 2 min. Electrospray ionization mass spectrometry (ESI–MS) analyses were carried out on an Agilent Technologies LC/MSD Series 1100 quadrupole mass spectrometer (QMS) in an ESI mode.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry

    Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum

    doi: 10.3390/molecules22061003

    Figure Lengend Snippet: Chromatogramm of the HPLC-DAD analysis of the investigated dichloromethane (DCM) extract of the roots of Doronicum austriacum (2.5 mg/mL MeOH) at 205 nm. Analytical conditions: stationary phase: Phenomenex Synergi Max-RP 80 Å, 4 µm (4.6 mm × 150 mm); temperature: 35 °C; mobile phase: A = water + 0.025% TFA, B = acetonitrile; flow rate: 1.00 mL/min; detection: 205 nm; injection volume: 10 µL; solvent composition during analysis: 0′: 75% A; 10′: 60% A; 30′: 30% A; 35′: 2% A; 45′: stop; post time: 10′.

    Article Snippet: A fraction eluted with 300–980 mL (53.89 mg) was further purified by semi-preparative HPLC (stationary phase: Phenomenex Aqua C18 column (5 µm; 250 × 10 mm); mobile phase: solvent A: water solvent B: acetonitrile, gradient elution: 0 min: 45% A; 16 min: 2% A; 21 min: 45% A; 30 min: stop; flow 2.00 mL/min; 40 °C; 150 µL injection volume of a solution of 18.00 mg/mL methanol).

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection