selective reaction monitoring mode hplc electrospray ionization esi tandem mass spectrometry  (SCIEX)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85

    Structured Review

    SCIEX selective reaction monitoring mode hplc electrospray ionization esi tandem mass spectrometry
    Nuclear localization of lipin1 is promoted by pharmacological inhibition of phospholipase D. (A) HEK 293 cells expressing wild-type lipin1β and the 4A and 9A polybasic motif mutants were treated with 1.5 μM of the dual PLD1/PLD2 inhibitor FIPI or 50 μM of the PLD2 selective inhibitor CAY10593 for 4h. Subcellular localization of lipin1β (green) was visualized by indirect immunofluorescence and compared with the nuclear marker DAPI (blue). (B) Total PA levels in cells incubated with vehicle or the indicated PLD inhibitors at the concentrations used for the experiment shown in panel A and total PA levels (the sum of 16 abundant PA species) quantitated by <t>HPLC</t> <t>ESI</t> MS/MS. (C) Percent of total number of transfected cells, which lipin1β was localized in cytoplasm, nucleus, or both cytoplasm and nucleus (mean ± SD of at least 100 cells counted in 10 distinct fields).
    Selective Reaction Monitoring Mode Hplc Electrospray Ionization Esi Tandem Mass Spectrometry, supplied by SCIEX, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/selective reaction monitoring mode hplc electrospray ionization esi tandem mass spectrometry/product/SCIEX
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    selective reaction monitoring mode hplc electrospray ionization esi tandem mass spectrometry - by Bioz Stars, 2020-09
    85/100 stars

    Related Products / Commonly Used Together

    phosphatidylcholine pc

    Images

    1) Product Images from "A Phosphatidic Acid Binding/Nuclear Localization Motif Determines Lipin1 Function in Lipid Metabolism and Adipogenesis"

    Article Title: A Phosphatidic Acid Binding/Nuclear Localization Motif Determines Lipin1 Function in Lipid Metabolism and Adipogenesis

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E10-01-0073

    Nuclear localization of lipin1 is promoted by pharmacological inhibition of phospholipase D. (A) HEK 293 cells expressing wild-type lipin1β and the 4A and 9A polybasic motif mutants were treated with 1.5 μM of the dual PLD1/PLD2 inhibitor FIPI or 50 μM of the PLD2 selective inhibitor CAY10593 for 4h. Subcellular localization of lipin1β (green) was visualized by indirect immunofluorescence and compared with the nuclear marker DAPI (blue). (B) Total PA levels in cells incubated with vehicle or the indicated PLD inhibitors at the concentrations used for the experiment shown in panel A and total PA levels (the sum of 16 abundant PA species) quantitated by HPLC ESI MS/MS. (C) Percent of total number of transfected cells, which lipin1β was localized in cytoplasm, nucleus, or both cytoplasm and nucleus (mean ± SD of at least 100 cells counted in 10 distinct fields).
    Figure Legend Snippet: Nuclear localization of lipin1 is promoted by pharmacological inhibition of phospholipase D. (A) HEK 293 cells expressing wild-type lipin1β and the 4A and 9A polybasic motif mutants were treated with 1.5 μM of the dual PLD1/PLD2 inhibitor FIPI or 50 μM of the PLD2 selective inhibitor CAY10593 for 4h. Subcellular localization of lipin1β (green) was visualized by indirect immunofluorescence and compared with the nuclear marker DAPI (blue). (B) Total PA levels in cells incubated with vehicle or the indicated PLD inhibitors at the concentrations used for the experiment shown in panel A and total PA levels (the sum of 16 abundant PA species) quantitated by HPLC ESI MS/MS. (C) Percent of total number of transfected cells, which lipin1β was localized in cytoplasm, nucleus, or both cytoplasm and nucleus (mean ± SD of at least 100 cells counted in 10 distinct fields).

    Techniques Used: Inhibition, Expressing, Immunofluorescence, Marker, Incubation, High Performance Liquid Chromatography, Mass Spectrometry, Transfection

    The polybasic motif plays an important role in complementation of the adipogenesis defect of fld/fld mouse embryo fibroblasts by lipin1β. (A) Oil red-O staining (red) of differentiated wt and fld/fld mouse embryo fibroblasts expressing wild-type lipin1β, the indicated lipin1β mutants, or a GFP control using lentivirus vectors at day 6 postinduction of differentiation. (B) Expression of lipin 1 variants was quantitated by Western blotting. (C) Adipogenic differentiation was quantitated by counting the number of oil red-O–stained colonies per field. (D) Adipogenic differentiation was quantitated by measuring total TG levels (the sum of 36 abundant TG species) using HPLC ESI MS/MS. Data shown are means ± SD of three or more independent determinations. Statistically significant differences between cells expressing wt lipin 1 and the indicated lipin 1 mutants are indicated by asterisks. Note that TG accumulation or the number of differentiated colonies was not significantly different (N.S.) between cells expressing GFP or Lipin1 D712E.
    Figure Legend Snippet: The polybasic motif plays an important role in complementation of the adipogenesis defect of fld/fld mouse embryo fibroblasts by lipin1β. (A) Oil red-O staining (red) of differentiated wt and fld/fld mouse embryo fibroblasts expressing wild-type lipin1β, the indicated lipin1β mutants, or a GFP control using lentivirus vectors at day 6 postinduction of differentiation. (B) Expression of lipin 1 variants was quantitated by Western blotting. (C) Adipogenic differentiation was quantitated by counting the number of oil red-O–stained colonies per field. (D) Adipogenic differentiation was quantitated by measuring total TG levels (the sum of 36 abundant TG species) using HPLC ESI MS/MS. Data shown are means ± SD of three or more independent determinations. Statistically significant differences between cells expressing wt lipin 1 and the indicated lipin 1 mutants are indicated by asterisks. Note that TG accumulation or the number of differentiated colonies was not significantly different (N.S.) between cells expressing GFP or Lipin1 D712E.

    Techniques Used: Staining, Expressing, Western Blot, High Performance Liquid Chromatography, Mass Spectrometry

    Effects of overexpression of lipin1β wt and mutants on diacylglycerol (DG), phosphatidylcholine (PC), and phosphatidic acid (PA) levels. (A) HA-tagged wt lipin1β and the indicated mutants were overexpressed in HepG2 cells and detected by Western blotting. (B) HepG2 cells expressing wt lipin1β and the indicated mutants were radiolabeled with [ 3 H]palmitic acid for 24 h. DG levels were determined after separation by TLC, and quantitation by scintillation counting. (C–E) Levels of 16 abundant molecular species of PA, DG, and PC were determined in HepG2 cells overexpressing lipin1β wt, and mutants incubated with 1 μM palmitic acid were measured by HPLC ESI MS/MS. Data are means ± SD of at least three independent determinations.
    Figure Legend Snippet: Effects of overexpression of lipin1β wt and mutants on diacylglycerol (DG), phosphatidylcholine (PC), and phosphatidic acid (PA) levels. (A) HA-tagged wt lipin1β and the indicated mutants were overexpressed in HepG2 cells and detected by Western blotting. (B) HepG2 cells expressing wt lipin1β and the indicated mutants were radiolabeled with [ 3 H]palmitic acid for 24 h. DG levels were determined after separation by TLC, and quantitation by scintillation counting. (C–E) Levels of 16 abundant molecular species of PA, DG, and PC were determined in HepG2 cells overexpressing lipin1β wt, and mutants incubated with 1 μM palmitic acid were measured by HPLC ESI MS/MS. Data are means ± SD of at least three independent determinations.

    Techniques Used: Over Expression, Western Blot, Expressing, Thin Layer Chromatography, Quantitation Assay, Incubation, High Performance Liquid Chromatography, Mass Spectrometry

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: A Phosphatidic Acid Binding/Nuclear Localization Motif Determines Lipin1 Function in Lipid Metabolism and Adipogenesis
    Article Snippet: .. Molecular species of TG, DG, PA, and phosphatidylcholine (PC) were quantitated by selective reaction monitoring mode HPLC- electrospray ionization (ESI) tandem mass spectrometry using an AB Sciex (Foster City, CA) 4000 Q-Trap hybrid linear ion trap triple-quadrupole mass spectrometer equipped with a Turbo V electrospray ion source. ..

    Mass Spectrometry:

    Article Title: A Phosphatidic Acid Binding/Nuclear Localization Motif Determines Lipin1 Function in Lipid Metabolism and Adipogenesis
    Article Snippet: .. Molecular species of TG, DG, PA, and phosphatidylcholine (PC) were quantitated by selective reaction monitoring mode HPLC- electrospray ionization (ESI) tandem mass spectrometry using an AB Sciex (Foster City, CA) 4000 Q-Trap hybrid linear ion trap triple-quadrupole mass spectrometer equipped with a Turbo V electrospray ion source. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    SCIEX selective reaction monitoring mode hplc electrospray ionization esi tandem mass spectrometry
    Nuclear localization of lipin1 is promoted by pharmacological inhibition of phospholipase D. (A) HEK 293 cells expressing wild-type lipin1β and the 4A and 9A polybasic motif mutants were treated with 1.5 μM of the dual PLD1/PLD2 inhibitor FIPI or 50 μM of the PLD2 selective inhibitor CAY10593 for 4h. Subcellular localization of lipin1β (green) was visualized by indirect immunofluorescence and compared with the nuclear marker DAPI (blue). (B) Total PA levels in cells incubated with vehicle or the indicated PLD inhibitors at the concentrations used for the experiment shown in panel A and total PA levels (the sum of 16 abundant PA species) quantitated by <t>HPLC</t> <t>ESI</t> MS/MS. (C) Percent of total number of transfected cells, which lipin1β was localized in cytoplasm, nucleus, or both cytoplasm and nucleus (mean ± SD of at least 100 cells counted in 10 distinct fields).
    Selective Reaction Monitoring Mode Hplc Electrospray Ionization Esi Tandem Mass Spectrometry, supplied by SCIEX, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/selective reaction monitoring mode hplc electrospray ionization esi tandem mass spectrometry/product/SCIEX
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    selective reaction monitoring mode hplc electrospray ionization esi tandem mass spectrometry - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Nuclear localization of lipin1 is promoted by pharmacological inhibition of phospholipase D. (A) HEK 293 cells expressing wild-type lipin1β and the 4A and 9A polybasic motif mutants were treated with 1.5 μM of the dual PLD1/PLD2 inhibitor FIPI or 50 μM of the PLD2 selective inhibitor CAY10593 for 4h. Subcellular localization of lipin1β (green) was visualized by indirect immunofluorescence and compared with the nuclear marker DAPI (blue). (B) Total PA levels in cells incubated with vehicle or the indicated PLD inhibitors at the concentrations used for the experiment shown in panel A and total PA levels (the sum of 16 abundant PA species) quantitated by HPLC ESI MS/MS. (C) Percent of total number of transfected cells, which lipin1β was localized in cytoplasm, nucleus, or both cytoplasm and nucleus (mean ± SD of at least 100 cells counted in 10 distinct fields).

    Journal: Molecular Biology of the Cell

    Article Title: A Phosphatidic Acid Binding/Nuclear Localization Motif Determines Lipin1 Function in Lipid Metabolism and Adipogenesis

    doi: 10.1091/mbc.E10-01-0073

    Figure Lengend Snippet: Nuclear localization of lipin1 is promoted by pharmacological inhibition of phospholipase D. (A) HEK 293 cells expressing wild-type lipin1β and the 4A and 9A polybasic motif mutants were treated with 1.5 μM of the dual PLD1/PLD2 inhibitor FIPI or 50 μM of the PLD2 selective inhibitor CAY10593 for 4h. Subcellular localization of lipin1β (green) was visualized by indirect immunofluorescence and compared with the nuclear marker DAPI (blue). (B) Total PA levels in cells incubated with vehicle or the indicated PLD inhibitors at the concentrations used for the experiment shown in panel A and total PA levels (the sum of 16 abundant PA species) quantitated by HPLC ESI MS/MS. (C) Percent of total number of transfected cells, which lipin1β was localized in cytoplasm, nucleus, or both cytoplasm and nucleus (mean ± SD of at least 100 cells counted in 10 distinct fields).

    Article Snippet: Molecular species of TG, DG, PA, and phosphatidylcholine (PC) were quantitated by selective reaction monitoring mode HPLC- electrospray ionization (ESI) tandem mass spectrometry using an AB Sciex (Foster City, CA) 4000 Q-Trap hybrid linear ion trap triple-quadrupole mass spectrometer equipped with a Turbo V electrospray ion source.

    Techniques: Inhibition, Expressing, Immunofluorescence, Marker, Incubation, High Performance Liquid Chromatography, Mass Spectrometry, Transfection

    The polybasic motif plays an important role in complementation of the adipogenesis defect of fld/fld mouse embryo fibroblasts by lipin1β. (A) Oil red-O staining (red) of differentiated wt and fld/fld mouse embryo fibroblasts expressing wild-type lipin1β, the indicated lipin1β mutants, or a GFP control using lentivirus vectors at day 6 postinduction of differentiation. (B) Expression of lipin 1 variants was quantitated by Western blotting. (C) Adipogenic differentiation was quantitated by counting the number of oil red-O–stained colonies per field. (D) Adipogenic differentiation was quantitated by measuring total TG levels (the sum of 36 abundant TG species) using HPLC ESI MS/MS. Data shown are means ± SD of three or more independent determinations. Statistically significant differences between cells expressing wt lipin 1 and the indicated lipin 1 mutants are indicated by asterisks. Note that TG accumulation or the number of differentiated colonies was not significantly different (N.S.) between cells expressing GFP or Lipin1 D712E.

    Journal: Molecular Biology of the Cell

    Article Title: A Phosphatidic Acid Binding/Nuclear Localization Motif Determines Lipin1 Function in Lipid Metabolism and Adipogenesis

    doi: 10.1091/mbc.E10-01-0073

    Figure Lengend Snippet: The polybasic motif plays an important role in complementation of the adipogenesis defect of fld/fld mouse embryo fibroblasts by lipin1β. (A) Oil red-O staining (red) of differentiated wt and fld/fld mouse embryo fibroblasts expressing wild-type lipin1β, the indicated lipin1β mutants, or a GFP control using lentivirus vectors at day 6 postinduction of differentiation. (B) Expression of lipin 1 variants was quantitated by Western blotting. (C) Adipogenic differentiation was quantitated by counting the number of oil red-O–stained colonies per field. (D) Adipogenic differentiation was quantitated by measuring total TG levels (the sum of 36 abundant TG species) using HPLC ESI MS/MS. Data shown are means ± SD of three or more independent determinations. Statistically significant differences between cells expressing wt lipin 1 and the indicated lipin 1 mutants are indicated by asterisks. Note that TG accumulation or the number of differentiated colonies was not significantly different (N.S.) between cells expressing GFP or Lipin1 D712E.

    Article Snippet: Molecular species of TG, DG, PA, and phosphatidylcholine (PC) were quantitated by selective reaction monitoring mode HPLC- electrospray ionization (ESI) tandem mass spectrometry using an AB Sciex (Foster City, CA) 4000 Q-Trap hybrid linear ion trap triple-quadrupole mass spectrometer equipped with a Turbo V electrospray ion source.

    Techniques: Staining, Expressing, Western Blot, High Performance Liquid Chromatography, Mass Spectrometry

    Effects of overexpression of lipin1β wt and mutants on diacylglycerol (DG), phosphatidylcholine (PC), and phosphatidic acid (PA) levels. (A) HA-tagged wt lipin1β and the indicated mutants were overexpressed in HepG2 cells and detected by Western blotting. (B) HepG2 cells expressing wt lipin1β and the indicated mutants were radiolabeled with [ 3 H]palmitic acid for 24 h. DG levels were determined after separation by TLC, and quantitation by scintillation counting. (C–E) Levels of 16 abundant molecular species of PA, DG, and PC were determined in HepG2 cells overexpressing lipin1β wt, and mutants incubated with 1 μM palmitic acid were measured by HPLC ESI MS/MS. Data are means ± SD of at least three independent determinations.

    Journal: Molecular Biology of the Cell

    Article Title: A Phosphatidic Acid Binding/Nuclear Localization Motif Determines Lipin1 Function in Lipid Metabolism and Adipogenesis

    doi: 10.1091/mbc.E10-01-0073

    Figure Lengend Snippet: Effects of overexpression of lipin1β wt and mutants on diacylglycerol (DG), phosphatidylcholine (PC), and phosphatidic acid (PA) levels. (A) HA-tagged wt lipin1β and the indicated mutants were overexpressed in HepG2 cells and detected by Western blotting. (B) HepG2 cells expressing wt lipin1β and the indicated mutants were radiolabeled with [ 3 H]palmitic acid for 24 h. DG levels were determined after separation by TLC, and quantitation by scintillation counting. (C–E) Levels of 16 abundant molecular species of PA, DG, and PC were determined in HepG2 cells overexpressing lipin1β wt, and mutants incubated with 1 μM palmitic acid were measured by HPLC ESI MS/MS. Data are means ± SD of at least three independent determinations.

    Article Snippet: Molecular species of TG, DG, PA, and phosphatidylcholine (PC) were quantitated by selective reaction monitoring mode HPLC- electrospray ionization (ESI) tandem mass spectrometry using an AB Sciex (Foster City, CA) 4000 Q-Trap hybrid linear ion trap triple-quadrupole mass spectrometer equipped with a Turbo V electrospray ion source.

    Techniques: Over Expression, Western Blot, Expressing, Thin Layer Chromatography, Quantitation Assay, Incubation, High Performance Liquid Chromatography, Mass Spectrometry