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Figure 6. <t>Eprinomectin</t> inhibits B19V replication more selectively than IVM. UT7/EpoS1 were either treated with increasing concentrations of indicated compounds for 48 h and then cell viability measured by using the alamarBlue™HS Cell Viability Reagent (A,C,E) or treated for 6 h with increasing concentrations of the indicated compounds then infected for 2 h with and incubated for further 48 hpi to allow quantification of viral DNA replication as described (B,D,F). Data were normalized to untreated cells and shown as mean ± SD relative to three independent experiments. Half maximal cytotoxicity concentration (CC50) and half maximal effective concentration (EC50) were calculated as described in the Section 2.
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Figure 6. Eprinomectin inhibits B19V replication more selectively than IVM. UT7/EpoS1 were either treated with increasing concentrations of indicated compounds for 48 h and then cell viability measured by using the alamarBlue™HS Cell Viability Reagent (A,C,E) or treated for 6 h with increasing concentrations of the indicated compounds then infected for 2 h with and incubated for further 48 hpi to allow quantification of viral DNA replication as described (B,D,F). Data were normalized to untreated cells and shown as mean ± SD relative to three independent experiments. Half maximal cytotoxicity concentration (CC50) and half maximal effective concentration (EC50) were calculated as described in the Section 2.

Journal: Viruses

Article Title: Avermectins Inhibit Replication of Parvovirus B19 by Disrupting the Interaction Between Importin α and Non-Structural Protein 1.

doi: 10.3390/v17020220

Figure Lengend Snippet: Figure 6. Eprinomectin inhibits B19V replication more selectively than IVM. UT7/EpoS1 were either treated with increasing concentrations of indicated compounds for 48 h and then cell viability measured by using the alamarBlue™HS Cell Viability Reagent (A,C,E) or treated for 6 h with increasing concentrations of the indicated compounds then infected for 2 h with and incubated for further 48 hpi to allow quantification of viral DNA replication as described (B,D,F). Data were normalized to untreated cells and shown as mean ± SD relative to three independent experiments. Half maximal cytotoxicity concentration (CC50) and half maximal effective concentration (EC50) were calculated as described in the Section 2.

Article Snippet: Importazole (#S8446), Avermectin B1 (#S4999), Eprinomectin (#S3591), Selamectin (#S5247), Emamectin benzoate (#S4423), Milbemycin oxime (#S5055), Moxidectin (#S3713), and Ivermectin (#S1351) were purchased from Selleckchem (Houston, TX, USA).

Techniques: Infection, Incubation, Concentration Assay

Figure 7. IVM and Eprinomectin exhibit high cytotoxicity in EPCs. EPCs were either treated with increasing concentrations of indicated compounds for 48 h to allow measurement of cell viability using a WST-8 based assay (A,C) or treated for 6 h with increasing concentrations of the indicated compounds then infected for 2 h and further incubated for 48 hpi to allow quantification of vi- ral DNA replication as described (B,D). Data were normalized to untreated cells and shown as mean ± SD relative to three independent experiments. Half maximal cytotoxicity concentration (CC50) and half maximal effective concentration (EC50) were calculated as described in the Section 2. TLTM: too low to measure.

Journal: Viruses

Article Title: Avermectins Inhibit Replication of Parvovirus B19 by Disrupting the Interaction Between Importin α and Non-Structural Protein 1.

doi: 10.3390/v17020220

Figure Lengend Snippet: Figure 7. IVM and Eprinomectin exhibit high cytotoxicity in EPCs. EPCs were either treated with increasing concentrations of indicated compounds for 48 h to allow measurement of cell viability using a WST-8 based assay (A,C) or treated for 6 h with increasing concentrations of the indicated compounds then infected for 2 h and further incubated for 48 hpi to allow quantification of vi- ral DNA replication as described (B,D). Data were normalized to untreated cells and shown as mean ± SD relative to three independent experiments. Half maximal cytotoxicity concentration (CC50) and half maximal effective concentration (EC50) were calculated as described in the Section 2. TLTM: too low to measure.

Article Snippet: Importazole (#S8446), Avermectin B1 (#S4999), Eprinomectin (#S3591), Selamectin (#S5247), Emamectin benzoate (#S4423), Milbemycin oxime (#S5055), Moxidectin (#S3713), and Ivermectin (#S1351) were purchased from Selleckchem (Houston, TX, USA).

Techniques: Infection, Incubation, Concentration Assay