secondary peroxidase conjugated antibodies  (Abcam)

 
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    Structured Review

    Abcam secondary peroxidase conjugated antibodies
    Secondary Peroxidase Conjugated Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary peroxidase conjugated antibodies/product/Abcam
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary peroxidase conjugated antibodies - by Bioz Stars, 2020-07
    90/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Ultrasound-Mediated Mesenchymal Stem Cells Transfection as a Targeted Cancer Therapy Platform
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) Three days following TUS application, conditioned media of the transfected cells was collected and PEX was detected in the conditioned media using ELISA with primary antibodies against MMP2 and secondary peroxidase-conjugated antibodies (Abcam, Cambridge, MA). ..

    Transfection:

    Article Title: Ultrasound-Mediated Mesenchymal Stem Cells Transfection as a Targeted Cancer Therapy Platform
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) Three days following TUS application, conditioned media of the transfected cells was collected and PEX was detected in the conditioned media using ELISA with primary antibodies against MMP2 and secondary peroxidase-conjugated antibodies (Abcam, Cambridge, MA). ..

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    Abcam peroxidase conjugated anti mouse secondary antibody
    Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a <t>mouse</t> monoclonal <t>anti-NIS</t> antibody and an anti-mouse <t>fluorescein-conjugated</t> <t>secondary</t> antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.
    Peroxidase Conjugated Anti Mouse Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated anti mouse secondary antibody/product/Abcam
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated anti mouse secondary antibody - by Bioz Stars, 2020-07
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    93
    Abcam hrp conjugated goat anti rabbit igg
    Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all <t>IgG</t> (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - <t>HRP</t> for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p
    Hrp Conjugated Goat Anti Rabbit Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam horse radish peroxidase hrp conjugated rabbit anti mouse secondary antibody
    Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse <t>anti-M13</t> phage antibody and <t>HRP-conjugated</t> rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells
    Horse Radish Peroxidase Hrp Conjugated Rabbit Anti Mouse Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse radish peroxidase hrp conjugated rabbit anti mouse secondary antibody/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    horse radish peroxidase hrp conjugated rabbit anti mouse secondary antibody - by Bioz Stars, 2020-07
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    92
    Abcam peroxidase conjugated secondary antibodies against nrf2
    Effect of <t>Nrf2</t> knockdown on Nrf2 and HO-1 in B16-F10 melanoma cells that were induced by ionizing radiation. B16-F10 melanoma cells were radiated by a dose of 8 Gy for 12 h following the treatment of melanoma cells with Nrf2 siRNA. The cells were harvested and western blotting was performed to detect the expression of (A) Nrf2 protein. (B) Quantitative analysis of Nrf2 protein expression. (C) Western blot analysis of HO-1 protein expression. (D) Quantitative analysis of HO-1 protein expression. (E) Reverse transcription-quantitative polymerase chain reaction of Nrf2 mRNA. (F) Analysis of HO-1 activity. All data are expressed as the mean ± standard deviation *P
    Peroxidase Conjugated Secondary Antibodies Against Nrf2, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.

    Journal: PLoS ONE

    Article Title: Resveratrol Inhibits Sodium/Iodide Symporter Gene Expression and Function in Rat Thyroid Cells

    doi: 10.1371/journal.pone.0107936

    Figure Lengend Snippet: Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.

    Article Snippet: The membranes were subsequently washed and incubated with a horseradish peroxidase-conjugated anti-mouse secondary antibody (ab6789, Abcam, Cambridge, UK), following the manufacturer instructions.

    Techniques: Expressing, Immunofluorescence, Staining, Negative Control, Microscopy

    Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all IgG (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - HRP for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p

    Journal: Inhalation toxicology

    Article Title: Mesothelial Cell Autoantibodies Upregulate Transcription Factors Associated with Fibrosis

    doi: 10.1080/08958378.2016.1271841

    Figure Lengend Snippet: Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all IgG (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - HRP for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p

    Article Snippet: The secondary antibody used was HRP-conjugated goat anti-rabbit IgG (ABCAM) diluted 1:1000 with 3%BSA/PBS.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Staining

    Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Identification of a Novel Tumor-Binding Peptide for Lung Cancer Through in-vitro Panning

    doi:

    Figure Lengend Snippet: Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Article Snippet: Mouse anti-M13 phage antibody and horse radish peroxidase (HRP)-conjugated rabbit anti-mouse secondary antibody were purchased from Abcam Inc (MA, USA).

    Techniques: Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Blocking Assay

    Effect of Nrf2 knockdown on Nrf2 and HO-1 in B16-F10 melanoma cells that were induced by ionizing radiation. B16-F10 melanoma cells were radiated by a dose of 8 Gy for 12 h following the treatment of melanoma cells with Nrf2 siRNA. The cells were harvested and western blotting was performed to detect the expression of (A) Nrf2 protein. (B) Quantitative analysis of Nrf2 protein expression. (C) Western blot analysis of HO-1 protein expression. (D) Quantitative analysis of HO-1 protein expression. (E) Reverse transcription-quantitative polymerase chain reaction of Nrf2 mRNA. (F) Analysis of HO-1 activity. All data are expressed as the mean ± standard deviation *P

    Journal: Oncology Letters

    Article Title: Migration and invasion in B16-F10 mouse melanoma cells are regulated by Nrf2 inhibition during treatment with ionizing radiation

    doi: 10.3892/ol.2018.8799

    Figure Lengend Snippet: Effect of Nrf2 knockdown on Nrf2 and HO-1 in B16-F10 melanoma cells that were induced by ionizing radiation. B16-F10 melanoma cells were radiated by a dose of 8 Gy for 12 h following the treatment of melanoma cells with Nrf2 siRNA. The cells were harvested and western blotting was performed to detect the expression of (A) Nrf2 protein. (B) Quantitative analysis of Nrf2 protein expression. (C) Western blot analysis of HO-1 protein expression. (D) Quantitative analysis of HO-1 protein expression. (E) Reverse transcription-quantitative polymerase chain reaction of Nrf2 mRNA. (F) Analysis of HO-1 activity. All data are expressed as the mean ± standard deviation *P

    Article Snippet: The membranes were incubated with horseradish peroxidase-conjugated secondary antibodies against Nrf2, HO-1, cleaved caspase-3 and β-actin (anti-mouse IgG; dilution, 1:10,000; cat. no. ab97046; anti-rabbit IgG; dilution, 1:10,000; cat. no. ab7090; Abcam) at room temperature for 1 h, which were detected using an enhanced chemiluminescence detection system (Amersham; GE Healthcare, Chicago, IL, USA).

    Techniques: Western Blot, Expressing, Real-time Polymerase Chain Reaction, Activity Assay, Standard Deviation

    Effect of Nrf2 knockdown on the viability, caspase-3 expression, invasion and migration of B16-F10 melanoma cells that were induced by ionizing radiation. B16-F10 melanoma cells were radiated by a dose of 8 Gy for 12 h following the treatment of melanoma cells with Nrf2 siRNA. The cells were harvested. Subsequently, (A) cell viability, (B) cleaved caspase-3 expression, (C) invasive and (D) migratory capacities were assessed. All data are expressed as the mean ± standard deviation *P

    Journal: Oncology Letters

    Article Title: Migration and invasion in B16-F10 mouse melanoma cells are regulated by Nrf2 inhibition during treatment with ionizing radiation

    doi: 10.3892/ol.2018.8799

    Figure Lengend Snippet: Effect of Nrf2 knockdown on the viability, caspase-3 expression, invasion and migration of B16-F10 melanoma cells that were induced by ionizing radiation. B16-F10 melanoma cells were radiated by a dose of 8 Gy for 12 h following the treatment of melanoma cells with Nrf2 siRNA. The cells were harvested. Subsequently, (A) cell viability, (B) cleaved caspase-3 expression, (C) invasive and (D) migratory capacities were assessed. All data are expressed as the mean ± standard deviation *P

    Article Snippet: The membranes were incubated with horseradish peroxidase-conjugated secondary antibodies against Nrf2, HO-1, cleaved caspase-3 and β-actin (anti-mouse IgG; dilution, 1:10,000; cat. no. ab97046; anti-rabbit IgG; dilution, 1:10,000; cat. no. ab7090; Abcam) at room temperature for 1 h, which were detected using an enhanced chemiluminescence detection system (Amersham; GE Healthcare, Chicago, IL, USA).

    Techniques: Expressing, Migration, Standard Deviation

    Effect of ionizing radiation on Nrf2 expression in B16-F10 melanoma cells. (A) B16-F10 melanoma cells were treated with various doses of ionizing radiation at 12 h. A single dose of 8 Gy was used to radiate B16-F10 melanoma at different time points. The cells were harvested to investigate the expression of (B) Nrf2 protein and (C) mRNA. All data are expressed as the mean ± standard deviation. *P

    Journal: Oncology Letters

    Article Title: Migration and invasion in B16-F10 mouse melanoma cells are regulated by Nrf2 inhibition during treatment with ionizing radiation

    doi: 10.3892/ol.2018.8799

    Figure Lengend Snippet: Effect of ionizing radiation on Nrf2 expression in B16-F10 melanoma cells. (A) B16-F10 melanoma cells were treated with various doses of ionizing radiation at 12 h. A single dose of 8 Gy was used to radiate B16-F10 melanoma at different time points. The cells were harvested to investigate the expression of (B) Nrf2 protein and (C) mRNA. All data are expressed as the mean ± standard deviation. *P

    Article Snippet: The membranes were incubated with horseradish peroxidase-conjugated secondary antibodies against Nrf2, HO-1, cleaved caspase-3 and β-actin (anti-mouse IgG; dilution, 1:10,000; cat. no. ab97046; anti-rabbit IgG; dilution, 1:10,000; cat. no. ab7090; Abcam) at room temperature for 1 h, which were detected using an enhanced chemiluminescence detection system (Amersham; GE Healthcare, Chicago, IL, USA).

    Techniques: Expressing, Standard Deviation