Structured Review

Promega secondary hrp conjugated antibodies
Secondary Hrp Conjugated Antibodies, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary hrp conjugated antibodies/product/Promega
Average 99 stars, based on 4 article reviews
Price from $9.99 to $1999.99
secondary hrp conjugated antibodies - by Bioz Stars, 2020-07
99/100 stars

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Mutagenesis:

Article Title: The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
Article Snippet: .. Immunoblotting of Yap5-myc wild type and mutant proteins were performed using 1:10000 mouse IgG Anti-cMyc (Roche) and 1:10000 anti-mouse IgG-HRP (Promega) as primary and secondary antibodies. .. Detection of the signals was performed using G:BOX Chemi XT4 (Syngene) following incubation with UptiLightTM HRP blot chemiluminescent ECL substrate (Interchim).

Incubation:

Article Title: Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins
Article Snippet: .. After incubation for 1 h, the membrane was washed with PBST (PBS containing 0.1% Tween 20) three times, and anti-mouse IgG HRP conjugate (Promega, Madison, WI) was poured on prior to incubation for 1 h. After washing with PBST three times, the bands were developed with ECL Plus reagents (GE Healthcare) and detected with a VersaDoc Imaging System (Bio-Rad). .. Indirect ELISA An indirect ELISA was performed to investigate the antigenicity of recombinant proteins and diagnose neosporosis.

Article Title: Elicitation of Both Anti HIV-1 Env Humoral and Cellular Immunities by Replicating Vaccinia Prime Sendai Virus Boost Regimen and Boosting by CD40Lm
Article Snippet: .. Plates were washed five times with PBS-T. Aliquots (100 µl) of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Promega, Sunnyvale, US), diluted 1∶2500 from the stock solution, were added and incubated for 1 h at room temperature. .. The plates were washed five times with PBS-T and TMB ELISA substrate solution (eBioscience, San Diego, US) was added.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: .. After washing six times with TBST, the plates were incubated with goat anti-mouse IgG conjugated with HRP (GAM-IgG-HRP, Promega, Madison, WI, 200 ng/mL in BB) for 1 hour at RT. .. Finally, SuperSignal West Pico Chemiluminescent Substrate (Pierce) was added and luminescence counts were measured in counts per second (cps) using the Victor-3 plate reader (Perkin-Elmer, Shelton, CT).

other:

Article Title: The Surface Layer of Tannerella forsythia Contributes to Serum Resistance and Oral Bacterial Coaggregation
Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-mouse IgG(H+L) (Promega, Madison, WI) and Alexa Fluor 594-conjugated goat anti-mouse IgG(H+L) (Molecular Probes Inc., Eugene, OR) were used for detection.

SDS Page:

Article Title: Inactivation of specific ? cell transcription factors in type 2 diabetes
Article Snippet: .. Proteins (10 μg loaded) were separated by 10% SDS-PAGE and tested by immunoblotting with the following antibodies: rabbit α-MAFA (Bethyl Laboratories, 1225, 1:2,000); mouse α-NKX6.1 (Developmental Studies Hybridoma Bank, 1:2,000); mouse α-ISL1 (Developmental Studies Hybridoma Bank, 1:1,000); goat α-HNF1α (Santa Cruz Biotechnology, 1:1,000); goat α-FOXA2 (Santa Cruz Biotechnology, 1:1,000); goat α-B56α (Santa Cruz Biotechnology, 1:1,000); goat α-LDB1 (CLIM-2) (Santa Cruz Biotechnology, 1:1,000); rabbit α-NEUROD1 (Epitomics, 1:1,000); mouse α-p/CAF (Santa Cruz Biotechnology, 1:1,000); mouse α-RBBP5 (RBQ3) (Santa Cruz Biotechnology, 1:1,000); mouse α-HB9 (Developmental Studies Hybridoma Bank, 1:1,000); mouse α-NKX2.2 (Developmental Studies Hybridoma Bank, 1:1,000); rabbit α-PDX1 (provided by Chris Wright, Vanderbilt University, Nashville, Tennessee, USA; 1:10,000); rabbit α-PAX6 (Covance, 1:1,000); mouse α-B55α (Santa Cruz Biotechnology, 1:1,000); rabbit α-FOXO1 (Cell Signaling Technology, 1:1,000); HRP-conjugated α-rabbit IgG (Promega, 1:2,000); and HRP-conjugated α-mouse IgG (Promega, 1:2,000). .. The experiments were performed at least 3 times, with representative results quantitated using NIH ImageJ software.

Imaging:

Article Title: Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins
Article Snippet: .. After incubation for 1 h, the membrane was washed with PBST (PBS containing 0.1% Tween 20) three times, and anti-mouse IgG HRP conjugate (Promega, Madison, WI) was poured on prior to incubation for 1 h. After washing with PBST three times, the bands were developed with ECL Plus reagents (GE Healthcare) and detected with a VersaDoc Imaging System (Bio-Rad). .. Indirect ELISA An indirect ELISA was performed to investigate the antigenicity of recombinant proteins and diagnose neosporosis.

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  • 88
    Promega horseradish peroxidise conjugated secondary antibodies
    Characterisation and purification of the royal jelly (RJ) component responsible for elevated MMP-9 production. ( A ) Heat and proteinase K treatment was performed by incubation of water royal jelly extract (WRJE) at 100 °C for 5 min and incubation with 150 μg/ml proteinase K for 1 h at 40 °C followed by heating to 98 °C for 10 min to inactivate the enzyme. Treated WRJE was incubated with HaCaT cells and conditioned equal volumes of the culture media were collected and subjected to gelatine zymography. Densitometric quantification of MMP-9 activity in culture media is presented. ( B ) Heat-treated WRJE was fractionated by a reverse phase-high performance liquid chromatography (RP-HPLC) on a C18 column (250 × 4.6 mm, 5 μm) at a flow rate 0.3 ml/min, with elution using a 10–90% gradient of acetonitrile (containing 0.1% (v/v) trifluoroacetic acid) for 85 min. ( C ) The HPLC fractions were assayed for MMP-9 induction. ( D ) HPLC fractions with maximal MMP-9 activity (51 to 59 min) were used for identification of MMP-9 inducer and were subjected to 16.5% Tricine-SDS-PAGE gels. Defensin-1 (5.5 kDa) was detected by Western blotting using a rabbit polyclonal anti-honeybee defensin-1 antibody diluted 1:2000 in blocking buffer. Horseradish <t>peroxidise-conjugated</t> secondary antibodies were applied. (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S3 ). White line in gel indicates the place where two gels were spliced together. Data are expressed as means and SEMs of three independent measurements. Asterisks indicate a significant difference from the untreated group, * P
    Horseradish Peroxidise Conjugated Secondary Antibodies, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidise conjugated secondary antibodies/product/Promega
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidise conjugated secondary antibodies - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    95
    Promega anti igg
    Humoral immune response characterization. Absolute anti-αSyn <t>IgM</t> (A) and relative anti-αSyn IgM (B) antibodies in serum from immunized mice. Absolute anti-αSyn <t>IgG</t> (C) and relative anti-αSyn IgG (D) antibodies in serum. The relative content of specific anti α-Syn IgM and IgG antibodies were calculated by dividing anti α-Syn IgM or IgG levels by the total corresponding antibody levels. AU: arbitrary units. Represented values are mean ± S.E.M. ( n = 5–7). Asterisks correspond to statistically significant differences between one particular group and the “vehicle” group. Hash signs indicate statistically significant differences between different groups. * /# P
    Anti Igg, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti igg/product/Promega
    Average 95 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    anti igg - by Bioz Stars, 2020-07
    95/100 stars
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    85
    Promega secondary iggs horseradish peroxidase conjugated hrp conjugated anti rabbit
    Humoral immune response characterization. Absolute anti-αSyn <t>IgM</t> (A) and relative anti-αSyn IgM (B) antibodies in serum from immunized mice. Absolute anti-αSyn <t>IgG</t> (C) and relative anti-αSyn IgG (D) antibodies in serum. The relative content of specific anti α-Syn IgM and IgG antibodies were calculated by dividing anti α-Syn IgM or IgG levels by the total corresponding antibody levels. AU: arbitrary units. Represented values are mean ± S.E.M. ( n = 5–7). Asterisks correspond to statistically significant differences between one particular group and the “vehicle” group. Hash signs indicate statistically significant differences between different groups. * /# P
    Secondary Iggs Horseradish Peroxidase Conjugated Hrp Conjugated Anti Rabbit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary iggs horseradish peroxidase conjugated hrp conjugated anti rabbit/product/Promega
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    secondary iggs horseradish peroxidase conjugated hrp conjugated anti rabbit - by Bioz Stars, 2020-07
    85/100 stars
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    92
    Promega secondary hrp conjugated antibodies
    Humoral immune response characterization. Absolute anti-αSyn <t>IgM</t> (A) and relative anti-αSyn IgM (B) antibodies in serum from immunized mice. Absolute anti-αSyn <t>IgG</t> (C) and relative anti-αSyn IgG (D) antibodies in serum. The relative content of specific anti α-Syn IgM and IgG antibodies were calculated by dividing anti α-Syn IgM or IgG levels by the total corresponding antibody levels. AU: arbitrary units. Represented values are mean ± S.E.M. ( n = 5–7). Asterisks correspond to statistically significant differences between one particular group and the “vehicle” group. Hash signs indicate statistically significant differences between different groups. * /# P
    Secondary Hrp Conjugated Antibodies, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary hrp conjugated antibodies/product/Promega
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    secondary hrp conjugated antibodies - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Characterisation and purification of the royal jelly (RJ) component responsible for elevated MMP-9 production. ( A ) Heat and proteinase K treatment was performed by incubation of water royal jelly extract (WRJE) at 100 °C for 5 min and incubation with 150 μg/ml proteinase K for 1 h at 40 °C followed by heating to 98 °C for 10 min to inactivate the enzyme. Treated WRJE was incubated with HaCaT cells and conditioned equal volumes of the culture media were collected and subjected to gelatine zymography. Densitometric quantification of MMP-9 activity in culture media is presented. ( B ) Heat-treated WRJE was fractionated by a reverse phase-high performance liquid chromatography (RP-HPLC) on a C18 column (250 × 4.6 mm, 5 μm) at a flow rate 0.3 ml/min, with elution using a 10–90% gradient of acetonitrile (containing 0.1% (v/v) trifluoroacetic acid) for 85 min. ( C ) The HPLC fractions were assayed for MMP-9 induction. ( D ) HPLC fractions with maximal MMP-9 activity (51 to 59 min) were used for identification of MMP-9 inducer and were subjected to 16.5% Tricine-SDS-PAGE gels. Defensin-1 (5.5 kDa) was detected by Western blotting using a rabbit polyclonal anti-honeybee defensin-1 antibody diluted 1:2000 in blocking buffer. Horseradish peroxidise-conjugated secondary antibodies were applied. (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S3 ). White line in gel indicates the place where two gels were spliced together. Data are expressed as means and SEMs of three independent measurements. Asterisks indicate a significant difference from the untreated group, * P

    Journal: Scientific Reports

    Article Title: Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

    doi: 10.1038/s41598-017-07494-0

    Figure Lengend Snippet: Characterisation and purification of the royal jelly (RJ) component responsible for elevated MMP-9 production. ( A ) Heat and proteinase K treatment was performed by incubation of water royal jelly extract (WRJE) at 100 °C for 5 min and incubation with 150 μg/ml proteinase K for 1 h at 40 °C followed by heating to 98 °C for 10 min to inactivate the enzyme. Treated WRJE was incubated with HaCaT cells and conditioned equal volumes of the culture media were collected and subjected to gelatine zymography. Densitometric quantification of MMP-9 activity in culture media is presented. ( B ) Heat-treated WRJE was fractionated by a reverse phase-high performance liquid chromatography (RP-HPLC) on a C18 column (250 × 4.6 mm, 5 μm) at a flow rate 0.3 ml/min, with elution using a 10–90% gradient of acetonitrile (containing 0.1% (v/v) trifluoroacetic acid) for 85 min. ( C ) The HPLC fractions were assayed for MMP-9 induction. ( D ) HPLC fractions with maximal MMP-9 activity (51 to 59 min) were used for identification of MMP-9 inducer and were subjected to 16.5% Tricine-SDS-PAGE gels. Defensin-1 (5.5 kDa) was detected by Western blotting using a rabbit polyclonal anti-honeybee defensin-1 antibody diluted 1:2000 in blocking buffer. Horseradish peroxidise-conjugated secondary antibodies were applied. (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S3 ). White line in gel indicates the place where two gels were spliced together. Data are expressed as means and SEMs of three independent measurements. Asterisks indicate a significant difference from the untreated group, * P

    Article Snippet: Rabbit polyclonal anti-bee defensin-1 (Def-1) was purchased from GenCust Europe (Luxembourg), and horseradish peroxidise-conjugated secondary antibodies were obtained from Promega (USA).

    Techniques: Purification, Incubation, Zymography, Activity Assay, High Performance Liquid Chromatography, Flow Cytometry, SDS Page, Western Blot, Blocking Assay

    Humoral immune response characterization. Absolute anti-αSyn IgM (A) and relative anti-αSyn IgM (B) antibodies in serum from immunized mice. Absolute anti-αSyn IgG (C) and relative anti-αSyn IgG (D) antibodies in serum. The relative content of specific anti α-Syn IgM and IgG antibodies were calculated by dividing anti α-Syn IgM or IgG levels by the total corresponding antibody levels. AU: arbitrary units. Represented values are mean ± S.E.M. ( n = 5–7). Asterisks correspond to statistically significant differences between one particular group and the “vehicle” group. Hash signs indicate statistically significant differences between different groups. * /# P

    Journal: Immunity, Inflammation and Disease

    Article Title: Chaperoned amyloid proteins for immune manipulation: α-Synuclein/Hsp70 shifts immunity toward a modulatory phenotype

    doi: 10.1002/iid3.39

    Figure Lengend Snippet: Humoral immune response characterization. Absolute anti-αSyn IgM (A) and relative anti-αSyn IgM (B) antibodies in serum from immunized mice. Absolute anti-αSyn IgG (C) and relative anti-αSyn IgG (D) antibodies in serum. The relative content of specific anti α-Syn IgM and IgG antibodies were calculated by dividing anti α-Syn IgM or IgG levels by the total corresponding antibody levels. AU: arbitrary units. Represented values are mean ± S.E.M. ( n = 5–7). Asterisks correspond to statistically significant differences between one particular group and the “vehicle” group. Hash signs indicate statistically significant differences between different groups. * /# P

    Article Snippet: After washing as in the previous step, wells were incubated for 1 h at 37°C with anti-IgM-(Miltenyi, Bergisch Gladbach, Germany) or anti-IgG- (Promega, Madison, WI, USA) -HRP conjugated secondary antibodies diluted 1:4000 in Assay Diluent (BD Biosciences, San Diego, CA, USA).

    Techniques: Mouse Assay