secondary antibodies peroxidase conjugated affinipure goat anti mouse igg  (Jackson Immuno)

 
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    Name:
    Peroxidase AffiniPure Goat Anti Mouse IgG Fcγ subclass 1 specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on antigen binding assay and or ELISA the antibody reacts with the Fc portion of mouse IgG1 but not with other mouse IgG subclasses mouse IgM or the Fab portion of mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human bovine and rabbit serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    115-035-205
    Price:
    228.0
    Category:
    Anti Mouse IgG Subclass Specific Antibodies
    Conjugate:
    Horseradish Peroxidase
    Size:
    0 5 ml
    Format:
    Whole IgG
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Jackson Immuno secondary antibodies peroxidase conjugated affinipure goat anti mouse igg
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on antigen binding assay and or ELISA the antibody reacts with the Fc portion of mouse IgG1 but not with other mouse IgG subclasses mouse IgM or the Fab portion of mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human bovine and rabbit serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/secondary antibodies peroxidase conjugated affinipure goat anti mouse igg/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary antibodies peroxidase conjugated affinipure goat anti mouse igg - by Bioz Stars, 2021-06
    93/100 stars

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    Incubation:

    Article Title: Immunolocalization of MMP9 and MMP2 in osteolytic metastasis originating from MDA-MB-231 human breast cancer cells
    Article Snippet: Subsequently, the sections were incubated with the following primary antibodies in BSA-PBS at room temperature for 2 h: SCGB2A2/mammaglobin A polyclonal antibody (MGB1; Proteintech; Sanying Biotechnology, Wuhan, China; cat. no. 235-1-AP; 1:50), anti-PCNA (Ab-1) mouse monoclonal antibody (PC10l; Epitomics; Abcam, Burlingame, CA, USA; cat. no. NA03; 1:50), mouse anti-human MMP2 monoclonal antibody (EMD Millipore, Billerica, MA, USA; cat. no. MAB3308; 1:50), mouse MMP9 antibody antigen affinifty-purified polyclonal goat IgG (EMD Millipore; cat. no. AF909; 1:50) and goat anti-MMP-13 polyclonal antibody (EMD Millipore; cat. no. AB8120; 1:50). .. Following rinsing with PBS, the sections were incubated with the following secondary antibodies for 1 h at room temperature: Polyclonal swine anti-rabbit immunoglobulin(Ig)/HRP from DakoCytomation, Denmark (cat. no. Nr.P 0399; 1:100), goat polyclonal anti-mouse IgG+IgM+IgA-H & L (HRP) from Abcam (cat. no. ab102448; 1:100), goat polyclonal anti-mouse IgG+IgM+IgA-H & L (HRP) from Abcam (cat. no. ab102448; 1:100), peroxidase-conjugated AffiniPure anti-goat++IgG (H+L) from Jackson Immunoresearch Laboratories, Inc. (West Grove, PA, USA; cat. no. 93894; 1:100) and peroxidase-conjugated AffiniPure anti-goat++IgG (H+L) from Jackson Immunoresearch Laboratories, Inc. (cat. no. 93894; 1:100). .. The immune complexes were then visualized using 3,3′-diamino-benzidine tetrahydrochloride (Sigma-Aldrich, St. Louis, MO, USA) as the substrate.

    Article Title: Human cytomegalovirus pp65 peptide-induced autoantibodies cross-reacts with TAF9 protein and induces lupus-like autoimmunity in BALB/c mice
    Article Snippet: For the competitive assays, 2 μg/well HCMVpp65422-439 , TAF9134-144 , dsDNA or TAF9 protein as competitor was mixed with 1 μg purified anti-HCMVpp65422-439 , anti-TAF9134-144 IgG, anti-TAF9 antibody or 100 μl eluted anti-TAF9 IgG fraction (1 ml/tube) with sterile PBS up to the final volume of 250 μl and added into peptide coating well for incubation at 37 °C for 2 hours. .. Following incubation, the microtiter plate was washed four times with TBST (TBS with 0.05% Tween-20) and any bound antibody was detected by horseradish peroxidase (HRP) conjugated anti-human/mouse IgG or anti-mouse IgG subclasses at 1:5,000 dilution (Jackson ImmunoResearch; Catalog code 109-035-088, 115-035-166, 115-035-205, 115-035-206, 115-035-207, 115-035-209) at 37 °C for 2 hours. .. After washing, TMB (Sigma-Aldrich) was used as the substrate, and HRP activity was measured at 450 nm by a microplate ELISA reader (EZ read 400).

    Article Title: Soluble Spike DNA vaccine provides long-term protective immunity against SAR-CoV-2 in mice and nonhuman primates
    Article Snippet: Sera or BAL fluid were serially diluted in 5% SM, added to the wells and incubated for 2 hours at 37°C. .. Following incubation, plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205), or IgG2a (Jackson ImmunoResearch Laboratories 115-035-206) or IgG2b (Jackson ImmunoResearch Laboratories 115-035-207) for the mouse sera/BAL or anti-monkey IgG (Bethyl Laborabories A140-102P) for the NHP sera for 1 hour at 37°C. .. After final wash plates were developed using TMB solution (Surmodics TMBW-0100-01) and the reaction stopped with 2N H2 SO4.

    Article Title: Development of an anti‐BAG3 humanized antibody for treatment of pancreatic cancer
    Article Snippet: .. Plates were then extensively washed and incubated 30 minutes at room temperature with HRP‐conjugated anti‐mouse IgGs 1 : 2000 (115‐035‐205, Jackson ImmunoResearch, Cambridgeshire, UK) or anti‐human IgG 1 : 20 000 (A0170, Sigma‐Aldrich). .. Subsequently, TMB solution 1X (eBioscience, San Diego, CA, USA) was added to the wells for the analyte detection.

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  • 94
    Jackson Immuno peroxidase conjugated goat anti mouse secondary antibody
    Theaflavins inhibit cell-to-cell spread. Huh-7 cells were inoculated with HCV JFH-1 for 2 h. Inoculum was replaced by medium containing mAb 3/11 neutralizing antibody to prevent extracellular propagation. Theaflavins at 25 μg/ml or EGCG at 50 μM were added in the medium after inoculation. Cells were fixed 72 h after infection and subjected to immunofluorescence detection of E1 HCV envelope protein revealed with a <t>Cy3-conjugated</t> <t>goat</t> <t>anti-mouse</t> <t>secondary</t> antibody (red). Nuclei were stained with DAPI (blue). Representative images of each condition are shown. Graph represents the number of cells/focus of 3 independent fields of three independent wells. Error bars represent SD. Data are representative of 3 independent experiments. Statistical analysis were performed with the Kruskal Wallis nonparametric test followed by a Dunn’s multicomparison post hoc test. **, P = 0.009; ***, P
    Peroxidase Conjugated Goat Anti Mouse Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti mouse secondary antibody/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti mouse secondary antibody - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    96
    Jackson Immuno goat anti mouse igg1 horseradish peroxidase conjugated antibody
    Functional evaluation of ICM in vitro and in vivo. A) Complement C1q binding ELISA; ICM were used at the 1∶20 antibody-antigen ratio and the neat sample contained 5 µg/m total protein (ICM) or the equivalent amount for individual components; each bar represents mean value from triplicate assays and the patterns indicate serial dilutions. B) Analysis of binding of ICM to spleen-derived APCs by flow cytometry; shown are the proportions of cells (out of 10,000 counted) that bound either mAb alone or ICM. C) Serum anti-Ag85B <t>IgG</t> responses from mice immunised with an equimolar (1∶1) or a low (1∶20) antibody-antigen ratio, twice at the base of the tail, at 3-week intervals. Mice were culled 3 weeks after the final immunisation. Shown are the mean values and corresponding serial dilutions from a pilot experiment (n = 3 mice).
    Goat Anti Mouse Igg1 Horseradish Peroxidase Conjugated Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg1 horseradish peroxidase conjugated antibody/product/Jackson Immuno
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg1 horseradish peroxidase conjugated antibody - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    Image Search Results


    Theaflavins inhibit cell-to-cell spread. Huh-7 cells were inoculated with HCV JFH-1 for 2 h. Inoculum was replaced by medium containing mAb 3/11 neutralizing antibody to prevent extracellular propagation. Theaflavins at 25 μg/ml or EGCG at 50 μM were added in the medium after inoculation. Cells were fixed 72 h after infection and subjected to immunofluorescence detection of E1 HCV envelope protein revealed with a Cy3-conjugated goat anti-mouse secondary antibody (red). Nuclei were stained with DAPI (blue). Representative images of each condition are shown. Graph represents the number of cells/focus of 3 independent fields of three independent wells. Error bars represent SD. Data are representative of 3 independent experiments. Statistical analysis were performed with the Kruskal Wallis nonparametric test followed by a Dunn’s multicomparison post hoc test. **, P = 0.009; ***, P

    Journal: PLoS ONE

    Article Title: Theaflavins, polyphenols of black tea, inhibit entry of hepatitis C virus in cell culture

    doi: 10.1371/journal.pone.0198226

    Figure Lengend Snippet: Theaflavins inhibit cell-to-cell spread. Huh-7 cells were inoculated with HCV JFH-1 for 2 h. Inoculum was replaced by medium containing mAb 3/11 neutralizing antibody to prevent extracellular propagation. Theaflavins at 25 μg/ml or EGCG at 50 μM were added in the medium after inoculation. Cells were fixed 72 h after infection and subjected to immunofluorescence detection of E1 HCV envelope protein revealed with a Cy3-conjugated goat anti-mouse secondary antibody (red). Nuclei were stained with DAPI (blue). Representative images of each condition are shown. Graph represents the number of cells/focus of 3 independent fields of three independent wells. Error bars represent SD. Data are representative of 3 independent experiments. Statistical analysis were performed with the Kruskal Wallis nonparametric test followed by a Dunn’s multicomparison post hoc test. **, P = 0.009; ***, P

    Article Snippet: Peroxidase-conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch) was used for the revelation using ECL western blotting substrate (Thermo Fischer Scientific).

    Techniques: Infection, Immunofluorescence, Staining

    TGF-β1 and TGF-β receptor 1 and 2 mRNA levels are strongly increased in microglia surrounding plaques of CCR2-deficient APP Swe /PS1 mice. Representative dark-field photomicrographs of in situ hybridization showing the cortical expression of TGF-β1 ( a , b ), TFG-β-R1 ( d , e ), and TGF-β-R2 mRNA ( g , h ) in the brain of 9-month-old APP Swe /PS1 (left column) and APP Swe /PS1/CCR2 −/− mice (middle column). Brain sections of 12-month-old APP Swe /PS1/CCR2 −/− mice were stained with an anti-iba1 antibody and peroxidase-conjugated secondary antibody, and thereafter hybridized with a mouse TGF-β1 ( c ), TGF-β-R1 ( f ), or TGF-β-R2 ( i ) cRNA probe. Each transcript (agglomeration of silver grains) is clearly expressed in microglia associated to plaques (brown cells). Qualitative quantification was performed for each transcript in the whole brain of 3- to 12-month-old APP Swe /PS1 and APP Swe /PS1/CCR2 −/− mice ( n = 5–10 per group). CCR2 deficiency clearly increased microglial expression of TGF-β1 and TGF-β-R2 around Aβ plaques of APP Swe /PS1 mice. Scale bars: Left and middle panels, 500 μm; right panel, 20 μm.

    Journal: The Journal of Neuroscience

    Article Title: CC Chemokine Receptor 2 Deficiency Aggravates Cognitive Impairments and Amyloid Pathology in a Transgenic Mouse Model of Alzheimer's Disease

    doi: 10.1523/JNEUROSCI.0299-11.2011

    Figure Lengend Snippet: TGF-β1 and TGF-β receptor 1 and 2 mRNA levels are strongly increased in microglia surrounding plaques of CCR2-deficient APP Swe /PS1 mice. Representative dark-field photomicrographs of in situ hybridization showing the cortical expression of TGF-β1 ( a , b ), TFG-β-R1 ( d , e ), and TGF-β-R2 mRNA ( g , h ) in the brain of 9-month-old APP Swe /PS1 (left column) and APP Swe /PS1/CCR2 −/− mice (middle column). Brain sections of 12-month-old APP Swe /PS1/CCR2 −/− mice were stained with an anti-iba1 antibody and peroxidase-conjugated secondary antibody, and thereafter hybridized with a mouse TGF-β1 ( c ), TGF-β-R1 ( f ), or TGF-β-R2 ( i ) cRNA probe. Each transcript (agglomeration of silver grains) is clearly expressed in microglia associated to plaques (brown cells). Qualitative quantification was performed for each transcript in the whole brain of 3- to 12-month-old APP Swe /PS1 and APP Swe /PS1/CCR2 −/− mice ( n = 5–10 per group). CCR2 deficiency clearly increased microglial expression of TGF-β1 and TGF-β-R2 around Aβ plaques of APP Swe /PS1 mice. Scale bars: Left and middle panels, 500 μm; right panel, 20 μm.

    Article Snippet: Membranes were stripped in 25 m m glycine-HCl, pH 2.0, containing 1% SDS to detect β-actin using first a mouse β-actin antibody (MAB1501; 1:5000; Millipore Bioscience Research Reagents) and then a goat anti-mouse peroxidase-conjugated secondary antibody (1:1000; Jackson ImmunoResearch).

    Techniques: Mouse Assay, In Situ Hybridization, Expressing, Staining

    Functional evaluation of ICM in vitro and in vivo. A) Complement C1q binding ELISA; ICM were used at the 1∶20 antibody-antigen ratio and the neat sample contained 5 µg/m total protein (ICM) or the equivalent amount for individual components; each bar represents mean value from triplicate assays and the patterns indicate serial dilutions. B) Analysis of binding of ICM to spleen-derived APCs by flow cytometry; shown are the proportions of cells (out of 10,000 counted) that bound either mAb alone or ICM. C) Serum anti-Ag85B IgG responses from mice immunised with an equimolar (1∶1) or a low (1∶20) antibody-antigen ratio, twice at the base of the tail, at 3-week intervals. Mice were culled 3 weeks after the final immunisation. Shown are the mean values and corresponding serial dilutions from a pilot experiment (n = 3 mice).

    Journal: PLoS ONE

    Article Title: Immune-Complex Mimics as a Molecular Platform for Adjuvant-Free Vaccine Delivery

    doi: 10.1371/journal.pone.0060855

    Figure Lengend Snippet: Functional evaluation of ICM in vitro and in vivo. A) Complement C1q binding ELISA; ICM were used at the 1∶20 antibody-antigen ratio and the neat sample contained 5 µg/m total protein (ICM) or the equivalent amount for individual components; each bar represents mean value from triplicate assays and the patterns indicate serial dilutions. B) Analysis of binding of ICM to spleen-derived APCs by flow cytometry; shown are the proportions of cells (out of 10,000 counted) that bound either mAb alone or ICM. C) Serum anti-Ag85B IgG responses from mice immunised with an equimolar (1∶1) or a low (1∶20) antibody-antigen ratio, twice at the base of the tail, at 3-week intervals. Mice were culled 3 weeks after the final immunisation. Shown are the mean values and corresponding serial dilutions from a pilot experiment (n = 3 mice).

    Article Snippet: The primary antibody for Acr was TBG65 mAb and the secondary antibody was a goat anti-mouse IgG1 horseradish peroxidase-conjugated antibody (1∶1000; Jackson Immuno Research); for Ag85B, a rabbit polyclonal anti-Ag85B antibody (1∶1000) was used, followed by a donkey anti-rabbit monoclonal antibody conjugated to horseradish peroxidase (1∶1000; Jackson Immuno Research).

    Techniques: Functional Assay, In Vitro, In Vivo, Binding Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay, Flow Cytometry, Mouse Assay

    rLCMV vector characterization. (A) Growth kinetics of rLCMV vectors and LCMV wild-type virus in the production cell line 293-GP. Titers represent means of 2 independent experiments, with standard deviations shown as error bars. (B) rLCMV-gB(dCt) was selected as the representative vector to demonstrate deficiency in formation of infectious progeny in cells that do not provide LCMV GP protein in trans (293F). All cell types were infected with 0.001 FFU/cell. Samples for individual time points were analyzed by means of a FFU assay based on LCMV GP-complementing 293T cells. Dotted lines indicate detection limits of the FFU assay. (C) gB(dCt) and pp65 vaccine antigen expression levels were analyzed by Western blotting. HCMV gB and HCMV pp65-specific MAbs were used to detect vaccine antigen expression, and a polyclonal anti-LCMV serum reactive with LCMV NP was used for the infection/loading control.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Additive Protection against Congenital Cytomegalovirus Conferred by Combined Glycoprotein B/pp65 Vaccination Using a Lymphocytic Choriomeningitis Virus Vector

    doi: 10.1128/CVI.00300-16

    Figure Lengend Snippet: rLCMV vector characterization. (A) Growth kinetics of rLCMV vectors and LCMV wild-type virus in the production cell line 293-GP. Titers represent means of 2 independent experiments, with standard deviations shown as error bars. (B) rLCMV-gB(dCt) was selected as the representative vector to demonstrate deficiency in formation of infectious progeny in cells that do not provide LCMV GP protein in trans (293F). All cell types were infected with 0.001 FFU/cell. Samples for individual time points were analyzed by means of a FFU assay based on LCMV GP-complementing 293T cells. Dotted lines indicate detection limits of the FFU assay. (C) gB(dCt) and pp65 vaccine antigen expression levels were analyzed by Western blotting. HCMV gB and HCMV pp65-specific MAbs were used to detect vaccine antigen expression, and a polyclonal anti-LCMV serum reactive with LCMV NP was used for the infection/loading control.

    Article Snippet: Antibody responses against HCMV gB in mouse sera were measured by indirect ELISA using recombinant gB (Sino Biological) as coating antigen on 96-well microplates (0.5 μg/ml, 100 μl per well, overnight at 4°C) and a goat HRP-conjugated anti-mouse IgG polyclonal secondary antibody (Jackson ImmunoResearch).

    Techniques: Plasmid Preparation, Infection, Expressing, Western Blot