Structured Review

Bio-Rad secondary antibodies conjugated to hrp
Secondary Antibodies Conjugated To Hrp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibodies conjugated to hrp/product/Bio-Rad
Average 90 stars, based on 11 article reviews
Price from $9.99 to $1999.99
secondary antibodies conjugated to hrp - by Bioz Stars, 2020-07
90/100 stars

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Related Articles

Staining:

Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
Article Snippet: .. For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA). .. Embryos were mounted between cover slips for viewing and analysed using Zeiss Axioplan 2 microscope (Thornwood, NY, USA).

Incubation:

Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
Article Snippet: .. For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA). .. Embryos were mounted between cover slips for viewing and analysed using Zeiss Axioplan 2 microscope (Thornwood, NY, USA).

Mouse Assay:

Article Title: Cardiac-Oxidized Antigens Are Targets of Immune Recognition by Antibodies and Potential Molecular Determinants in Chagas Disease Pathogenesis
Article Snippet: .. Membranes were probed with sera from normal or chagasic rats, mice or human patients (1∶100 dilution) followed by HRP-conjugated secondary antibody (1∶5000, BioRad), and signal was detected by an ECL plus chemiluminiscence detection system (GE-Healthcare). .. Image analysis 2D gels (n = 4/group) or Western blots (n = 4/group) were digitalized on a ProXPRESS Proteomic Imaging System (Perkin Elmer), and the images were analyzed on Progenesis SameSpotst™ software 2.0 (NonLinear Dynamics).

Conjugation Assay:

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: .. Detection was performed by adding HRP-conjugated Type 3 detection antibodies (conjugated with LYNX rapid HRP antibody conjugation kit, Bio-Rad LNK002P) or by adding Type 3 antibodies followed by HRP-conjugated anti-tag secondary antibodies in HISPEC assay diluent (Bio-Rad BUF49A) for 1 h at RT. .. Plates were washed ten times with PBST, followed by the addition of QuantaBlu fluorogenic peroxidase substrate (Thermo Fisher Scientific #15169).

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  • 92
    Bio-Rad hrp conjugated anti mouse secondary antibody
    Western blot analysis. Ten micrograms of parasite material from asexual blood stages, ookinete-enriched culture and mosquito midguts and salivary glands stages of PbGFP CON (lane 1) and pbht-gfp line (lane 2) was subjected to SDS-PAGE (10% acrylamide), transferred to a nitrocellulose membrane and probed with 1:1000 diluted anti-GFP antibody (Roche) and <t>1:3000</t> diluted <t>HRP-conjugated</t> anti-mouse antibody. *Ookinete-enriched culture obtained by incubation of blood sample of an infected mouse in the ookinete medium overnight at 19°C. p.i., post infection.
    Hrp Conjugated Anti Mouse Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated anti mouse secondary antibody/product/Bio-Rad
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated anti mouse secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    99
    Bio-Rad hrp conjugated secondary antibody
    Oxidative adducts are enhanced in experimental and human myocardium during Chagas disease. ( A ) Sprague-Dawley rats (or C3H/HeN mice) were infected with T. cruzi, and cells were harvested at day 40 (acute stage) and 180 (chronic stage) post-infection. Heart homogenates were resolved on 10% acrylamide gels, and Western blotting was performed with specific antibodies to detect 4 hydroxynonenal (4-HNE, panel a ), malondialdehyde (MDA, panel b ), dinitrophenyl (DNP)-derivatized carbonyl ( panel c ), and 3-nitrotyrosine (3NT, panel d ) adducts. Coomassie blue staining of membranes ( panel e ) confirmed the equal loading of samples. ( B ) Cryostat sections of human cardiac biopsies (5-µm) from normal healthy donors ( panels a, c, e ) and <t>chagasic</t> patients ( panels b, d, f ) were submitted to immunohistochemistry as described in Materials and Methods . Shown are representative images of immunostaining with anti-4-HNE antibodies ( panels a, b ). Tissue sections were incubated with DNPH to derivatize carbonyl proteins, and immunostaining was performed with anti-DNP antibody ( panels c–f ). <t>HRP-conjugated</t> ( panels a–d ) and rhodamine-conjugated ( panels e, f ) secondary antibodies were utilized to capture the color (brown) or fluorescence signal, respectively.
    Hrp Conjugated Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated secondary antibody/product/Bio-Rad
    Average 99 stars, based on 302 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated secondary antibody - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    Bio-Rad hrp conjugated goat anti rabbit secondary antibody
    Selective binding of the C1 fusion protein of <t>IC140</t> to axonemes lacking the I1 complex. Increasing amounts of purified fusion protein were mixed and incubated with isolated axonemes from either pf28pf30, lacking the I1 complex (lanes a–d), or pf28 (lanes e–h) (see MATERIALS AND METHODS). (A) Western blots using anti-IC140 serum, which reveals the 53-kDa fusion protein (double arrowhead) and IC140 (arrowhead) and using <t>HRP</t> secondary antibodies and developed with chemiluminescent reagents. (B) Duplicate gel stained with Commasie brilliant blue, confirming equal loads for each sample.
    Hrp Conjugated Goat Anti Rabbit Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit secondary antibody/product/Bio-Rad
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    95
    Bio-Rad horseradish peroxidase conjugated goat anti mouse
    Phospholipid scramblase is required for phosphatidylserine and Akt relocalization. (A) CaSki cells were mock-infected or infected with HSV-2(G) and 15 minutes, 30 minutes, 1 hour and 4 hours post-infection, cell lysates were harvested. Lysates were incubated with a <t>goat</t> <t>anti-PLSCR1</t> antibody and immune complexes precipitated with protein A-agarose and analyzed by Western blotting with a <t>mouse</t> anti-phosphotyrosine (PY20) or mouse anti-PLSCR1 mAb. The blot is representative of results obtained in 2 independent experiments. (B). CaSki, VK2E6/E7 or HaCAT cells were transfected with siRNA targeting PLSCR1 or a control siRNA (siCtrl) and protein expression was evaluated by Western blot probing for PLSCR1 (rabbit anti-PLSCR1) and β-actin (mouse monoclonal). Blot is representative of results obtained in 3 independent experiments. (C) CaSki or HaCAT cells were transfected with siControl (siCtrl) or siPLSCR1 RNA and 72 h post-transfection, plasma membranes were stained with Alexa Fluor <t>594-conjugated</t> wheat germ agglutinin (red) and then synchronously infected with HSV-1(KOS), HSV-2(G), or mock-infected (4 hours at 4°C, washed, and then shifted to 37°C for 15 min and treated with low pH citrate buffer). The cells were then fixed and nuclei were stained blue with Hoechst, and phosphatidylserines (PtdS) or PLSCR1 stained green with respective primary murine and secondary Alexa 488-conjugated secondary antibodies. Images are representative of results obtained from 2–3 independent experiments. (D). CaSki cells were transfected with the indicated siRNA and then synchronously infected with HSV-2(G), fixed with or without Triton X permeabilization and stained with fluorescently-conjugated antibodies to Akt (red) and PLSCR1 (green); nuclei were stained blue with DAPI. Representative 3-D images from 3 independent experiments are shown; bars = 10μm.
    Horseradish Peroxidase Conjugated Goat Anti Mouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated goat anti mouse/product/Bio-Rad
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated goat anti mouse - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    Western blot analysis. Ten micrograms of parasite material from asexual blood stages, ookinete-enriched culture and mosquito midguts and salivary glands stages of PbGFP CON (lane 1) and pbht-gfp line (lane 2) was subjected to SDS-PAGE (10% acrylamide), transferred to a nitrocellulose membrane and probed with 1:1000 diluted anti-GFP antibody (Roche) and 1:3000 diluted HRP-conjugated anti-mouse antibody. *Ookinete-enriched culture obtained by incubation of blood sample of an infected mouse in the ookinete medium overnight at 19°C. p.i., post infection.

    Journal: Molecular Microbiology

    Article Title: Life cycle studies of the hexose transporter of Plasmodium species and genetic validation of their essentiality

    doi: 10.1111/j.1365-2958.2010.07060.x

    Figure Lengend Snippet: Western blot analysis. Ten micrograms of parasite material from asexual blood stages, ookinete-enriched culture and mosquito midguts and salivary glands stages of PbGFP CON (lane 1) and pbht-gfp line (lane 2) was subjected to SDS-PAGE (10% acrylamide), transferred to a nitrocellulose membrane and probed with 1:1000 diluted anti-GFP antibody (Roche) and 1:3000 diluted HRP-conjugated anti-mouse antibody. *Ookinete-enriched culture obtained by incubation of blood sample of an infected mouse in the ookinete medium overnight at 19°C. p.i., post infection.

    Article Snippet: Bound antibodies were detected with a HRP-conjugated anti-mouse secondary antibody, diluted 1:3000 (Bio-Rad) and ECL Western Blotting detection reagents (Amersham).

    Techniques: Western Blot, SDS Page, Incubation, Infection

    Oxidative adducts are enhanced in experimental and human myocardium during Chagas disease. ( A ) Sprague-Dawley rats (or C3H/HeN mice) were infected with T. cruzi, and cells were harvested at day 40 (acute stage) and 180 (chronic stage) post-infection. Heart homogenates were resolved on 10% acrylamide gels, and Western blotting was performed with specific antibodies to detect 4 hydroxynonenal (4-HNE, panel a ), malondialdehyde (MDA, panel b ), dinitrophenyl (DNP)-derivatized carbonyl ( panel c ), and 3-nitrotyrosine (3NT, panel d ) adducts. Coomassie blue staining of membranes ( panel e ) confirmed the equal loading of samples. ( B ) Cryostat sections of human cardiac biopsies (5-µm) from normal healthy donors ( panels a, c, e ) and chagasic patients ( panels b, d, f ) were submitted to immunohistochemistry as described in Materials and Methods . Shown are representative images of immunostaining with anti-4-HNE antibodies ( panels a, b ). Tissue sections were incubated with DNPH to derivatize carbonyl proteins, and immunostaining was performed with anti-DNP antibody ( panels c–f ). HRP-conjugated ( panels a–d ) and rhodamine-conjugated ( panels e, f ) secondary antibodies were utilized to capture the color (brown) or fluorescence signal, respectively.

    Journal: PLoS ONE

    Article Title: Cardiac-Oxidized Antigens Are Targets of Immune Recognition by Antibodies and Potential Molecular Determinants in Chagas Disease Pathogenesis

    doi: 10.1371/journal.pone.0028449

    Figure Lengend Snippet: Oxidative adducts are enhanced in experimental and human myocardium during Chagas disease. ( A ) Sprague-Dawley rats (or C3H/HeN mice) were infected with T. cruzi, and cells were harvested at day 40 (acute stage) and 180 (chronic stage) post-infection. Heart homogenates were resolved on 10% acrylamide gels, and Western blotting was performed with specific antibodies to detect 4 hydroxynonenal (4-HNE, panel a ), malondialdehyde (MDA, panel b ), dinitrophenyl (DNP)-derivatized carbonyl ( panel c ), and 3-nitrotyrosine (3NT, panel d ) adducts. Coomassie blue staining of membranes ( panel e ) confirmed the equal loading of samples. ( B ) Cryostat sections of human cardiac biopsies (5-µm) from normal healthy donors ( panels a, c, e ) and chagasic patients ( panels b, d, f ) were submitted to immunohistochemistry as described in Materials and Methods . Shown are representative images of immunostaining with anti-4-HNE antibodies ( panels a, b ). Tissue sections were incubated with DNPH to derivatize carbonyl proteins, and immunostaining was performed with anti-DNP antibody ( panels c–f ). HRP-conjugated ( panels a–d ) and rhodamine-conjugated ( panels e, f ) secondary antibodies were utilized to capture the color (brown) or fluorescence signal, respectively.

    Article Snippet: Membranes were probed with sera from normal or chagasic rats, mice or human patients (1∶100 dilution) followed by HRP-conjugated secondary antibody (1∶5000, BioRad), and signal was detected by an ECL plus chemiluminiscence detection system (GE-Healthcare).

    Techniques: Mouse Assay, Infection, Western Blot, Multiple Displacement Amplification, Staining, Immunohistochemistry, Immunostaining, Incubation, Fluorescence

    Selective binding of the C1 fusion protein of IC140 to axonemes lacking the I1 complex. Increasing amounts of purified fusion protein were mixed and incubated with isolated axonemes from either pf28pf30, lacking the I1 complex (lanes a–d), or pf28 (lanes e–h) (see MATERIALS AND METHODS). (A) Western blots using anti-IC140 serum, which reveals the 53-kDa fusion protein (double arrowhead) and IC140 (arrowhead) and using HRP secondary antibodies and developed with chemiluminescent reagents. (B) Duplicate gel stained with Commasie brilliant blue, confirming equal loads for each sample.

    Journal: Molecular Biology of the Cell

    Article Title: The Mr 140,000 Intermediate Chain of Chlamydomonas Flagellar Inner Arm Dynein Is a WD-Repeat Protein Implicated in Dynein Arm Anchoring

    doi:

    Figure Lengend Snippet: Selective binding of the C1 fusion protein of IC140 to axonemes lacking the I1 complex. Increasing amounts of purified fusion protein were mixed and incubated with isolated axonemes from either pf28pf30, lacking the I1 complex (lanes a–d), or pf28 (lanes e–h) (see MATERIALS AND METHODS). (A) Western blots using anti-IC140 serum, which reveals the 53-kDa fusion protein (double arrowhead) and IC140 (arrowhead) and using HRP secondary antibodies and developed with chemiluminescent reagents. (B) Duplicate gel stained with Commasie brilliant blue, confirming equal loads for each sample.

    Article Snippet: The membrane was blocked with 5% nonfat dry milk followed by incubation first with anti-IC140 antibody (1:3000–1:6000 dilution) and then with HRP-conjugated goat anti-rabbit secondary antibody (1:5000, Bio-Rad ).

    Techniques: Binding Assay, Purification, Incubation, Isolation, Western Blot, Staining

    Phospholipid scramblase is required for phosphatidylserine and Akt relocalization. (A) CaSki cells were mock-infected or infected with HSV-2(G) and 15 minutes, 30 minutes, 1 hour and 4 hours post-infection, cell lysates were harvested. Lysates were incubated with a goat anti-PLSCR1 antibody and immune complexes precipitated with protein A-agarose and analyzed by Western blotting with a mouse anti-phosphotyrosine (PY20) or mouse anti-PLSCR1 mAb. The blot is representative of results obtained in 2 independent experiments. (B). CaSki, VK2E6/E7 or HaCAT cells were transfected with siRNA targeting PLSCR1 or a control siRNA (siCtrl) and protein expression was evaluated by Western blot probing for PLSCR1 (rabbit anti-PLSCR1) and β-actin (mouse monoclonal). Blot is representative of results obtained in 3 independent experiments. (C) CaSki or HaCAT cells were transfected with siControl (siCtrl) or siPLSCR1 RNA and 72 h post-transfection, plasma membranes were stained with Alexa Fluor 594-conjugated wheat germ agglutinin (red) and then synchronously infected with HSV-1(KOS), HSV-2(G), or mock-infected (4 hours at 4°C, washed, and then shifted to 37°C for 15 min and treated with low pH citrate buffer). The cells were then fixed and nuclei were stained blue with Hoechst, and phosphatidylserines (PtdS) or PLSCR1 stained green with respective primary murine and secondary Alexa 488-conjugated secondary antibodies. Images are representative of results obtained from 2–3 independent experiments. (D). CaSki cells were transfected with the indicated siRNA and then synchronously infected with HSV-2(G), fixed with or without Triton X permeabilization and stained with fluorescently-conjugated antibodies to Akt (red) and PLSCR1 (green); nuclei were stained blue with DAPI. Representative 3-D images from 3 independent experiments are shown; bars = 10μm.

    Journal: PLoS Pathogens

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry

    doi: 10.1371/journal.ppat.1006766

    Figure Lengend Snippet: Phospholipid scramblase is required for phosphatidylserine and Akt relocalization. (A) CaSki cells were mock-infected or infected with HSV-2(G) and 15 minutes, 30 minutes, 1 hour and 4 hours post-infection, cell lysates were harvested. Lysates were incubated with a goat anti-PLSCR1 antibody and immune complexes precipitated with protein A-agarose and analyzed by Western blotting with a mouse anti-phosphotyrosine (PY20) or mouse anti-PLSCR1 mAb. The blot is representative of results obtained in 2 independent experiments. (B). CaSki, VK2E6/E7 or HaCAT cells were transfected with siRNA targeting PLSCR1 or a control siRNA (siCtrl) and protein expression was evaluated by Western blot probing for PLSCR1 (rabbit anti-PLSCR1) and β-actin (mouse monoclonal). Blot is representative of results obtained in 3 independent experiments. (C) CaSki or HaCAT cells were transfected with siControl (siCtrl) or siPLSCR1 RNA and 72 h post-transfection, plasma membranes were stained with Alexa Fluor 594-conjugated wheat germ agglutinin (red) and then synchronously infected with HSV-1(KOS), HSV-2(G), or mock-infected (4 hours at 4°C, washed, and then shifted to 37°C for 15 min and treated with low pH citrate buffer). The cells were then fixed and nuclei were stained blue with Hoechst, and phosphatidylserines (PtdS) or PLSCR1 stained green with respective primary murine and secondary Alexa 488-conjugated secondary antibodies. Images are representative of results obtained from 2–3 independent experiments. (D). CaSki cells were transfected with the indicated siRNA and then synchronously infected with HSV-2(G), fixed with or without Triton X permeabilization and stained with fluorescently-conjugated antibodies to Akt (red) and PLSCR1 (green); nuclei were stained blue with DAPI. Representative 3-D images from 3 independent experiments are shown; bars = 10μm.

    Article Snippet: The secondary antibodies for Western blots were horseradish peroxidase-conjugated goat anti-mouse (170–5047, Bio-Rad, Hercules, CA), goat anti-rabbit (170–5046, Bio-Rad), and donkey anti-goat 1:1000 (sc-2020, Santa Cruz).

    Techniques: Infection, Incubation, Western Blot, Transfection, Expressing, Staining