Journal: PLoS ONE

Article Title: Toxicity Assays in Nanodrops Combining Bioassay and Morphometric Endpoints

doi: 10.1371/journal.pone.0000163

Figure Lengend Snippet: The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with sciFlexarrayer robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.

Article Snippet: Cells were spotted using the sciFLEXARRAYER robot arrayer from Scienion AG (Berlin, Germany) as described previously .

Techniques: Cell Based Assay, Clone Assay, Staining